Abstract Objective In order to investigate the molecular underpinnings of thyroid cancer, preclinical cell line models are crucial; however, ∼40% of these have been proven to be either duplicates of existing thyroid lines or even non-thyroid derived lines or are not derived from human at all. Therefore, we set out to establish procedures and guidelines that should proactively avoid these problems, which facilitated the creation of criteria to make valid pre-clinical models for thyroid cancer research. Design Based upon our recommendations, we systematically characterized all new cell lines that we generated by a standardized approach that included (i) determination of human origin, (ii) exclusion of lymphoma, (iii) DNA fingerprinting and histological comparisons to establish linkage to presumed tissue of origin, (iv) examining thyroid differentiation by screening 2-3 thyroid markers, (v) examination of biological behavior (growth rate, tumorigenicity) and (vi) presence of common thyroid cancer genetic changes (TP53, BRAF, PTEN, PIK3CA, RAS, TERT promoter, RET/PTC, PAX8/PPARγ, NF1 and EIF1AX). Results We established 7 new thyroid cell lines (LAM136, EAM306, SDAR1, SDAR2, JEM493, THJ529, and THJ560) out of 294 primary culture attempts, and 10 patient-derived tumor xenografts (PDTX; MC-Th-95, MC-Th-374, MC-Th-467, MC-Th-491, MC-Th-493, MC-Th-504, MC-Th-524, MC-Th-529, MC-Th-560, MC-Th-562) out of 67 attempts. All were successfully validated by our protocols. Conclusions This standardized approach for cell line and PDTX characterization should prevent (or detect) future cross-contamination and ensure that only valid preclinical models are used for thyroid cancer research. Copyright © 2018 Endocrine Society
Journal of Clinical Endocrinology and Metabolism – Oxford University Press
Published: May 25, 2018
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