Access the full text.
Sign up today, get DeepDyve free for 14 days.
Ralph Gräsbegk, Clas Nordman, A. Chapelle (2009)
THE LEUCOCYTE-MITOGENIC EFFECT OF SERUM FROM RABBITS IMMUNIZED WITH HUMAN LEUCOCYTES.Acta medica Scandinavica, 175
N. Ling, E. Spices, K. James, N. Williamson (1965)
The Activation of Human Peripheral Lymphocytes by Products of StaphylococciBritish Journal of Haematology, 11
D. Inman, E. Cooper (1963)
ELECTRON MICROSCOPY OF HUMAN LYMPHOCYTES STIMULATED BY PHYTOHAEMAGGLUTININThe Journal of Cell Biology, 19
R. Dutton, J. Eady (1964)
AN IN VITRO SYSTEM FOR THE STUDY OF THE MECHANISM OF ANTIGENIC STIMULATION IN THE SECONDARY RESPONSE.Immunology, 7
J. Robbins (1964)
TISSUE CULTURE STUDIES OF THE HUMAN LYMPHOCYTE.Science, 146 3652
K. Carstairs (1962)
The human small lymphocyte: its possible pluripotential quality.Lancet, 1 7234
(1959)
~., Studies on the leucocyte-agglutination
A. Coulson, D. Chalmers (1964)
SEPARATION OF VIABLE LYMPHOCYTES FROM HUMAN BLOOD.Lancet, 1 7331
A. Fagraeus (1948)
Antibody Production in relation to the Development of Plasma Cells. In vivo and in vitro Experiments.Acta Medica Scandinavica, 130
M. Elves, G. Taylor, S. Roath, M. Israëls (1963)
The in vitro Production of Antibody Lymphocytes.The Lancet, 281
D. Quaglino, F. Hayhoe, R. Flemans (1962)
Cytochemical Observations on the Effect of PhytohÆmagglutinin in Short-Term Tissue CulturesNature, 196
Yasukazu Tanaka, Lois Epstein, George Brecher, Frederick Stohlman (1963)
TRANSFORMATION OF LYMPHOCYTES IN CULTURES OF HUMAN PERIPHERAL BLOOD.Blood, 22
J. Gowans, D. McGregor, D. Cowen, C. Ford (1962)
Initiation of Immune Responses by Small LymphocytesNature, 196
M. Landy, R. Sanderson, M. Bernstein, A. Jackson (1964)
Antibody Production by Leucocytes in Peripheral BloodNature, 204
June East, M. Delphine (1964)
PREVENTION OF WASTING IN MICE THYMECTOMIZED AT BIRTH AND THEIR SUBSEQUENT REJECTION OF ALLOGENEIC LEUKEMIC CELLS.Journal of the National Cancer Institute, 33
C. Nordman, A. Delachapelle, R. Graesbeck (2009)
THE INTERRELATIONS OF ERYTHROAGGLUTINATING, LEUCOAGGLUTINATING AND LEUCOCYTE-MITOGENIC ACTIVITIES IN PHASEOLUS VULGARIS PHYTOHAEMAGGLUTININ.Acta medica Scandinavica, 175
D. Rigas, E. Johnson (1964)
STUDIES ON THE PHYTOHEMAGGLUTININ OF PHASEOLUS VULGARIS AND ITS MITOGENICITY *Annals of the New York Academy of Sciences, 113
S. Dubiski, Z. Dudziak, D. Skałba (1959)
Serum groups in rabbits.Immunology, 2 1
E. Delorme (1961)
Recovery from lethal irradiation in rats after intravenous administration of isogenic lymphocytes.Lancet, 2 7207
J. Gowans, E. Knight (1964)
The route of re-circulation of lymphocytes in the ratProceedings of the Royal Society of London. Series B. Biological Sciences, 159
F. Bach, K. Hirschhorn (1963)
γ-Globulin production by human lymphocytes in vitroExperimental Cell Research, 32
Richard Baney, Jacinto Vazquez, F. Dixon (1962)
Cellular Proliferation in Relation to Antibody Synthesis.∗ † ‡Proceedings of the Society for Experimental Biology and Medicine, 109
(1964)
Electron microscopy studies of lymphocytes
R. Billingham, V. Defendi, W. Silvers, D. Steinmuller (1962)
Quantitative studies on the induction of tolerance of skin homografts and on runt disease in neonatal rats.Journal of the National Cancer Institute, 28
P. Nowell (1960)
