DNA methylation as a marker for prenatal smoke exposure in adults

DNA methylation as a marker for prenatal smoke exposure in adults BackgroundPrenatal smoke exposure is known to be robustly associated with DNA methylation among offspring in early life, but whether the association persists into adulthood is unclear. This study aimed to investigate the long-term effect of maternal smoke exposure on DNA methylation in 754 women (mean age 30 years); to replicate findings in the same women 18 years later and in a cohort of 230 men (mean age 53 years); and to assess the extent to which a methylation score could predict prenatal smoke exposure.MethodsWe first carried out an epigenome-wide association analysis for prenatal smoke exposure and performed replication analyses for the top CpG sites in the other samples. We derived a DNA methylation score based on previously identified CpG sites and generated receiver operating characteristic (ROC) curves to assess the performance of these scores as predictors of prenatal smoke exposure.ResultsWe observed associations at 15 CpG sites in 11 gene regions: MYO1G, FRMD4A, CYP1A1, CNTNAP2, ARL4C, AHRR, TIFAB, MDM4, AX748264, DRD1, FTO (false discovery rate <5%). Most of these associations were specific to exposure during pregnancy, were present 18 years later and were replicated in a cohort of men. A DNA methylation score could predict prenatal smoke exposure (30 years previously) with an area under the curve of 0.72 (95% confidence interval 0.69, 0.76).ConclusionsThe results of this study provide robust evidence that maternal smoking in pregnancy is associated with changes in DNA methylation that persist in the exposed offspring for many years after prenatal exposure. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png International Journal of Epidemiology Oxford University Press

DNA methylation as a marker for prenatal smoke exposure in adults

11 pages
Read Article

Loading next page...
 
/lp/ou_press/dna-methylation-as-a-marker-for-prenatal-smoke-exposure-in-adults-dr9m94CkbW
Publisher
Oxford University Press
Copyright
© The Author(s) 2018. Published by Oxford University Press on behalf of the International Epidemiological Association.
ISSN
0300-5771
eISSN
1464-3685
DOI
10.1093/ije/dyy091
Publisher site
See Article on Publisher Site

Abstract

BackgroundPrenatal smoke exposure is known to be robustly associated with DNA methylation among offspring in early life, but whether the association persists into adulthood is unclear. This study aimed to investigate the long-term effect of maternal smoke exposure on DNA methylation in 754 women (mean age 30 years); to replicate findings in the same women 18 years later and in a cohort of 230 men (mean age 53 years); and to assess the extent to which a methylation score could predict prenatal smoke exposure.MethodsWe first carried out an epigenome-wide association analysis for prenatal smoke exposure and performed replication analyses for the top CpG sites in the other samples. We derived a DNA methylation score based on previously identified CpG sites and generated receiver operating characteristic (ROC) curves to assess the performance of these scores as predictors of prenatal smoke exposure.ResultsWe observed associations at 15 CpG sites in 11 gene regions: MYO1G, FRMD4A, CYP1A1, CNTNAP2, ARL4C, AHRR, TIFAB, MDM4, AX748264, DRD1, FTO (false discovery rate <5%). Most of these associations were specific to exposure during pregnancy, were present 18 years later and were replicated in a cohort of men. A DNA methylation score could predict prenatal smoke exposure (30 years previously) with an area under the curve of 0.72 (95% confidence interval 0.69, 0.76).ConclusionsThe results of this study provide robust evidence that maternal smoking in pregnancy is associated with changes in DNA methylation that persist in the exposed offspring for many years after prenatal exposure.

