Endothelial Nitric Oxide Synthase Expression in Tumor Vasculature Is Correlated with Malignancy in Human Supratentorial Astrocytic Tumors

Endothelial Nitric Oxide Synthase Expression in Tumor Vasculature Is Correlated with Malignancy... AbstractOBJECTIVE:Endothelial nitric oxide synthase (eNOS) may play an important role in the regulation of tumor blood flow and vascular permeability. However, there have been no reports describing alterations of eNOS expression in relation to malignant progression in human astrocytic tumors. We immunohistochemically studied the relationship between eNOS expression in tumor vasculature and malignancy in supratentorial astrocytic tumorsMETHODS:Tissue samples were obtained from 12 patients with low-grade astrocytomas, 10 with anaplastic astrocytomas, and 17 with glioblastomas. Normal brain tissue samples were obtained from four patients with other brain diseases. Immunohistochemical staining was performed using the avidin-biotin complex method, with polyclonal anti-eNOS antibody, and the levels of eNOS expression in endothelial cells were evaluated as slight, moderate, or intense on the basis of eNOS immunoreactivity. The proliferative potential was assessed as the MIB-1 staining index for tumor cells.RESULTS:The expression of eNOS was slight in all specimens of normal brain tissue, slight in 7 and moderate in 5 specimens of low-grade astrocytoma, slight in 2, moderate in 6, and intense in 2 specimens of anaplastic astrocytoma, and moderate in 5 and intense in 12 specimens of glioblastoma. The MIB-1 staining index (mean ± standard deviation) was 0.2 ± 0.2% for normal specimens, 1.8 ± 0.6% for low-grade astrocytomas, 9.6 ± 6.9% for anaplastic astrocytomas, and 18.5 ± 7.7% for glioblastomas. The MIB-1 staining indices for slight, moderate, and intense eNOS expression were 2.0 ± 2.3%, 10.8 ± 9.8%, and 16.9 ± 7.7%, respectivelyCONCLUSION:Expression of eNOS in tumor vessels was significantly correlated with histological grade and proliferative potential. These findings suggest that astrocytic tumor vessels possess higher activity for nitric oxide production than do normal vessels. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Neurosurgery Oxford University Press

Endothelial Nitric Oxide Synthase Expression in Tumor Vasculature Is Correlated with Malignancy in Human Supratentorial Astrocytic Tumors

Endothelial Nitric Oxide Synthase Expression in Tumor Vasculature Is Correlated with Malignancy in Human Supratentorial Astrocytic Tumors

CLINICAL STUDIES Endothelial Nitric Oxide Synthase Expression in Tumor Vasculature Is Correlated with Malignancy in Human Supratentorial Astrocytic Tumors Shinji Iwata, M.D., Kou Nakagawa, M.D., Hironobu Harada, M.D., Yoshihisa Oka, M.D., Yoshiaki Kumon, M.D., Saburo Sakaki, M.D. Department of Neurological Surgery, Ehime University School of Medicine, Ehime, )apan O BJEC T IV E: Endothelial nitric oxide synthase (eN O S) may play an important role in the regulation of tumor blood flow and vascular permeability. However, there have been no reports describing alterations of e N O S expression in relation to malignant progression in human astrocytic tumors. W e immunohistochemically studied the relationship between eN O S expression in tumor vasculature and malignancy in supratentorial astrocytic tumors. M ET H O D S: Tissue samples were obtained from 12 patients with low-grade astrocytomas, 10 with anaplastic astrocytomas, and 17 with glioblastomas. Normal brain tissue samples were obtained from four patients with other brain diseases. Immunohistochemical staining was performed using the avidin-biotin complex method, with polyclonal anti-eNOS antibody, and the levels of eN O S expression in endothelial cells were evaluated as slight, moderate, or intense on the basis of eN O S immunoreactivity. The proliferative potential was assessed as the MIB-1 staining index for tumor cells. RESULTS: The expression of eN O S was slight in all specimens of normal brain tissue, slight in 7 and moderate in 5 specimens of low-grade astrocytoma, slight in 2, moderate in 6, and intense in 2 specimens of anaplastic astrocytoma, and moderate in 5 and intense in 12 specimens of glioblastoma. The MIB-1 staining index (mean ± standard deviation) was 0.2 ± 0 .2 % for normal specimens, 1.8 ± 0 .6 % for low-grade astrocytomas, 9.6 ± 6 .9 % for anaplastic astrocytomas, and 18.5 ± 7 .7 % for glioblastomas. The MIB-1 staining indices for slight, moderate, and...
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Publisher
Congress of Neurological Surgeons
Copyright
© Published by Oxford University Press.
ISSN
0148-396X
eISSN
1524-4040
D.O.I.
10.1097/00006123-199907000-00006
Publisher site
See Article on Publisher Site

Abstract

AbstractOBJECTIVE:Endothelial nitric oxide synthase (eNOS) may play an important role in the regulation of tumor blood flow and vascular permeability. However, there have been no reports describing alterations of eNOS expression in relation to malignant progression in human astrocytic tumors. We immunohistochemically studied the relationship between eNOS expression in tumor vasculature and malignancy in supratentorial astrocytic tumorsMETHODS:Tissue samples were obtained from 12 patients with low-grade astrocytomas, 10 with anaplastic astrocytomas, and 17 with glioblastomas. Normal brain tissue samples were obtained from four patients with other brain diseases. Immunohistochemical staining was performed using the avidin-biotin complex method, with polyclonal anti-eNOS antibody, and the levels of eNOS expression in endothelial cells were evaluated as slight, moderate, or intense on the basis of eNOS immunoreactivity. The proliferative potential was assessed as the MIB-1 staining index for tumor cells.RESULTS:The expression of eNOS was slight in all specimens of normal brain tissue, slight in 7 and moderate in 5 specimens of low-grade astrocytoma, slight in 2, moderate in 6, and intense in 2 specimens of anaplastic astrocytoma, and moderate in 5 and intense in 12 specimens of glioblastoma. The MIB-1 staining index (mean ± standard deviation) was 0.2 ± 0.2% for normal specimens, 1.8 ± 0.6% for low-grade astrocytomas, 9.6 ± 6.9% for anaplastic astrocytomas, and 18.5 ± 7.7% for glioblastomas. The MIB-1 staining indices for slight, moderate, and intense eNOS expression were 2.0 ± 2.3%, 10.8 ± 9.8%, and 16.9 ± 7.7%, respectivelyCONCLUSION:Expression of eNOS in tumor vessels was significantly correlated with histological grade and proliferative potential. These findings suggest that astrocytic tumor vessels possess higher activity for nitric oxide production than do normal vessels.

Journal

NeurosurgeryOxford University Press

Published: Jul 1, 1999

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