PLoS Neglected Tropical Diseases, Volume 13 (4) – Apr 9, 2019

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- Copyright: © 2019 Kajunguri et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Data Availability: This is a theoretical study and there are no data. Funding: The work for this paper was carried out when all of the co-authors were employed at SACEMA, which receives core funding from the Department of Science & Technology, Government of South Africa. Competing interests: The authors have declared that no competing interests exist.
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a1111111111 a1111111111 A published study used a stochastic branching process to derive equations for the mean a1111111111 and variance of the probability of, and time to, extinction in population of tsetse flies (Glos- sina spp) as a function of adult and pupal mortality, and the probabilities that a female is inseminated by a fertile male. The original derivation was partially heuristic and provided no proofs for inductive results. We provide these proofs, together with a more compact OPENACCESS way of reaching the same results. We also show that, while the published equations hold Citation: Kajunguri D, Are EB, Hargrove JW (2019) good for the case where tsetse produce male and female offspring in equal proportion, a Improved estimates for extinction probabilities and different solution is required for the more general case where the probability (β) that an off- times to extinction for populations of tsetse spring is female lies anywhere in the interval (0, 1). We confirm previous results obtained (Glossina spp). PLoS Negl Trop Dis 13(4): e0006973. https://doi.org/10.1371/journal. for the special case whereβ = 0.5 and show that extinction probability is at a minimum for pntd.0006973 β> 0.5 by an amount that increases with increasing adult female mortality. Sensitivity Editor: Oladele B. Akogun, Common Heritage analysis showed that the extinction probability was affected most by changes in adult Foundation, NIGERIA female mortality, followed by the rate of production of pupae. Because females only pro- Received: October 31, 2018 duce a single offspring approximately every 10 days, imposing a death rate of greater than about 3.5% per day will ensure the eradication of any tsetse population. These mor- Accepted: February 4, 2019 tality levels can be achieved for some species using insecticide-treated targets or cattle— Published: April 9, 2019 providing thereby a simple, effective and cost-effective method of controlling and eradicat- Copyright:© 2019 Kajunguri et al. This is an open ing tsetse, and also human and animal trypanosomiasis. Our results are of further interest access article distributed under the terms of the in the modern situation where increases in temperature are seeing the real possibility Creative Commons Attribution License, which permits unrestricted use, distribution, and that tsetse will go extinct in some areas, without the need for intervention, but have an reproduction in any medium, provided the original increased chance of surviving in other areas where they were previously unsustainable author and source are credited. due to low temperatures. Data Availability Statement: This is a theoretical study and there are no data. Funding: The work for this paper was carried out when all of the co-authors were employed at Author summary SACEMA, which receives core funding from the Department of Science & Technology, Government We derive equations for the mean and variance of the probability of, and time to, extinc- of South Africa. tion in population of tsetse flies (Glossina spp), the vectors of trypanosomiasis in sub- Competing interests: The authors have declared that no competing interests exist. PLOS Neglected Tropical Diseases | https://doi.org/10.1371/journal.pntd.0006973 April 9, 2019 1 / 15 Extinction probabilities for tsetse Saharan Africa. In so doing we provide the complete proofs for all results, which were not provided in a previously published study. We also generalise the derivation to allow for the probability that an offspring is female to lie anywhere in the interval (0, 1). The proba- bility of extinction was most sensitive to changes in adult female mortality. The unusual tsetse life cycle, with very low reproductive rates, means that populations can be eradi- cated as long as adult female mortality is raised to levels greater than about 3.5% per day. Simple bait methods of tsetse control, such as insecticide-treated targets and cattle, can therefore provide simple, affordable and effective means of eradicating tsetse populations. The results are of further interest in the modern situation where increases in temperature are seeing the real possibility that tsetse will go extinct in some areas, but have an increased chance of surviving in others where they were previously unsustainable due to low temperatures. Introduction Whereas deterministic models of the growth of populations of tsetse fly(Glossina spp). (Dip- tera: Glossinidae) are adequate for large populations [1, 2], stochastic models are more appropriate when numbers are small, particularly if the population approaches zero through natural processes and/or following attempts to eradicate the fly. At that point the focus changes from attempting to obtain deterministic predictions of future population levels, to predicting the probability that the population will go extinct, and the expected time required in order to achieve this end. Hargrove developed a stochastic model for the life history of tsetse flies (Glossina spp) and thereby provided estimates of the