Access the full text.
Sign up today, get DeepDyve free for 14 days.
K. Bailey (1948)
Tropomyosin: a new asymmetric protein component of the muscle fibril.The Biochemical journal, 43 2
F. Pepe (1963)
POSSIBILITIES FOR THE USE OF ANTIBODY STAINING TECHNIQUES IN ELECTRON MICROSCOPY
F. Carlsen, G. Knappeis, F. Buchthal (1961)
ULTRASTRUCTURE OF THE RESTING AND CONTRACTED STRIATED MUSCLE FIBER AT DIFFERENT DEGREES OF STRETCHThe Journal of Biophysical and Biochemical Cytology, 11
R. Rice (1961)
Conformation of individual macromolecular particles from myosin solutionsBiochimica et Biophysica Acta, 52
H. Huxley (1963)
ELECTRON MICROSCOPE STUDIES ON THE STRUCTURE OF NATURAL AND SYNTHETIC PROTEIN FILAMENTS FROM STRIATED MUSCLE.Journal of molecular biology, 7
F. Pepe (1961)
THE USE OF SPECIFIC ANTIBODY IN ELECTRON MICROSCOPYThe Journal of Biophysical and Biochemical Cytology, 11
Andrew Szent-Györgyi (1953)
Meromyosins, the subunits of myosin.Archives of biochemistry and biophysics, 42 2
Michael Reedy, Kenneth Holmes, Richard Tregear (1965)
Induced Changes in Orientation of the Cross-Bridges of Glycerinated Insect Flight MuscleNature, 207
C. Zobel, F. Carlson (1963)
An electron microscopic investigation of myosin and some of its aggregates.Journal of molecular biology, 7
(1964)
Structural evidence concerning the mechanism of contraction in striated muscle
S. Lowey, S. Lowey, C. Cohen, C. Cohen (1962)
Studies on the structure of myosin.Journal of molecular biology, 4
K. Bailey (1951)
End-group assay in some proteins of the keratin-myosin group.The Biochemical journal, 49 1
G. Elliott, J. Lowy, C. Worthington (1963)
An X-ray and light-diffraction study of the filament lattice of striated muscle in the living state and in rigorJournal of Molecular Biology, 6
(1954)
Interference microscopy of living muscle fibers
H. Huxley, J. Hanson (1954)
Changes in the Cross-Striations of Muscle during Contraction and Stretch and their Structural InterpretationNature, 173
C. Worthington (1961)
X-ray diffraction studies on the large-scale molecular structure of insect muscle.Journal of molecular biology, 3
G. Elliott, J. Lowy, B. Millman (1965)
X-ray Diffraction from Living Striated Muscle during ContractionNature, 206
J. Marshall, H. Holtzer, H. Finck, F. Pepe (1959)
The distribution of protein antigens in striated myofibrilsExperimental Cell Research, 7
W. Mommaerts, R. Parrish (1951)
Studies on myosin. I. Preparation and criteria of purity.The Journal of biological chemistry, 188 2
J. Hanson, J. Lowy (1963)
The structure of F-actin and of actin filaments isolated from muscleJournal of Molecular Biology, 6
D. Philpott, A. Szent-Györgyi (1954)
The structure of light-meromyosin: an electron microscopic study.Biochimica et biophysica acta, 15 2
B. Tunik, H. Holtzer (1961)
THE DISTRIBUTION OF MUSCLE ANTIGENS IN CONTRACTED MYOFIBRILS DETERMINED BY FLUORESCEIN-LABELED ANTIBODIESThe Journal of Biophysical and Biochemical Cytology, 11
(1964)
Antibody staining of separated thin and thick filaments of striated muscle, in Biochemistry of Muscle Contraction, (John Gergely, editor)
H. Deutsch (1952)
II. Separation of antibody-active proteins from various animal sera by ethanol fractionation techniques.Methods in medical research, 5
S. Perry, A. Corsi (1958)
Extraction of proteins other than myosin from the isolated rabbit myofibril.The Biochemical journal, 68 1
S. Page, H. Huxley (1963)
FILAMENT LENGTHS IN STRIATED MUSCLEThe Journal of Cell Biology, 19
(1964)
Fluorochromes and their conjugation with proteins
A study of actin by means of gel electrophoresis
(1964)
Study of localization of muscle proteins in the isolated myofibrils by the fluorescing antibody
E. Mihályi, A. Szent-Gyorgyi (1953)
Trypsin digestion of muscle proteins. I. Ultracentrifugal analysis of the process.The Journal of biological chemistry, 201 1
H. Huxley, W. Brown, K. Holmes (1965)
Constancy of Axial Spacings in Frog Sartorius Muscle during ContractionNature, 206
R. Davies (1963)
A Molecular Theory of Muscle Contraction : Calcium-Dependent Contractions with Hydrogen Bond Formation Plus ATP-Dependent Extensions of Part of the Myosin-Actin Cross-BridgesNature, 199
(1964)
Electron microscopy of macromolecules from myosin solutions, in Biochemistry of Muscle Contraction
E. Mihályi, A. Szent-Gyorgyi (1953)
Trypsin digestion of muscle proteins. III. Adenosinetriphosphatase activity and actinbinding capacity of the digested myosin.The Journal of biological chemistry, 201 1
A. Szent-Györgyi, C. Cohen, D. Philpott (1960)
Light meromyosin fraction I: A helical molecule from myosinJournal of Molecular Biology, 2
Antibody Staining of Muscle
K. Bailey (1946)
Tropomyosin: a New Asymmetric Protein Component of MuscleNature, 157
J. Hanson, J. Lowy (1964)
The structure of actin filaments and the origin of the axial periodicity in the I-substance of vertebrate striated muscleProceedings of the Royal Society of London. Series B. Biological Sciences, 160
W. Mommaerts (1952)
The molecular transformations of actin. I. Globular actin.The Journal of biological chemistry, 198 1
(1964)
Localization of myosin in chick myofibrils determined at various sarcomere lengths with the aid of antibody
From observations of fluorescent antibody staining and antibody staining in electron microscopy, evidence is presented for the following: ( a ) Direct contact of the actin and myosin filaments occurs at all stages of contraction. This results in inhibition of antibody staining of the H-meromyosin portion of the myosin molecule in the region of overlap of the thin and thick filaments. ( b ) Small structural changes occur in the thick filaments during contraction. This leads to exposure of antigenic sites of the L-meromyosin portion of the myosin molecule. The accessibility of these antigenic sites is dependent upon the sarcomere length. ( c ) The M line is composed of a protein which is weakly bound to the center of the thick filament and is not actin, myosin, or tropomyosin. ( d ) Tropomyosin as well as actin is present in the I band. ( e ) If actin or tropomyosin is present in the Z line, it is masked and unavailable for staining with antibody. Footnotes Submitted: 25 October 1965
The Journal of Cell Biology – Rockefeller University Press
Published: Mar 1, 1966
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.