Add Journal to My Library
Briefings in Bioinformatics
, Volume Advance Article – Oct 16, 2017

12 pages

/lp/ou_press/evaluation-of-variable-selection-methods-for-random-forests-and-omics-QjjIZQ8gLM

- Publisher
- Oxford University Press
- Copyright
- © The Author 2017. Published by Oxford University Press.
- ISSN
- 1467-5463
- eISSN
- 1477-4054
- D.O.I.
- 10.1093/bib/bbx124
- Publisher site
- See Article on Publisher Site

Abstract Machine learning methods and in particular random forests are promising approaches for prediction based on high dimensional omics data sets. They provide variable importance measures to rank predictors according to their predictive power. If building a prediction model is the main goal of a study, often a minimal set of variables with good prediction performance is selected. However, if the objective is the identification of involved variables to find active networks and pathways, approaches that aim to select all relevant variables should be preferred. We evaluated several variable selection procedures based on simulated data as well as publicly available experimental methylation and gene expression data. Our comparison included the Boruta algorithm, the Vita method, recurrent relative variable importance, a permutation approach and its parametric variant (Altmann) as well as recursive feature elimination (RFE). In our simulation studies, Boruta was the most powerful approach, followed closely by the Vita method. Both approaches demonstrated similar stability in variable selection, while Vita was the most robust approach under a pure null model without any predictor variables related to the outcome. In the analysis of the different experimental data sets, Vita demonstrated slightly better stability in variable selection and was less computationally intensive than Boruta. In conclusion, we recommend the Boruta and Vita approaches for the analysis of high-dimensional data sets. Vita is considerably faster than Boruta and thus more suitable for large data sets, but only Boruta can also be applied in low-dimensional settings. machine learning, random forest, feature selection, high dimensional data, relevant variables Introduction Owing to recent technological advances it is possible nowadays to characterize patients or healthy controls at multiple omics levels. For example, expression of >20 000 mRNA transcripts or the methylation status at >400 000 CpG sites in the genome can be measured using microarrays. Next-generation sequencing (NGS) technologies enable even larger numbers of molecules to be quantified. Although different technologies are used for different omics levels, the resulting data sets have several common characteristics making their analysis challenging. The number of variables is often much larger than the number of individuals. Furthermore, the data sets are usually sparse regarding relevant information, i.e. only a small set of variables is associated with the outcome. Additionally, complex correlation patterns are present between the variables. Machine learning methods are promising computational approaches for classification and regression utilizing high-dimensional omics data sets. A particularly well-suited method to tackle the presented challenges is random forest (RF) [1], an ensemble learning method based on decision trees. RF has been successfully applied in genetic [2], gene expression [3], methylation [4], proteomics [5] and metabolomics studies [6]. It is a flexible approach that can be used to both perform classification, i.e. predicting case-control status, and regression, i.e. predicting quantitative traits. In many applications a well-performing prediction model is only one of the goals. Another, sometimes even more important, aim is to identify those variables that enable this good prediction, i.e. to reduce the large set of measured variables to the ones that contain more information than noise. RFs provide variable importance measures, which can be used to rank variables based on their predictive importance. However, it is difficult to distinguish relevant from irrelevant variables based on this ranking only. Therefore, several variable selection procedures have been proposed that use different criteria and approaches to report the set of truly relevant variables. One popular approach that is also used in combination with other machine learning methods is recursive feature elimination (RFE) [7]. RFE uses the prediction error to select a minimal set of variables needed for a good prediction. Hence, only a limited number of variables need to be measured for further application of the prediction model. However, for many clinically relevant outcomes such as survival or response to treatment, different sets of variables with only few or even no common genes might lead to similar prediction performances [8, 9]. These sets might come from independent studies or are defined based on slightly different versions of the training data that are generated by bootstrapping or subsampling. The main reason for this finding is that many omics variables are correlated and therefore carry redundant information regarding prediction [10]. Including these redundant variables in the variable set will lead to more consistent results and provide a more complete picture of the pathological mechanisms driving disease or prognosis [9, 11]. In addition to the improved interpretability, prediction models based on larger sets of variables might have better prediction performance on independent data sets [12]. Evaluating prediction performance to determine important variables is suitable for approaches that aim to extract a minimal set of discriminative predictor variables like RFE. In contrast, alternative variable selection methods that do not remove redundant variables, estimate the distribution of importance values of irrelevant variables and select predictor variables with significantly larger importance values. These approaches are becoming more popular [13, 14] and our comparison study includes not only well-known methods such as permutation (Perm), its parametric variant (Altmann) [15] and the Boruta algorithm [16], but also recently published approaches like recurrent relative variable importance (r2VIM) [17] and the Vita method [18]. We perform a systematic comparison study using both simulated and experimental data. The two simulation setups contain correlated predictor variables. While the first study is based on simple correlation structures, more complex and realistic correlation patterns are estimated from experimental data and used in the second study. We also analyze experimental methylation and gene expression data sets using data from two independent studies for each classification setting. In each of the studies, prediction performance and stability of variable selection are assessed. In the simulation studies we additionally evaluate sensitivity, empirical power and false discovery rate (FDR; only Simulation Study 1). In the next section, we first explain the RF approach before we describe the different variable selection methods in more detail. We then present the simulation studies and the experimental data sets, followed by results and discussion. Random forest RF is an ensemble learning method based on classification and regression trees [1]. Each tree is trained on a bootstrap sample, and optimal variables at each split are identified from a random subset of all variables. The selecting criteria are different for classification and regression problems. For the former setting, the Gini index is applied, whereas variance reduction is used for the latter approach. The global prediction of the RF is calculated as a majority vote or average for classification or regression, respectively. Regression mode can also be used to obtain a binary outcome enabling estimation of probabilities similar to logistic regression [19]. In addition to prediction, RFs can be used to estimate variable importance measures to rank variables by predictive importance. One popular variable importance is the mean decrease of the Gini index. However, it has been shown that this method is biased [20] and therefore it is not included in our comparison study. Instead, the permutation importance of a variable is used, which is calculated as the difference of prediction performance before and after permuting the values of the variable averaged over all trees. In each tree, only out-of-bag observations, i.e. observations that were not used for training the forest, are included in the importance calculation. Variables that are relevant for prediction will have large importance values, whereas variables that are not associated with the outcome have values close to zero. Variable selection methods Different approaches have been proposed to identify the most important variables based on the ranking mentioned in the previous subsection. An overview of the characteristics and original implementations of the methods that are compared in this study is provided in Table 1. Table 1. Information about the different variable selection approaches that are compared Abbreviation Name Goal Approach R package Ref. Citationsa Altmann Altmann All relevant variables Permutation of outcome; parametric P-value R code on first author’s Web site (http://www.altmann.eu/documents/PIMP.R) Implemented in ranger package (https://cran.r-project.org/web/packages/ranger/index.html) and vita package (https://cran.r-project.org/web/packages/vita/index.html) [15] 40 Boruta Boruta All relevant variables Importance significantly larger than those of shadow variables Boruta (https://cran.r-project.org/web/packages/Boruta/index.html) [16] 103 Perm Permutation All relevant variables Permutations of outcome; nonparametric P-value No specific implementation for RF – – r2VIM Recurrent relative variable importance All relevant variables Relative importance based on minimal observed importance; several runs of RF r2VIM (http://research.nhgri.nih.gov/software/r2VIM) [17] 