Access the full text.
Sign up today, get DeepDyve free for 14 days.
H. Morris, A. Dell, R. Easton, M. Panico, H. Koistinen, R. Koistinen, S. Oehninger, M. Patankar, M. Seppala, G. Clark (1996)
Gender-specific Glycosylation of Human Glycodelin Affects Its Contraceptive Activity*The Journal of Biological Chemistry, 271
C. Radcliffe, G. Diedrich, D. Harvey, R. Dwek, P. Cresswell, P. Rudd (2002)
Identification of Specific Glycoforms of Major Histocompatibility Complex Class I Heavy Chains Suggests That Class I Peptide Loading Is an Adaptation of the Quality Control Pathway Involving Calreticulin and ERp57*The Journal of Biological Chemistry, 277
A. Hooker, Nicola Green, A. Baines, A. Bull, N. Jenkins, P. Strange, P. Strange, D. James (1999)
Constraints on the transport and glycosylation of recombinant IFN-gamma in Chinese hamster ovary and insect cells.Biotechnology and bioengineering, 63 5
J. Bigge, T. Patel, J. Bruce, Paul Goulding, S. Charles, R. Parekh (1995)
Nonselective and efficient fluorescent labeling of glycans using 2-amino benzamide and anthranilic acid.Analytical biochemistry, 230 2
Andreas Steiner, A. Stütz (2004)
Simple syntheses of 4-O-glucosylated 1-deoxynojirimycins from maltose and cellobiose.Carbohydrate research, 339 15
L. Royle, T. Mattu, Edmund Hart, J. Langridge, A. Merry, Neil Murphy, D. Harvey, R. Dwek, P. Rudd (2002)
An analytical and structural database provides a strategy for sequencing O-glycans from microgram quantities of glycoproteins.Analytical biochemistry, 304 1
G. Fabini, A. Freilinger, F. Altmann, I. Wilson (2001)
Identification of core alpha 1,3-fucosylated glycans and cloning of the requisite fucosyltransferase cDNA from Drosophila melanogaster. Potential basis of the neural anti-horseadish peroxidase epitope.The Journal of biological chemistry, 276 30
D. Harvey (2005)
Fragmentation of negative ions from carbohydrates: Part 3. Fragmentation of hybrid and complex N-linked glycansJournal of the American Society for Mass Spectrometry, 16
A. Petrescu, A. Milac, S. Petrescu, R. Dwek, M. Wormald (2003)
Statistical analysis of the protein environment of N-glycosylation sites: implications for occupancy, structure, and folding.Glycobiology, 14 2
Yunping Huang, Y. Mechref, M. Novotny (2001)
Microscale nonreductive release of O-linked glycans for subsequent analysis through MALDI mass spectrometry and capillary electrophoresis.Analytical chemistry, 73 24
Donald Davidson, Malcolm Fraser, Francis Castellino (1990)
Oligosaccharide processing in the expression of human plasminogen cDNA by lepidopteran insect (Spodoptera frugiperda) cells.Biochemistry, 29 23
P. Kraulis (1991)
A program to produce both detailed and schematic plots of protein structures
T. Kost, J. Condreay, D. Jarvis (2005)
Baculovirus as versatile vectors for protein expression in insect and mammalian cellsNature Biotechnology, 23
E. Goldwasser, C. Kung, J. Eliason (1974)
On the mechanism of erythropoietin-induced differentiation. 13. The role of sialic acid in erythropoietin action.The Journal of biological chemistry, 249 13
M. Seppälä, H. Koistinen, R. Koistinen, A. Dell, H. Morris, S. Oehninger, G. Clark (1997)
Glycodelins as regulators of early events of reproductionClinical Endocrinology, 46
G. Guile, P. Rudd, D. Wing, S. Prime, R. Dwek (1996)
A rapid high-resolution high-performance liquid chromatographic method for separating glycan mixtures and analyzing oligosaccharide profiles.Analytical biochemistry, 240 2
S. Karri, D. Mukhopadhyay, L. Jing, A. Karande (2000)
Characterization of Monoclonal Antibodies to Glycodelin and Recombinant GlycodelinThe Histochemical Journal, 32
M. Wormald, A. Petrescu, Y. Pao, A. Glithero, Tim Elliott, R. Dwek (2002)
Conformational studies of oligosaccharides and glycopeptides: complementarity of NMR, X-ray crystallography, and molecular modelling.Chemical reviews, 102 2
