Access the full text.
Sign up today, get DeepDyve free for 14 days.
R. Grand, S. Perry (1979)
Calmodulin-binding proteins from brain and other tissues.The Biochemical journal, 183 2
F. Vincenzi (1979)
Calmodulin in the regulation of intracellular calcium.Proceedings of the Western Pharmacology Society, 22
K. Sobue, K. Kanda, J. Adachi, S. Kakiuchi (1983)
Calmodulin-binding proteins that interact with actin filaments in a Ca2+-dependent flip-flop manner: survey in brain and secretory tissues.Proceedings of the National Academy of Sciences of the United States of America, 80 22
A. Somlyo (1984)
Cellular site of calcium regulationNature, 309
M. Greenberg, G. Edelman (1983)
The 34 kd pp60src substrate is located at the inner face of the plasma membraneCell, 33
J. Glenney, Mary Osborn, Klaus Weber (1982)
The intracellular localization of the microvillus 110K protein, a component considered to be involved in side-on membrane attachment of F-actin.Experimental cell research, 138 1
A. Ilundain, R. Naftalin (1979)
Role of Ca2+-dependent regulator protein in intestinal secretionNature, 279
D. Wolff, P. Poirier, C. Brostrom, M. Brostrom (1977)
Divalent cation binding properties of bovine brain Ca2+-dependent regulator protein.The Journal of biological chemistry, 252 12
E. Erikson, R. Erikson (1980)
Identification of a cellular protein substrate phosphorylated by the avian sarcoma virus-transforming gene productCell, 21
C. Howe, M. Mooseker, T. Graves (1980)
Brush-border calmodulin. A major component of the isolated microvillus coreThe Journal of Cell Biology, 85
T. Mukherjee, L. Staehelin (1971)
The fine-structural organization of the brush border of intestinal epithelial cells.Journal of cell science, 8 3
L. Eldik, D. Watterson (1981)
Reproducible production of antiserum against vertebrate calmodulin and determination of the immunoreactive site.The Journal of biological chemistry, 256 9
(1977)
Physiochemi~ properties of rat testis Ca+*-dep¢ndent regulator protein of cyclic nucleotide phosphodiesterase
J. Glenney, K. Weber (1980)
Calmodulin-binding proteins of the microfilaments present in isolated brush borders and microvilli of intestinal epithelial cells.The Journal of biological chemistry, 255 22
D. Bers (1982)
A simple method for the accurate determination of free [Ca] in Ca-EGTA solutions.The American journal of physiology, 242 5
A. Taylor, R. Wasserman (1967)
Vitamin D3-induced calcium-binding protein: partial purification, electrophoretic visualization, and tissue distribution.Archives of biochemistry and biophysics, 119 1
J. Haiech, C. Klee, J. Demaille (1981)
Effects of cations on affinity of calmodulin for calcium: ordered binding of calcium ions allows the specific activation of calmodulin-stimulated enzymes.Biochemistry, 20 13
J. Glenney, P. Glenney, K. Weber (1982)
F-actin-binding and cross-linking properties of porcine brain fodrin, a spectrin-related molecule.The Journal of biological chemistry, 257 16
J. Spudich, S. Watt (1971)
The regulation of rabbit skeletal muscle contraction. I. Biochemical studies of the interaction of the tropomyosin-troponin complex with actin and the proteolytic fragments of myosin.The Journal of biological chemistry, 246 15
P. O'Farrell, H. Goodman, P. O’Farrell (1977)
High resolution two-dimensional electrophoresis of basic as well as acidic proteinsCell, 12
(1984)
The microvillos 110 K cytoskeletal protein is an integral membrane protein
J. Harper, W. Cheung, R. Wallace, H. Huang, S. Levine, A. Steiner (1980)
Localization of calmodulin in rat tissues.Proceedings of the National Academy of Sciences of the United States of America, 77 1
C. Bishop, N. Kendrick, H. DeLuca (1983)
Induction of calcium-binding protein before 1,25-dihydroxyvitamin D3 stimulation of duodenal calcium uptake.The Journal of biological chemistry, 258 2
A. Taylor (1981)
Immunocytochemical localization of the vitamin D-induced calcium-binding protein: relocation of antigen during frozen section processing.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 29
M. Thomasset, A. Molla, Owen Parkes, Jacques Demaille (1981)
Intestinal calmodulin and calcium‐binding protein differ in their distribution and in the effect of vitamin D steroids on their concentrationFEBS Letters, 127
