Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Transformation of immortalized woodchuck hepatic cell lines with the c-Ha-ras proto-oncogene

Transformation of immortalized woodchuck hepatic cell lines with the c-Ha-ras proto-oncogene Woodchuck hepatocytes were immortalized with the simian virus 40 T antigen (SV40T-ag) oncogene and utilized in an oncogenic transformation assay. Transfectionof these cell lines with an activated c-Ha-ras oncogene (EJ6.6)resulted in the transformation of cells to a phenotype characterized byanchorage-independent growth in soft agar. Colonies of transformed cells weresubcloned and up to 80% were positive for oncoprotein expression detectedby immunoblotand Northern blot procedures. When compared with the parental celllines, ras-transformed derivatives were altered bothmorphologically and in growth rate. The tumorigenic potential ofc-Ha-ras transformed cells was demonstrated in severecombined immunodeficient (SCID) mice. There was a latency period of 1 to 4 weeksbefore tumors were detectable and a period of over 7 weeks was required fortumors to reach a diameter of 1 cm. Histologically, tumorsderived from celllines fully transformed by the SV40 T-ag had the appearance of welldifferentiated hepatocellular carcinoma (HCC) while tumors derived fromc-Ha-ras transformed cell lines had the appearance ofpoorly differentiated HCC. The capacity to induce oncogenic transformationevents in immortalized woodchuck hepatic cell lines should provide theopportunity to study the cooperative effects of hepadnaviral genes inhepatocarcinogenesis in vitro. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Carcinogenesis Oxford University Press

Transformation of immortalized woodchuck hepatic cell lines with the c-Ha-ras proto-oncogene

Jacob, James R.; Tennant, Bud C.
Carcinogenesis , Volume 17 (4) – Apr 1, 1996
Download PDF
6 pages

Loading next page...
 
/lp/oxford-university-press/transformation-of-immortalized-woodchuck-hepatic-cell-lines-with-the-c-0eX0VblZnP

References (29)

Publisher
Oxford University Press
Copyright
© Oxford University Press
ISSN
0143-3334
eISSN
1460-2180
DOI
10.1093/carcin/17.4.631
Publisher site
See Article on Publisher Site

Abstract

Woodchuck hepatocytes were immortalized with the simian virus 40 T antigen (SV40T-ag) oncogene and utilized in an oncogenic transformation assay. Transfectionof these cell lines with an activated c-Ha-ras oncogene (EJ6.6)resulted in the transformation of cells to a phenotype characterized byanchorage-independent growth in soft agar. Colonies of transformed cells weresubcloned and up to 80% were positive for oncoprotein expression detectedby immunoblotand Northern blot procedures. When compared with the parental celllines, ras-transformed derivatives were altered bothmorphologically and in growth rate. The tumorigenic potential ofc-Ha-ras transformed cells was demonstrated in severecombined immunodeficient (SCID) mice. There was a latency period of 1 to 4 weeksbefore tumors were detectable and a period of over 7 weeks was required fortumors to reach a diameter of 1 cm. Histologically, tumorsderived from celllines fully transformed by the SV40 T-ag had the appearance of welldifferentiated hepatocellular carcinoma (HCC) while tumors derived fromc-Ha-ras transformed cell lines had the appearance ofpoorly differentiated HCC. The capacity to induce oncogenic transformationevents in immortalized woodchuck hepatic cell lines should provide theopportunity to study the cooperative effects of hepadnaviral genes inhepatocarcinogenesis in vitro.

Journal

Carcinogenesis – Oxford University Press

Published: Apr 1, 1996

There are no references for this article.