Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Diversity of virus-like agents killing Microcystis aeruginosa in a hyper-eutrophic pond

Diversity of virus-like agents killing Microcystis aeruginosa in a hyper-eutrophic pond We demonstrated the existence of the diversity of virus-like agents killing Microcystis aeruginosa in a shallow hyper-eutrophic pond in Japan, but without specific virus isolation, once in October and twice in December 2001. The pond water was treated by chloroform, filtered throughout GF-75 filter (0.3 µm) and then incubated with axenic M. aeruginosa for 7 days. The cell density of M. aeruginosa decreased 10-fold in the course of the incubation. Results suggested that the active agent for cell death was virus-like, based upon its size fraction (from 30 kDa to 0.3 µm), sensitivity on heat-treatment, and evidence of protein protection of DNA during our extraction procedure. From our results of pulsed field gel electrophoresis (PFGE) analysis, two or four different-sized DNA bands appeared from the culture lysate in each experiment. In total, 10 different-sized bands (10–90 kb) were observed from the three sample dates, suggesting that multiple virus-like agents killing M. aeruginosa existed in this pond. To our knowledge, this is the first report of the existence of virus-like agents with different genomic size that kill M. aeruginosa. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Plankton Research Oxford University Press

Diversity of virus-like agents killing Microcystis aeruginosa in a hyper-eutrophic pond

Loading next page...
 
/lp/oxford-university-press/diversity-of-virus-like-agents-killing-microcystis-aeruginosa-in-a-ENyGIB4xBB

References (28)

Publisher
Oxford University Press
Copyright
© The Author 2006. Published by Oxford University Press. All rights reserved. For Permissions, please email: journals.permissions@oxfordjournals.org
ISSN
0142-7873
eISSN
1464-3774
DOI
10.1093/plankt/fbi128
Publisher site
See Article on Publisher Site

Abstract

We demonstrated the existence of the diversity of virus-like agents killing Microcystis aeruginosa in a shallow hyper-eutrophic pond in Japan, but without specific virus isolation, once in October and twice in December 2001. The pond water was treated by chloroform, filtered throughout GF-75 filter (0.3 µm) and then incubated with axenic M. aeruginosa for 7 days. The cell density of M. aeruginosa decreased 10-fold in the course of the incubation. Results suggested that the active agent for cell death was virus-like, based upon its size fraction (from 30 kDa to 0.3 µm), sensitivity on heat-treatment, and evidence of protein protection of DNA during our extraction procedure. From our results of pulsed field gel electrophoresis (PFGE) analysis, two or four different-sized DNA bands appeared from the culture lysate in each experiment. In total, 10 different-sized bands (10–90 kb) were observed from the three sample dates, suggesting that multiple virus-like agents killing M. aeruginosa existed in this pond. To our knowledge, this is the first report of the existence of virus-like agents with different genomic size that kill M. aeruginosa.

Journal

Journal of Plankton ResearchOxford University Press

Published: Apr 1, 2006

There are no references for this article.