K. Broschat, R. Stidwill, D. Burgess (1983)
Phosphorylation controls brush border motility by regulating myosin structure and association with the cytoskeletonCell, 35
H. Huxley (1972)
7 – MOLECULAR BASIS OF CONTRACTION IN CROSS-STRIATED MUSCLES
(1984)
Comparison ofspcctrin isolated from erythroid and non-erythruid sources
N. Hirokawa, J. Heuser (1981)
Quick-freeze, deep-etch visualization of the cytoskeleton beneath surface differentiations of intestinal epithelial cellsThe Journal of Cell Biology, 91
198 I. Purification ofa calraodulinbinding protein from chicken ~77ard that interacts with F-actin
A. Bretscher, K. Weber (1978)
Localization of actin and microfilament-associated proteins in the microvilli and terminal web of the intestinal brush border by immunofluorescence microscopyThe Journal of Cell Biology, 79
(1980)
Calmodulin. Annu. Rev. Biochem
(1981)
Protein transfer to nitrocellulose filters. A simple method for quantitation of single proteins in complex mixtures. FEBS (Fed. Eur
D. Hathaway, R. Adelstein, C. Klee (1981)
Interaction of calmodulin with myosin light chain kinase and cAMP-dependent protein kinase in bovine brain.The Journal of biological chemistry, 256 15
S. Craig, L. Powell (1980)
Regulation of actin polymerization by villin, a 95,000 dalton cytoskeletal component of intestinal brush bordersCell, 22
D. Burgess, B. Prum (1982)
Reevaluation of brush border motility: calcium induces core filament solation and microvillar vesiculationThe Journal of Cell Biology, 94
S. Tsukita, H. Ishikawa, M. Kurokawa, K. Morimoto, K. Sobue, S. Kakiuchi (1983)
Binding sites of calmodulin and actin on the brain spectrin, calspectinThe Journal of Cell Biology, 97
J. Glenney, A. Bretscher, K. Weber (1980)
Calcium control of the intestinal microvillus cytoskeleton: its implications for the regulation of microfilament organizations.Proceedings of the National Academy of Sciences of the United States of America, 77 11
J. Glenney, P. Glenney, K. Weber (1982)
Erythroid spectrin, brain fodrin, and intestinal brush border proteins (TW-260/240) are related molecules containing a common calmodulin-binding subunit bound to a variant cell type-specific subunit.Proceedings of the National Academy of Sciences of the United States of America, 79 13
B. Sefton, J. Cooper, S. I., Trowbridge, E. Scolnick (1982)
Antiserum specific for the carboxy terminus of the transforming protein of Rous sarcoma virusJournal of Virology, 44
Small Intestine, Hugh NellansS, Joan Popovitch (1981)
Calmodulin-regulated, ATP-driven calcium transport by basolateral membranes of rat small intestine.The Journal of biological chemistry, 256 19
J. Harper, W. Cheung, R. Wallace, S. Levine, A. Steiner (1980)
Chapter 13 – Immunocytochemical Localization of Calmodulin in Rat Tissues
A. Bretscher, K. Weber (1980)
Villin is a major protein of the microvillus cystoskeleton which binds both G and F actin in a calcium-dependent mannerCell, 20
T. Keller, M. Mooseker (1982)
Ca++-calmodulin-dependent phosphorylation of myosin, and its role in brush border contraction in vitroThe Journal of Cell Biology, 95
R. Spencer, M. Charman, Paul Wilson, E. Lawson (1976)
Vitamin D-stimulated intestinal calcium absorption may not involve calcium-binding protein directlyNature, 263
(1983)
Ca*+-binding to calmodulin
(1982)
Analysis of cytoskeietal proteins and Ca++-dependent regulation of structure in intestinal brush borders from racbitic chicks
H. Yin, J. Hartwig, Kazuichi Maruyama, Thomas Stossel (1981)
Ca2+ control of actin filament length. Effects of macrophage gelsolin on actin polymerization.The Journal of biological chemistry, 256 18
R. Wasserman, A. Taylor (1966)
Vitamin D3-Induced Calcium-Binding Protein in Chick Intestinal MucosaScience, 152
D. Miller, G. Smith (1984)
EGTA purity and the buffering of calcium ions in physiological solutions.The American journal of physiology, 246 1 Pt 1
(1980)
Mitochondrial calcium transport: an overview
V. Gerke, K. Weber (1984)
Identity of p36K phosphorylated upon Rous sarcoma virus transformation with a protein purified from brush borders; calcium‐dependent binding to non‐erythroid spectrin and F‐actin.The EMBO Journal, 3