2 RFE Recursive feature elimination Minimal set RF with smallest error based on iterative removal of least important variables varSelRF (https://cran.r-project.org/web/packages/varSelRF/index.html) [7] 671 Vita Vita All relevant variables P-values based on empirical null distribution based on non-positive importance scores calculated using hold-out approach Vita (https://cran.r-project.org/web/packages/vita/index.html) Implemented in ranger package (https://cran.r-project.org/web/packages/ranger/index.html) [18] 0 Abbreviation Name Goal Approach R package Ref. Citationsa Altmann Altmann All relevant variables Permutation of outcome; parametric P-value R code on first author’s Web site (http://www.altmann.eu/documents/PIMP.R) Implemented in ranger package (https://cran.r-project.org/web/packages/ranger/index.html) and vita package (https://cran.r-project.org/web/packages/vita/index.html) [15] 40 Boruta Boruta All relevant variables Importance significantly larger than those of shadow variables Boruta (https://cran.r-project.org/web/packages/Boruta/index.html) [16] 103 Perm Permutation All relevant variables Permutations of outcome; nonparametric P-value No specific implementation for RF – – r2VIM Recurrent relative variable importance All relevant variables Relative importance based on minimal observed importance; several runs of RF r2VIM (http://research.nhgri.nih.gov/software/r2VIM) [17] 2 RFE Recursive feature elimination Minimal set RF with smallest error based on iterative removal of least important variables varSelRF (https://cran.r-project.org/web/packages/varSelRF/index.html) [7] 671 Vita Vita All relevant variables P-values based on empirical null distribution based on non-positive importance scores calculated using hold-out approach Vita (https://cran.r-project.org/web/packages/vita/index.html) Implemented in ranger package (https://cran.r-project.org/web/packages/ranger/index.html) [18] 0 aBased on Web of Science (24 July 2017). Table 1. Information about the different variable selection approaches that are compared Abbreviation Name Goal Approach R package Ref. Citationsa Altmann Altmann All relevant variables Permutation of outcome; parametric P-value R code on first author’s Web site (http://www.altmann.eu/documents/PIMP.R) Implemented in ranger package (https://cran.r-project.org/web/packages/ranger/index.html) and vita package (https://cran.r-project.org/web/packages/vita/index.html) [15] 40 Boruta Boruta All relevant variables Importance significantly larger than those of shadow variables Boruta (https://cran.r-project.org/web/packages/Boruta/index.html) [16] 103 Perm Permutation All relevant variables Permutations of outcome; nonparametric P-value No specific implementation for RF – – r2VIM Recurrent relative variable importance All relevant variables Relative importance based on minimal observed importance; several runs of RF r2VIM (http://research.nhgri.nih.gov/software/r2VIM) [17] 2 RFE Recursive feature elimination Minimal set RF with smallest error based on iterative removal of least important variables varSelRF (https://cran.r-project.org/web/packages/varSelRF/index.html) [7] 671 Vita Vita All relevant variables P-values based on empirical null distribution based on non-positive importance scores calculated using hold-out approach Vita (https://cran.r-project.org/web/packages/vita/index.html) Implemented in ranger package (https://cran.r-project.org/web/packages/ranger/index.html) [18] 0 Abbreviation Name Goal Approach R package Ref. Citationsa Altmann Altmann All relevant variables Permutation of outcome; parametric P-value R code on first author’s Web site (http://www.altmann.eu/documents/PIMP.R) Implemented in ranger package (https://cran.r-project.org/web/packages/ranger/index.html) and vita package (https://cran.r-project.org/web/packages/vita/index.html) [15] 40 Boruta Boruta All relevant variables Importance significantly larger than those of shadow variables Boruta (https://cran.r-project.org/web/packages/Boruta/index.html) [16] 103 Perm Permutation All relevant variables Permutations of outcome; nonparametric P-value No specific implementation for RF – – r2VIM Recurrent relative variable importance All relevant variables Relative importance based on minimal observed importance; several runs of RF r2VIM (http://research.nhgri.nih.gov/software/r2VIM) [17] 2 RFE Recursive feature elimination Minimal set RF with smallest error based on iterative removal of least important variables varSelRF (https://cran.r-project.org/web/packages/varSelRF/index.html) [7] 671 Vita Vita All relevant variables P-values based on empirical null distribution based on non-positive importance scores calculated using hold-out approach Vita (https://cran.r-project.org/web/packages/vita/index.html) Implemented in ranger package (https://cran.r-project.org/web/packages/ranger/index.html) [18] 0 aBased on Web of Science (24 July 2017). For better comparability in our study we reimplemented several of the approaches in a common framework that is based on the ranger R package [21]. This new implementation of RFs is more time and memory efficient compared with the standard RF implementation in the randomForest R package. Therefore it is better suited for large data sets that are common in omics studies. Our reimplementations are provided as an R package called Pomona (the Roman goddess of fruits) on GitHub (https://github.com/silkeszy/Pomona). Another alternative to the variable selection approaches included in our study is a method called VSURF [22], which is implemented in an R package with the same name [23]. However, the method seems to be sensitive to the parameter settings and it was not possible to analyze the experimental data sets in a reasonable amount of time (data not shown). Recursive feature elimination RFE aims to find a minimal set of variables, which leads to a good prediction model [7]. It starts with a RF built on all variables. A specific proportion of the least important variables is then removed and a new RF is generated using the remaining variables. These steps are recursively applied until a single variable is left as input. At each step the prediction performance is estimated based on the out-of-bag samples that were not used for model building. The set of variables that leads to the RF with the smallest error or to an error within a small range of the minimum is selected. The original method as implemented in the R package varSelRF calculates variable importance only once based on the RF with all variables. However, we recompute the rankings at each step because it has been shown that this modified algorithm is more efficient in case of correlated predictors [24]. RFE is a popular method for variable selection and it has been cited >500 times in Web of Science. It is often applied to analyze high-dimensional molecular data sets generated, e.g. in transcriptomics [25], proteomics [26] and metabolomics [27] experiments. Boruta The Boruta algorithm is named after a god of the forest in the Slavic mythology and was developed to identify all relevant variables within a classification framework [16]. The main idea of this approach is to compare the importance of the real predictor variables with those of random so-called shadow variables using statistical testing and several runs of RFs. In each run, the set of predictor variables is doubled by adding a copy of each variable. The values of those shadow variables are generated by permuting the original values across observations and therefore destroying the relationship with the outcome. A RF is trained on the extended data set and the variable importance values are collected. For each real variable a statistical test is performed comparing its importance with the maximum value of all the shadow variables. Variables with significantly larger or smaller importance values are declared as important or unimportant, respectively. All unimportant variables and shadow variables are removed and the previous steps are repeated until all variables are classified or a pre-specified number of runs has been performed. The original implementation based on the standard randomForest R package [28] was computationally intensive, which made its application in large omics data sets challenging. Nevertheless, the Boruta approach has been used in >100 studies, including omics data sets resulting from gene expression [29] and microbiome data analysis [30]. In the 5.0 version of the Boruta package, the ranger package is used for RF training and variable importance estimation. Permutation approach In contrast to the Boruta approach, where values of the predictor variables are permuted, a standard permutation test can be applied to estimate the distribution of importance scores under the null hypothesis of no association between predictor variables and outcome. The Perm approach therefore permutes values of the outcome, which leaves correlation patterns between predictor variables untouched. In our comparison, outcome permutations are repeated several times, and in each step, a RF is trained and variable importance values under the complete null hypothesis are estimated. Predictor variables with original importance values larger than importance values in all permutation runs are selected as important. Altmann Perm determines empirical (nonparametric) P-values based on the null distribution of variable importance scores after permuting the outcome. To reduce the number of permutations, Altmann et al. [15] proposed to use parametric P-values by fitting a defined probability distribution such as normal, lognormal or Gamma to the empirical distribution of null importance values. Parameters of these distributions are estimated using maximum likelihood methods and P-values are calculated as the probability of observing an importance score that is larger than the original importance score under the estimated distribution. In our comparison we used the normal