Access the full text.
Sign up today, get DeepDyve free for 14 days.
Department of Energy/ Environmental Protection Agency contract EPA-IAG- D5-E-681-AQ, and U . S. Public Health Service grant HD-05615
T. Kurihara, Y. Tsukada (1967)
THE REGIONAL AND SUBCELLULAR DISTRIBUTION OF 2′,3′‐CYCLIC NUCLEOTIDE 3′‐PHOSPHOHYDROLASE IN THE CENTRAL NERVOUS SYSTEMJournal of Neurochemistry, 14
(1977)
Immunocytochemica l localization of glycerol-3-phosphate dehydrogenase in rat 902 THE JOURNAL OF CELL BIOLOGY -VOLUME 85, 1980 brain: are oligodendrocytes target cells for glucocorticoids? Sac
S. Poduslo, W. Norton (1975)
Isolation of specific brain cells.Methods in enzymology, 35
O. Lowry, N. Rosebrough, A. Farr, R. Randall (1951)
Protein measurement with the Folin phenol reagent.The Journal of biological chemistry, 193 1
(1980)
Receivedfor publication 28
Joseph Prohaska, Douglas Clark, William Wells (1973)
Improved rapidity and precision in the determination of brain 2',3'-cyclic nucleotide 3'-phosphohydrolase.Analytical biochemistry, 56 1
K. McCarthy, L. Partlow (1976)
Preparation of pure neuronal and non-neuronal cultures from embryonic chick sympathetic ganglia: A new method based on both differential cell adhesiveness and the formation of homotypic neuronal aggregatesBrain Research, 114
D. Shapiro (1973)
Morphological and Biochemical Alterations in Foetal Rat Brain Cells cultured in the Presence of Monobutyryl Cyclic AMPNature, 241
S. Fedoroff, L. Hertz (1977)
Cell, tissue, and organ cultures in neurobiology.
G. Campbell, M. Schachner, S. Sharrow (1977)
Isolation of glial cell-enriched and -depleted populations from mouse cerebellum by density gradient centrifugation and electronic cell sortingBrain Research, 127
D. Morré, A. Kirksey (1978)
The effect of a deficiency of vitamin B-6 on the specific activity of 2′,3′-cyclic nucleotide 3′-phosphohydrolase of neonatal rat brainBrain Research, 146
Ken McCarthy, J. Vellis (1979)
The regulation of adenosine 3':5'-cyclic monophosphate accumulation in glia by alpha-adrenergic agonists.Life sciences, 24 7
M. Estridge, R. Bunge (1978)
Compositional analysis of growing axons from rat sympathetic neuronsThe Journal of Cell Biology, 79
Seung Kim, D. Pleasure (1978)
Tissue culture analysis of neurogenesis: Myelination and synapse formation are retarded by serum deprivationBrain Research, 145
R. Mains, P. Patterson (1973)
PRIMARY CULTURES OF DISSOCIATED SYMPATHETIC NEURONSThe Journal of Cell Biology, 59
V. Tennyson (1970)
The Fine Structure of the Nervous System.JAMA Neurology, 22
Seung Kim, D. Pleasure (1978)
Tissue culture analysis of neurogenesis. II. Lipid-free medium retards myelination in mouse spinal cord culturesBrain Research, 157
J. Matthieu, P. Honegger, B. Trapp, S. Cohen, H. Webster (1978)
Myelination in rat brain aggregating cell culturesNeuroscience, 3
B. Brinkley, Patricia Murphy, L. Carole, Richardson From (1967)
PROCEDURE FOR EMBEDDING IN SITU SELECTED CELLS CULTURED IN VITROThe Journal of Cell Biology, 35
J. Booher, M. Sensenbrenner (1972)
Growth and cultivation of dissociated neurons and glial cells from embryonic chick, rat and human brain in flask cultures.Neurobiology, 2 3
D. Dvorak, E. Gipps, C. Kidson (1978)
Isolation of specific neurones by affinity methodsNature, 271
R. Lim, S. Troy, D. Turriff (1977)
Fine structure of cultured glioblasts before and after stimulation by a glia maturation factor.Experimental cell research, 106 2
F. Mcmorris (1977)
Norepinephrine induces glial-specific enzyme activity in cultured plasma glioma cells.Proceedings of the National Academy of Sciences of the United States of America, 74 10
(1977)
Age dependent changes in neuronal and glial cell markers in cultures from rat cerebral cortex
J. Vellis, G. Brooker (1973)
Induction of Enzymes by Glucocorticoids and Catecholamines in a Rat Glial Cell Line
J. Mcginnis, J. Vellis (1978)
Glucocorticoid regulation in rat brain cell cultures. Hydrocortisone increases the rate of synthesis of glycerol phosphate dehydrogenase in C6 glioma cells.The Journal of biological chemistry, 253 23
K. McCarthy, J. Vellis (1978)
Alpah-adrenergic receptor modulation of beta-adrenergic, adenosine and prostaglandin E1 increased adenosine 3':5'-cyclic monophosphate levels in primary cultures of glia.Journal of cyclic nucleotide research, 4 1
G.A.M. Breen, J. Vellis (1974)
Regulation of glycerol phosphate dehydrogenase by hydrocortisone in dissociated rat cerebral cell cultures.Developmental biology, 41 2
A novel method has been developed for the preparation of nearly pure separate cultures of astrocytes and oligodendrocytes. The method is based on (a) the absence of viable neurons in cultures prepared from postnatal rat cerebra, (b) the stratification of astrocytes and oligodendrocytes in culture, and (c) the selective detachment of the overlying oligodendrocytes when exposed to sheer forces generated by shaking the cultures on an orbital shaker for 15--18 h at 37 degrees C. Preparations appear greater than 98% pure and contain approximately 1-2 x 10(7) viable cells (20--40 mg of cell protein). Three methods were used to characterize these two culture t ypes. First, electron microscopic examination was used to identify the cells in each preparation (mixed and separated cultures of astrocytes and oligodendrocytes) and to assess the purity of each preparation. Second, two oligodendroglial cell markers, 2',3'-cyclic nucleotide 3'-phosphohydrolase (EC 3.1.4.37) and glycerol phosphate dehydrogenase (EC 1.1.1.8) were monitored. Third, the regulation of cyclic AMP accumulation in each culture type was examined. In addition to these studies, we examined the influence of brain extract and dibutyryl cAMP on the gross morphology and ultrastructure of each preparation. These agents induced astroglial process formation without any apparent morphological effect on oligodendrocytes. Collectively, the results indicate that essentially pure cultures of astrocytes and of oligodendrocytes can be prepared and maintained. These preparations should significantly aid in efforts to examine the biochemistry, physiology, and pharmacology of these two major classes of central nervous system cells.
The Journal of Cell Biology – Rockefeller University Press
Published: Jun 1, 1980
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.