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Plasmid detection and sizing in single colony lysates.

Plasmid detection and sizing in single colony lysates. A simple and contained procedure for the rapid assay of the presence and size of plasmids similar to Col El is described. Bacteria are picked from an agar plate with a toothpick, lysed with dodecyl sulfate and heat, and placed directly on an agarose gel for electrophoresis. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Science (New York, N.Y.) Pubmed

Plasmid detection and sizing in single colony lysates.

Science (New York, N.Y.) , Volume 195 (4276): -388 – Feb 24, 1977

Plasmid detection and sizing in single colony lysates.


Abstract

A simple and contained procedure for the rapid assay of the presence and size of plasmids similar to Col El is described. Bacteria are picked from an agar plate with a toothpick, lysed with dodecyl sulfate and heat, and placed directly on an agarose gel for electrophoresis.

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ISSN
0036-8075
DOI
10.1126/science.318764
pmid
318764

Abstract

A simple and contained procedure for the rapid assay of the presence and size of plasmids similar to Col El is described. Bacteria are picked from an agar plate with a toothpick, lysed with dodecyl sulfate and heat, and placed directly on an agarose gel for electrophoresis.

Journal

Science (New York, N.Y.)Pubmed

Published: Feb 24, 1977

There are no references for this article.