distribution, which has been shown to work well in practice, see e.g. [18]. However, we would like to note that a different distribution might be more appropriate if a different variable importance score is used. For example, a Z score defined on a scaled version of the permutation importance is not normally distributed [31]. Furthermore, a statistical test based on these values has undesirable properties [32]. Although the Altmann approach was published in the same year as the Boruta approach, it has only been cited 40 times but used in several omics studies, e.g. analyzing gene expression [33] and microbiome data [34]. Recurrent relative variable importance The idea of r2VIM is based on the assumption that omics data sets usually contain many unimportant variables, which can be used to estimate importance values of null variables [17]. Several RFs are generated based on the same data set and parameter values differing only in the seed of the random number generating process. Each RF is used to calculate importance values, which are divided by the absolute minimal importance value observed in each run resulting in relative values that can be interpreted more easily. In case the minimal observed importance is exactly zero in a specific RF run, the minimal importance value over all runs is used as denominator. Variables with a minimal relative importance value greater or equal to a specified factor are selected and defined as important variables. To identify genetic variants that are associated with complex diseases, r2VIM has been developed [17]. In simulated genome-wide association data sets, it controlled the number of false positives if a factor of 3 or larger is used. Compared with standard statistical methods, such as logistic regression, the power to detect causal variants is only slightly decreased. The method can also be applied to identify gene–gene interactions [35]. Vita The Vita algorithm proposed by Janitza et al. [18] is similar to r2VIM, as it uses only the existing data without any permutations to estimate the null distribution of variable importance scores. The observed non-positive variable importance scores are used to construct a distribution that is symmetric around zero. The authors showed that variable importance values of null variables that are calculated using out-of-bag samples are positively skewed and a symmetric distribution is only achieved using a special cross-validation procedure (called hold-out approach). The overall data set is divided into two equally sized subsets, and two RFs are trained using either one of the sets. Variable importance is then estimated based on the other, independent set. The final importance values, called hold-out importance, are calculated by averaging the two estimated scores per variable. Based on the resulting empirical distribution, P-values can be calculated. Because this approach has been published recently, no studies citing the method could be identified. Performance comparison Because both experimental and simulated omics data provide specific advantages to compare the different variable selection methods, we used both types of data sets for our comparison. In simulation studies the true substructure in the data is known, and the application of statistical methods enables the estimation of power and FDRs. However, real experimental omics data sets feature complex properties that are difficult to simulate, e.g. usually they have complex correlation structures. Hence, investigation of experimental data is obligatory to obtain reliable results to evaluate statistical and computational methods under realistic analysis settings. In this examination we included two different simulation studies: In the first one the relationship between the quantitative outcome and a small number of predictor variables is nonlinear and therefore well suited for an analysis using the RF approach. In contrast, correlation between predictor variables is defined in a simple block structure. In addition, the data sets contain many uncorrelated variables that are independent of the outcome to analyze the risk of false-positive findings. In contrast, the second simulation study is based on a classification setting and more closely resembles omics data because correlation patterns estimated from experimental data were simulated. Because all of the predictor variables were strongly correlated with at least one of the other variables, it is not possible to evaluate false positives because selected variables are either directly influencing the outcome or are correlated with at least one of those variables. Simulation Study 1 Data For the simulated data in this study we used a modified version of a nonlinear regression model with correlated predictor variables, which has been used previously [36]. The quantitative outcome depends on three variables according to the formula y=0.25 exp (4x1)+41+exp -20x2-0.5+3x3+ε Where ε was normally distributed with mean 0 and standard deviation 0.2 N0,0.2. The three variables x1, x2 and x3 as well as three additional variables x4, x5 and x6 were independently sampled from a uniform distribution on the interval 0 and 1 ( U0,1) and used as basis for generating the correlated predictor variables. They were simulated according to vij=xi+0.01+0.5j-1n-1·N0,0.3 for j=1,…,n and i=1,…, 6, where vij denotes the j-th variable in group i and n is the size of each group. Note that correlation between the base variable xi and vij decreases as j increases. Furthermore, additional predictor variables that are uncorrelated with any of the base variables and each other were simulated based on a uniform distribution U0,1. We considered four different simulation scenarios: The first two included the causal variables vij,i=1,2,3 as well as the correlated, non-causal variables vij,i=4,5,6 and differed in group size n, for which we used the values 10 and 50. The two other scenarios use the same group sizes and are null models, i.e. the outcome does not depend on any of the simulated predictor variables. Here, variables xi,i=1,2,3 were only used to simulate correlated variables vij. The outcome was simulated based on three independent, normally distributed variables ( N0,0.2). For each of the four scenarios, 100 replicates with 100 individuals and a total number of 5000 predictor variables were simulated. The first 50 replicates were used for variable selection and training a final model, whereas the remaining 50 replicates were used for estimating prediction performance (see below). Parameters for RF and each method can be found in Table 2. Method-specific parameters were selected based on the analysis of the first simulation scenario so that the different approaches lead to similar FDRs (data not shown). Table 2. Parameters used for RF and variable selection methods Approach Parameter Description Value RF ntree Number of trees 10 000 mtry Number of variables selected at each split 33% of number of variables nodesize Minimal number of individuals in terminal node 10% of sample size Altmann no.perm Number of permutations 50 p.t Threshold for P-values 0 Borutaa pValue Confidence level 0.01 Perm no.perm Number of permutations 500 p.t Threshold for P-values 0 r2VIM no.runs Number of RFs to be generated 20 factor Minimal relative importance score for a variable to be selected 3 RFE prop.rm Proportion of variables removed at each step 0.1 tol Acceptable difference in optimal performance (in %) 10 Vita p.t Threshold for P-values 0 Approach Parameter Description Value RF ntree Number of trees 10 000 mtry Number of variables selected at each split 33% of number of variables nodesize Minimal number of individuals in terminal node 10% of sample size Altmann no.perm Number of permutations 50 p.t Threshold for P-values 0 Borutaa pValue Confidence level 0.01 Perm no.perm Number of permutations 500 p.t Threshold for P-values 0 r2VIM no.runs Number of RFs to be generated 20 factor Minimal relative importance score for a variable to be selected 3 RFE prop.rm Proportion of variables removed at each step 0.1 tol Acceptable difference in optimal performance (in %) 10 Vita p.t Threshold for P-values 0 aUsing default parameters (mtry = square root of number of variables; nodesize = 1 for classification, 5 for regression). Table 2. Parameters used for RF and variable selection methods Approach Parameter Description Value RF ntree Number of trees 10 000 mtry Number of variables selected at each split 33% of number of variables nodesize Minimal number of individuals in terminal node 10% of sample size Altmann no.perm Number of permutations 50 p.t Threshold for P-values 0 Borutaa pValue Confidence level 0.01 Perm no.perm Number of permutations 500 p.t Threshold for P-values 0 r2VIM no.runs Number of RFs to be generated 20 factor Minimal relative importance score for a variable to be selected 3 RFE prop.rm Proportion of variables removed at each step 0.1 tol Acceptable difference in optimal performance (in %) 10 Vita p.t Threshold for P-values 0 Approach Parameter Description Value RF ntree Number of trees 10 000 mtry Number of variables selected at each split 33% of number of variables nodesize Minimal number of individuals in terminal node 10% of sample size Altmann no.perm Number of permutations 50 p.t Threshold for P-values 0 Borutaa pValue Confidence level 0.01 Perm no.perm Number of permutations 500 p.t Threshold for P-values 0 r2VIM no.runs Number of RFs to be generated 20 factor Minimal relative importance score for a variable to be selected 3 RFE prop.rm Proportion of variables removed at each step 0.1 tol Acceptable difference in optimal performance (in %) 10 Vita p.t Threshold for P-values 0 aUsing default parameters (mtry = square root of number of variables; nodesize = 1 for classification, 5 for regression). Evaluation criteria We used the parameters FDR, sensitivity, empirical power, stability and root mean squared error (RMSE) to evaluate the performance of the different variable selection