probability of extinction, and expected time to extinction, for these insects [3]. Such estimates were always of interest in sit- uations where there was pressure in favour of area-wide eradication of entire tsetse species [4]. The model provided estimates of the level, and duration, of control effort required to achieve eradication of a target population and could thus be valuable for financial planning of tsetse and trypanosomiasis control efforts. The formulae developed were shown to provide good estimates of the time to extinction in successful operations that had already been car- ried out. With the significant increases in temperature that have occurred over recent decades the model has assumed increased interest. It is becoming apparent that parts of Africa are becom- ing so hot that tsetse may no longer survive there. A well-documented example is the popula- tion of G. pallidipes Austen in parts of Zimbabwe. Whereas this species occurred in huge numbers in the area, for example, in the neighborhood of Rekomitjie Research Station, in the Zambezi Valley, the population has shrunk by > 99.99% over the past 30 years and now appears to be on the brink of disappearing [5, 6]. At the same time, other parts of Zimbabwe, where tsetse do not currently occur—in part because winter temperatures are too low—may soon be warm enough to support tsetse. Hwange National Park, for example, supported tsetse populations prior to the rinderpest epizootic of 1896: the fly never re-established itself in the area in the 20th Century, despite the presence of an abundance of wild hosts. In part this is due to the area always having been marginal climatically: increasing temperatures may change this balance in favour of the fly. The above considerations prompted us to revisit the original derivations, from which sev- eral things became apparent: (i) It was assumed in the original derivation that equal propor- tions of male and female offspring were produced by female tsetse. The equations presented PLOS Neglected Tropical Diseases | https://doi.org/10.1371/journal.pntd.0006973 April 9, 2019 2 / 15 Extinction probabilities for tsetse were correct for this particular case—but require modification for the more general case where the probability (β) that an offspring is female lies anywhere in the interval (0,1). (ii) At a number of points in the development it is claimed that results can be shown by induction, but the proofs are not provided. (iii) An heuristic explanation for one of the equation is mis- leading because it refers to a number > 1 as a probability. (iv) Finally, the development is restrictive in that it only treats the case where birth and death rates are constant over time. In the current paper we correct the first three problems and suggest ways of overcoming the fourth. Basic life cycle of tsetse Both sexes of tsetse (Glossina spp.) feed only on blood and are vectors of human and animal trypanosomiasis in Africa. They are also very unusual biologically. During each reproductive event, the mature adult female tsetse ovulates a single egg that is retained in the uterus until it hatches. The resulting larva develops through three instars, nourished via a milk gland, result- ing ultimately in a third instar larva that may weigh as much as, or even slightly more than, its mother. This reproductive mechanism is termed adenotrophic viviparity. The mature late- third-instar larva is typically deposited on soft soil, into which it burrows rapidly, pupating immediately and remaining underground, without feeding further, until it develops into a young adult fly. Given the large amount of energy and raw material required to produce the large pupa, the female only produces one pupa every 7–12 days: and the resulting pupa takes 3–7 weeks to develop into an adult fly—the rates for these processes depending on temperature. The teneral (i.e. unfed) adult emerging from the puparial case has the full linear dimensions of the mature adult, but has a poorly developed flight musculature, and lower levels of fat reserves than mature adults. The first 2-3 blood-meals must be used to build flight muscle and fat levels before the female can start producing her own pupae. Given the implicitly low birth rate, it is clear that tsetse populations can only survive if they are able to keep their mortality at low lev- els. In the laboratory, male and female G. m. morsitans can survive for up to 241 and 208 days, respectively [7]. In the field, the flies are seldom that long-lived and females survive for longer than males, sometimes surviving at least 130 days [8]. There is a marked loss, with increasing age, in female reproductive potential in laboratory populations, but there is little suggestion of such an effect in field flies [9]. Similarly, whereas trypanosome infection can result in increased mortality in tsetse > 50 days old, the evidence for such an effect in field flies is not as convinc- ing. For present modelling purposes we have, accordingly, ignored any effect of trypanosome infection on tsetse survival. Materials and methods In this paper, we provide full details of the derivation of the formulae used and also provide a general form of the governing equation in [3], which can accommodate all possible values ofβ. Model assumptions and development A female tsetse fly generally mates only once; it is thus crucial to include in our model the probability that a female tsetse fly is inseminated by a fertile male. We will also assume that the probability that a deposited pupa is male or female can be anywhere in the open interval (0, 1). Note that, at both endpoints, extinction occurs with