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Transcriptional regulation of human β-galactoside α2,6-sialyltransferase (hST6Gal I) gene during differentiation of the HL-60 cell line

Transcriptional regulation of human β-galactoside α2,6-sialyltransferase (hST6Gal I) gene during... We have previously shown that the expression of β-galactoside α2,6-sialyltransferase (hST6Gal I) mRNA decreases during HL-60 differentiation induced with dimethyl sulfoxide (DMSO) and that transcriptional regulation depends on the P3 promoter that exists 5′-upstream of exon Y (A.Taniguchi et al. , FEBS Lett. , 441 , 191–194, 1998). The regulation of hST6Gal I may be important for the expression of sialyl-Le x in HL-60 cells. In the present report, we studied the transcriptional regulation of hST6Gal I gene during DMSO-induced differentiation of HL-60 cells. To elucidate the molecular basis of hST6Gal I gene expression, the genomic region containing the P3 promoter of hST6Gal I was isolated and functionally characterized. Using a luciferase assay, we identified a functional DNA portion that confers an enhancer, located at nucleotide number (nt) –317 to –174 within the P3 promoter of hST6Gal I genomic DNA. This element contains two sequences similar to Sp1 (GC-box) and one sequence similar to Oct-1 recognition motifs (octamer sequence). Site-directed mutagenesis of Sp1 and Oct-1 sites showed that two Sp1 motifs and one Oct-1 motif are essential for transcriptional activity in HL-60 cells. Enhancer activity is suppressed during HL-60 cell differentiation induced with DMSO. These results suggest that GC-box and octamer sequence may play a critical role in the transcriptional regulation of the hST6Gal I gene during HL-60 cell differentiation. Key words http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Glycobiology Oxford University Press

Transcriptional regulation of human β-galactoside α2,6-sialyltransferase (hST6Gal I) gene during differentiation of the HL-60 cell line

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References (50)

Publisher
Oxford University Press
Copyright
Copyright © 2015 Oxford University Press
ISSN
0959-6658
eISSN
1460-2423
DOI
10.1093/glycob/10.6.623
Publisher site
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Abstract

We have previously shown that the expression of β-galactoside α2,6-sialyltransferase (hST6Gal I) mRNA decreases during HL-60 differentiation induced with dimethyl sulfoxide (DMSO) and that transcriptional regulation depends on the P3 promoter that exists 5′-upstream of exon Y (A.Taniguchi et al. , FEBS Lett. , 441 , 191–194, 1998). The regulation of hST6Gal I may be important for the expression of sialyl-Le x in HL-60 cells. In the present report, we studied the transcriptional regulation of hST6Gal I gene during DMSO-induced differentiation of HL-60 cells. To elucidate the molecular basis of hST6Gal I gene expression, the genomic region containing the P3 promoter of hST6Gal I was isolated and functionally characterized. Using a luciferase assay, we identified a functional DNA portion that confers an enhancer, located at nucleotide number (nt) –317 to –174 within the P3 promoter of hST6Gal I genomic DNA. This element contains two sequences similar to Sp1 (GC-box) and one sequence similar to Oct-1 recognition motifs (octamer sequence). Site-directed mutagenesis of Sp1 and Oct-1 sites showed that two Sp1 motifs and one Oct-1 motif are essential for transcriptional activity in HL-60 cells. Enhancer activity is suppressed during HL-60 cell differentiation induced with DMSO. These results suggest that GC-box and octamer sequence may play a critical role in the transcriptional regulation of the hST6Gal I gene during HL-60 cell differentiation. Key words

Journal

Glycobiology – Oxford University Press

Published: Jun 1, 2000

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