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CILIARY MOTION IN PARAMECIUM : A Scanning Electron Microscope Study

CILIARY MOTION IN PARAMECIUM : A Scanning Electron Microscope Study P. multimicronucleatum,I grown in Vegemite medium Critical point drying was used to prepare other paramecia for the SEM . These P . multimicronucleatum2 were grown in baked lettuce medium (3) . The washing and fixation procedures were carried out as described above . Specimens were dried by the critical point method of Horridge and Tamm (1),' coated with gold-palladium, and viewed in a Cambridge Mark 2A SEM . RESULTS (3), were washed in an equilibration medium (4) containing 1 rnm CaC12, 2 mm KCI, and 5 mm Tris buffer (pH 7.0) . Cells swimming in a small volume of equilibration medium were fixed instantaneously by rapid addition of a large volume of 2 .7 6 0 Os04 and / 2 .3% HgC1 2 (modified after Parducz [5]) . After 10-15 min in fixative at room temperature, the cells were washed in distilled water . To freeze-dry specimens, small drops of cells on aluminum "spoons" were plunged into a beaker of liquid propane which floated on liquid nitrogen . Frozen specimens were quickly transferred to a liquid nitrogen-cooled cold stage in a vacuum chamber, and the chamber was pumped down . After drying was completed (overnight), the chamber was http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Cell Biology Rockefeller University Press

CILIARY MOTION IN PARAMECIUM : A Scanning Electron Microscope Study

Sidney L. Tamm
The Journal of Cell Biology , Volume 55 (1): 250 – Oct 1, 1972
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References (6)

Publisher
Rockefeller University Press
Copyright
Copyright © 1972 by The Rockefeller University Press
ISSN
0021-9525
eISSN
1540-8140
DOI
10.1083/jcb.55.1.250
Publisher site
See Article on Publisher Site

Abstract

P. multimicronucleatum,I grown in Vegemite medium Critical point drying was used to prepare other paramecia for the SEM . These P . multimicronucleatum2 were grown in baked lettuce medium (3) . The washing and fixation procedures were carried out as described above . Specimens were dried by the critical point method of Horridge and Tamm (1),' coated with gold-palladium, and viewed in a Cambridge Mark 2A SEM . RESULTS (3), were washed in an equilibration medium (4) containing 1 rnm CaC12, 2 mm KCI, and 5 mm Tris buffer (pH 7.0) . Cells swimming in a small volume of equilibration medium were fixed instantaneously by rapid addition of a large volume of 2 .7 6 0 Os04 and / 2 .3% HgC1 2 (modified after Parducz [5]) . After 10-15 min in fixative at room temperature, the cells were washed in distilled water . To freeze-dry specimens, small drops of cells on aluminum "spoons" were plunged into a beaker of liquid propane which floated on liquid nitrogen . Frozen specimens were quickly transferred to a liquid nitrogen-cooled cold stage in a vacuum chamber, and the chamber was pumped down . After drying was completed (overnight), the chamber was

Journal

The Journal of Cell Biology – Rockefeller University Press

Published: Oct 1, 1972

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