probability 1.0, because the population will consist only of one gender of fly. PLOS Neglected Tropical Diseases | https://doi.org/10.1371/journal.pntd.0006973 April 9, 2019 3 / 15 Extinction probabilities for tsetse Parameters and interpretations λ daily survival probability for adult female tsetse ψ daily mortality rate for adult females = -ln(λ) φ daily survival probability for female pupae χ daily mortality rate for female pupae = -ln(φ) ν time from adult female emergence to first ovulation (days) � probability female is inseminated by a fertile male τ inter-larval period (days) P pupal duration (days) p probability female tsetse fly dies between pregnancy n and (n + 1) and produces k surviving female offspring n,k β probability deposited pupa is female https://doi.org/10.1371/journal.pntd.0006973.t001 The probability p that a female survives one pregnancy and produces one surviving female 1,1 offspring is calculated as follows: First, we know that a female tsetse fly is inseminated by a fer- (ν+τ) tile male with a probability �,then survives with probabilityλ up to the time she produces her first pupa, which itself has a probabilityβ of being female. This pupa survives the pupal P τ period with a probability φ , and the mother finally dies with a probability (1 −λ ) during the next pregnancy. Thus, combining all these factors, we obtain the probability that a female tsetse fly produces one surviving daughter after surviving one pregnancy as ðnþtÞ t p ¼ �l bφ ð1#0; l Þ: ð1Þ 1;1 In general, the probability that a female tsetse fly produces k surviving daughters, after surviv- ing n pregnancies, is given by � � n#0; k � � n 1 ðnþntÞ t n kP ð2Þ p ¼ �l ð1#0; l Þ b φ #0; φ ; n;k k b for n > 0, 1� k� n, and where are the binomial coefficients. Proof: Let A be the event ‘a mother deposits exactly n pupae’, and B be the event ‘n pupae pro- n n,k duces exactly k female adults’. We can then define nþnt t M ¼ PðA Þ ¼ �l ð1#0; l Þ: n n q ¼ PðB =A Þ: n;k n;k n It is clear that p ¼ PðA B Þ ¼ PðA Þ:PðB =A Þ ¼ M :q : ð3Þ n;k n n;k n n;k n n n;k We notice that M refers to the mother’s survival and q refers to the pupae survival. So we n n,k can base our proof by concentrating on the pupal survival since the product of the two gives the result of interest. It was actually observed that Eq (2) can be proved without resorting to induction. Notice that for each pupa there are two possibilities; either it becomes an adult female or it does not. The probability that it becomes an adult female isβφ , and the probability that it does not is then clearly (1 −βφ ). Since the probabilities are the same for all pupae, and these outcomes for different pupae are independent, the probability that there are k adult females from n PLOS Neglected Tropical Diseases | https://doi.org/10.1371/journal.pntd.0006973 April 9, 2019 4 / 15 Extinction probabilities for tsetse pupae is given by a binomial distribution as � � k n#0; k P P q ¼ ðbφ Þ ð1#0; bφ Þ n;k � � n#0; k � � n 1 k n#0; k Pk ¼ b φ b #0; φ k b � � n#0; k � � n 1 Pk P ¼ b φ #0; φ : k b Thus, from Eq (3), we obtain the expression for p as n,k p ¼ M :q n;k n n;k � � n#0; k � � n 1 ðnþntÞ t n P Pk ð4Þ ¼ �l ð1#0; l Þ b φ #0; φ : k b Note that this reduces to the governing equation in [3] whenβ = 0.5. Remarks: 1. The heuristic explanation for Eq (2) in [3] is misleading because it terms a number greater than 1 a probability. Nonetheless, the formula is correct for the case considered, and is also correct more generally with the adjustment of that term, as the proof shows. 2. The governing equation in [3] works only whenβ = 0.5. After making the correction, it can be observed that Eq (4) works for all values ofβ. Summing Eq (2) over n leads to the probability (p ) that a female tsetse fly produces k sur- viving female offspring before she dies. Thus � � 1 � � n 1 n#0; k ðnþntÞ t n kP P p ¼ �l ð1#0; l Þ b φ #0; φ Þ ð5Þ k b n¼k � � � � n 1 n t t n n#0; k kP P ¼ �lð1#0; l Þφ ðl bÞ #0; φ Þ : ð6Þ k b n¼k Evaluating the sum gives nþkt t k kP �l ð1#0; l Þb φ p ¼ k > 0: � � ��� kþ1 ð7Þ 1 P 1#0; bl #0; φ The probability that a female tsetse fly produces at least one surviving daughter before she dies can be obtained by summing Eq (7) over k > 0, to obtain nþt �l bφ p ¼ : ð8Þ ðk>0Þ t 1#0; l ð1#0; bφ ÞÞ (See S1 Text for detailed proofs of Eqs (7) and (8)). Thus, the probability that a female tsetse fly does not produce any surviving female off- spring before she dies is given by nþt �l bφ ð9Þ p ¼ 1#0; p ¼ 1#0; : 0 ðk>0Þ t 1#0; l ð1#0; bφ ÞÞ Assuming that we start with one female tsetse fly in the initial generation, which produces k PLOS Neglected Tropical Diseases | https://doi.org/10.1371/journal.pntd.0006973 April 9, 2019 5 / 15 Extinction probabilities for tsetse surviving offspring, we can write the moment generating function for the next generation as 1 1 X X k k �ðyÞ¼ p y ¼ p þ p y : k 0 k k¼0 k¼1 Substituting for p and p and putting the terms not involving k outside the summation sign 0 k we get Aþ BCð1#0; yÞ �ðyÞ ¼ ; ð10Þ Aþ Bð1#0; yÞ τ τ P ν where A = 1 −λ , B =βλ φ and C = 1 − �λ . The extinction probability can be found by solving the quadratic equation ϕ(θ) = θ, and it is the smallest non-negative root [10, 11]. Thus the extinction probability is: qffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffiffi BCþ Aþ B#0; ðBCþ Aþ BÞ #0; 4BðAþ BCÞ ð11Þ y ¼ ; 2B where B6¼ 0. This is the probability that a female tsetse population, resulting from an initial population of one adult female fly, goes to extinction. If the initial population consists of N such flies, then, assuming the independence of the probability of extinction of each female