approaches. A false-positive finding means the selection of any predictor variable except the causal variables vij,i=1,2,3, by the variable selection method. FDR is defined as the frequency of false-positive findings among all variables selected per method and replicate. Sensitivity is defined as the proportion of correctly identified causal variables among all causal variables on a single replicate. The empirical power of each causal variable is calculated as the frequency of correct selections among all replicates. To estimate stability of variable selection, all pairwise combinations of replicates are considered. For each pair, the stability of the two lists of selected variables by the two replicates was determined using the Jaccard’s index, which is calculated as the division of the length of the intersection and the length of the union of the two sets of variables [10]. This index is 0 if the two sets do not overlap and 1 if the two sets contain the same variables. The average of all pairs is used as stability value for the particular method [37]. Note that stability in our setting is evaluated across independent replicates simulated under the same model, in contrast to other studies where stability is assessed regarding small changes in the original data set such as drawing bootstrap samples [10]. To evaluate prediction performance, a RF using only the selected variables is trained on the same replicate used for variable selection. The corresponding RMSE is then estimated using an independent replicate simulated only for this particular purpose. Simulation Study 2 Data For this simulation strategy the correlation structure of the predictor variables was obtained from experimental data as reported previously [18]. Here we estimated the covariance matrix of a gene expression data set from breast cancer patients containing 12 592 genes (see ‘Experimental data sets’ section for details). Gene expression data for 200 individuals were simulated by assuming a multivariate normal distribution with a mean vector of zeros and the estimated covariance matrix using the R package Umpire [38]. Subsequently, 200 causal variables were selected by randomly choosing 25 variables for every effect size from the set {−3, −2, −1, −0.5, 0.5, 1, 2, 3}. To create a binary outcome, the means of the chosen causal variables were changed according to the corresponding effect size in 100 of the 200 individuals. We simulated two pairs of replicates with identical causal variables and effect sizes. For each pair, each replicate was in turn used to select variables and to train a final model as well as to estimate prediction performance. We simulated 50 pairs of replicates resulting in 100 replicates in total, but we used only 20 pairs for the time-consuming approach Perm. Evaluation criteria We used the parameters empirical power, stability and classification error to evaluate the results of this simulation study. Empirical power was calculated separately for each absolute effect size as the frequency of correctly selected causal variables among all selected variables. Stability was determined using Jaccard’s index for each pair of replicates as described in Simulation Study 1 (see above). Classification error for each replicate in a pair was calculated based on the RF trained on the other replicate, and the mean error was reported for each pair. Experimental data sets Data For the investigation of experimental data, we focused on biological questions for which two large data sets with different individuals are available in public repositories. Using two data sets has several advantages. Stability of selected variables across the two data sets can be estimated and RF models based on selected variables can be trained on one of the data sets and tested on the other. Furthermore, it is not required to divide the data sets for this evaluation, which would decrease the number of individuals and therefore the power to identify biologically relevant variables. An overview of the selected studies and their characteristics is given in Table 3. Table 3. Overview of the experimental studies used for the comparison of variable selection approaches Name Data type Number of variables Study Outcome Sex Methylation 21 761 CpG positions Female Male Adkins 66 74 Mozhui 109 105 Breast cancer Gene expression 12 592 genes ER nega ER posb TCGA (array) 117 396 TCGA (RNA-Seqc) 120 398 Name Data type Number of variables Study Outcome Sex Methylation 21 761 CpG positions Female Male Adkins 66 74 Mozhui 109 105 Breast cancer Gene expression 12 592 genes ER nega ER posb TCGA (array) 117 396 TCGA (RNA-Seqc) 120 398 aEstrogen receptor-negative breast cancer. bEstrogen receptor-positive breast cancer. cRNA-sequencing. Table 3. Overview of the experimental studies used for the comparison of variable selection approaches Name Data type Number of variables Study Outcome Sex Methylation 21 761 CpG positions Female Male Adkins 66 74 Mozhui 109 105 Breast cancer Gene expression 12 592 genes ER nega ER posb TCGA (array) 117 396 TCGA (RNA-Seqc) 120 398 Name Data type Number of variables Study Outcome Sex Methylation 21 761 CpG positions Female Male Adkins 66 74 Mozhui 109 105 Breast cancer Gene expression 12 592 genes ER nega ER posb TCGA (array) 117 396 TCGA (RNA-Seqc) 120 398 aEstrogen receptor-negative breast cancer. bEstrogen receptor-positive breast cancer. cRNA-sequencing. The first research question deals with sex classification based on methylation data. With the different sex chromosomes in males and females as well as X chromosome inactivation in females, most of the CpG positions on the X chromosome should show methylation levels around 0.5 in females, whereas the values in males should be close to either 0 or 1. We used two studies that measure methylation levels using the Illumina 27k array in cord blood of newborns [39, 40]. Preprocessed beta values were downloaded from the NCBI GEO database [41] (accession numbers: GSE27317 and GSE64940). Probes that did not map uniquely to the human genome [42], contained either a common single nucleotide polymorphism in their probe sequence [42] or missing values were removed as well as individuals for which reported sex did not match sex estimated based on median methylation values for probes on the X chromosome. We restricted our analyses to the 21 761 CpG positions available in both data sets of which 815 are on the X chromosome outside of the pseudo-autosomal regions. For comparability, in each of the two studies, methylation values of each CpG position were divided into four different groups based on the quartiles and these ordinal values were used as predictor variables. The second research question is prediction of estrogen receptor status in breast cancer patients using gene expression data. We created two different data sets based on the technology that was used to measure gene expression in patients available in The Cancer Genome Atlas (TCGA) [43]. For about half of the patients, measurements based on a custom Agilent microarray were available, and for the remaining patients we used NGS-based expression data. Clinical information and normalized expression measurements of primary tumor samples were downloaded from GDAC Firehose (http://gdac.broadinstitute.org/) [44]. Genes with missing values (array) or zero counts in >10% of the samples (NGS) or multiple locations in the genome were removed. Read counts were log transformed (log2(x + 1)). Technology-specific identifiers were converted to Ensembl gene identifiers using the Bioconductor package AnnotationHub version 2.4.2 (Ensembl GRCh37.75). To enable a comparison between the two technologies, expression values of each gene were standardized to a mean of 0 and standard deviation of 1 separately in the two data sets. In total, 12 592 genes were available in both data sets and used in the analyses. Evaluation criteria For both research questions, variable selection using the different approaches was performed on each data set with each method, and a RF was trained with only the previously selected variables as predictor variables. The classification error of the other data set was then used to evaluate prediction performance. In addition, the stability of variable selection was assessed by comparing the two variable lists generated on the two data sets using the Jaccard’s index (see description in simulation study) [10]. For the breast cancer data, we additionally report a modified version of the Jaccard’s index. Owing to technological differences more genes were identified in the RNASeq data so that we used the minimum number of selected genes in the two data sets as denominator of the index. We used the same parameters for RF training and variable selection as in the simulation study (Table 2). The only difference between the analyses of simulated and experimental data was the mtry value in sex classification data sets, where we had to reduce it to the default value (= square root of number of variables) to estimate the distribution of null predictor variables in the r2VIM method. For the evaluation of run time, we used a single compute node of a computer cluster with 16 CPUs and 40 GB RAM. Results Simulation Study 1 Results for the two simulation scenarios with true effects in Simulation Study 1 are summarized in Figure 1. Figure 1. View largeDownload slide Performance comparison in Simulation Study 1 based on simulations with true effects. Shown are FDRs versus sensitivity of the scenarios with a group size of 10 (A) and 50 (B) as well as RMSE versus stability for the scenarios with a group size of 10 (C) and 50 (D). Each subfigure displays the median as well as the interquartile range over all 50 replicates of each method using different plotting symbols and colors. Figure 1. View largeDownload slide Performance comparison in Simulation Study 1 based on simulations with true effects. Shown are FDRs versus sensitivity of the scenarios with a group size of 10 (A) and 50 (B) as well