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FLAGELLAR ELONGATION AND SHORTENING IN CHLAMYDOMONAS : II. Re-utilization of Flagellar Proteins

FLAGELLAR ELONGATION AND SHORTENING IN CHLAMYDOMONAS : II. Re-utilization of Flagellar Proteins BRIAN COYNE and JOEL L . ROSENBAUM . From the Department of Biology, Yale University, New Haven, Connecticut 06520 Regeneration of flagella amputated from the biflagellate Chlamydomonas depends in part on an available pool of flagellar precursor protein and in part on new protein synthesis (10) . Observations on the regeneration kinetics in populations and single cells of Chlamydomonas pf 16 (paralyzed flagella) revealed that when both flagella are removed (the "zero-zero" case), both regenerate to a length of 10-12 µ . In the presence of a concentration of cycloheximide adequate to inhibit amino acid incorporation into protein by at least 99% (10 µg/ml), however, regeneration is limited to about 2 .s per flagellum . From these results, it was concluded that the cells have a limited amount of the flagellar precursor protein necessary for regeneration (10) . In single cells, it was observed that if only one of the two flagella is removed (the "long-zero" case), the intact flagellum initially shortens by linear kinetics while the amputated flagellum regenerates . This simultaneous shortening and elongation continues until both flagella are of TILE JOURNAL OF CELL BIOLOGY ± VOLUME 47 . 1970 . pages 777-781 approximately equal length, http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Cell Biology Rockefeller University Press

FLAGELLAR ELONGATION AND SHORTENING IN CHLAMYDOMONAS : II. Re-utilization of Flagellar Proteins

The Journal of Cell Biology , Volume 47 (3): 777 – Dec 1, 1970

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Publisher
Rockefeller University Press
Copyright
Copyright © 1970 by The Rockefeller University Press
ISSN
0021-9525
eISSN
1540-8140
DOI
10.1083/jcb.47.3.777
Publisher site
See Article on Publisher Site

Abstract

BRIAN COYNE and JOEL L . ROSENBAUM . From the Department of Biology, Yale University, New Haven, Connecticut 06520 Regeneration of flagella amputated from the biflagellate Chlamydomonas depends in part on an available pool of flagellar precursor protein and in part on new protein synthesis (10) . Observations on the regeneration kinetics in populations and single cells of Chlamydomonas pf 16 (paralyzed flagella) revealed that when both flagella are removed (the "zero-zero" case), both regenerate to a length of 10-12 µ . In the presence of a concentration of cycloheximide adequate to inhibit amino acid incorporation into protein by at least 99% (10 µg/ml), however, regeneration is limited to about 2 .s per flagellum . From these results, it was concluded that the cells have a limited amount of the flagellar precursor protein necessary for regeneration (10) . In single cells, it was observed that if only one of the two flagella is removed (the "long-zero" case), the intact flagellum initially shortens by linear kinetics while the amputated flagellum regenerates . This simultaneous shortening and elongation continues until both flagella are of TILE JOURNAL OF CELL BIOLOGY ± VOLUME 47 . 1970 . pages 777-781 approximately equal length,

Journal

The Journal of Cell BiologyRockefeller University Press

Published: Dec 1, 1970

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