line, the prob- ability of extinction is θ . Mean and variance of female tsetse population at generation n We will use the method of moments to find the mean and variance of the expected number of offspring produced. From these variables we can then derive the mean and variance of the female tsetse population at a given generation n. th By definition, the m moment of p is given by M ¼ k p : m k k¼0 When m = 1, we obtain the first moment as nþt �l bφ ð12Þ M ¼ : 1 t ð1#0; l Þ And when m = 2, we obtain the second moment as nþt t P P �l bφ ð1#0; l ð1#0; 2bφ ÞÞ M ¼ : ð13Þ 2 2 ð1#0; l Þ (See S1 Text for the proofs of Eqs (12) and (13)). The mean, or expected number of surviving daughters of female tsetse fly is nþt �l bφ m ¼ ; ð1#0; l Þ and the variance is given by � � nþt t nþt P P P �l bφ ð1#0; l ð1#0; 2bφ ÞÞ �l bφ s ¼ #0; ; t 2 ð1#0; l Þ ð1#0; l Þ PLOS Neglected Tropical Diseases | https://doi.org/10.1371/journal.pntd.0006973 April 9, 2019 6 / 15 Extinction probabilities for tsetse where MðnÞ ¼ m : ð14Þ and ns ; m ¼ 1 VðnÞ ¼ n 2 n#0; 1 ð15Þ ð1#0; m Þs m ; m 6¼ 1: 1#0; m M(n) and V(n) are the mean and variance of the size of each generation (X ), respectively with the assumption X = 1. Eqs (14) and (15) can be shown easily by induction. Time for population of the female tsetse flies to become extinct From the general framework developed by Lange [10, 11] for the probability of extinction of a branching process. We have y ¼ p ðy Þ ; n ¼ 1; 2; 3; . . . ð16Þ n k n#0; 1 k¼0 th where θ is the probability of extinction at the n generation and k is the number of offspring. Eq (16) can be rewritten in terms of a moment generating function as �ðy Þ ¼ p ðy Þ ¼ y : ð17Þ n#0; 1 k n#0; 1 n k¼0 Thus, from (17), extinction probabilities can be calculated by starting with θ = 0, θ = ϕ(θ ), θ 0 1 0 2 = ϕ(θ ), and continuing iteratively through the generations to obtain y ¼ �ðy Þ: ð18Þ n n#0; 1 We also derived the first moments of T, based on the general formula obtained by Feller in [12] as j j EðT Þ ¼ ½ðnþ 1Þ #0; n�ð1#0; y Þ; ð19Þ n¼0 where (1-θ ) = P(T > n) and T is the extinction time. The first two moments of T are: EðTÞ ¼ ð1#0; y Þ; ð20Þ n¼0 and EðT Þ ¼ ð2nþ 1Þð1#0; y Þ: ð21Þ n¼0 Thus, using Eqs (10) and (18) and taking θ = 0, we can calculate the values of θ by iteration. 0 n The first two, for example, are: Aþ BC y ¼ �ðy Þ ¼ �ð0Þ¼ ; ð22Þ 1 0 Aþ B PLOS Neglected Tropical Diseases | https://doi.org/10.1371/journal.pntd.0006973 April 9, 2019 7 / 15 Extinction probabilities for tsetse � � Aþ BC � � Aþ BC 1 Aþ BC Aþ B � � y ¼ �ðy Þ ¼ � ¼ : ð23Þ 2 1 Aþ BC Aþ B Aþ B 1 Aþ B In a situation where there are N surviving females, with N > 1, Eqs (20) and (21) can be generalised. The probability of extinction at or before generation n is θ . If we have N surviving females, then the probability that they all become extinct at generation n is (θ ) . Thus, EðTÞ ¼ ð1#0; ðy Þ Þ; ð24Þ n¼0 and EðT Þ ¼ ð2nþ 1Þð1#0; ðy Þ Þ: ð25Þ n¼0 To estimate the mean and variance of the time to extinction for a population of N female tsetse flies, all that needs to be done is to estimate θ for a population consisting of a single fly, raise each of the values to power N, and obtain the appropriate sums. Results Extinction probabilities as a function of adult and pupal female mortality rates We produced MATLAB code to solve Eq (11) and generate the extinction probabilities for given values of parameters A, B and C. Our results were closely similar to those previously published [3], as illustrated in S1 Fig—S5 Fig in the S1 Text. For example, for a pupal duration (P) of 27 days, a time to first ovulation (ν) of 7 days, an inter-larval period (τ) of 9 days, a prob- ability ofβ = 0.5 that a deposited pupa will be female and where all females are inseminated by a fertile male (�= 1), the extinction probability for a population consisting of a single insemi- nated female fly increased linearly with adult female mortality rate (ψ), at a rate which increased with increasing pupal mortality rate (χ) (S1A Fig). If the pupal mortality is high enough, then the probability of extinction is high even if the adult mortality is low. For exam- ple ifχ = 0.03 per day, then there is a greater than 40% chance that extinction will happen, even if the adult mortality rate is only 0.01 per day. Even when there was zero pupal mortality, however, extinction was certain when adult mortality rate approached levels of 0.04 per day. When the pioneer population consisted of more than a single inseminated female, the extinc- tion probability was of course generally lower (S1B Fig). If the pupal mortality rate was even 0.005 per day, however, all populations eventually went extinct, with probability 1, as long as adult mortality rate exceeded about 0.032 per day. Extinction probabilities as a function of the probability of insemination In situations where, for example, sterile male tsetse are released into a wild population or where a population is extremely low, females may fail to mate with a fertile male and �will then fall below 1.0. When the starting population was a single inseminated female, and with other input parameters as defined above, the extinction probability decreased approximately linearly with increasing values of �(S2A Fig). Increasing the assumed value of the adult PLOS Neglected Tropical Diseases | https://doi.org/10.1371/journal.pntd.0006973 April 9, 2019 8 / 15 Extinction probabilities for tsetse mortality rate (ψ) simply shifted the whole graph of extinction probability towards a value of 1.0, without changing the rate of increase of extinction probability with �.When the pioneer population was greater than 1, the