as RMSE versus stability for the scenarios with a group size of 10 (C) and 50 (D). Each subfigure displays the median as well as the interquartile range over all 50 replicates of each method using different plotting symbols and colors. Each of the subfigures shows two evaluation criteria, and an optimal method would be located in the upper left corner, i.e. would have high sensitivity and low FDR. As expected, FDRs of the different methods are generally low; however, they are larger in the scenario with the smaller group size of 10, i.e. with only few causal variables. Only RFE and Altmann have a median FDR of zero in this setting, whereas r2VIM has a median FDR of nearly 0.09. In addition, this approach is the only one with a median FDR greater than zero in the simulation scenario with group size 50. Most of the false-positive variables were selected in a single replicate, while three variables were chosen in two replicates. The median sensitivity is about 60% for all methods in the scenario with group size 10. However, it is more variable in the scenarios with group size 50, i.e. 150 correlated causal variables. The most sensitive approaches are Boruta with a sensitivity of 79% and Vita with 62%, while the sensitivity of RFE is only 7%. The RMSE is larger in the scenario with more causal variables, but the approaches have all similar errors. The stability of variable selection, i.e. the overlap of selected variables between replicates, is similar across the different methods in the simulation scenario with group size 10 where RFE has a low stability of only 58% compared with values between 76 and 87% for the other methods. Moving to the simulation scenario with group size 50, the stability drops slightly for the top-performing methods such as Boruta and Vita. A more dramatic decrease is shown for RFE, which, as mentioned before, aims at selecting a minimum set of variables. Thus it is expected that the selected variables do not overlap between replicates. Sensitivity is a global measure across all causal variables, but it is also of interest to evaluate the power of the different approaches for each causal variable separately. Figure 2 therefore shows the empirical power for each of the 30 and 150 causal variables in the simulation scenarios with group size 10 and 50, respectively. Figure 2. View largeDownload slide Empirical power to select causal variables in Simulation Study 1. Shown is the empirical power of each causal variable in the simulation scenarios with group sizes of 10 with a total of 30 causal variables (A) and 50 with a total of 150 causal variables (B). Each of the variable selection approaches is given in a different color. Figure 2. View largeDownload slide Empirical power to select causal variables in Simulation Study 1. Shown is the empirical power of each causal variable in the simulation scenarios with group sizes of 10 with a total of 30 causal variables (A) and 50 with a total of 150 causal variables (B). Each of the variable selection approaches is given in a different color. Overall, empirical power decreases from 100% for the first variables in Group 1 to <10% for variables in Group 3. This finding is consistent with the simulated correlation between variables and outcome since variables in Group 1 and 2 were simulated to have a larger effect on the outcome. In addition, for some methods power decreases within groups, which can be explained by decreasing correlation with the base variable and therefore with the outcome. Boruta has the largest empirical power across settings and variable groups. In the simulation setting with group size 10, r2VIM is more powerful than Vita, whereas the latter method has more power for the lower correlated variables in Groups 1 and 2 for the setting with group size 50. Interestingly, Vita is the only method for which the first variable in Group 2 has a lower power than the last variable in Group 1. Note that this comparison cannot be performed for the Boruta method because most of the variables in the two groups are detected in all replicates. As expected, RFE is the least powerful method. Results for the two simulated null scenarios where the outcome is independent of any of the predictor variables are shown in Figure 3. Figure 3. View largeDownload slide Performance comparison in Simulation Study 1 based on null model. Shown are RMSE versus number of falsely selected variables of the scenarios with outcome simulated independently of any predictor variables using group sizes of 10 (A) and 50 (B). Each subfigure displays the median as well as the interquartile range over all 50 replicates of each method using different plotting symbols and color. Figure 3. View largeDownload slide Performance comparison in Simulation Study 1 based on null model. Shown are RMSE versus number of falsely selected variables of the scenarios with outcome simulated independently of any predictor variables using group sizes of 10 (A) and 50 (B). Each subfigure displays the median as well as the interquartile range over all 50 replicates of each method using different plotting symbols and color. In general, the RMSEs and the numbers of falsely selected variables are similar in the two scenarios with group sizes of 10 and 50. The RMSEs are much larger than in the settings with true effects but they are in the same range for all methods. In contrast, the median number of falsely selected variables ranges from 0 for Vita to 10 for Perm out of the 5000 simulated predictor variables. Boruta detects four to five variables on average. Simulation Study 2 The second simulation study was conducted to compare the performance of the variable selection methods under realistic correlation structures. The results are summarized in Figure 4. Figure 4. View largeDownload slide Performance comparison in Simulation Study 2. Shown are classification error versus stability (A) and empirical power depending on the absolute effect size (B). For classification error and stability the median as well as the interquartile range over all 50 pairs of replicates are displayed. For empirical power the median frequency per category of absolute effect size is given. Results for each method can be distinguished by plotting symbols and colors. Figure 4. View largeDownload slide Performance comparison in Simulation Study 2. Shown are classification error versus stability (A) and empirical power depending on the absolute effect size (B). For classification error and stability the median as well as the interquartile range over all 50 pairs of replicates are displayed. For empirical power the median frequency per category of absolute effect size is given. Results for each method can be distinguished by plotting symbols and colors. In Figure 4A the parameters classification error and stability are displayed. Again, RFE is different from the other methods. The classification error is low but considerably larger than the classification error of the other methods that achieve a perfect classification. Median stability is zero meaning that different sets of variables are selected in each pair of replicates, which are simulated using the same causal variables and effect sizes. In contrast, the stability of the other methods ranges from around 70% to close to 100%. With a stability of approximately 95%, r2VIM is the most stable approach, followed by Boruta, Vita, Perm and Altmann. The empirical power as a function of absolute effect size is shown in Figure 4B. Variables with an effect size of 0.5 are never selected by any of the methods. In contrast, variables with the highest effect size of 3 are always selected by all methods except RFE. For variables with moderate effect sizes Boruta outperforms all methods. The second most powerful approach is Vita, which is similar to r2VIM concerning the larger effect size of 2. Empirical power of RFE is generally low and only variables with large effect sizes are selected. Experimental data sets Because the causal predictor variables are unknown in the experimental data sets the different variable selection methods can only be compared based on classification error and stability of variable selection as shown in Figure 5. Stability is determined here by comparing the two available data sets for each condition. Figure 5. View largeDownload slide Performance comparison based on experimental data sets. Shown are classification error versus stability of the two experimental studies predicting sex (A) and estrogen receptor positive breast cancer (B). Each subfigure displays the median error and variable stability of the two different data sets that were analyzed for each research question using different plotting symbols and shades of gray. Note that a different definition of stability is used in subfigure (B), which is defined relative to the minimum and not the union of the two sets of selected variables. Figure 5. View largeDownload slide Performance comparison based on experimental data sets. Shown are classification error versus stability of the two experimental studies predicting sex (A) and estrogen receptor positive breast cancer (B). Each subfigure displays the median error and variable stability of the two different data sets that were analyzed for each research question using different plotting symbols and shades of gray. Note that a different definition of stability is used in subfigure (B), which is defined relative to the minimum and not the union of the two sets of selected variables. In the sex classification problem, all methods, except RFE, show similar good prediction performances with classification errors <0.01. In addition, the stability of variable selection is approximately 85% for these methods. In contrast, RFE is unstable, owing to high correlation between different CpG positions on the X chromosome. Only two positions in each data set are selected by RFE and because they are different, the resulting stability of this method is zero (Supplementary Table S1). In the breast