A Yeast Mutant Showing Diagnostic Markers of Early and Late Apoptosis

A Yeast Mutant Showing Diagnostic Markers of Early and Late Apoptosis A Saccharomyces cerevisiae mutant in cell division cycle gene CDC48 shows typical markers of apoptosis: membrane staining with annexin V, indicating an exposure of phosphatidylserine at the outer layer of the cytoplasmic membrane; intense staining, using the terminal deoxynucleotidyl transferase–mediated dUTP nick end labeling method, indicating DNA fragmentation; and chromatin condensation and fragmentation. The coordinate occurrence of these events at different locations in the cell, which have no obvious connection except their relation to apoptosis, implies the presence of the molecular machinery performing the basic steps of apoptosis already in yeast. Saccharomyces cerevisiae may prove a suitable model to trace the roots of apoptosis. Footnotes Address all correspondence to Kai-Uwe Fröhlich, Physiologisch-Chemisches Institut, Hoppe-Seyler-Strasse 4, Universität Tübingen, 72076 Tübingen, Germany. Tel.: 49-7071-297-3360. Fax: 49-7071-296-6390. E-mail: kaifr@uni-tuebingen.de Abbreviations used in this paper: ICE IL-1β–converting enzyme TUNEL terminal deoxynucleotidyl transferase–mediated dUTP nick end labeling Submitted: 25 April 1997 Revision received 1 August 1997 http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Cell Biology Rockefeller University Press

A Yeast Mutant Showing Diagnostic Markers of Early and Late Apoptosis

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Publisher
Rockefeller University Press
Copyright
© 1997 Rockefeller University Press
ISSN
0021-9525
eISSN
1540-8140
D.O.I.
10.1083/jcb.139.3.729
Publisher site
See Article on Publisher Site

Abstract

A Saccharomyces cerevisiae mutant in cell division cycle gene CDC48 shows typical markers of apoptosis: membrane staining with annexin V, indicating an exposure of phosphatidylserine at the outer layer of the cytoplasmic membrane; intense staining, using the terminal deoxynucleotidyl transferase–mediated dUTP nick end labeling method, indicating DNA fragmentation; and chromatin condensation and fragmentation. The coordinate occurrence of these events at different locations in the cell, which have no obvious connection except their relation to apoptosis, implies the presence of the molecular machinery performing the basic steps of apoptosis already in yeast. Saccharomyces cerevisiae may prove a suitable model to trace the roots of apoptosis. Footnotes Address all correspondence to Kai-Uwe Fröhlich, Physiologisch-Chemisches Institut, Hoppe-Seyler-Strasse 4, Universität Tübingen, 72076 Tübingen, Germany. Tel.: 49-7071-297-3360. Fax: 49-7071-296-6390. E-mail: kaifr@uni-tuebingen.de Abbreviations used in this paper: ICE IL-1β–converting enzyme TUNEL terminal deoxynucleotidyl transferase–mediated dUTP nick end labeling Submitted: 25 April 1997 Revision received 1 August 1997

Journal

The Journal of Cell BiologyRockefeller University Press

Published: Nov 3, 1997

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