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Conjugative DNA metabolism in Gram-negative bacteria

Conjugative DNA metabolism in Gram-negative bacteria AbstractBacterial conjugation in Gram-negative bacteria is triggered by a signal that connects the relaxosome to the coupling protein (T4CP) and transferosome, a type IV secretion system. The relaxosome, a nucleoprotein complex formed at the origin of transfer (oriT), consists of a relaxase, directed to the nic site by auxiliary DNA-binding proteins. The nic site undergoes cleavage and religation during vegetative growth, but this is converted to a cleavage and unwinding reaction when a competent mating pair has formed. Here, we review the biochemistry of relaxosomes and ponder some of the remaining questions about the nature of the signal that begins the process. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png FEMS Microbiology Reviews Oxford University Press

Conjugative DNA metabolism in Gram-negative bacteria

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References (189)

Publisher
Oxford University Press
Copyright
© 2009 Federation of European Microbiological Societies.
ISSN
0168-6445
eISSN
1574-6976
DOI
10.1111/j.1574-6976.2009.00195.x
pmid
19919603
Publisher site
See Article on Publisher Site

Abstract

AbstractBacterial conjugation in Gram-negative bacteria is triggered by a signal that connects the relaxosome to the coupling protein (T4CP) and transferosome, a type IV secretion system. The relaxosome, a nucleoprotein complex formed at the origin of transfer (oriT), consists of a relaxase, directed to the nic site by auxiliary DNA-binding proteins. The nic site undergoes cleavage and religation during vegetative growth, but this is converted to a cleavage and unwinding reaction when a competent mating pair has formed. Here, we review the biochemistry of relaxosomes and ponder some of the remaining questions about the nature of the signal that begins the process.

Journal

FEMS Microbiology ReviewsOxford University Press

Published: Jan 10, 2010

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