relationship with �was no longer linear (S2B Fig) and, even when the starting population was only 16 inseminated females, the extinction probability was still effectively zero when the probability of fertile insemination fell to 50%. No population could avoid extinction, however, when �was less than about 10% Extinction probabilities as a function of the probability a deposited pupa is female, and the death rate of adult females Extinction is of course certain if a population consists only of one sex, but the probability of extinction goes to 1.0 more rapidly as the probability (β), that a deposited pupa is female, goes to 0 (all male population) than as it goes to 1 (all female population, Fig 1). For adult female mortality rates very close to zero, the extinction probability goes to 1.0 asβ goes to zero: Fig 1. Extinction probability as a function of the probability a deposited pupa is female, and the adult survival probability. Input assumptions: Pioneer population N = 1 inseminated female; pupal mortality rateχ = 0:005 per day; probability female inseminated by a fertile male, �= 1.0; pupal duration, P = 27 days; time to first ovulation, ν = 7 days; inter-larval period τ = 9 days. Figures in the body of the plot show the assumed daily survival probability (λ) for adult females. https://doi.org/10.1371/journal.pntd.0006973.g001 PLOS Neglected Tropical Diseases | https://doi.org/10.1371/journal.pntd.0006973 April 9, 2019 9 / 15 Extinction probabilities for tsetse but, for higher adult death rates the limit is reached for values ofβ > 0. For example, when λ = 0.98, extinction is already certain once the female proportion among pupae drops to 30%. The minimum extinction probability always occurs for a value ofβ > 0.5, by an amount that increases as adult female mortality increases. Expected number of generations to extinction We derived the general equation for the expected number of generations to extinction for independent lines of N females in Eq (24). Eqs (22) and (23) give the first two iterations of the probability of extinction. MATLAB code was written to solve Eq (24) iteratively and thus find the expected number of generations to extinction. S3 Fig shows that the expected number of generations to extinction decreases with any increase in pupal mortality. S4(A) and S4(B) Fig show that, in the event that eradication is attempted through the release of sterile males, in order to reduce the probability that females are inseminated by fer- tile males, the eradication process will be much hastened if the mortality of the wild female population is also increased. S5 Fig gives the result of the expected number of generations to extinction against the prob- ability of insemination. From the graph, we can see that the lower the probability of insemina- tion by a fertile male, the smaller the number of generations to extinction. Discussion Our results place on a firmer footing published findings based on the restrictive assumption that a deposited pupa has an equal chance of being male or female [3]. Nonetheless, we con- firm various findings of the earlier study. For example, it is clear that tsetse populations can exist at very low population densities, and the sensitivity analysis added in the present study, also indicates the prime importance of mortality among adult females in affecting the proba- bility of extinction in tsetse populations. This result further supports the arguments adduced in the earlier paper regarding the efficacy, and the cost-efficacy, of “bait methods” of tsetse control—and we refer the reader to the earlier discussion [3]. For controlling the species of tsetse occurring in Zimbabwe—G. m. morsitans and G. pallidipes—the primacy of bait meth- ods has been well established. In a mark-recapture study carried out on Antelope Island, Lake Kariba, it was estimated that 24 odour-baited insecticide-treated targets, deployed on the 5 sq km island, killed about 2% per day of female G. m. morsitans and 8% of G. pallidipes [13, 14]. When targets were used in the Rifa Triangle, in the Zambezi Valley, at the same approximate density, populations of both species were reduced by > 99.99% and the popula- tions in the treated area only survived through invasion from adjacent untreated areas [15, 16]. Again, it was estimated that the targets were killing about 2% per day of female G.m. morsitans and up to 10% of G. pallidipes. The Rifa experiment was carried out, however, when the only odour attractants available for use with targets were acetone and 1-octen-3-ol: targets are now used with the addition of two phenols, which increase the target kill rates of G.m. morsitans by about 50%, and those of G. pallidipes by several fold [17, 18]. Moreover, the targets currently in use are nearly twice as effective as the prototypes used in the Rifa Tri- angle [19]: consequently it is estimated that odour-baited insecticide-treated targets, deployed at 4/sq km in Zimbabwe will kill at least 4% per day of female G. m. morsitans and about 10% of female G. pallidipes. As is clear from S1A Fig, these levels of imposed mortality are sufficient to ensure eradication of any population of tsetse, even if the natural, adult and pupal mortality rates are zero. These theoretical predictions are borne out for G. m. morsi- tans, which was eradicated in the Umfurudzi Safari Area using targets at the above density [20]. PLOS Neglected Tropical Diseases | https://doi.org/10.1371/journal.pntd.0006973 April 9, 2019 10 / 15 Extinction probabilities for tsetse In our modelling we do not take into account the well-established age-dependent sampling biases of stationary baits such