Microscopy in this issue

Microscopy in this issue Review TI Workbench, an integrated software package for electrophysiology and imaging Takafumi Inoue TI Workbench is a software package that serves as a control and analysis center for cellular imaging and electrophysiological experiments. It integrates the control of cellular imaging, covering charge-coupled device cameras and multi-photon microscope systems and electrophysiological devices, as well as sophisticated data analyses. Microscopy 2018 67:3 129–143; doi:10.1093/jmicro/dfy015 Review Microscopic analysis of sperm movement: links to mechanisms and protein components Kazuo Inaba and Kogiku Shiba Microscopic analyses of the sperm movements give basic information for practical research on productive medicine, animal livestock and fisheries science. In this review, microscopic recording and analyzing systems for rapid movements of sperm flagellar motility are overviewed, including the analysis by high-speed camera, stroboscopic illumination, and computer assisted data processing. Microscopy 2018 67:3 144–155; doi:10.1093/jmicro/dfy021 Article Evaluation of residual aberration in fifth-order geometrical aberration correctors Shigeyuki Morishita, Yuji Kohno, Fumio Hosokawa, Kazu Suenaga and Hidetaka Sawada We calculated residual aberrations of fifth-order aberration correctors that are double-hexapole and delta types of correctors. The calculations showed that sixth-order three-lobe aberration becomes dominant in these correctors. In the experiments, we quantitatively evaluated residual aberrations by using a low-voltage microscope equipped with delta correctors for probe- and image-forming systems. Microscopy 2018 67:3 156–163; doi:10.1093/jmicro/dfy009 Article Microstructural observation of casein micelles in milk by cryo-electron microscopy of vitreous sections (CEMOVIS) Takamichi Kamigaki, Yosiko Ito, Yuri Nishino and Atsuo Miyazawa The internal structure of casein micelles in raw milk was observed using cryo-electron microscopy of vitreous sections. The results confirmed the casein micelle diameter, the distribution of colloidal calcium phosphate (CCP), areas containing pores or cavities filled with water and some of the casein aggregates to connect with CCP. Microscopy 2018 67:3 164–170; doi:10.1093/jmicro/dfy012 Article Phase retrieval using through-focus images in Lorentz transmission electron microscopy Takahiro Tamura, Yukinori Nakane, Hiroshi Nakajima, Shigeo Mori, Ken Harada and Yoshizo Takai We report on phase retrieval by the maximum likelihood method using through-focus images in Lorentz transmission electron microscopy. The method was evaluated using simulated and experimentally obtained 32 through-focus images of magnetic bubbles. Consequently, we obtained the magnetic domain structures of the magnetic bubbles with high reliability. Microscopy 2018 67:3 171–177; doi:10.1093/jmicro/dfy014 Article Electric shielding films for biased TEM samples and their application to in situ electron holography Yuki Nomura, Kazuo Yamamoto, Tsukasa Hirayama and Koh Saitoh We developed a novel sample preparation method for transmission electron microscopy (TEM) to suppress superfluous electric fields leaked from biased TEM samples. Double layers of AlOx and carbon act as a ‘nano-shield’ to suppress 99% of the electric fields outside of the sample. Microscopy 2018 67:3 178–186; doi:10.1093/jmicro/dfy018 © The Author(s) 2018. Published by Oxford University Press on behalf of The Japanese Society of Microscopy. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/about_us/legal/notices) http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Microscopy Oxford University Press

Microscopy in this issue

Microscopy , Volume Advance Article (3) – Jun 1, 2018

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Publisher
Oxford University Press
Copyright
© The Author(s) 2018. Published by Oxford University Press on behalf of The Japanese Society of Microscopy. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com
ISSN
0022-0744
eISSN
1477-9986
D.O.I.
10.1093/jmicro/dfy026
Publisher site
See Article on Publisher Site

Abstract

Review TI Workbench, an integrated software package for electrophysiology and imaging Takafumi Inoue TI Workbench is a software package that serves as a control and analysis center for cellular imaging and electrophysiological experiments. It integrates the control of cellular imaging, covering charge-coupled device cameras and multi-photon microscope systems and electrophysiological devices, as well as sophisticated data analyses. Microscopy 2018 67:3 129–143; doi:10.1093/jmicro/dfy015 Review Microscopic analysis of sperm movement: links to mechanisms and protein components Kazuo Inaba and Kogiku Shiba Microscopic analyses of the sperm movements give basic information for practical research on productive medicine, animal livestock and fisheries science. In this review, microscopic recording and analyzing systems for rapid movements of sperm flagellar motility are overviewed, including the analysis by high-speed camera, stroboscopic illumination, and computer assisted data processing. Microscopy 2018 67:3 144–155; doi:10.1093/jmicro/dfy021 Article Evaluation of residual aberration in fifth-order geometrical aberration correctors Shigeyuki Morishita, Yuji Kohno, Fumio Hosokawa, Kazu Suenaga and Hidetaka Sawada We calculated residual aberrations of fifth-order aberration correctors that are double-hexapole and delta types of correctors. The calculations showed that sixth-order three-lobe aberration becomes dominant in these correctors. In the experiments, we quantitatively evaluated residual aberrations by using a low-voltage microscope equipped with delta correctors for probe- and image-forming systems. Microscopy 2018 67:3 156–163; doi:10.1093/jmicro/dfy009 Article Microstructural observation of casein micelles in milk by cryo-electron microscopy of vitreous sections (CEMOVIS) Takamichi Kamigaki, Yosiko Ito, Yuri Nishino and Atsuo Miyazawa The internal structure of casein micelles in raw milk was observed using cryo-electron microscopy of vitreous sections. The results confirmed the casein micelle diameter, the distribution of colloidal calcium phosphate (CCP), areas containing pores or cavities filled with water and some of the casein aggregates to connect with CCP. Microscopy 2018 67:3 164–170; doi:10.1093/jmicro/dfy012 Article Phase retrieval using through-focus images in Lorentz transmission electron microscopy Takahiro Tamura, Yukinori Nakane, Hiroshi Nakajima, Shigeo Mori, Ken Harada and Yoshizo Takai We report on phase retrieval by the maximum likelihood method using through-focus images in Lorentz transmission electron microscopy. The method was evaluated using simulated and experimentally obtained 32 through-focus images of magnetic bubbles. Consequently, we obtained the magnetic domain structures of the magnetic bubbles with high reliability. Microscopy 2018 67:3 171–177; doi:10.1093/jmicro/dfy014 Article Electric shielding films for biased TEM samples and their application to in situ electron holography Yuki Nomura, Kazuo Yamamoto, Tsukasa Hirayama and Koh Saitoh We developed a novel sample preparation method for transmission electron microscopy (TEM) to suppress superfluous electric fields leaked from biased TEM samples. Double layers of AlOx and carbon act as a ‘nano-shield’ to suppress 99% of the electric fields outside of the sample. Microscopy 2018 67:3 178–186; doi:10.1093/jmicro/dfy018 © The Author(s) 2018. Published by Oxford University Press on behalf of The Japanese Society of Microscopy. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/about_us/legal/notices)

Journal

MicroscopyOxford University Press

Published: Jun 1, 2018

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