cancer problem, the classification error is larger but similar for all methods. Most methods select considerably more of the 12 592 common genes in the RNA-Seq data set compared with the array data set (Supplementary Table S1). Hence, a different definition of stability was used based on the minimum instead of the union of the two sets of selected variables. Based on this criterion, Vita is the most stable method with a stability of 58%, followed by Boruta with 42%. If the original stability definition is used all methods feature stability values <20% (Supplementary Figure S1). Supplementary Tables S2 and S3 list the selected CpG positions and genes per data set and method. Supplementary Figure S2 shows the overlap between selected variables across the different methods. In the sex classification problem, most of the CpG positions have been identified using several methods but Vita has selected >100 unique variables. And Vita is the only approach that uniquely detected three CpG sites on the autosomes. All other CpG sites are located on the X chromosome and can therefore be considered to be true findings. Prediction of estrogen receptor positive breast cancer results in more diverse sets of selected genes across methods. Only two genes have been identified by every method. One is the gene ESR1 (estrogen receptor 1), which encodes the estrogen receptor-alpha. The second gene is AGR3 (Anterior Gradient 3), which has been associated with estrogen receptor status in breast cancer at gene and protein expression levels [45, 46]. Figure 6 compares the average run time of each method for the two research questions. Figure 6. View largeDownload slide Run time comparison based on the classification of experimental data sets. Shown are run times (in hours) for each method as an average of the two data sets of each research question. Figure 6. View largeDownload slide Run time comparison based on the classification of experimental data sets. Shown are run times (in hours) for each method as an average of the two data sets of each research question. As expected, Perm is time-consuming for large omics data sets with >70 h of run time on the breast cancer data sets. Although the analysis of the sex classification problem took only approximately 8 h, Perm was still the slowest approach in our comparison. In contrast, Boruta finished within 2 and 1 h, respectively, while Vita was even more efficient with <6 and 2 min, respectively. Discussion and conclusion We compared different variable selection approaches that were recently proposed to identify all relevant predictor variables within a classification or regression problem. In addition, we included in our comparison a popular method with a different aim, i.e. to find a small subset of important variables with an optimal prediction performance. Both simulation studies identified Boruta as the most powerful approach, followed by the Vita method. Under a pure null model without any predictor variables related to the outcome, Vita was the most robust approach. Simulation studies were designed so that performance differences between the variable selection methods were observed. In contrast, evaluation criteria of all approaches except RFE were similar in the analysis of the experimental data sets. However, Vita demonstrated slightly better stability in variable selection than Boruta. The large overlap of selected CpG positions in the sex classification example is caused by the strong signal in the data sets because most of the positions on the X chromosome show large methylation differences between females and males. Effects are smaller and more variable in the breast cancer data sets so that results of the different methods are less consistent. For the analysis of large omics data sets we recommend Boruta and Vita, which is considerably faster. Because the different approaches use the same implementation of the RF algorithm, the differences in run time are owing to the number of RFs that are built with each method. For example, the Vita approach generates two forests, whereas the Perm method creates a complete new forest for each permutation. Using Vita, selecting variables and training the final RF model in a data set with 12 000 genes and 500 individuals was finished in <10 min. Thus, this approach will also be feasible for much larger data sets, e.g. measuring more than a million CpG positions in sequencing-based methylation studies, genetic variants genotyped on microarrays or using NGS. However, Vita, similar to r2VIM, estimates the null distribution of variable importance using provided predictor variables only. Therefore, this approach is only applicable in the setting of high-dimensional data sets where the assumption is valid that many variables are not important for predicting the outcome. In contrast, Boruta uses the concept of shadow variables, i.e. permuted versions of the original variables, and can therefore also be applied on the more traditional low-dimensional data with many more individuals than variables. Both, Boruta and Vita have a single and easily interpretable parameter, which defines a threshold for the internally calculated empirical P-values to identify relevant variables. In contrast, the other methods have two arguments, which need to be set by the user or alternatively tuned using e.g. cross-validation procedures. Some of the methods might not be robust regarding those parameter values, which might lead to diverse results (data not shown). A popular alternative to the RF approach for variable selection is penalized regression methods (also called regularized or shrinkage regression methods) such as Least Absolute Shrinkage and Selection Operator [47] or elastic net [48], which have been applied to omics data sets [49, 50]. The general idea is to add a penalty to the loss function so that regression coefficients are shrunken toward zero resulting in a sparse model. The performance of different types of penalized regression methods has been evaluated in several studies, e.g. [51, 52]; however, to the best of our knowledge, no comprehensive and neutral study comparing RF and penalized regression methods has been performed regarding selection of all relevant variables, which was the focus of the current study. As shown previously, the different variable selection methods lead to models with similar prediction performance; however, the sets of selected variables are different [28]. However, agreement and stability of variable selection depend strongly on the effect size of the predictor variables. Large sets of overlapping variables can be found if many predictor variables show strong association with the outcome as in the sex prediction problem based on methylation data. The results of the simulation studies are similar for both regression and classification settings, and the power of the methods is mainly influenced by the effect sizes of the predictor variables. Both simulation models contained some causal variables with small effects, which were not detected with reasonable power by any of the methods. The small overlap in selected genes in the experimental breast cancer data sets might as well be caused by a power problem. However, stability of variable selection might be improved if external information about biological pathways and networks can be integrated in the model training step [45]. In this situation, stability would not be defined on variable level such as genes but rather on pathways or modules in networks. In conclusion, we recommend Boruta and Vita for selection of relevant variables in high-dimensional data sets. The Boruta approach can also be applied in low-dimensional settings. Key Points For interpretation of machine learning prediction methods it is important to identify all relevant variables including those carrying redundant information. We performed a systematic evaluation of several variable selection approaches using both simulated and experimental data sets. The Boruta and Vita approaches are sensitive regarding detection of causal variables while controlling the number of false-positive findings at a reasonable level. Both methods are available as efficient implementations making analysis of high-dimensional data sets feasible. Boruta can also be applied in low-dimensional data sets with more observations than variables. Supplementary Data Supplementary data are available online at http://bib.oxfordjournals.org/. Frauke Degenhardt is a PhD student at the Institute of Clinical Molecular Biology, Kiel University, Germany. She analyzes data from inflammatory disease studies using standard statistical approaches as well as machine learning methods. Stephan Seifert PhD, is a postdoctoral researcher in a junior research group on systems medicine at the Institute of Medical Informatics and Statistics, Kiel University, Germany. In his PhD thesis he analyzed various pollen data with multivariate statistics in the Kneipp group at Humboldt-Universität in Berlin, Germany. Silke Szymczak PhD, is head of a junior research group on systems medicine at the Institute of Medical Informatics and Statistics, Kiel University. The group's research is focused on the development and evaluation of machine learning approaches for the analysis of omics data. Acknowledgements We thank Astrid Dempfle and Sandra Freitag-Wolf for their constructive comments that greatly improved the manuscript. The results published here are in part based on data generated by the TCGA Research Network: http://cancergenome.nih.gov/. Funding The German Federal Ministry of Education and Research (BMBF) within the framework of the e:Med research and funding concept (01ZX1510 to S.Szy. and S.Se.). References 1 Breiman L. Random forests . Mach Learn 2001 ; 45 : 5 – 32 . Google Scholar CrossRef Search ADS 2 Szymczak S , Biernacka JM , Cordell HJ , et al. Machine learning in genome-wide association studies . Genet Epidemiol 2009 ; 33 : S51 – 7 . Google Scholar CrossRef Search ADS PubMed 