as traps and targets [21] and the estimated imposed mortality is thus an average figure. This is appropriate, however, since the additional mortality due to tar- gets, estimated from mark-recapture data, was also a figure averaged over adult females of all ages. A suggestion of a bias that would favour targets killing larger flies finds no support in the Zimbabwe situation [22, 23]. In areas of Zimbabwe where there are cattle in tsetse areas, the use of insecticide-treated cat- tle provides an effective method that can be used in parallel with, or even instead of, insecti- cide-treated targets. The combined use of these two bait methods saw massive reductions in levels of animal trypanosomiasis in north-east Zimbabwe during the 1990s, to the point that in 1997, despite widespread monitoring of cattle, no case of animal trypanosomiasis was detected [20]. In Zimbabwe, therefore, the use of any other method in addition to odour-baited insecti- cide-treated target, and insecticide-treated cattle, appears to constitute a waste of resources. In particular the release of large numbers of laboratory-reared sterile male tsetse appears super- fluous and unjustified. In this regard the very much larger effect on the probability of extinc- tion resulting from quite modest increases in adult female mortality stands in strong contrast to the very large reduction in female fertility that must be effected in order to achieve eradica- tion (cf S1B and S2A Figs). Since the publication of the original analysis of extinction probabil- ity for tsetse in [3], there has been increased interest in using insecticide-treated targets in control operations against riverine species of tsetse, such as G. f. fuscipes and G. palpalis [24]- [31]. These species are not strongly attracted by host odour, and the kill rate per target is thus very much lower than for G. pallidipes: the riverine species can, however, be captured on much smaller targets [typically 25 × 25cm] than those required for use with savannah species [up to 2 × 1m]. It is thus economically feasible to deploy much larger numbers of these so-called “tiny targets” and use them to effect significant control of riverine tsetse species. In a trial in north- ern Uganda where tiny targets were deployed at 20 targets per linear km (giving an average density of 5.7 per sq km), it was possible to reduce the fly population by > 90%. It was noted that this reduction was more than sufficient to break the transmission cycle for Human Afri- can Trypanosomiasis in the area [30]. In the same study, experiments on islands in Lake Victo- ria, Kenya, suggested that tiny targets used at the above density were killing 6% of the female population per day. The suggestion is that a further increase in target density might result in the eradication of populations, without the need to use any ancillary methods to control tsetse or trypanosomiasis. Limitation of the study All of the results presented here have been calculated on the assumption that, for each scenario, all rates of mortality and reproduction are constant over time. In reality, in the field, tempera- tures change with time and, since tsetse are poikilotherms, all of the mortality rates and devel- opmental rates associated with reproduction also change continuously with time. The calculation of extinction probabilities is greatly complicated where temperatures are changing with time, and consideration of such situations is beyond the scope of the current study. Extreme weather events, such as prolonged spells of very hot weather, as have been experi- enced in recent years in the Zambezi Valley of Zimbabwe, may push tsetse populations close to extinction. We are currently investigating the circumstances under which it is possible to cal- culate extinction probabilities in such situations. Where we cannot obtain analytical solutions of the type derived here, when all model parameters are time-invariant, we will use simulation methods to investigate the problem. A full consideration of the issue of cost comparison PLOS Neglected Tropical Diseases | https://doi.org/10.1371/journal.pntd.0006973 April 9, 2019 11 / 15 Extinction probabilities for tsetse between control methods, and the cost savings associated with eradication versus control, is an important, but complex, issue requiring full and careful consideration that is beyond the scope of the current presentation. Our modelling is restricted to the calculation of extinction proba- bilities of populations that are closed to in and out migration. We have not attempted to extend the modelling to more complex situations where metapopulations are made up of population patches with variable inter-patch connectivity. Preliminary work suggests that extinction prob- abilities will be reduced in the latter situations [32]. Supporting information S1 Text. Proof of Eqs (7), (8), (10), (12) and (13). (PDF) S1 Fig. Extinction probability as a function of female adult mortality rates. Input assump- tions: Probability females inseminated by a fertile male, �= 1.0; probability deposited pupa is female,β = 0.5; pupal duration, P = 27 days; time to first ovulation, ν = 7 days; inter-larval period τ = 9 days. A: Extinction probability as a function of female adult, and pupal, mor- tality rates. Extra input assumption: Pioneer population N = 1 inseminated female. Figures in the body of the plot show the assumed pupal mortality rate (χ per day). (cf [3], Fig 1A). B: Extinction probability as a function of adult female mortality rate and the number of insemi- nated females in the pioneer population. Extra input assumption: Pupal mortality rate assumed constant at a level ofχ = 0.005 per day. Figures in the body of the plot show the assumed num- ber (N) of inseminated females in the pioneer population. (cf [3], Fig 1B). (TIF) S2 Fig. Extinction probability as a function of the probability that a female is inseminated by a fertile male. Input assumptions: Pupal mortality rateχ = 0.005 per day; probability depos- ited pupa is female,β = 0.5; pupal duration, P = 27 days; time to first ovulation, ν = 7 days; inter-larval period ν = 9 days. A: Extinction probability as a function of the probability that a female is inseminated by a fertile male, for different levels of adult female mortality rate. Extra input assumption: Pioneer population N = 1 inseminated female. Figures in the body of the plot show the assumed adult mortality rate (ψ per day).