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INTRANUCLEAR MICROTUBULE ORGANIZING CENTER IN EARLY PROPHASE NUCLEI OF THE PLASMODIUM OF THE SLIME MOLD, PHYSARUM POLYCEPHALUM

INTRANUCLEAR MICROTUBULE ORGANIZING CENTER IN EARLY PROPHASE NUCLEI OF THE PLASMODIUM OF THE... The nuclei of the plasmodial phase of the true slime mold (myxomycetes) perform intranuclear mitosis which is not accompanied by centrioles, while those of the myxamoebae exhibit an astral type of mitosis with centrioles and nuclear envelope breakdown (2) . In fungal mitoses of centriole-lacking styles, the microtubules composing the metaphase spindle arise, in most instances, in the vicinity of variously shaped microtubule organizing centers (MTOC) (13) or spindle pole bodies (1) . These may take the form of circular or rectangular, electron-opaque, layered plaques as in yeasts and mycelial ascomycetes (14, 17), or vaguely textured spherical structures repeatedly described in basidiomycetes (5, 10, 11) . No such structure has been described in myxomycetes but recently one has been found, surprisingly, deep inside prophase nuclei of Physarum (15) . The present study provides independently made observations confirming the occurrence of this novel type of MTOC in Physarum . MATERIALS AND METHODS tored by examining small fragments of plasmodium by phase-contrast microscopy . As soon as plasmodial nuclei were seen to be entering division, pieces of plasmodium were removed at intervals of 2-4 min and fixed in 3 170 glutaraldehyde in 0.1 M cacodylate buffer at pH 7.2 containing http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Cell Biology Rockefeller University Press

INTRANUCLEAR MICROTUBULE ORGANIZING CENTER IN EARLY PROPHASE NUCLEI OF THE PLASMODIUM OF THE SLIME MOLD, PHYSARUM POLYCEPHALUM

Kenji Tanaka
The Journal of Cell Biology , Volume 57 (1): 220 – Apr 1, 1973
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Publisher
Rockefeller University Press
Copyright
Copyright © 1973 by The Rockefeller University Press
ISSN
0021-9525
eISSN
1540-8140
DOI
10.1083/jcb.57.1.220
Publisher site
See Article on Publisher Site

Abstract

The nuclei of the plasmodial phase of the true slime mold (myxomycetes) perform intranuclear mitosis which is not accompanied by centrioles, while those of the myxamoebae exhibit an astral type of mitosis with centrioles and nuclear envelope breakdown (2) . In fungal mitoses of centriole-lacking styles, the microtubules composing the metaphase spindle arise, in most instances, in the vicinity of variously shaped microtubule organizing centers (MTOC) (13) or spindle pole bodies (1) . These may take the form of circular or rectangular, electron-opaque, layered plaques as in yeasts and mycelial ascomycetes (14, 17), or vaguely textured spherical structures repeatedly described in basidiomycetes (5, 10, 11) . No such structure has been described in myxomycetes but recently one has been found, surprisingly, deep inside prophase nuclei of Physarum (15) . The present study provides independently made observations confirming the occurrence of this novel type of MTOC in Physarum . MATERIALS AND METHODS tored by examining small fragments of plasmodium by phase-contrast microscopy . As soon as plasmodial nuclei were seen to be entering division, pieces of plasmodium were removed at intervals of 2-4 min and fixed in 3 170 glutaraldehyde in 0.1 M cacodylate buffer at pH 7.2 containing

Journal

The Journal of Cell Biology – Rockefeller University Press

Published: Apr 1, 1973

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