Abstract Phytoene synthase (PSY) is the first committed enzyme of the carotenoid biosynthesis pathway and the most important point of regulation. Carotenoids are precursors of abscisic acid (ABA) which mediates abiotic stress tolerance responses in plants. ABA activates the synthesis of its own precursors through induction of PSY expression. Carrot, a specie that accumulates very high amounts of carotenoids in its reserve root, has two PSY paralog genes that are expressed differentially in the root. In this work we determined that DcPSY2 expression is induced by salt stress and ABA. A DcPSY2 promoter fragment was obtained and characterized. Bioinformatic analysis showed the presence of three ABA responsive elements (ABRE). Through overexpressing pPSY2:GFP in Nicotiana tabacum we determined that all three ABRE are necessary for ABA response. In the carrot transcriptome we identified 3 AREB/ABF (DcAREB) transcription factor candidates which localize in the nucleus but only DcAREB3 is induced under ABA treatment in carrot root. We found that the AREB transcription factors bind to the carrot DcPSY2 promoter and transactivate the expression of reporter genes. We conclude that DcPSY2 is involved in ABA-mediated salt stress tolerance in carrot through the binding of AREB/ABF transcription factors to its promoter. DcPSY2, salt stress, carrot, abscisic acid, DcAREB transcription factors © The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Experimental Biology. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
Journal of Experimental Botany – Oxford University Press
Published: May 30, 2018
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