3 Alexe G , Monaco J , Doyle S , et al. Towards improved cancer diagnosis and prognosis using analysis of gene expression data and computer aided imaging . Exp Biol Med 2009 ; 234 : 860 – 79 . Google Scholar CrossRef Search ADS 4 Wilhelm T. Phenotype prediction based on genome-wide DNA methylation data . BMC Bioinformatics 2014 ; 15 : 193. Google Scholar CrossRef Search ADS PubMed 5 Swan AL , Mobasheri A , Allaway D , et al. Application of machine learning to proteomics data: classification and biomarker identification in postgenomics biology . Omics 2013 ; 17 : 595 – 610 . Google Scholar CrossRef Search ADS PubMed 6 Smolinska A , Hauschild A-C , Fijten R , et al. Current breathomics—a review on data pre-processing techniques and machine learning in metabolomics breath analysis . J Breath Res 2014 ; 8 : 027105. Google Scholar CrossRef Search ADS PubMed 7 Díaz-Uriarte R , De Andres SA. Gene selection and classification of microarray data using random forest . BMC Bioinformatics 2006 ; 7 : 3 . Google Scholar CrossRef Search ADS PubMed 8 Drier Y , Domany E. Do two machine-learning based prognostic signatures for breast cancer capture the same biological processes? PLoS One 2011 ; 6 : e17795. Google Scholar CrossRef Search ADS PubMed 9 Ein-Dor L , Kela I , Getz G , et al. Outcome signature genes in breast cancer: is there a unique set? . Bioinformatics 2005 ; 21 : 171 – 8 . Google Scholar CrossRef Search ADS PubMed 10 He Z , Yu W. Stable feature selection for biomarker discovery . Comput Biol Chem 2010 ; 34 : 215 – 25 . Google Scholar CrossRef Search ADS PubMed 11 Wang Y , Li Z , Wang Y , et al. A novel approach for stable selection of informative redundant features from high dimensional fMRI data . arXiv preprint arXiv:1506.08301 2015. 12 Yang P , Zhou BB , Yang JY-H , et al. Stability of feature selection algorithms and ensemble feature selection methods in bioinformatics . In: Elloumi M, Zomaya AY (eds). Biological Knowledge Discovery Handbook: Preprocessing, Mining and Postprocessing of Biological Data . Hoboken, New Jersey : John Wiley and Sons , 2013 , 333 – 52 . Google Scholar CrossRef Search ADS 13 Nilsson R , Peña JM , Björkegren J , et al. Consistent feature selection for pattern recognition in polynomial time . J Mach Learn Res 2007 ; 8 : 589 – 612 . 14 Rudnicki WR , Wrzesie M , Paja W. All relevant feature selection methods and applications. In: Stańczyk U, Jain L (eds). Feature Selection for Data and Pattern Recognition . Berlin Heidelberg, Germany : Springer , 2015 , 11 – 28 . 15 Altmann A , Tolosi L , Sander O , et al. Permutation importance: a corrected feature importance measure . Bioinformatics 2010 ; 26 : 1340 – 7 . Google Scholar CrossRef Search ADS PubMed 16 Kursa M , Rudnicki W. Feature selection with the Boruta package . J Stat Softw 2010 ; 36 : 1 – 13 . Google Scholar CrossRef Search ADS 17 Szymczak S , Holzinger E , Dasgupta A , et al. r2VIM: a new variable selection method for random forests in genome-wide association studies . BioData Min 2016 ; 9 : 1. Google Scholar CrossRef Search ADS PubMed 18 Janitza S , Celik E , Boulesteix A-L. A computationally fast variable importance test for random forests for high-dimensional data . Adv Data Anal Classif 2016 , in press. 19 Malley JD , Kruppa J , Dasgupta A , et al. Probability machines: consistent probability estimation using nonparametric learning machines . Methods Inf Med 2012 ; 51 : 74 – 81 . Google Scholar CrossRef Search ADS PubMed 20 Nicodemus KK , Malley JD. Predictor correlation impacts machine learning algorithms: implications for genomic studies . Bioinformatics 2009 ; 25 : 1884 – 90 . Google Scholar CrossRef Search ADS PubMed 21 Wright MN , Ziegler A. Ranger: a fast implementation of random forests for high dimensional data in C ++ and R. J Stat Softw 2017 ; 77 : 1 – 17 . 22 Genuer R , Poggi J-M , Tuleau-Malot C. Variable selection using random forests . Pattern Recognit Lett 2010 ; 31 : 2225 – 36 . Google Scholar CrossRef Search ADS 23 Genuer R , Poggi J-M , Tuleau-Malot C. VSURF: an R package for variable selection using random forests . R J 2015 ; 7 : 19 – 33 . 24 Gregorutti B , Michel B , Saint-Pierre P. Correlation and variable importance in random forests . Stat Comput 2013 ; 27 : 659 – 78 . Google Scholar CrossRef Search ADS 25 Habermann JK , Doering J , Hautaniemi S , et al. The gene expression signature of genomic instability in breast cancer is an independent predictor of clinical outcome . Int J Cancer 2009 ; 124 : 1552 – 64 . Google Scholar CrossRef Search ADS PubMed 26 Fusaro VA , Mani D , Mesirov JP , et al. Prediction of high-responding peptides for targeted protein assays by mass spectrometry . Nat Biotechnol 2009 ; 27 : 190 – 8 . Google Scholar CrossRef Search ADS PubMed 27 Dietrich S , Floegel A , Weikert C , et al. Identification of serum metabolites associated with incident hypertension in the European Prospective Investigation into Cancer and Nutrition–Potsdam Study . Hypertension 2016 ; 68 : 471 – 7 . Google Scholar CrossRef Search ADS PubMed 28 Kursa MB. Robustness of random forest-based gene selection methods . BMC Bioinformatics 2014 ; 15 : 8 . Google Scholar CrossRef Search ADS PubMed 29 Guo P , Luo Y , Mai G , et al. Gene expression profile based classification models of psoriasis . Genomics 2014 ; 103 : 48 – 55 . Google Scholar CrossRef Search ADS PubMed 30 Saulnier DM , Riehle K , Mistretta T , et al. Gastrointestinal microbiome signatures of pediatric patients with irritable bowel syndrome . Gastroenterology 2011 ; 141 : 1782 – 91 . Google Scholar CrossRef Search ADS PubMed 31 Rudnicki WR , Kierczak M , Koronacki J , et al. A statistical method for determining importance of variables in an information system. In: Yao J, Yang Y, Slowiński R, et al. (eds). International Conference on Rough Sets and Current Trends in Computing. Berlin Heidelberg, Germany : Springer , 2006 , 557 – 66 . 32 Strobl C , Zeileis A. Danger: high power!–exploring the statistical properties of a test for random forest variable importance. Department of Statistics University of Munich Technical Report 017, 2008 . http://epub.ub.uni-muenchen.de/2111/2008. 33 Ji S , Fakhry A , Deng H. Integrative analysis of the connectivity and gene expression atlases in the mouse brain . Neuroimage 2014 ; 84 : 245 – 53 . Google Scholar CrossRef Search ADS PubMed 34 Ning J , Beiko RG. Phylogenetic approaches to microbial community classification . Microbiome 2015 ; 3 : 1 . Google Scholar CrossRef Search ADS PubMed 35 Holzinger ER , Szymczak S , Dasgupta A , et al. Variable selection method for the identification of epistatic models . Pac Symp Biocomput 2014 ; 20 : 195 – 206 . 36 Chen Z , Zhang W. Integrative analysis using module-guided random forests reveals correlated genetic factors related to mouse weight . PLoS Comput Biol 2013 ; 9 : e1002956. Google Scholar CrossRef Search ADS PubMed 37 Boulesteix A-L , Slawski M. Stability and aggregation of ranked gene lists . Brief Bioinform 2009 ; 10 : 556 – 68 . Google Scholar CrossRef Search ADS PubMed 38 Zhang J , Coombes KR. Simulating gene expression data to estimate sample size for class and biomarker discovery . Int J Adv Life Sci 2012 ; 4 : 44 – 51 . 39 Adkins RM , Thomas F , Tylavsky FA , et al. Parental ages and levels of DNA methylation in the newborn are correlated . BMC Med Genet 2011 ; 12 : 47. Google Scholar CrossRef Search ADS PubMed 40 Mozhui K , Smith AK , Tylavsky FA. Ancestry dependent DNA methylation and influence of maternal nutrition . PLoS One 2015 ; 10 : e0118466. Google Scholar CrossRef Search ADS PubMed 41 Barrett T , Wilhite SE , Ledoux P , et al. NCBI GEO: archive for functional genomics data sets—update . Nucleic Acids Res 2013 ; 41 : D991 – 5 . Google Scholar CrossRef Search ADS PubMed 42 Chen Y , Choufani S , Ferreira JC , et al. Sequence overlap between autosomal and sex-linked probes on the Illumina HumanMethylation27 microarray . Genomics 2011 ; 97 : 214 – 22 . Google Scholar CrossRef Search ADS PubMed 43 Network CGA . Comprehensive molecular portraits of human breast tumours . Nature 2012 ; 490 : 61 – 70 . CrossRef Search ADS PubMed 44 Center BITGDA . Analysis-ready standardized TCGA data from Broad GDAC Firehose 2016_01_28 run. Broad Institute of MIT and Harvard. Dataset, 2016 . 45 Garczyk S , Stillfried S. v , Antonopoulos W , et al. AGR3 in breast cancer: prognostic impact and suitable serum-Based biomarker for early cancer detection . PLoS One 2015 ; 10 : e0122106. Google Scholar CrossRef Search ADS PubMed 46 Obacz J , Brychtova V , Podhorec J , et al. Anterior gradient protein 3 is associated with less aggressive tumors and better outcome of breast cancer patients . Onco Targets Ther 2015 ; 8 : 1523 . Google Scholar PubMed 47 Tibshirani R. Regression shrinkage and selection via the lasso . J R Stat Soc Series B Stat Methodol 1996 ; 58 : 267 – 88 . 48 Zou H , Hastie T. Regularization and variable selection via the elastic net . J R Stat Soc Series B Stat Methodol 2005 ; 67 : 301 – 20 . Google Scholar CrossRef Search ADS 49 Pineda S , Real FX , Kogevinas M , et al. Integration analysis of three omics data using penalized regression methods: an application to bladder cancer . PLoS Genet 2015 ; 11 : e1005689. Google Scholar CrossRef Search ADS PubMed 50 Wu TT , Chen YF , Hastie T , et al. Genome-wide association analysis by lasso penalized logistic regression . Bioinformatics 2009 ; 25 : 714 – 21 . Google Scholar CrossRef Search ADS PubMed 51 Neto EC , Bare JC , Margolin AA. Simulation studies as designed experiments: the comparison of penalized regression models in the “large p, small n” setting . PLoS One 2014 ; 9 : e107957. Google Scholar CrossRef Search ADS PubMed 52 Waldron L , Pintilie M , Tsao M-S , et al. Optimized application of penalized regression methods to diverse genomic data . Bioinformatics 2011 ; 27 : 3399 – 406 . Google Scholar CrossRef Search ADS PubMed © The Author 2017. Published by Oxford University Press. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