M. Seppälä (2004)
Advances in uterine protein research: reproduction and cancerInternational Journal of Gynecology & Obstetrics, 85
AE Bolton, A. Pockley, KJ Clough (1987)
IDENTIFICATION OF PLACENTAL PROTEIN 14 AS AN IMMUNOSUPPRESSIVE FACTOR IN HUMAN REPRODUCTIONThe Lancet, 329
D. Mukhopadhyay, Suma Sundereshan, C. Rao, A. Karande (2001)
Placental Protein 14 Induces Apoptosis in T Cells but Not in Monocytes*The Journal of Biological Chemistry, 276
Kazumichi Kuroda, H. Geyer, Rudolf Geyer, Walter Doerfler, H. Klenk (1990)
The oligosaccharides of influenza virus hemagglutinin expressed in insect cells by a baculovirus vector.Virology, 174 2
D. Harvey, G. Bousfield (2005)
Differentiation between sulphated and phosphated carbohydrates in low-resolution matrix-assisted laser desorption/ionization mass spectra.Rapid communications in mass spectrometry : RCM, 19 2
Rajesh Jayachandran, M. Shaila, A. Karande (2004)
Analysis of the Role of Oligosaccharides in the Apoptotic Activity of Glycodelin A*Journal of Biological Chemistry, 279
D. Harvey (2005)
Fragmentation of negative ions from carbohydrates: Part 2. Fragmentation of high-mannose N-linked glycansJournal of the American Society for Mass Spectrometry, 16
F. Matsuura, M. Ohta, Y. Ioannou, R. Desnick (1998)
Human alpha-galactosidase A: characterization of the N-linked oligosaccharides on the intracellular and secreted glycoforms overexpressed by Chinese hamster ovary cells.Glycobiology, 8 4
Vanessa Morais, Jacinta Serpa, Angelina Palma, Teresa Costa, Luís Maranga, Júlia Costa (2001)
Expression and characterization of recombinant human alpha-3/4-fucosyltransferase III from Spodoptera frugiperda (Sf9) and Trichoplusia ni (Tn) cells using the baculovirus expression system.The Biochemical journal, 353 Pt 3
M. Velardo, R. Bretthauer, A. Boutaud, B. Reinhold, V. Reinhold, F. Castellino (1993)
The presence of UDP-N-acetylglucosamine:alpha-3-D-mannoside beta 1,2-N-acetylglucosaminyltransferase I activity in Spodoptera frugiperda cells (IPLB-SF-21AE) and its enhancement as a result of baculovirus infection.The Journal of biological chemistry, 268 24
A. Dell, H. Morris, R. Easton, M. Panico, M. Patankar, S. Oehninger, R. Koistinen, H. Koistinen, M. Seppala, G. Clark (1995)
Structural Analysis of the Oligosaccharides Derived from Glycodelin, a Human Glycoprotein with Potent Immunosuppressive and Contraceptive Activities (*)The Journal of Biological Chemistry, 270
H. Koistinen, R. Koistinen, M. Seppälä, T. Burova, Y. Choiset, T. Haertlé (1999)
Glycodelin and β‐lactoglobulin, lipocalins with a high structural similarity, differ in ligand binding propertiesFEBS Letters, 450
H. Koistinen, R. Koistinen, Anne Dell, Howard Morris, R. Easton, Manish Patankar, S. Oehninger, Gary Clark, Markku Seppälä (1996)
Glycodelin from seminal plasma is a differentially glycosylated form of contraceptive glycodelin-A.Molecular human reproduction, 2 10
D. Harvey (1993)
Quantitative aspects of the matrix-assisted laser desorption mass spectrometry of complex oligosaccharides.Rapid communications in mass spectrometry : RCM, 7 7
F. Matsuura, M. Ohta, Y. Ioannou, R. Desnick (1998)
Human α-galactosidase A: characterization of the N-linked oligosaccharides on the intracellular and secreted glycoforms overexpressed by Chinese hamster ovary cellsGlycobiology, 8
Jason Rouse, A. Strang, Wen Yu, J. Vath (1998)
Isomeric differentiation of asparagine-linked oligosaccharides by matrix-assisted laser desorption-ionization postsource decay time-of-flight mass spectrometry.Analytical biochemistry, 256 1
I. Nieuwenhof, H. Koistinen, R. Easton, R. Koistinen, M. Kämäräinen, H. Morris, I. Die, M. Seppälä, A. Dell, D. Eijnden (2000)
Recombinant glycodelin carrying the same type of glycan structures as contraceptive glycodelin-A can be produced in human kidney 293 cells but not in chinese hamster ovary cells.European journal of biochemistry, 267 15
