Abstract Drosophila suzukii (Matsumura) (Diptera: Drosophilidae) is a devastating global pest of berry crops and cherries. Little is understood about its biology during the winter in northern temperate regions, including potential resources that it may utilize during this period. In this study, olfactory and behavioral responses of female D. suzukii to six volatiles (methionol, acetic acid, linalool, bornyl acetate, isoamyl acetate, and geosmin) were evaluated separately for electroantennogram (EAG) and behavioral assays between summer and winter morphs. Results of EAG indicated that isoamyl acetate, acetic acid, and geosmin elicited significantly higher olfactory responses from the antennae of female summer morph D. suzukii compared with those of female winter morph D. suzukii. Winter morph D. suzukii showed reduced antennal response to the volatiles overall. Geosmin and bornyl acetate elicited significantly different behavioral responses from the two morphs in no-choice laboratory behavioral assays. T-maze behavioral assays with geosmin further revealed that summer morphs had a significant aversion, while winter morphs showed no significant aversion to geosmin. Overall, we demonstrate that responses of the two seasonally induced morphs to environmental stimuli are different, and future studies are justified to further understand how these physiological and behavioral differences may contribute to improved pest management of D. suzukii. spotted wing drosophila, electroantennogram, volatile, winter morph First discovered on mainland United States in 2008, Drosophila suzukii (Matsumura) (Diptera: Drosophilidae) has rapidly spread to become a devastating global pest of berry and cherry crops in the Americas and Europe (Walsh et al. 2011, Cini et al. 2012, Deprá et al. 2014, Asplen et al. 2015). Unlike other species of Drosophila, female D. suzukii have a large, serrated ovipositor that enables them to penetrate the skin of undamaged and ripening fruit and lay eggs inside (Walsh et al. 2011, Atalla et al. 2014). The feeding larvae degrade the fruit, causing significant crop losses (Bolda et al. 2010, Farnsworth et al. 2017). Current management programs rely primarily on regular applications of conventional broad-spectrum insecticides (Van Timmeren and Isaacs 2013, Haye et al. 2016, Diepenbrock et al. 2017), which are not only unsustainable, but also have negative impacts on beneficial arthropods and disrupt integrated pest management programs (Desneux et al. 2007, Biondi et al. 2012). Moreover, little is understood about the seasonal biology and behavior of D. suzukii, particularly during cold periods (Asplen et al. 2015), knowledge that is necessary to effectively implement control strategies. To adapt and survive in certain temperate climates, insects have evolved a number of physiological mechanisms for coping with the effects of low temperature, such as reproductive diapause, cold hardiness, and improved tolerance to environmental stressors such as extreme cold (Kimura 1988, Hoffmann et al. 2003, Teets and Denlinger 2013). Several species of Drosophila overwinter in cold climates as adults under leaf litter where they are protected from extreme cold temperatures by a layer of snow (Hoffmann et al. 2003, Stephens et al. 2015). Their overwintering strategy may include reproductive diapause, limiting their reproduction to favorable conditions, and directing their resources to winter survival (Hoffmann et al. 2003, Wallingford and Loeb 2016). Additionally, environmental signals such as shorter day lengths and decreasing temperatures can trigger seasonal adaptations such as rapid cold-hardening or gradual acclimation experienced during development (Lee et al. 1987, Teets and Denlinger 2013). Developmentally acclimated flies would be the most prepared to survive the starvation and freeze stress associated with overwintering (Wallingford and Loeb 2016). Drosophila suzukii has a wide climatic range and is now found on nearly every continent (Calabria et al. 2012, Deprá et al. 2014, Asplen et al. 2015). Phenotypic plasticity in response to seasonal changes produces separate morphs of D. suzukii that survive in a range of temperatures and environmental conditions, including low or freezing temperatures (Stephens et al. 2015, Jakobs et al. 2015, Shearer et al. 2016). The winter morph has a darker pigmented body and longer wings than the summer morph (Stephens et al. 2015, Shearer et al. 2016). In the laboratory, this morph can be induced when larvae are subjected to colder temperatures and shorter day lengths (Shearer et al. 2016). Additionally, winter morphs of D. suzukii have various upregulated and downregulated genes compared with summer morphs of D. suzukii that are responsible for female diapause, synthesis of cryoprotectants and chitin, and metabolic processes, among others (Shearer et al. 2016, Wallingford and Loeb 2016). Our current knowledge of the winter morph of D. suzukii is limited