(cf [3], Fig 2A). B: Extinction probabil- ity as a function of the probability that a female is inseminated by a fertile male, and the num- ber of inseminated females in the pioneer population. Extra input assumption: Female adult mortality rate assumed constant at a level of ψ = 0.005 per day. Figures in the body of the plot show the numbers (N) of inseminated females in the pioneer population. (cf [3], Fig 2B). (TIF) S3 Fig. Expected number of generations to extinction as a function of pupal female mortal- ity rate, and the number of inseminated females in the pioneer population. Input assump- tions: Adult mortality rate ψ = 0.07 per day; probability deposited pupa is female,β = 0.5 probability females inseminated by a fertile male, �= 1.0; pupal duration, P = 27 days; time to first ovulation, ν = 7 days; inter-larval period τ = 9 days. Figures in the body of the plot show the number (N) of inseminated females in the pioneer population. (cf [3], Fig 5A). (TIF) S4 Fig. Expected time to extinction as a function of adult female mortality rate, the num- ber of inseminated females in the pioneer population, and the probability that a female is inseminated by a fertile male. Input assumptions: Pupal mortality rateχ = 0.005 per day; probability deposited pupa is female,β = 0.5; pupal duration, P = 27 days; time to first ovula- tion, ν = 7; inter-larval period τ = 9 days. Figures in the body of the plot show the number (N) PLOS Neglected Tropical Diseases | https://doi.org/10.1371/journal.pntd.0006973 April 9, 2019 12 / 15 Extinction probabilities for tsetse of inseminated females. (cf [3], Fig 5). A: Probability females inseminated by a fertile male, �= 1. B: Probability females inseminated by a fertile male, �= 0.1. (TIF) S5 Fig. Expected time to extinction as a function of the probability that a female is insemi- nated by a fertile male, and the number of inseminated females in the pioneer population. Input assumptions: Adult mortality rate ψ = 0.07 per day; pupal mortality rateχ = 0.005 per day; probability deposited pupa is female,β = 0.5; pupal duration, P = 27 days; time to first ovulation, ν = 7 days; inter-larval period τ = 9 days. Figures in the body of the plot show the number (N) of inseminated females (cf [3], Fig 5A). (TIF) Acknowledgments The authors are extremely grateful to Professor Ekkehard Kopp for help with putting the mathematical derivations on a sound footing. Author Contributions Conceptualization: John W. Hargrove. Formal analysis: Damian Kajunguri, Elisha B. Are, John W. Hargrove. Methodology: Elisha B. Are, John W. Hargrove. Visualization: Damian Kajunguri. Writing – original draft: Damian Kajunguri, John W. Hargrove. Writing – review & editing: Elisha B. Are, John W. Hargrove. References 1. Williams DD. Probability with martingales. Cambridge University press 1991. 2. Rogers DJ. A general model for tsetse populations. Insect Science and its Application 1990; 11: 331– 3. Hargrove JW. Extinction probabilities and times to extinction for populations of tsetse flies Glossina spp. (Diptera: Glossinidae) subjected to various control measures. Bulletin of Entomological Research 2005; 95: 1–9. https://doi.org/10.1079/BER2004335 4. Vreysen MJB, Seck MT, Sall B, Bouyer J. Tsetse flies: Their biology and control using area-wide inte- grated pest management approaches. Journal of Invertebrate Pathology 2013; 112: S15–S25. https:// doi.org/10.1016/j.jip.2012.07.026 PMID: 22878217 5. Vale GA, Hargrove JW. Predicting the effect of climate change on the abundance and distribution of tsetse flies. SACEMA Quarterly 2015 October: www.sacemaquarterly.com 6. Lord JS, Hargrove JW, Torr SJ, Vale GA. Recent climate change can explain collapsing tsetse popula- tions in Zimbabwe’s Zambezi Valley: implications for human and animal trypanosomiasis in Africa. PLoS Medicine 2018; 15(10): e1002675. 7. Chigusa Y, Ohshita M, Taya J, Kirinoki M, Yokoi H, Satoru K, Matsuda H. Relationship between longev- ity and body weight in Glossina morsitans morsitans. The Japan Society of Medical Entomology and Zoology 1997; 48: 91–96 https://doi.org/10.7601/mez.48.91 8. Hargrove JW. Age-dependent changes in the probabilities of survival and capture of the tsetse fly Glos- sina morsitans morsitans Westwood. Insect Science and its Application 1990; 11: 323–330. 9. Hargrove JW. Reproductive abnormalities in field tsetse flies in Zimbabwe. Entomologia Experimentalis et Applicata 1999; 92: 89–99. https://doi.org/10.1046/j.1570-7458.1999.00528.x 10. Lange K. Applied probability. New York, Springer-Verlag 2003. 11. Lange K, Boehnke M, Carson R. Moment computation for subcritical branching processes. Journal of Applied Probability 1981; 18: 54–64. https://doi.org/10.2307/3213166 PLOS Neglected Tropical Diseases | https://doi.org/10.1371/journal.pntd.0006973 April 9, 2019 13 / 15 Extinction probabilities for tsetse 12. Feller W. An introduction to probability theory and its applications. Jonh Willey, New York 1971; 2: 3. 