Access the full text.
Sign up today, get DeepDyve free for 14 days.
D. Roof, P. Meluh, M. Rose (1992)
Kinesin-related proteins required for assembly of the mitotic spindleThe Journal of Cell Biology, 118
Daniel Lew, Steven Reed (1992)
A proliferation of cyclins.Trends in cell biology, 2 3
T. Hunt (1991)
Destruction's our delight.Nature, 349
J. Maier (1991)
Guide to yeast genetics and molecular biology.Methods in enzymology, 194
(1994)
Large-scale analysis of gene expression, protein localization, and gene disruption in Saccharomyces cerevi~iae
T. Christianson, R. Sikorski, M. Dante, J. Shero, P. Hieter (1992)
Multifunctional yeast high-copy-number shuttle vectors.Gene, 110 1
P. Baum, C. Furlong, B. Byers (1986)
Yeast gene required for spindle pole body duplication: homology of its product with Ca2+-binding proteins.Proceedings of the National Academy of Sciences of the United States of America, 83 15
J. McGrew, L. Goetsch, B. Byers, P. Baum (1992)
Requirement for ESP1 in the nuclear division of Saccharomyces cerevisiae.Molecular biology of the cell, 3 12
L. Hartwell, David Smith (1985)
Altered fidelity of mitotic chromosome transmission in cell cycle mutants of S. cerevisiae.Genetics, 110 3
T. Navas, Zheng Zhou, S. Elledge (1995)
DNA polymerase ϵ links the DNA replication machinery to the S phase checkpointCell, 80
J. Strathern, E. Jones, J. Broach (1981)
The Molecular biology of the yeast saccharomyces, life cycle and inheritance
Mark Rose, Sue Biggins, L Satterwhite (1993)
Unravelling the tangled web at the microtubule-organizing center.Current opinion in cell biology, 5 1
I. Fitch, Christian Dahmann, U. Surana, A. Amon, K. Nasmyth, L. Goetsch, B. Byers, B. Futcher (1992)
Characterization of four B-type cyclin genes of the budding yeast Saccharomyces cerevisiae.Molecular biology of the cell, 3 7
S. Altschul, W. Gish, W. Miller, E. Myers, D. Lipman (1990)
Basic local alignment search tool.Journal of molecular biology, 215 3
V. Guacci, A. Yamamoto, A. Strunnikov, J. Kingsbury, E. Hogan, P. Meluh, D. Koshland (1993)
Structure and function of chromosomes in mitosis of budding yeast.Cold Spring Harbor symposia on quantitative biology, 58
(1988)
Isolation and cloning of conditionally lethal chromosome transmission fidelity genes in Saccharomyces cerevisiae
Estelle Lauze, B. Stoelcker, F. Luca, Eric Weiss, A. Schutz, M. Winey (1995)
Yeast spindle pole body duplication gene MPS1 encodes an essential dual specificity protein kinase.The EMBO Journal, 14
A. Amon, S. Irniger, K. Nasmyth (1994)
Closing the cell cycle circle in yeast: G2 cyclin proteolysis initiated at mitosis persists until the activation of G1 cyclins in the next cycleCell, 77
C. Holm, T. Goto, James Wang, D. Botstein (1985)
DNA topoisomerase II is required at the time of mitosis in yeastCell, 41
M. Winey, Breck Byers (1993)
Assembly and functions of the spindle pole body in budding yeast.Trends in genetics : TIG, 9 9
M. Glotzer, A. Murray, M. Kirschner (1991)
Cyclin is degraded by the ubiquitin pathwayNature, 349
M. Rout, J. Kilmartin (1990)
Components of the yeast spindle and spindle pole bodyThe Journal of Cell Biology, 111
R. McCarroll, W. Fangman (1988)
Time of replication of yeast centromeres and telomeresCell, 54
(1982)
A method for displaying the hydrophobic character of a protein
F. Sherman, G. Fink, J. Hicks (1979)
Methods in yeast genetics
(1996)
Pdslp, an inhibitor of anaphase in budding yeast, plays a critical role in the APC and check point pathway(s)
N. Grandin, Steven Reed (1993)
Differential function and expression of Saccharomyces cerevisiae B-type cyclins in mitosis and meiosisMolecular and Cellular Biology, 13
Mark Osborne, G. Schlenstedt, Timothy Jinks, Pamela Silver, Pamela Silver (1994)
Nuf2, a spindle pole body-associated protein required for nuclear division in yeastThe Journal of Cell Biology, 125
Role of Pdslp in Anaphase of Budding Yeast
M. Rose, G. Fink (1987)
KAR1, a gene required for function of both intranuclear and extranuclear microtubules in yeastCell, 48
R. Sikorski, P. Hieter (1989)
A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae.Genetics, 122 1
E. McIntosh, Tom Atkinson, R. Storms, Michael Smith (1991)
Characterization of a short, cis-acting DNA sequence which conveys cell cycle stage-dependent transcription in Saccharomyces cerevisiaeMolecular and Cellular Biology, 11
W. Saunders, M. Hoyt (1992)
Kinesin-related proteins required for structural integrity of the mitotic spindleCell, 70
J. Kilmartin, S. Dyos, D. Kershaw, J. Finch (1993)
A spacer protein in the Saccharomyces cerevisiae spindle poly body whose transcript is cell cycle-regulatedThe Journal of Cell Biology, 123
M. Winey, L. Goetsch, P. Baum, B. Byers (1991)
MPS1 and MPS2: novel yeast genes defining distinct steps of spindle pole body duplicationThe Journal of Cell Biology, 114
B. Byers, L. Goetsch (1974)
Duplication of spindle plaques and integration of the yeast cell cycle.Cold Spring Harbor symposia on quantitative biology, 38
Alexander Strunnikov, Vladimir Larionov, Douglas Koshland (1993)
SMC1: an essential yeast gene encoding a putative head-rod-tail protein is required for nuclear division and defines a new ubiquitous protein familyThe Journal of Cell Biology, 123