to physiological changes and oviposition capacity (Stephens et al. 2015, Jakobs et al. 2015, Shearer et al. 2016, Wallingford and Loeb 2016). As temperatures in northern regions begin to decline in the late fall and winter months, captures of D. suzukii in traps decline until captures are extremely low or zero. After January, typically no winter morph D. suzukii are captured in traps, and the first summer morphs are not captured in traps in late May or June in Michigan and northern regions (D. Kirkpatrick, unpublished data). Understanding differences in response to cues between the two morphs could potentially lead to improved monitoring during the winter and early spring periods and inform studies of early season biology. While attractive volatiles for summer morphs of D. suzukii for use in trapping have been identified (Cha et al. 2012, 2013; Revadi et al. 2015), there is a lack of information regarding volatiles that might be important for overwintering behavior. To better understand the influence of volatiles between these morphs, we evaluated 1) antennal responses to various odorants using electroantennogram (EAG), 2) morphology and number of olfactory sensory neurons using a scanning electron microscope (SEM), and 3) behavioral responses in no-choice and T-maze bioassays to determine if there was a differential response to volatiles between summer morphs and winter morphs of D. suzukii. Differential response to odorants may help optimize traps that are morph-dependent, but also allow us to better understand the overwintering behavior of D. suzukii. Materials and Methods Drosophila suzukii Colonies Laboratory-reared female adult D. suzukii were used in all experiments. The laboratory colony was initially obtained from field-collected flies in 2015 and was maintained as summer morphs on D. suzukii solid food diet (Dalton et al., 2011) in 50-ml polystyrene vials (Genesee Scientific, San Diego, CA) and held in a growth chamber at 24°C, 45% R.H., and 16:8 (L:D) h photoperiod. The winter morph D. suzukii colony was reared from the summer morph colony. Thirty adult summer morphs were held in vials with diet in a growth chamber at 24°C for 3 d. The adult flies were then removed from the vials, and the vials containing eggs and young larvae were placed in another growth chamber at 10°C, 45% R.H. and 12:12 (L:D) h photoperiod (Shearer et al. 2016). Winter morph flies eclosed from pupae in these vials after an average of 42 d. All flies used in the experiments were 3–7 d old and sexually mature. Flies were lightly anesthetized with CO2 to facilitate handling, sorting by sex, and counting. Odorants Odorants were purchased from Sigma–Aldrich (St. Louis, MO) and were of the highest purity available (>95%). Odorants used for experiments were isoamyl acetate (W205508-SAMPLE-K), geosmin (G5908-1ML), methionol (318396-5G), linalool (51782-1ML), bornyl acetate (45855-1ML-F), and acetic acid (64-19-7). Paraffin oil (18512-1L) was used as a solvent for all chemicals except acetic acid, where deionized water (DI) water was used as a solvent. Test odorants isoamyl acetate, geosmin, methionol, linalool, and bornyl acetate were diluted in paraffin oil to obtain three concentrations (10–1, 10–2, and 10–3) and the control solution was paraffin oil alone. The test odorant acetic acid was diluted in DI water to obtain three concentrations (10–1, 10–2, and 10–3) and the control solution for this odorant was DI water alone. Acetic acid, methionol, and isoamyl acetate were selected for their known attraction to summer morph D. suzukii (Cha et al. 2012, Revadi et al. 2015). Both bornyl acetate (derived from pine) and linalool (derived from flowers) were evaluated because of their potential as a sugar source when fruit is not available (sap from pines in the fall, and nectar from flowers in the spring). Geosmin (derived from soil-borne bacteria) was selected because it had previously been evaluated for summer morphs of D. suzukii as a potential deterrent (Wallingford et al. 2016) and due to the potential for soil and leaf litter to be an overwintering site for D. suzukii. Electroantennogram Recordings Antennal receptivity of adult female D. suzukii to synthetic compounds was determined by EAG. The procedure for EAG is similar to that previously described for Drosophila melanogaster by Ayer and Carlson (1992). Flies were held during recording in a 200-µl pipette tip. The fly was inserted into the pipette tip using a mouth aspirator with the head of the fly toward the small end of the tip. The small end of the tip was trimmed such that the antennae and head emerged from the end of the tip, and a second cut was made to trim the pipette tip approximately 1 mm behind the fly. To prevent the fly from crawling out backward, clay was placed in the large end of the cut tip, so that it touched the posterior end of the fly. The fly was then placed on a microscope slide with the head next to a coverslip that was mounted