13. Vale GA, Hargrove JW, Cockbill GF, Phelps RJ. Field trials of baits to control populations of Glossina morsitans morsitans Westwood and G. pallidipes Austen (Diptera: Glossinidae). Bulletin of Entomologi- cal Research 1986; 76: 179–193. https://doi.org/10.1017/S000748530001467X 14. Hargrove JW. & Williams BG. Optimized simulation as an aid to modelling, with an application to the study of a population of tsetse flies, Glossina morsitans morsitans Westwood (Diptera: Glossinidae). Bulletin of Entomological Research 1998; 88, 425–435. https://doi.org/10.1017/ S0007485300042164 15. Vale GA., Lovemore DF, Flint S, Cockbill GF. Odour-baited targets to control tsetse flies, Glossina spp. (Diptera: Glossinidae), in Zimbabwe. Bulletin of Entomological Research 1988; 78: 31–49. https://doi. org/10.1017/S0007485300016059 16. Hargrove JW. Optimized simulation of the control of tsetse flies Glossina pallidipes and G. m. morsitans using odour-baited targets in Zimbabwe. Bulletin of Entomological Research 2003; 93: 19–29. https:// doi.org/10.1079/BER2002205 PMID: 12593679 17. Vale GA, Hall DR, Gough AJE. The olfactory responses of tsetse flies, Glossina spp. (Diptera: Glossini- dae), to phenols and urine in the field. Bulletin of Entomological Research 1988; 78: 293–300. https:// doi.org/10.1017/S0007485300016059 18. Bursell E, Gough AJE, Beevor PS, Cork A, Hall DR, Vale GA. Identification of components of cattle urine attractive to tsetse flies, Glossina (Diptera: Glossinidae). Bulletin of Entomological Research 1988; 78: 293–300. 19. Vale GA. Development of baits for tsetse flies (Diptera: Glossinidae) in Zimbabwe. Journal of Medical Entomology 1993; 30: 831–842. https://doi.org/10.1093/jmedent/30.5.831 PMID: 8254629 20. Hargrove JW. Tsetse eradication; sufficiency, necessity and desirability. DFID Animal Health Pro- gramme, Edinburgh, UK. 2003; 133 + ix pp 21. Hargrove JW. Ovarian ages of tsetse flies (Diptera: Glossinidae) caught from mobile and stationary baits in the presence and absence of humans. Bulletin of Entomological Research 1991; 81: 43–50. https://doi.org/10.1017/S0007485300053220 22. Hargrove JW., English S, Torr SJ, Lord J, Haines LR, van Schalkwyk C, James Patterson J, Vale G. Wing length and host location in tsetse (Glossina spp): implications for control using stationary baits. Parasites and Vectors 2019; 12:24 https://doi.org/10.1186/s13071-018-3274-x PMID: 23. Mbewe NJ, Saini RK, Torto B, Irungua J, Yusuf AA, Pirk C Effects of vector control on the population structure of tsetse (Glossina fuscipes fuscipes) in western Kenya. Acta Tropica 2018; 179: 1–9. https:// doi.org/10.1016/j.actatropica.2017.12.015 PMID: 29248414 24. Lindh JM, Torr SJ, Vale GA, Lehane MJ. Improving the cost-effectiveness of artificial visual baits for controlling the tsetse fly Glossina fuscipes fuscipes. PLoS Neglected Tropical Diseases 2009; 3(7): e474. https://doi.org/10.1371/journal.pntd.0000474 PMID: 19582138 25. Esterhuizen J, Rayaisse JB, Tirados I, Mpiana S, Solano P, Vale GA, Lehane MJ, Torr SJ Improving the cost-effectiveness of visual devices for the control of riverine tsetse flies, the major vectors of human African trypanosomiasis. PLoS Neglected Tropical Diseases 2011; 5(8): e1257. https://doi.org/ 10.1371/journal.pntd.0001257 PMID: 21829743 26. Rayaisse JB, Esterhuizen J, Tirados I, Kaba D, Salou E, et al. Towards an optimal design of target for tsetse control: comparisons of novel targets for the control of palpalis group tsetse in West Africa. PLoS Neglected Tropical Diseases 2011; 5(9): e1332. https://doi.org/10.1371/journal.pntd.0001332 PMID: 27. Lindh JM, Goswami P, Blackburn RS, Arnold SEJ, Vale GA, Lehane MJ, Torr SJ. Optimizing the colour and fabric of targets for the control of the tsetse fly Glossina fuscipes fuscipes. PLoS Neglected Tropical Diseases 2012; 6(5): e1661. https://doi.org/10.1371/journal.pntd.0001661 PMID: 22666511 28. Solano P, Torr SJ, Lehane MJ. Is vector control needed to eliminate gambiense human African trypano- somiasis? Frontiers in Cellular and Infection Microbiology 2013; 1–3. 29. Shaw APM, Tirados I, Mangwiro CTN, Esterhuizen J, Lehane MJ, Torr SJ, et al. Costs of using “tiny tar- gets” to control Glossina fuscipes fuscipes, a vector of gambiense sleeping sickness in Arua district of Uganda. PLoS Neglected Tropical Diseases 2015; 9(3): e0003624. https://doi.org/10.1371/journal. pntd.0003624 PMID: 25811956 30. Tirados I, Esterhuizen J, Kovacic V, Mangwiro TNC, Vale GA, Hastings I, et al. Tsetse control and Gam- bian sleeping sickness; implications for control strategy. PLoS Neglected Tropical Diseases 2015; 9(8): e0003822. https://doi.org/10.1371/journal.pntd.0003822 PMID: 26267814 31. Lehane M, Alfaroukh I, Bucheton B, Camara M, Harris A, Kaba D, Lumbala C, Peka M, Rayaisse JB, Waiswa C, Solano P, Torr S. Tsetse control and the elimination of Gambian sleeping sickness. PLoS PLOS Neglected Tropical Diseases | https://doi.org/10.1371/journal.pntd.0006973 April 9, 2019 14 / 15 Extinction probabilities for tsetse Neglected Tropical Diseases 2016; 10(4): e0004437. https://doi.org/10.1371/journal.pntd.0004437 PMID: 27128795 32. Peck SL. Networks of habitat patches in tsetse fly control: Implications of metapopulation structure on assessing local extinction probabilities. Ecological Modelling 2012; 246: 99–102. https://doi.org/10. 1016/j.ecolmodel.2012.07.016 PLOS Neglected Tropical Diseases | https://doi.org/10.1371/journal.pntd.0006973 April 9, 2019 15 / 15

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