Journal

International Journal of Epidemiology – Oxford University Press

Published: Aug 1, 2018

References

  • What changes in DNA methylation take place in individuals exposed to maternal smoking in utero?
  • Contrasting the effects of intra-uterine smoking and one-carbon micronutrient exposures on offspring DNA methylation
  • DNA methylation in newborns and maternal smoking in pregnancy: genome-wide consortium meta-analysis
  • Prenatal exposure to maternal smoking and offspring DNA methylation across the lifecourse: findings from the Avon Longitudinal Study of Parents and Children (ALSPAC)
  • From stem cells to the law courts: DNA methylation, the forensic epigenome and the possibility of a biosocial archive
  • Prenatal exposure to maternal cigarette smoking and DNA methylation: epigenome-wide association in a discovery sample of adolescents and replication in an independent cohort at birth through 17 years of age
  • DNA methylation alterations in response to prenatal exposure of maternal cigarette smoking: a persistent epigenetic impact on health from maternal lifestyle?
  • Prenatal tobacco smoke exposure affects global and gene-specific DNA methylation
  • Maternal smoking during pregnancy is associated with epigenetic modifications of the brain-derived neurotrophic factor-6 exon in adolescent offspring
  • Presence of an epigenetic signature of prenatal cigarette smoke exposure in childhood
  • Prenatal tobacco smoke exposure is associated with childhood DNA CpG methylation
  • Maternal smoking during pregnancy and DNA-methylation in children at age 5.5 years: epigenome-wide-analysis in the European Childhood Obesity Project (CHOP)-Study
  • Environment-induced epigenetic reprogramming in genomic regulatory elements in smoking mothers and their children
  • Genomic DNA methylation among women in a multiethnic New York City birth cohort
  • Prenatal smoke exposure and genomic DNA methylation in a multiethnic birth cohort
  • Lymphoblastoid cell lines reveal associations of adult DNA methylation with childhood and current adversity that are distinct from whole blood associations
  • Maternal cigarette smoking during pregnancy and offspring DNA methylation in midlife
  • Cohort profile: the ‘children of the 90s’—the index offspring of the Avon Longitudinal Study of Parents and Children
  • Cohort profile: the Avon Longitudinal Study of Parents and Children: ALSPAC mothers cohort
  • Do grandmaternal smoking patterns influence the etiology of childhood asthma?
  • Data resource profile: Accessible Resource for Integrated Epigenomic Studies (ARIES)
  • Evaluation of the Infinium Methylation 450K technology
  • Negative controls a tool for detecting confounding and bias in observational studies
  • Negative control exposures in epidemiologic studies
  • Approaches for drawing causal inferences from epidemiological birth cohorts: a review
  • Differences in smoking associated DNA methylation patterns in South Asians and Europeans
  • Epigenetic signatures of cigarette smoking
  • DNA methylation mediates the effect of maternal smoking during pregnancy on birthweight of the offspring
  • Identification of DNA methylation changes in newborns related to maternal smoking during pregnancy
  • Life course epidemiology
  • Tobacco smoking leads to extensive genome-wide changes in DNA methylation
  • DNA methylation score as a biomarker in newborns for sustained maternal smoking during pregnancy
  • Maternal smoking during pregnancy and offspring smoking initiation: assessing the role of intrauterine exposure
  • Mediation analysis in epidemiology: methods, interpretation and bias
  • The impact of residual and unmeasured confounding in epidemiologic studies: a simulation study
  • An evaluation of methods correcting for cell-type heterogeneity in DNA methylation studies
  • DNA methylation arrays as surrogate measures of cell mixture distribution
  • Accounting for cellular heterogeneity is critical in epigenome-wide association studies
  • Epigenetic epidemiology of the developmental origins hypothesis
  • Challenges and novel approaches for investigating molecular mediation
  • Two-step epigenetic Mendelian randomization: a strategy for establishing the causal role of epigenetic processes in pathways to disease
  • Mendelian randomization: genetic anchors for causal inference in epidemiological studies
  • Epigenome-wide association studies and the interpretation of disease -Omics

You’re reading a free preview. Subscribe to read the entire article.

Try 2 weeks free now

DeepDyve is your
personal research library

It’s your single place to instantly
discover and read the research
that matters to you.

Enjoy affordable access to
over 18 million articles from more than
15,000 peer-reviewed journals.

All for just $49/month

Explore the DeepDyve Library

or browse the journals available

Search

Query the DeepDyve database, plus search all of PubMed and Google Scholar seamlessly

Organize

Save any article or search result from DeepDyve, PubMed, and Google Scholar... all in one place.

Access

Get unlimited, online access to over 18 million full-text articles from more than 15,000 scientific journals.

Your journals are on DeepDyve

Read from thousands of the leading scholarly journals from SpringerNature, Elsevier, Wiley-Blackwell, Oxford University Press and more.

All the latest content is available, no embargo periods.

See the journals in your area

DeepDyve

Freelancer

DeepDyve

Pro

Price

FREE

$49/month
$360/year

Save searches from
Google Scholar,
PubMed

Create folders to
organize your research

Export folders, citations

Read DeepDyve articles

Abstract access only

Unlimited access to over
18 million full-text articles

Print

20 pages / month

PDF Discount

20% off

Sign up
for free
Start 14 day
Free Trial