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SIRT1 deacetylates RORγt and enhances Th17 cell generation var callbackToken='50520277D3E02DA'; var subCode='jcb_sub'; var subScriptionType = 'author-datacenter'; MathJax.Hub.Config({ menuSettings: { zoom: "Click" }, tex2jax: { inlineMath: [["$","$"],["\\(","\\)"]], processClass: "tex2jax_process|mathjax" }, "HTML-CSS": { linebreaks: { automatic: true } } }); MathJax.Hub.Queue(function() { gColTempResize = true; fixColHeights(1); gColTempResize = false; }); MathJax.Hub.Register.MessageHook("math zoomed", function (message) { $(".mj").css("overflow", "visible"); $("#MathJax_Zoom").css("background-color", "#ffffff"); $("#MathJax_Zoom").css("font-size", "22px"); $("#MathJax_Zoom").css("max-height", "inherit"); $("#MathJax_ZoomFrame").css("background-color", "#ffffff"); $("#content-block").css("overflow", "visible"); }); MathJax.Hub.Register.MessageHook("math unzoomed", function (message) { $(".mj").css("overflow", "hidden"); $("#content-block").css("overflow", "hidden"); }); The Rockefeller University Press The Journal of Cell Biology The Journal of Experimental Medicine The Journal of General Physiology Institution: DeepDyve Sign In as Member / Individual about JEM meet our editors alerts & feeds permissions contact us subscribe submit HOME Current issue NEWEST ARTICLES REVIEWS & OPINION ARCHIVE Search JEM Full Text Authors Advanced Search JEM Home > 2015 Archive > 1 June > Lim et al. 212 (6): 973 Published May 18, 2015 // JEM vol. 212 no. 6 973 The Rockefeller University Press , doi: 10.1084/jem.2013237805062015c © 2015 Lim et al. Correction SIRT1 deacetylates RORγt and enhances Th17 cell generation Hyung W. Lim , Seung Goo Kang , Jae Kyu Ryu , Birgit Schilling , Mingjian Fei , Intelly S. Lee , Amanuel Kehasse , Kotaro Shirakawa , Masaru Yokoyama , Martina Schnölzer , Herbert G. Kasler , Hye-Sook Kwon , Bradford W. Gibson , Hironori Sato , Katerina Akassoglou , Changchun Xiao , Dan R. Littman , Melanie Ott , and Eric Verdin Vol. 212, No. 5, May 4, 2015. Pages 607–617 . The authors regret that the labels “Input” and “IP: α -Flag” were switched in the original publication of Fig. 2 (E and F) . This has now been corrected in the HTML and PDF versions of the paper. The corrected image also appears below. View larger version: In this window In a new window Download as PowerPoint Slide Figure 2. SIRT1 interacts with RORγt . (A) Flag-tagged WT or H363Y mutant SIRT1 was immunoprecipitated from transfected 293T cells and probed as indicated. (B) RORγt was immunoprecipitated from thymocytes and Th17 cells, and probed with antibody against SIRT1. (C) Immunoprecipitation using lysates of 293T cells co-transfected with constructs encoding SIRT1 and various deletion mutants of RORγt. Relative binding was calculated by normalizing the ratio of immunoprecipitated RORγt/SIRT1 to the ratio of input RORγt/SIRT1. (D) Acetylation of Flag-tagged RORγt immunoprecipitated from 293T cells transfected with various acetyltransferases and Flag-RORγt. (E and F) Acetylation of Flag-RORγt co-transfected with p300 and WT or H363Y mutant SIRT1, in the absence (E) or in the presence (F) of nicotinamide and Ex-527. Equal amounts of Flag-RORγt were loaded (D–F). Representative data are shown from four (A), three (B and E), and two (D) independent experiments, and combined data are shown from three (C and F) independent experiments with error bars representing ±SEM.
The Journal of Experimental Medicine – Rockefeller University Press
Published: Jun 1, 2015
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