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SOME ASPECTS OF THE STRUCTURAL ORGANIZATION OF THE MYOFIBRIL AS REVEALED BY ANTIBODY-STAINING METHODS

SOME ASPECTS OF THE STRUCTURAL ORGANIZATION OF THE MYOFIBRIL AS REVEALED BY ANTIBODY-STAINING... From observations of fluorescent antibody staining and antibody staining in electron microscopy, evidence is presented for the following: ( a ) Direct contact of the actin and myosin filaments occurs at all stages of contraction. This results in inhibition of antibody staining of the H-meromyosin portion of the myosin molecule in the region of overlap of the thin and thick filaments. ( b ) Small structural changes occur in the thick filaments during contraction. This leads to exposure of antigenic sites of the L-meromyosin portion of the myosin molecule. The accessibility of these antigenic sites is dependent upon the sarcomere length. ( c ) The M line is composed of a protein which is weakly bound to the center of the thick filament and is not actin, myosin, or tropomyosin. ( d ) Tropomyosin as well as actin is present in the I band. ( e ) If actin or tropomyosin is present in the Z line, it is masked and unavailable for staining with antibody. Footnotes Submitted: 25 October 1965 http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Cell Biology Rockefeller University Press

SOME ASPECTS OF THE STRUCTURAL ORGANIZATION OF THE MYOFIBRIL AS REVEALED BY ANTIBODY-STAINING METHODS

The Journal of Cell Biology , Volume 28 (3): 505 – Mar 1, 1966

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References (40)

Publisher
Rockefeller University Press
Copyright
© 1966 Rockefeller University Press
ISSN
0021-9525
eISSN
1540-8140
DOI
10.1083/jcb.28.3.505
Publisher site
See Article on Publisher Site

Abstract

From observations of fluorescent antibody staining and antibody staining in electron microscopy, evidence is presented for the following: ( a ) Direct contact of the actin and myosin filaments occurs at all stages of contraction. This results in inhibition of antibody staining of the H-meromyosin portion of the myosin molecule in the region of overlap of the thin and thick filaments. ( b ) Small structural changes occur in the thick filaments during contraction. This leads to exposure of antigenic sites of the L-meromyosin portion of the myosin molecule. The accessibility of these antigenic sites is dependent upon the sarcomere length. ( c ) The M line is composed of a protein which is weakly bound to the center of the thick filament and is not actin, myosin, or tropomyosin. ( d ) Tropomyosin as well as actin is present in the I band. ( e ) If actin or tropomyosin is present in the Z line, it is masked and unavailable for staining with antibody. Footnotes Submitted: 25 October 1965

Journal

The Journal of Cell BiologyRockefeller University Press

Published: Mar 1, 1966

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