Access the full text.
Sign up today, get DeepDyve free for 14 days.
C. Snapper, F. Finkelman, W. Paul (1988)
Differential regulation of IgG1 and IgE synthesis by interleukin 4The Journal of Experimental Medicine, 167
Human IgE synthesis in vitro is determined by the reciprocal and J . Banchereau . 1989 . Activation of human B lymphocytes antagonistic effects of 11,4 and IFN-y
G. Thyphronitis, G. Tsokos, C. June, A. Levine, F. Finkelman (1989)
IgE secretion by Epstein-Barr virus-infected purified human B lymphocytes is stimulated by interleukin 4 and suppressed by interferon gamma.Proceedings of the National Academy of Sciences of the United States of America, 86 14
T. Kinashi, N. Harada, E. Severinson, T. Tanabe, P. Sideras, M. Konishi, C. Azuma, A. Tominaga, S. Bergstedt‐Lindqvist, Masazumi Takahashi, F. Matsuda, Y. Yaoita, K. Takatsu, T. Honjo (1986)
Cloning of complementary DNA encoding T-cell replacing factor and identity with B-cell growth factor IINature, 324
(1990)
Long term proliferation of resting human B lympho-1990 . A receptor for tumor necrosis factor defines an unusual cytes through crossfnking of CD40
T. Defrance, J. Aubry, B. Vanbervliet, J. Banchereau (1986)
Human interferon-gamma acts as a B cell growth factor in the anti-IgM antibody co-stimulatory assay but has no direct B cell differentiation activity.Journal of immunology, 137 12
(1218)
J. Immunol
E. Clutterbuck, J. Shields, J. Gordon, S. Smith, A. Boyd, R. Callard, H. Campbell, I. Young, C. Sanderson (1987)
Recombinant human interleukin 5 is an eosinophil differentiation factor but has no activity in standard human B cell growth factor assaysEuropean Journal of Immunology, 17
(1986)
Amplification of the 1991. Long term human B cell lines dependent on interleukin immune response by agonistic antibodies. Immunol. Today. 4 and anti CD40
(1986)
Activation of human and expressed human Fc receptor for IgG mediates anti-CD3
D. Vercelli, H. Jabara, K. Arai, R. Geha (1989)
Induction of human IgE synthesis requires interleukin 4 and T/B cell interactions involving the T cell receptor/CD3 complex and MHC class II antigensThe Journal of Experimental Medicine, 169
M. Dawson (1991)
Cytokines and B lymphocytesImmunology Today, 12
A. Lanzavecchia (1983)
One out of five peripheral blood B lymphocytes is activated to high‐rate Ig production by human alloreactive T cell clonesEuropean Journal of Immunology, 13
J. Pène, F. Rousset, F. Brière, I. Chrétien, J. Bonnefoy, H. Spits, T. Yokota, N. Arai, K. Arai, J. Banchereau (1988)
IgE production by normal human lymphocytes is induced by interleukin 4 and suppressed by interferons gamma and alpha and prostaglandin E2.Proceedings of the National Academy of Sciences of the United States of America, 85 18
(1987)
B cell growth promoting activity of recombinant human 11x4
Received for publication 31 October 1990 and in revised form 17 December 1990
P. Parronchi, A. Tiri, D. Macchia, M. Carli, P. Biswas, C. Simonelli, E. Maggi, G. Prete, M. Ricci, S. Romagnani (1990)
Noncognate contact-dependent B cell activation can promote IL-4-dependent in vitro human IgE synthesis.Journal of immunology, 144 6
R. Coffman, J. Ohara, M. Bond, J. Carty, A. Zlotnik, W. Paul (1986)
B cell stimulatory factor-1 enhances the IgE response of lipopolysaccharide-activated B cells.Journal of immunology, 136 12
R. Peleman, G. Delespesse (1990)
In vitro synthesis of human IgE by neonatal lymphocytes: enhancing effect of interferon-gamma.Cellular immunology, 129 2
T. Defrance, B. Vanbervliet, J. Aubry, J. Banchereau (1988)
Interleukin 4 inhibits the proliferation but not the differentiation of activated human B cells in response to interleukin 2The Journal of Experimental Medicine, 168
(1987)
Recombinan t interferon -ca, -/3 and -y enhance the proliferative response of human B cells
(1987)
Effect of tumor necrosis factor a on mitogen-activated human B cells
Address correspondence to Frangoise Rousset, Laboratory for Immunological Research, Schering-Plough, 27 chemin des Peupliers
B cells mediated through two distinct cell surface differentiadependent lymphoproliferation
Human resting B lymphocytes enter a state of sustained proliferation when incubated with both mouse fibroblastic L cells stably expressing Fc gamma RII/CDw32 and anti-CD40 antibodies. We have explored the effects of 11 recombinant human cytokines (CKs) on induced cell proliferation and immunoglobulin (Ig) production. Interleukin 4 (IL-4) was the only CK able to enhance anti-CD40-induced B cell multiplication as measured by enumeration of viable cells, and interferon gamma (IFN-gamma) further stimulated this induced proliferation. IL-4 enhanced the production of IgM and IgG by B cells and induced them to produce IgE. Combinations of IL-4 and IL-2 resulted in the production of large amounts of IgM and IgA. Interestingly, IFN-gamma did not inhibit the production of IgE by cells stimulated with anti-CD40 and IL-4. None of the tested CK combinations resulted in the production of large quantities of IgG. Therefore, this new culture system represents a unique model to study isotype regulation in highly purified human B lymphocytes, in addition to allowing the generation of long-term factor-dependent human B cell lines.
The Journal of Experimental Medicine – Rockefeller University Press
Published: Mar 1, 1991
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.