D. Mukhopadhyay, S. Sundarraj, A. Alok, A. Karande (2004)
Glycodelin A, Not Glycodelin S, Is Apoptotically ActiveJournal of Biological Chemistry, 279
K. Yamaguchi, K. Akai, G. Kawanishi, M. Ueda, S. Masuda, R. Sasaki (1991)
Effects of site-directed removal of N-glycosylation sites in human erythropoietin on its production and biological properties.The Journal of biological chemistry, 266 30
Clyde Stauffer (1975)
A linear standard curve for the Folin Lowry determination of protein.Analytical biochemistry, 69 2
P. Kulakosky, P. Hughes, H. Wood (1998)
N-Linked glycosylation of a baculovirus-expressed recombinant glycoprotein in insect larvae and tissue culture cells.Glycobiology, 8 7
N. Jenkins, E. Curling (1994)
Glycosylation of recombinant proteins: problems and prospects.Enzyme and microbial technology, 16 5
A. Wittwer, S. Howard (1990)
Glycosylation at Asn-184 inhibits the conversion of single-chain to two-chain tissue-type plasminogen activator by plasmin.Biochemistry, 29 17
H. Berman, Tammy Battistuz, T. Bhat, Wolfgang Bluhm, Philip Bourne, K. Burkhardt, Zukang Feng, G. Gilliland, L. Iype, Shri Jain, Phoebe Fagan, Jessica Marvin, David Padilla, V. Ravichandran, B. Schneider, N. Thanki, H. Weissig, J. Westbrook, C. Zardecki
Electronic Reprint Biological Crystallography the Protein Data Bank Biological Crystallography the Protein Data Bank
Bernhard Kuster, S. Wheeler, Ann Hunter, R. Dwek, David Harvey (1997)
Sequencing of N-linked oligosaccharides directly from protein gels: in-gel deglycosylation followed by matrix-assisted laser desorption/ionization mass spectrometry and normal-phase high-performance liquid chromatography.Analytical biochemistry, 250 1
GlycodelinA (GdA), a multifunctional glycoprotein secreted at high concentrations by the uterine endometrium during the early phases of pregnancy, carries glycan chains on asparagines at positions N28 and N63. GdA purified from amniotic fluid is known to be a suppressor of T-cell proliferation, an inducer of T-cell apoptosis, and an inhibitor of sperm–zona binding in contrast to its glycoform, glycodelinS (GdS), which is secreted by the seminal vesicles into the seminal plasma. The oligosaccharide chains of GdA terminate in sialic acid residues, whereas those of GdS are not sialylated but are heavily fucosylated. Our previous work has shown that the apoptogenic activity of GdA resides in the protein backbone, and we have also demonstrated the importance of sialylation for the manifestation of GdA-induced apoptosis. Recombinant glycodelin (Gd) expressed in the Sf21 insect cell line yielded an apoptotically active Gd; however, the same gene expressed in the insect cell line Tni produced apoptotically inactive Gd, as observed with the gene expressed in the Chinese hamster ovary (CHO) cell line and earlier in Pichia pastoris. Glycan analysis of the Tni and Sf21 cell line-expressed Gd proteins reveals differences in their glycan structures, which modulate the manifestation of apoptogenic activity of Gd. Through apoptotic assays carried out with the wild-type (WT) and glycosylation mutants of Gd expressed in Sf21 and Tni cells before and after mannosidase digestion, we conclude that the accessibility to the apoptogenic region of Gd is influenced by the size of the glycans.
Glycobiology – Oxford University Press
Published: Nov 19, 2006
Read and print from thousands of top scholarly journals.
Already have an account? Log in
Bookmark this article. You can see your Bookmarks on your DeepDyve Library.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Copy and paste the desired citation format or use the link below to download a file formatted for EndNote
Access the full text.
Sign up today, get DeepDyve free for 14 days.
All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.