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TOXICITY OF GWN-9845 AND GWN-10054 BY INGESTION AND CONTACT TO 3RD INSTAR DIAMONDBACK MOTH, 2011

TOXICITY OF GWN-9845 AND GWN-10054 BY INGESTION AND CONTACT TO 3RD INSTAR DIAMONDBACK MOTH, 2011 Arthropod Management Tests 2012, Vol. 37 doi: 10.4182/amt.2012.L2 (L2) COLLARD: Brassica oleracea L. (Acephala group), ‘Georgia Southern’ RD TOXICITY OF GWN-9845 AND GWN-10054 BY INGESTION AND CONTACT TO 3 INSTAR DIAMONDBACK MOTH, 2011 Gary. L. Leibee Mid-Florida REC, IFAS, University of Florida 2725 Binion Road Apopka, Florida 32703-8504 Phone: (407) 884-2034 Ext. 154 Fax: (407) 814-6186 E-mail: glleibee@ufl.edu Moh Leng Kok-Yokomi E-mail: mlkok@ufl.edu Luis F. Aristizabal E-mail: larist@ufl.edu Steven P. Arthurs E-mail: spa@ufl.edu Diamondback moth: Plutella xylostella (Linnaeus) rd The ingestion and contact toxicity of GWN-9845 and GWN-10054 to 3 Instar DBM were determined using a leaf dip and contact bioassay, respectively. For ingestion toxicity, 6.5-cm dia. leaf disks were cut from collard leaves. Each disk was dipped for five s and air dried. MSO (Loveland Industries Inc., Greeley, CO) was used at the rate of 0.5 % (v/v) as a wetting agent. The untreated check was dipped in water plus wetting agent. Plastic Petri dishes (100 X 15 mm, Fisherbrand, Cat. # 8-757-12) were used for bioassay units. The bioassay unit was assembled by placing 11-cm dia. filter paper on the upside down Petri dish lid, placing treated disk on filter paper and pressing Petri dish bottom into lid. Filter paper was bigger than lids and served to create a snug fit between lid and bottom rd to prevent larvae from escaping. Filter paper also absorbed excessive moisture released from damaged leaf disk. About 10 3 instars were placed on each disk before final assembly of bioassay unit. The assembled bioassay units were placed in a plastic sweater box with lid with a moist paper towel and maintained at 25ºC and 15:9 photoperiod: scotoperiod. Mortality was determined after 48 h. rd Moribund larvae were counted as dead. For contact toxicity, 3 instars of DBM were bioassayed as in the ingestion study, but the larvae were treated first and then placed on untreated collard leaf disks in the bioassay unit. Larvae were treated by spraying with serial concentrations of the insecticides using a Potter Tower (Burkard Scientific Ltd., Uxbridge, Mddx., UK). The insecticides were applied in 2-ml of spray over a three s period to about 10 larvae placed in a Petri dish lined with 9-cm dia. P8 Fisherbrand filter paper. After treatment, the dish with the larvae was transferred to a fume hood to allow the filter paper and larvae to dry before transferring the larvae to the bioassay units. Concentration-response data were analyzed using the computer software program PoloPlus (LeOra Software, Petaluma CA). The estimated LC for each bioassay type is listed in Table 1. Based on LC , GWN-9845 was 510-fold more toxic than GWN-10054 50, 90 50 by ingestion and 116-fold more toxic than GWN-10054 by contact. Based on LC , GWN-9845 was 148-fold more toxic by ingestion than by contact and GWN-10054 was 34-fold more toxic by ingestion than by contact. Table 1. Toxicity of GWN-9845 and GWN-10054 by ingestion and contact to 3rd instar DBM. 1 1 Method Insecticide Slope±SE LC (95% CI) LC (95% CI) 50 90 Ingestion GWN-9845 3.599±0.912 0.015 (0.010-0.020) 0.034 (0.024-0.075) GWN-10054 5.485±1.530 7.645 (5.792-10.378) 13.093 (9.815-28.417) Contact GWN-9845 1.997±0.569 2.220 (1.116-3.119) 9.733 (6.012-42.545) GWN-10054 2.549±0.842 258.270 (158.122-372.492) 822.065 (502.575-6328.237) ppm AI, CI = Confidence Interval http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Arthropod Management Tests Oxford University Press