C Cooke, M Heck, W. Earnshaw (1987)
The inner centromere protein (INCENP) antigens: movement from inner centromere to midbody during mitosisThe Journal of Cell Biology, 105
William Saunders, Douglas Koshland, Dan Eshel, I. Gibbons, M. Andrew, Hoyt, Andrew Hoyt, Johns University (1995)
Saccharomyces cerevisiae kinesin- and dynein-related proteins required for anaphase chromosome segregationThe Journal of Cell Biology, 128
R. Golsteyn, K. Mundt, A. Fry, E. Nigg (1995)
Cell cycle regulation of the activity and subcellular localization of Plk1, a human protein kinase implicated in mitotic spindle functionThe Journal of Cell Biology, 129
A. Strunnikov, E. Hogan, D. Koshland (1995)
SMC2, a Saccharomyces cerevisiae gene essential for chromosome segregation and condensation, defines a subgroup within the SMC family.Genes & development, 9 5
Mark W, M. Hoyt, Clarence Chart, L. Goetsch, D. Botstein, Breck Byerst (1993)
NDC1: a nuclear periphery component required for yeast spindle pole body duplicationThe Journal of Cell Biology, 122
M. Hoyt, Ling He, K. Loo, William Saunders (1992)
Two Saccharomyces cerevisiae kinesin-related gene products required for mitotic spindle assemblyThe Journal of Cell Biology, 118
B. Byers (1981)
Cytology of the Yeast Life CycleCold Spring Harbor Monograph Archive, 11
J. Kilmartin, A Adams (1984)
Structural rearrangements of tubulin and actin during the cell cycle of the yeast SaccharomycesThe Journal of Cell Biology, 98
F. Sherman, G. Fink, J. Hicks (1987)
Methods in Yeast Genetics: A Laboratory Course Manual
D. Roof, P. Meluh, M. Rose (1991)
Multiple kinesin-related proteins in yeast mitosis.Cold Spring Harbor symposia on quantitative biology, 56
L. Hartwell (1974)
Saccharomyces cerevisiae cell cycle.Bacteriological reviews, 38 2
A. Spang, I. Courtney, Ursel Fackler, M. Matzner, E. Schiebel (1993)
The calcium-binding protein cell division cycle 31 of Saccharomyces cerevisiae is a component of the half bridge of the spindle pole bodyThe Journal of Cell Biology, 123
T. Yen, Gang Li, B. Schaar, Illya Szilak, D. Cleveland (1992)
CENP-E is a putative kinetochore motor that accumulates just before mitosisNature, 359
V. Guacci, E. Hogan, D. Koshland (1994)
Chromosome condensation and sister chromatid pairing in budding yeastThe Journal of Cell Biology, 125
K. Köhrer, H. Domdey (1991)
Preparation of high molecular weight RNA.Methods in enzymology, 194
L. Johnston, N. Lowndes, A. Johnson, A. Sugino (1991)
A cell-cycle-regulated trans-factor, DSC1, controls expression of DNA synthesis genes in yeast.Cold Spring Harbor symposia on quantitative biology, 56
U. Laemmli (1970)
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 227
To identify mutations that cause defects in mitosis, a collection of mutants in Saccharomyces cerevisiae was screened by a rapid visual assay for abnormal chromosome segregation. From this screen we identified one mutation, pds1-1 that was independently identified in an alternative screen for mutants that exhibit inviability after transient exposure to nocodazole and precocious disassociation of sister chromatids (Guacci, V., A. Yamamoto, A. Strunnikov, J. Kingsbury, E. Hogan, P. Meluh, and D. Koshland. 1993. CSH Symp. Quant. Biol. 58:677-685; Yamamoto, T.J., G. Li, B. Schaar, I. Szilak, and D.W. Cleveland. 1992. Nature (Lond.). 359:536-539). At 23 degrees C pds1-1 mutants exhibit frequent cell death and a 300-fold increase in chromosome loss compared to wild type. At 37 degrees C pds1-1 cells fail to elongate their spindles during anaphase. This spindle defect of pds1 mutants results from a temperature-sensitive step that occurs around the G1/S boundary about the time of spindle assembly. In the absence of spindle elongation pds1 mutants undergo cytokinesis, leading to the missegregation of both chromosomes and spindle pole bodies. After abnormal cell division pds1-1 mutants also initiate new rounds of DNA replication, spindle pole body duplication, and bud formation. Thus, in the pds1-1 mutant at 37 degrees C, cell cycle progression is uncoupled from the completion of anaphase. A pds1 deletion allele has similar phenotypes to the original allele. Taken together these results suggest that Pds1 protein plays an important role in chromosome segregation at 23 degrees C and an essential role for this process at 37 degrees C. The PDS1 gene encodes a novel 42-kD nuclear protein that has both basic and acidic domains. The level of PDS1 mRNA varies with the cell cycle with maximal accumulation around the G1/S boundary. The stability of Pds1 protein also appears to change during the cell cycle as overproduced Pds1p is stable in S and M but degraded in early G1. Therefore, expression of Pds1p is regulated apparently both transcriptionally and postranslationally during the cell cycle. The phenotypes of pds1 mutants and expression pattern of Pds1p are discussed in the context of other spindle-defective mutants and the knowledge that Pds1 protein is an inhibitor of anaphase (Yamamoto, T.J., G. Li, B. Schaar, I. Szilak, and D.W. Cleveland. 1992. Nature (Lond.). 359:536-539).
The Journal of Cell Biology – Rockefeller University Press
Published: Apr 1, 1996
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.