Access the full text.
Sign up today, get DeepDyve free for 14 days.
H. Kalckar (1947)
Differential spectrophotometry of purine compounds by means of specific enzymes; determination of hydroxypurine compounds.The Journal of biological chemistry, 167 2
R. Dippell (1976)
Effects of nuclease and protease digestion on the ultrastructure of Paramecium basal bodiesThe Journal of Cell Biology, 69
(1980)
Spatial organization of microtubules
R. Anderson, R. Brenner (1971)
THE FORMATION OF BASAL BODIES (CENTRIOLES) IN THE RHESUS MONKEY OVIDUCTThe Journal of Cell Biology, 50
O. Sperling, A. Vries, J. Wyngaarden (1974)
Purine Metabolism in Man
E. Giblett, A. Ammann, R. Sandman, D. Wara, Louis Diamond (1975)
NUCLEOSIDE-PHOSPHORYLASE DEFICIENCY IN A CHILD WITH SEVERELY DEFECTIVE T-CELL IMMUNITY AND NORMAL B-CELL IMMUNITYThe Lancet, 305
H. Hartman, J. Puma, T. Gruney (1974)
Evidence for the association of RNA with the ciliary basal bodies of Tetrahymena.Journal of cell science, 16 2
Joe A, Connolly, Vitauts Kalnins (1978)
Visualization of centrioles and basal bodies by fluorescent staining with nonimmune rabbit seraThe Journal of Cell Biology, 79
N. Harboe, A. Ingild (1973)
23. Immunization, Isolation of Immunoglobulins, Estimation of Antibody TitreScandinavian Journal of Immunology, 2
(1980)
Microtubule-membrane interaction and the control of immune function: studies in normal and abnormal human lymphocytes
S. Heidemann, G. Sander, M. Kirschner (1977)
Evidence for a functional role of RNA in centriolesCell, 10
J. Oliver, E. Gelfand, C. Pearson, J. Pfeiffer, H. Dosch (1980)
Microtubule assembly and conanavalin A capping in lymphocytes: reappraisal using normal and abnormal human peripheral blood cells.Proceedings of the National Academy of Sciences of the United States of America, 77 6
J. Stoeckler, R. Agarwal, K. Agarwal, K. Schmid, R. Parks (1978)
Purine nucleoside phosphorylase from human erythrocytes: physiocochemical properties of the crystalline enzyme.Biochemistry, 17 2
C. Rieder (1979)
Ribonucleoprotein staining of centrioles and kinetochores in newt lung cell spindlesThe Journal of Cell Biology, 80
E. Becker (1972)
THE RELATIONSHIP OF THE CHEMOTACTIC BEHAVIOR OF THE COMPLEMENT-DERIVED FACTORS, C3a, C5a, AND C567, AND A BACTERIAL CHEMOTACTIC FACTOR TO THEIR ABILITY TO ACTIVATE THE PROESTERASE 1 OF RABBIT POLYMORPHONUCLEAR LEUKOCYTESThe Journal of Experimental Medicine, 135
(1980)
A microtubule assembly model
William Osborne, Shi-Han Chen, E. Giblett, W. Biggar, Arthur Ammann, C. Scott (1977)
Purine nucleoside phosphorylase deficiency. Evidence for molecular heterogeneity in two families with enzyme-deficient members.The Journal of clinical investigation, 60 3
W. Osborne (1980)
Human red cell purine nucleoside phosphorylase. Purification by biospecific affinity chromatography and physical properties.The Journal of biological chemistry, 255 15
T. Finlay, V. Troll, M. Levy, A. Johnson, L. Hodgins (1978)
New methods for the preparation of biospecific adsorbents and immobilized enzymes utilizing trichloro-s-triazine.Analytical biochemistry, 87 1
D. Pepper, B. Brinkley (1980)
Tubulin nucleation and assembly in mitotic cells: evidence for nucleic acids in kinetochores and centrosomes.Cell motility, 1 1
W. Osborne (1981)
Inherited absences of purine recycling enzymes associated with defects of immunityTrends in Biochemical Sciences, 6
R. Maccioni, N. Seeds (1977)
Stoichiometry of GTP hydrolysis and tubulin polymerization.Proceedings of the National Academy of Sciences of the United States of America, 74 2
M. Muller (1977)
Purine Metabolism in Man—II Regulation of Pathways and Enzyme Defects
E. Gelfand, H. Dosch (1978)