TOXICITY OF GWN-9845 AND GWN-10054 BY INGESTION AND CONTACT TO 3RD INSTAR DIAMONDBACK MOTH, 2011

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Oxford University Press
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© Published by Oxford University Press.
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10.4182/amt.2012.L2
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Abstract

Arthropod Management Tests 2012, Vol. 37 doi: 10.4182/amt.2012.L2 (L2) COLLARD: Brassica oleracea L. (Acephala group), ‘Georgia Southern’ RD TOXICITY OF GWN-9845 AND GWN-10054 BY INGESTION AND CONTACT TO 3 INSTAR DIAMONDBACK MOTH, 2011 Gary. L. Leibee Mid-Florida REC, IFAS, University of Florida 2725 Binion Road Apopka, Florida 32703-8504 Phone: (407) 884-2034 Ext. 154 Fax: (407) 814-6186 E-mail: glleibee@ufl.edu Moh Leng Kok-Yokomi E-mail: mlkok@ufl.edu Luis F. Aristizabal E-mail: larist@ufl.edu Steven P. Arthurs E-mail: spa@ufl.edu Diamondback moth: Plutella xylostella (Linnaeus) rd The ingestion and contact toxicity of GWN-9845 and GWN-10054 to 3 Instar DBM were determined using a leaf dip and contact bioassay, respectively. For ingestion toxicity, 6.5-cm dia. leaf disks were cut from collard leaves. Each disk was dipped for five s and air dried. MSO (Loveland Industries Inc., Greeley, CO) was used at the rate of 0.5 % (v/v) as a wetting agent. The untreated check was dipped in water plus wetting agent. Plastic Petri dishes (100 X 15 mm, Fisherbrand, Cat. # 8-757-12) were used for bioassay units. The bioassay unit was assembled by placing 11-cm dia. filter paper on the upside down Petri dish lid, placing treated disk on filter paper and pressing Petri dish bottom into lid. Filter paper was bigger than lids and served to create a snug fit between lid and bottom rd to prevent larvae from escaping. Filter paper also absorbed excessive moisture released from damaged leaf disk. About 10 3 instars were placed on each disk before final assembly of bioassay unit. The assembled bioassay units were placed in a plastic sweater box with lid with a moist paper towel and maintained at 25ºC and 15:9 photoperiod: scotoperiod. Mortality was determined after 48 h. rd Moribund larvae were counted as dead. For contact toxicity, 3 instars of DBM were bioassayed as in the ingestion study, but the larvae were treated first and then placed on untreated collard leaf disks in the bioassay unit. Larvae were treated by spraying with serial concentrations of the insecticides using a Potter Tower (Burkard Scientific Ltd., Uxbridge, Mddx., UK). The insecticides were applied in 2-ml of spray over a three s period to about 10 larvae placed in a Petri dish lined with 9-cm dia. P8 Fisherbrand filter paper. After treatment, the dish with the larvae was transferred to a fume hood to allow the filter paper and larvae to dry before transferring the larvae to the bioassay units. Concentration-response data were analyzed using the computer software program PoloPlus (LeOra Software, Petaluma CA). The estimated LC for each bioassay type is listed in Table 1. Based on LC , GWN-9845 was 510-fold more toxic than GWN-10054 50, 90 50 by ingestion and 116-fold more toxic than GWN-10054 by contact. Based on LC , GWN-9845 was 148-fold more toxic by ingestion than by contact and GWN-10054 was 34-fold more toxic by ingestion than by contact. Table 1. Toxicity of GWN-9845 and GWN-10054 by ingestion and contact to 3rd instar DBM. 1 1 Method Insecticide Slope±SE LC (95% CI) LC (95% CI) 50 90 Ingestion GWN-9845 3.599±0.912 0.015 (0.010-0.020) 0.034 (0.024-0.075) GWN-10054 5.485±1.530 7.645 (5.792-10.378) 13.093 (9.815-28.417) Contact GWN-9845 1.997±0.569 2.220 (1.116-3.119) 9.733 (6.012-42.545) GWN-10054 2.549±0.842 258.270 (158.122-372.492) 822.065 (502.575-6328.237) ppm AI, CI = Confidence Interval

Journal

Arthropod Management TestsOxford University Press

Published: Jan 1, 2012

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