Abstract Rubber trees are the major source of worldwide natural rubber. Rubber-containing latex is obtained from the laticifer cells of rubber tree (Hevea brasiliensis Muell. Arg.) via regular tapping. However, little is known about the positive feedback regulation of rubber biosynthesis, a typical isoprenoid metabolic process in laticifer cells, by tapping-caused loss of latex. Here, we demonstrated the crucial role of jasmonate signalling in this feedback regulation. The endogenous jasmonate levels, the expression levels of rubber biosynthesis-related genes and the efficiency of in vitro rubber biosynthesis were significantly higher in laticifer cells of regularly tapped trees than those of virgin trees. Application of methyl jasmonate had similar effects to latex exploitation in up-regulating the rubber biosynthesis-related genes and enhancing rubber biosynthesis. The specific jasmonate signalling module (COI1-JAZ3-MYC2) in laticifer cells of rubber tree was further identified. Its activation was associated with the enhanced natural rubber biosynthesis via up-regulating the expression of a farnesyl pyrophosphate synthase gene and a small rubber particle protein gene. The increase in the corresponding proteins, especially the farnesyl pyrophosphate synthase likely contributes to the increased efficiency of rubber biosynthesis. To our knowledge, this is the first study to reveal a jasmonate signalling pathway in regulating rubber biosynthesis in laticifer cells. The identification of the specific jasmonate signalling module in laticifer cells of rubber tree may provide a clue for genetic improvement of rubber yield potential. Hevea brasiliensis Muell Arg, jasmonate signalling, laticifer cell, isoprenoid metabolism, rubber biosynthesis, secondary metabolism © The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: email@example.com This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/about_us/legal/notices)
Journal of Experimental Botany – Oxford University Press
Published: May 3, 2018
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