M. Mooseker, T. Graves, K. Wharton, N. Falco, C. Howe (1980)
Regulation of microvillus structure: calcium-dependent solation and cross-linking of actin filaments in the microvilli of intestinal epithelial cellsThe Journal of Cell Biology, 87
K. Maruyama, T. Mikawa, S. Ebashi (1984)
Detection of calcium binding proteins by 45Ca autoradiography on nitrocellulose membrane after sodium dodecyl sulfate gel electrophoresis.Journal of biochemistry, 95 2
Vincenzi Ff (1979)
Calmodulin in the regulation of intracellular calcium., 22
J. Glenney, P. Kaulfus, K. Weber (1981)
F actin assembly modulated by villin: Ca++-dependent nucleation and capping of the barbed endCell, 24
J. Glenney, P. Matsudaira, K. Weber (1982)
Chapter 11 – Calcium Control of the Intestinal Microvillus Cytoskeleton
(1980)
Purification of cbick intestinal calciumbinding protein
J. Glenney, P. Glenney, M. Osborn, K. Weber (1982)
An F-actin- and calmodulin-binding protein from isolated intestinal brush borders has a morphology related to spectrinCell, 28
O. Scharff (1979)
Comparison between measured and calculated concentrations of calcium ions in buffersAnalytica Chimica Acta, 109
(1978)
The relationship between vitamin D-stimulated calcium transport and intestinal calcium binding protein
(1981)
Calcium control of microfilaments: uncoupling of the Ca++-dependent F-actin severing activity from the F-actin bundling activity of villin by mild in vitro proteolysis
P. Matsudaira, D. Burgess (1982)
Partial reconstruction of the microvillus core bundle: characterization of villin as a Ca(++)-dependent, actin-bundling/depolymerizing proteinThe Journal of Cell Biology, 92
R. Wasserman, R. Corradino, A. Taylor (1968)
Vitamin D-dependent calcium-binding protein. Purification and some properties.The Journal of biological chemistry, 243 14
GLENNEY AND GLENNEY Ca++-regulated Events in Intestinal Brush Border
W. Cheung (1980)
Calmodulin plays a pivotal role in cellular regulation.Science, 207 4426
R. Gopalakrishna, W. Anderson (1982)
Ca2+-induced hydrophobic site on calmodulin: application for purification of calmodulin by phenyl-Sepharose affinity chromatography.Biochemical and biophysical research communications, 104 2
The intestinal epithelial cell and specifically the cytoskeleton of the brush border are thought to be controlled by micromolar levels of free calcium. Calcium-binding proteins of this system include intestinal calcium binding protein (CaBP), calmodulin (CaM), villin, and a 36,000-mol-wt protein substrate of tyrosine kinases. To assess the sequence of events as the intracellular Ca++ level rises, we determined the amount of CaM and CaBP in the intestinal epithelium by western blotting and tested the Ca++ binding of CaM and CaBP by equilibrium dialysis. The Ca++-dependent actin severing activity of villin was analyzed in the presence of physiological CaM levels and increasing calcium concentrations. In addition, we analyzed the Ca++ levels required for interaction between CaM and the microvillus 110,000-mol-wt protein as well as fodrin and the interaction between a polypeptide of 36,000 mol wt (P-36) and actin. The results suggest that CaBP serves as the predominant Ca++ buffer in the cell, but CaM can effectively buffer ionic calcium in the microvillus and thus protect actin from the severing activity of villin. CaM binds to its cytoskeletal receptors, 110,000-mol-wt protein and fodrin differently, governed by the free Ca++ and pH. The interaction between P-36 and actin, however, appears to require an unphysiologically high calcium concentration (10(-4) to 10(-3) M) to be meaningful. The results provide a coherent picture of the different Ca++ regulated events occurring when the free calcium rises into the micromolar level in this unique system. This study would suggest that as the Ca++ rises in the intestinal epithelial cell an ordered sequence of Ca++ saturation of intracellular receptors occurs with the order from the lowest to highest Ca++ requirements being CaBP less than CaM less than villin less than P-36.
The Journal of Cell Biology – Rockefeller University Press
Published: Mar 1, 1985
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.