Phytohemagglutinin: an initiator of mitosis in cultures of normal human leukocytes.Cancer research, 20
A. Rubin, K. Stenzel, K. Hirschhorn, F. Bach (1964)
Histocompatibility and Immunologic Competence in Renal HomotransplantationScience, 143
J. Gowans (1962)
THE FATE OF PARENTAL STRAIN SMALL LYMPHOCYTES IN F1 HYBRID RATSAnnals of the New York Academy of Sciences, 99
G. Nossal, O. Mäkelä (1962)
AUTORADIOGRAPHIC STUDIES ON THE IMMUNE RESPONSEThe Journal of Experimental Medicine, 115
Barbara Bain, L. Lowenstein (1964)
Genetic Studies on the Mixed Leukocyte ReactionScience, 145
Barbara Bain, M. Vas, L. Lowenstein (1964)
THE DEVELOPMENT OF LARGE IMMATURE MONONUCLEAR CELLS IN MIXED LEUKOCYTE CULTURES.Blood, 23
J. Miller (1964)
THE THYMUS AND THE DEVELOPMENT OF IMMUNOLOGIC RESPONSIVENESS.Science, 144 3626
R. Kolodny, K. Hirschhorn (1964)
Properties of PhytohæmagglutininNature, 201
S. Knight, N. Ling, S. Sell, C. Oxnard (1965)
The transformation in vitro of peripheral lymphocytes of some laboratory animals.Immunology, 9 6
(1963)
The origin of antibody forming cells, Immunopathol
(1958)
The lymphocyte , Internat
K. Lindahl-Kiessling, B. Werner, J. Böök (2009)
Short term cultivation of human thoracic duct lymphocytes with Phaseolus vulgaris extract.Hereditas, 53 1
S. Dray, S. Dubiski, A. Kelus, E. Lennox, J. Oudin (1962)
A Notation for AllotypyNature, 195
Rabbit lymphocytes may be stimulated in vitro with specific antiallotype sera to transform into "blast" cells and to synthesize DNA. This transformation only occurs when the donor cells are obtained from a rabbit having a given γ-globulin allotype (As4) and these cells are cultured in the presence of an antiserum prepared against the given allotype (As4). Heterologous (sheep, goat, and guinea pig) anti-rabbit γ-globulin sera also induce significant blast transformation and DNA synthesis in rabbit lymphocytes. Allotypic transformation and DNA synthesis are due to 7S antiallotype antibodies and do not require complement. The degree of transformation and rate of DNA synthesis is related to the concentration of antibody. Incubation of the appropriate cells with the antiallotype antibody for as short a time as 15 minutes results in a significant degree of "blast" transformation, indicating that the recognition of the antiallotype specificity in the cells and stimulation of the cellular changes leading to eventual transformation is rapid. The activity of the antiallotype sera as measured by transforming or haemagglutinating capacity, may be absorbed by lymphocytes of the appropriate allotype, but is not absorbed by lymphocytes from a donor rabbit not having the allotype to which the antiserum is directed. Transformation does not occur with mixtures of lymphocytes from different rabbits even if 1 donor is immunized against an allotype present in the other donor. Peripheral rabbit lymphocytes can also be induced to undergo "blast transformation" in vitro by phytohaemagglutinin and staphylococcal filtrate. The lack of demonstrable leucoagglutinins in staphylococcal filtrate and antiallotype serum indicates that agglutination is not a necessary prerequisite to the induction of blast transformation. Footnotes Submitted: 29 March 1965
The Journal of Experimental Medicine – Rockefeller University Press
Published: Aug 1, 1965
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.