Biological Basis for ImmunodeficiencyScandinavian Journal of Immunology, 7
J. Henderson, A. Paterson (1973)
Nucleotide Metabolism: An Introduction
T. David‐Pfeuty, H. Erickson, D. Pantaloni (1977)
Guanosinetriphosphatase activity of tubulin associated with microtubule assembly.Proceedings of the National Academy of Sciences of the United States of America, 74 12
S. Peterson, M. Berns (1978)
Evidence for centriolar region RNA functioning in spindle formation in dividing PTK2 cells.Journal of cell science, 34
J. Virelizier, M. Hamet, J. Ballet, P. Reinert, C. Griscelli (1978)
Impaired defense against vaccinia in a child with T-lymphocyte deficiency associated with inosine phosphorylase defect.The Journal of pediatrics, 92 3
R. Berlin, J. Oliver, R. Walter (1978)
Surface functions during mitosis I: Phagocytosis, pinocytosis and mobility of surface-bound ConACell, 15
M. Borgers, H. Verhaegen, M. Brabander, F. Thoné, J. Reempts, G. Geuens (1977)
Purine nucleoside phosphorylase, a possible histochemical marker for T-cells in man.Journal of immunological methods, 16 2
William Osborne, C. Scott (1980)
Purine nucleoside phosphorylase deficiency. Measurement of variant protein in four families with enzyme-deficient members by an enzyme-linked immunosorbent assay.American journal of human genetics, 32 6
(1980)
Isolation of microtubule organizing centers from mouse neuroblastoma cells
J. Braun, F. Rosen, E. Unanue (1980)
Capping and adenosine metabolism. Genetic and pharmacologic studiesThe Journal of Experimental Medicine, 151
(1980)
Diseases of purine and pyrimidine metabolism
B. Telzer, J. Rosenbaum (1979)
Cell cycle-dependent, in vitro assembly of microtubules onto pericentriolar material of HeLa cellsThe Journal of Cell Biology, 81
R. Anderson (1977)
Biochemical and cytochemical evidence for ATPase activity in basal bodies isolated from oviductThe Journal of Cell Biology, 74
M. Kleve, W. Clark (1980)
Association of actin with sperm centrioles: isolation of centriolar complexes and immunofluorescent localization of actinThe Journal of Cell Biology, 86
We have localized a fraction of the enzyme, purine nucleoside phosphorylase (PNP), to the centrioles and basal bodies of mammalian, avian, and protozoan cells. Two completely independent methods were used, one based on the ultrastructural cytochemistry of the enzyme activity and one based on immunofluorescence microscopy using an antibody raised in rabbit against purified human PNP. PNP catalyzes the reversible conversion of purine nucleosides and inorganic phosphate to the corresponding purine bases and ribose-1-phosphate. Its partial localization to centrioles and basal bodies raises the possibility that purine compounds are involved in centriole replication and/or in the regulation of microtubule assembly in vivo. No centriolar PNP could be detected in primary skin fibroblast from two infants with severe immunodeficiency disease associated with the absence of soluble PNP. This raises the possibility that defects in centriole function may contribute to the impaired division and maturation of T lymphoid precursor in this inherited disorder. Initially, the immunofluorescence analyses were complicated by a residual centriole-binding antibody that persisted in immunoglobulins from immune animals after complete removal of anti-PNP by affinity chromatography. Binding was abolished by exposure of cells to sodium periodate, indicating that this (and possibly other) "spontaneous" anticentriole antibodies in rabbit serum may be directed against carbohydrates.
The Journal of Cell Biology – Rockefeller University Press
Published: Dec 1, 1981
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.