A population of lymphoid cells from several animal species, including man, was identified through a membrane receptor which binds sheep red blood cells treated with antibody and complement. When cells from different lymphoid organs were incubated with EAC at 37°C, only part of the lymphocytes (named CRL) bound EAC and formed rosettes, and this interaction was shown to be C3-dependent. Mouse lymphoid cells could be specifically depleted of CRL by allowing them first to interact with EAC and then submitting the mixture to ultracentrifugation in a gradient of BSA. After ultracentrifugation, a population of cells containing 95% or more of non-CRL were recovered from the upper layers of the gradient. In addition to their different abilities to bind EAC, CRL and non-CRL from mouse lymphoid organs could be distinguished by the following properties: ( a ) CRL adhered preferentially to nylon wool at 37°C in the presence of mouse serum. ( b ) After differential flotation in a gradient of BSA, a significantly higher proportion of CRL were recovered from the upper layers of the gradient. ( c ) The population of CRL contained most of the lymphocytes bearing immunoglobulin determinants on their membranes. ( d ) The distribution of CRL was quite different among lymphocytes obtained from various lymphoid organs, and they were never found in the thymus. ( e ) The membrane receptor for EAC was not detected in plaque-forming cells of mice which had been previously immunized with burro red cells. CRL and non-CRL could not be distinguished by their life span, as they were found in similar proportions among long-lived and short-lived lymphocytes from mouse peripheral lymph nodes. The function of this receptor on the membrane of certain lymphoid cells may be related to ( a ) the trapping and localization of antigen in lymphoid organs or ( b ) the localization of lymphoid cells in inflammatory sites. Footnotes Submitted: 15 May 1970
The Journal of Experimental Medicine – Rockefeller University Press
Published: Oct 1, 1970
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