Briefings in Bioinformatics – Oxford University Press

**Published: ** Oct 16, 2017

Loading...

personal research library

It’s your single place to instantly

**discover** and **read** the research

that matters to you.

Enjoy **affordable access** to

over 18 million articles from more than

**15,000 peer-reviewed journals**.

All for just $49/month

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Read from thousands of the leading scholarly journals from *SpringerNature*, *Elsevier*, *Wiley-Blackwell*, *Oxford University Press* and more.

All the latest content is available, no embargo periods.

## “Hi guys, I cannot tell you how much I love this resource. Incredible. I really believe you've hit the nail on the head with this site in regards to solving the research-purchase issue.”

Daniel C.

## “Whoa! It’s like Spotify but for academic articles.”

@Phil_Robichaud

## “I must say, @deepdyve is a fabulous solution to the independent researcher's problem of #access to #information.”

@deepthiw

## “My last article couldn't be possible without the platform @deepdyve that makes journal papers cheaper.”

@JoseServera

DeepDyve ## Freelancer | DeepDyve ## Pro | |
---|---|---|

Price | FREE | $49/month |

Save searches from | ||

Create lists to | ||

Export lists, citations | ||

Read DeepDyve articles | Abstract access only | Unlimited access to over |

20 pages / month | ||

PDF Discount | 20% off | |

Read and print from thousands of top scholarly journals.

System error. Please try again!

or

By signing up, you agree to DeepDyve’s Terms of Service and Privacy Policy.

Already have an account? Log in

Bookmark this article. You can see your Bookmarks on your DeepDyve Library.

To save an article, **log in** first, or **sign up** for a DeepDyve account if you don’t already have one.

All DeepDyve websites use cookies to improve your online experience. They were placed on your computer when you launched this website. You can change your cookie settings through your browser.

ok to continue