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(1967)
Lymphocytotoxic microtechnique. Purification of lymphocytes by flotation
Darcy Wilson (1967)
QUANTITATIVE STUDIES ON THE MIXED LYMPHOCYTE INTERACTION IN RATSThe Journal of Experimental Medicine, 126
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Role of histone in chromosomal RNA synthesis
F. Kissmeyer-Nielsen, A. Svejgaard, M. Hauge (1968)
Genetics of the Human HL-A Transplantation SystemNature, 219
G. Zubay, P. Doty (1959)
The isolation and properties of deoxyribonucleoprotein particles containing single nucleic acid moleculesJournal of Molecular Biology, 1
M. Schwarz (1968)
THE MIXED LYMPHOCYTE REACTION: AN IN VITRO TEST FOR TOLERANCEThe Journal of Experimental Medicine, 127
L. Bolund, N. Ringertz, H. Harris (1969)
Changes in the cytochemical properties of erythrocyte nuclei reactivated by cell fusion.Journal of cell science, 4 1
D. Killander, Rudolf Rigler (1969)
Activation of deoxyribonucleoprotein in human leucocytes stimulated by phytohemagglutinin. I. Kinetics of the binding of acridine orange to deoxyribonucleoprotein.Experimental cell research, 54 2
G. Lundgren, G. Möller (1969)
Non-specific induction of cytotoxicity in normal human lymphocytes in vitro: studies of mechanism and specificity of the reaction.Clinical and experimental immunology, 4 4
A. Coulson, D. Chalmers (1964)
SEPARATION OF VIABLE LYMPHOCYTES FROM HUMAN BLOOD.Lancet, 1 7331
R. Rigler (1966)
Microfluorometric characterization of intracellular nucleic acids and nucleoproteins by acridine orange.Acta physiologica Scandinavica. Supplementum, 267
T. Caspersson, G. Lomakka (1966)
Microscale spectroscopy.Biochemical Society symposium, 26
Lars Bolund, Gösta Gahrton, D. Killander, R. Rigler, Britta Wahren (1970)
Structural changes in the deoxyribonucleoprotein (DNP) complex of leukocytes from patients with infectious mononucleosis.Blood, 35 3
C. Naspitz, M. Richter (1968)
The Action of Phytohemagglutinin in vivo and in vitro, a Review (Part 1 of 3), 12
R. Albertini, F. Bach (1968)
QUANTITATIVE ASSAY OF ANTIGENIC DISPARITY AT HL-A—THE MAJOR HISTOCOMPATIBILITY LOCUS IN MANThe Journal of Experimental Medicine, 128
E. Valenstein, V. Cox, J. Kakolewski (1967)
Leukotactic Factor Elaborated by Mixtures of Genetically Dissimilar CellsScience, 157
B. Pogo, V. Allfrey, A. Mirsky (1966)
RNA synthesis and histone acetylation during the course of gene activation in lymphocytes.Proceedings of the National Academy of Sciences of the United States of America, 55 4
(1966)
Changes in deoxyribonucleoprotein during spermatogenesis in bull
(1965)
Registrierender Fluoreszenzmikrospektrograph zur bestimmung der Primiir- und Sekund~irfluoreszenz verschidener Zellsubstanzen
Rudolf Rigler, D. Killander (1969)
Activation of deoxyribonucleoprotein in human leucocytes stimulated by phytohemagglutinin. II. Structural changes of deoxyribonucleoprotein and synthesis of RNA.Experimental cell research, 54 2
C. Naspitz, M. Richter (1968)
The Action of Phytohemagglutinin in vivo and in vitro, a ReviewProgress in allergy, 12
Zbigniew Darzynkiewicz, Lars Bolund, Nils Ringertz (1969)
Nucleoprotein changes and initiation of RNA synthesis in PHA stimulated lymphocytes.Experimental cell research, 56 2
Nils Ringertz, Lars Bolund (1969)
"Activation" of hen erythrocyte deoxyribonucleoprotein.Experimental cell research, 55 2
Barbara Bain, M. Vas, L. Lowenstein (1964)
THE DEVELOPMENT OF LARGE IMMATURE MONONUCLEAR CELLS IN MIXED LEUKOCYTE CULTURES.Blood, 23
Rudolf Rigler, D. Killander, Lars Bolund, Nils Ringertz (1969)
Cytochemical characterization of deoxyribonucleoprotein in individual cell nuclei. Techniques for obtaining heat denaturation curves with the aid of acridine orange microfluorimetry and ultraviolet microspectrophotometry.Experimental cell research, 55 2
(1963)
Immune response and mitosis of human peripheral blood lymphocytes in ~tro
D. Killander, Rudolf Rigler (1965)
Initial changes of deoxyribonucleoprotein and synthesis of nucleic acid in phytohemagglutinine-stimulated human leucocytes in vitro.Experimental cell research, 39 2
(1963)
A reaction between leukocytes in mixed peripheral blood cultures
K. Hellström, G. Möller (1965)
Immunological and Immunogenetic Aspects of Tumor Transplantation, 9
The mechanism of growth stimulation in allogeneic lymphocytes mixed in vitro was studied at the cell level by means of cytophotometric techniques. A pronounced increase in fluorescence intensity of fixed and acridine orange (AO) stained lymphocytes was observed as soon as after 1–3 hr in mixed culture. No increase in the amount of DNA took place during this time. The higher fluorescence intensity was due to an increased accessibility of AO binding sites in the deoxyribonucleoprotein (DNP) complex, most probably as a result of weakened bonds between the DNA and the protein moiety in the DNP complex. Similar DNP changes have been found in other systems of growth stimulation and may be one prerequisite for later induction of cellular synthetic processes. Increased AO binding only occurred when the lymphocyte donors were incompatible at the major histocompatibility locus (HL-A); there was no change in AO binding in cases of HL-A identity. The AO binding reaction probably reflects a specific recognition of HL-A antigens, whereas other antigenic discrepancies between the individuals do not seem to cause an analogous response. Footnotes Submitted: 19 September 1969
The Journal of Experimental Medicine – Rockefeller University Press
Published: Feb 1, 1970
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