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James Thomas, D. Botstein (1986)
A gene required for the separation of chromosomes on the spindle apparatus in yeastCell, 44
N. Green (1975)
Calcium transport in contraction and secretionNature, 256
E. Vallen, Mark Hiller, T. Scherson, M. Rose, E. Vallen, T. Scherson, T. Roberts, K. Zee, T. Scher-Son, M. Rose (1992)
Separate domains of KAR1 mediate distinct functions in mitosis and nuclear fusionThe Journal of Cell Biology, 117
Christine Mirzayan, Connie Copeland, Michael Snyder (1992)
The NUF1 gene encodes an essential coiled-coil related protein that is a potential component of the yeast nucleoskeletonThe Journal of Cell Biology, 116
Bessie Huang, D. Watterson, Vincent Lee, M. Schibler (1988)
Purification and characterization of a basal body-associated Ca2+- binding proteinThe Journal of Cell Biology, 107
Vincent Lee, Bessie Huang (1993)
Molecular cloning and centrosomal localization of human caltractin.Proceedings of the National Academy of Sciences of the United States of America, 90 23
(1981)
Multiple roles of the spindle pole bodies in the life cycle
J. Kilmartin, S. Dyos, D. Kershaw, J. Finch (1993)
A spacer protein in the Saccharomyces cerevisiae spindle poly body whose transcript is cell cycle-regulatedThe Journal of Cell Biology, 123
M. Winey, L. Goetsch, P. Baum, B. Byers (1991)
MPS1 and MPS2: novel yeast genes defining distinct steps of spindle pole body duplicationThe Journal of Cell Biology, 114
B. Byers, L. Goetsch (1974)
Duplication of spindle plaques and integration of the yeast cell cycle.Cold Spring Harbor symposia on quantitative biology, 38
(1993)
Unraveling the tangled web at the microtubule-organlzing center
D. Schild, H. Ananthaswamy, R. Mortimer (1981)
An endomitotic effect of a cell cycle mutation of Saccharomyces cerevisiae.Genetics, 97 3-4
(1988)
Diverse effects of ~-tabulin mutations on microtobule formation and function
Bruce Tai, S. Adler (1992)
Mutational analysis of centrin: an EF-hand protein associated with three distinct contractile fibers in the basal body apparatus of ChlamydomonasThe Journal of Cell Biology, 119
(1989)
Vertebrate and yeast calmodulin, despite sequence divergence, are functionally interchangeable
P. Baum, C. Furlong, B. Byers (1986)
Yeast gene required for spindle pole body duplication: homology of its product with Ca2+-binding proteins.Proceedings of the National Academy of Sciences of the United States of America, 83 15
J. Salisbury, A. Baron, M. Sanders (1988)
The centrin-based cytoskeleton of Chlamydomonas reinhardtii: distribution in interphase and mitotic cellsThe Journal of Cell Biology, 107
B. Byers, L. Goetsch (1975)
Behavior of spindles and spindle plaques in the cell cycle and conjugation of Saccharomyces cerevisiaeJournal of Bacteriology, 124
C. Jacobs, A. Adams, P. Szaniszlo, J. Pringle (1988)
Functions of microtubules in the Saccharomyces cerevisiae cell cycleThe Journal of Cell Biology, 107
E. Vallen, T. Scherson, T. Roberts, K. Zee, M. Rose (1992)
Asymmetric mitotic segregation of the yeast spindle pole bodyCell, 69
Elizabeth VaUen, Warren Ho, Mark Wheyt, M. Rose (1994)
Genetic interactions between CDC31 and KAR1, two genes required for duplication of the microtubule organizing center in Saccharomyces cerevisiae.Genetics, 137 2
Christopher Lee, A. Levin, D. Branton (1987)
Copper staining: a five-minute protein stain for sodium dodecyl sulfate-polyacrylamide gels.Analytical biochemistry, 166 2
T. Davis, M. Urdea, F. Masiarz, J. Thorner (1986)
Isolation of the yeast calmodulin gene: Calmodulin is an essential proteinCell, 47
J. Geiser, H. Sundberg, Bill Chang, Eric Muller, T. Davis (1993)
The essential mitotic target of calmodulin is the 110-kilodalton component of the spindle pole body in Saccharomyces cerevisiaeMolecular and Cellular Biology, 13
A. Spang, I. Courtney, Ursel Fackler, M. Matzner, E. Schiebel (1993)
The calcium-binding protein cell division cycle 31 of Saccharomyces cerevisiae is a component of the half bridge of the spindle pole bodyThe Journal of Cell Biology, 123
(1987)
KARl, a gene required for function of both intranuclear and extranuclear microtobules in yeast
Bessie Huang, Adele Mengersen (1988)
Molecular cloning of cDNA for caltractin, a basal body-associated Ca2+- binding protein: homology in its protein sequence with calmodulin and the yeast CDC31 gene productThe Journal of Cell Biology, 107
Rex Kochanski, G. Borisy (1990)
Mode of centriole duplication and distributionThe Journal of Cell Biology, 110
M. Winey, Breck Byers (1993)
Assembly and functions of the spindle pole body in budding yeast.Trends in genetics : TIG, 9 9
M. Rout, J. Kilmartin (1990)
Components of the yeast spindle and spindle pole bodyThe Journal of Cell Biology, 111
F. Studier, B. Moffatt (1986)
Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes.Journal of molecular biology, 189 1
J. Conde, G. Fink (1976)
A mutant of Saccharomyces cerevisiae defective for nuclear fusion.Proceedings of the National Academy of Sciences of the United States of America, 73 10
F. Sherman, G. Fink, J. Hicks (1979)
Methods in yeast genetics
M. Eilers, K. Verner, S. Hwang, G. Schatz (1988)
Import of proteins into mitochondria.Philosophical transactions of the Royal Society of London. Series B, Biological sciences, 319 1193
The Saccharomyces cerevisiae genes KAR1 and CDC31 are required for the initial stages of spindle pole body (SPB) duplication in yeast. The Cdc31 protein is most related to caltractin/centrin, a calcium-binding protein present in microtubule organizing centers in many organisms. Because of a variety of genetic interactions between CDC31 and KAR1 (Vallen, E. A., W. Ho. M. Winey, and M. D. Rose. 1994. Genetics. In press), we wanted to determine whether Cdc31p and Kar1p physically interact. Cdc31p was expressed and purified from Escherichia coli and active for binding calcium. Using a protein blotting technique, Cdc31p bound to Kar1p in vitro via an essential domain in Kar1p required for SPB duplication (Vallen, E. A., M. A. Hiller, T. Y. Scherson, and M. D. Rose. 1992a. J. Cell Biol. 117:1277-1287). By immunofluorescence microscopy, we determined that the interaction also occurs in vivo. Cdc31p was localized to the SPB in wild-type cells but was mislocalized in a kar1 mutant strain. In a kar1 mutant containing a dominant CDC31 suppressor, Cdc31p was again localized to the SPB. Furthermore, the localization of Cdc31p to the SPB was affected by the overexpression of Kar1p-beta-galactosidase hybrids. Based on these data, we propose that the essential function of Kar1p is to localize Cdc31p to the SPB, and that this interaction is normally required for SPB duplication.
The Journal of Cell Biology – Rockefeller University Press
Published: May 15, 1994
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