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Precision of cell density estimates and endothelial cell loss with age.

Precision of cell density estimates and endothelial cell loss with age. The precision of estimating endothelial cell density of the cornea, using a noncontact method of specular microscopy, was assessed by asking eight individuals with known densities in one operated-on and one unoperated-on eye to have photography on two occasions in the same day. Three photographs of each eye were assessed by two individuals using masking procedures. Interobserver differences were negligible and estimates were similar except for one eye with low density values. The method was applied to a natural history study in which 103 eyes had cell-density estimates at zero and two years, and mean cell loss was found to be 2%. A collateral study using only good photographs that were available at both zero and two years showed a similar loss of 1.86% and reduced the number of counts showing unexpectedly high gains and losses. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Archives of ophthalmology (Chicago, Ill. : 1960) Pubmed

Precision of cell density estimates and endothelial cell loss with age.

Archives of ophthalmology (Chicago, Ill. : 1960) , Volume 103 (10): -1396 – Nov 1, 1985

Precision of cell density estimates and endothelial cell loss with age.


Abstract

The precision of estimating endothelial cell density of the cornea, using a noncontact method of specular microscopy, was assessed by asking eight individuals with known densities in one operated-on and one unoperated-on eye to have photography on two occasions in the same day. Three photographs of each eye were assessed by two individuals using masking procedures. Interobserver differences were negligible and estimates were similar except for one eye with low density values. The method was applied to a natural history study in which 103 eyes had cell-density estimates at zero and two years, and mean cell loss was found to be 2%. A collateral study using only good photographs that were available at both zero and two years showed a similar loss of 1.86% and reduced the number of counts showing unexpectedly high gains and losses.

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/lp/pubmed/precision-of-cell-density-estimates-and-endothelial-cell-loss-with-age-oIzuvesZNN
ISSN
0003-9950
DOI
10.1001/archopht.1985.01050100054017
pmid
4051849

Abstract

The precision of estimating endothelial cell density of the cornea, using a noncontact method of specular microscopy, was assessed by asking eight individuals with known densities in one operated-on and one unoperated-on eye to have photography on two occasions in the same day. Three photographs of each eye were assessed by two individuals using masking procedures. Interobserver differences were negligible and estimates were similar except for one eye with low density values. The method was applied to a natural history study in which 103 eyes had cell-density estimates at zero and two years, and mean cell loss was found to be 2%. A collateral study using only good photographs that were available at both zero and two years showed a similar loss of 1.86% and reduced the number of counts showing unexpectedly high gains and losses.

Journal

Archives of ophthalmology (Chicago, Ill. : 1960)Pubmed

Published: Nov 1, 1985

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