on the microscope slide. The antennae were maneuvered, so that they lay flat on the stack of the coverslip. To stabilize one of the antennae further, a glass capillary tube was used to hold the second antennal segment in place. The third antennal segment was centered under a stereomicroscope (Nikon SMA645, Japan) that was mounted on a vibration isolation table. Reference and recording electrodes were glass capillaries (World Precision Instruments, 1B100F-4), pulled such that tips were ≤1 µm in diameter, and filled with Drosophila Ringer’s solution (in mM): NaCl 100, KCl 5, MgCl2 20, CaCl2 0.15, HEPES 5, Sucrose 115, Trehalose 5. The reference electrode was inserted into the contralateral eye. Using a stereomicroscope (Nikon SMA645, Japan), the recording electrode was then brought into contact with the posterior aspect of the third antennal segment and advanced until stable electrical contact was established. The electrodes made electrical contact with a high impedance amplifier (World Precision Instruments, DAM 50) via silver/silver-chloride wires. The output signal of the amplifier was fed into a computer via a digitizer (Axon CNS molecular devices, Digidata 1440A). The electrical signal was collected using Axoscope 10.4 software and measured and analyzed by using Clampfit 10.4 software. Odor stimuli were presented from Pasteur pipettes holding solutions of chemicals in paraffin oil or DI water on filter paper. An aliquot of 50 µl of solution was dropped on a 1.27-cm filter paper strip placed in the shaft of a Pasteur pipette. The tip of a Pasteur pipette was placed through a hole in a tube that carried a humidified air stream over the fly. Compressed air was controlled by a solenoid that created puffs of odorant through the pipette. A minimum of 10 females of each morph was used in these tests. Flies were starved for 2 h prior to use in experiments. All EAG experiments were performed from 10:00 to 14:00 h at 21–22°C and 45–60% R.H. Scanning Electron Microscope Photos Summer and winter morph flies were placed in a −80°C freezer for 5 min. After defrosting, the flies’ heads were cut off with a small syringe and then fixed at 4°C for 1–2 h in 4% glutaraldehyde buffered with 0.1 M sodium phosphate at pH 7.4. Following a brief rinse in the buffer, samples were dehydrated in an ethanol series (25%, 50%, 75%, 95%) for 45 min at each gradation. Samples were critical point dried in a critical point dryer (EM CPD300, Leica Microsystems, Vienna, Austria) using carbon dioxide as the transitional fluid. The heads were mounted on aluminum stubs with heads facing up using carbon tape (Ted Pella, Inc., Redding, CA) and were then coated with osmium (≈10 nm thickness) in a NEOC-AT osmium chemical vapor deposition coater (Meiwafosis Co., Ltd., Osaka, Japan). Samples were examined and photos were taken in a JEOL 6610LV (tungsten hairpin emitter) scanning electron microscope (JEOL Ltd., Tokyo, Japan). Using the 250× magnification SEM photos of the third antennal segment, the total antennal area was calculated by tracing the circumference of the antennae using ImageJ. The numbers of ab4 and ab6 small basiconic sensilla were counted (de Bruyne et al. 2001) within a 40- × 60-μm area (approximately where the recording electrode was placed for the EAG studies) on the anterior of region 4 in the third antennal segment using the 600× magnification photos (Fig. 1). The ab4 basiconic sensilla are responsible for detection of the geosmin compound (Stensmyr et al. 2012), but it is difficult to visually distinguish from the ab6 basiconic sensilla (Venkatesh and Singh 1984); therefore, all small basioconic sensilla were counted and considered together. Fig. 1. View largeDownload slide Scanning electron microscope (SEM) photographs of the third antennal segments of Drosophila suzukii. Photograph (A) shown at 250× and photograph (B) at 600× magnification. Photograph (B) shows the 40 × 60 μm area (white box) used to count the number of small basiconic sensilla on the third antennal segment of summer and winter morphs. Fig. 1. View largeDownload slide Scanning electron microscope (SEM) photographs of the third antennal segments of Drosophila suzukii. Photograph (A) shown at 250× and photograph (B) at 600× magnification. Photograph (B) shows the 40 × 60 μm area (white box) used to count the number of small basiconic sensilla on the third antennal segment of summer and winter morphs. No-choice Test Behavioral Bioassay No-choice tests were conducted to elucidate behavioral responses of D. suzukii to volatiles. For behavioral assays, 20 ml glass scintillation vials (RPI Corp., Mount Prospect, IL) with a 3-D printed (Ultimaker 3, Cambridge, MA) plastic lids having 1-cm diameter opening in a funnel shape to facilitate capture of the flies were used as traps. Furthermore, 3.79-liter glass jars covered with a piece of mesh material to facilitate airflow were used as bioassay arenas. The bioassay arenas were placed in a room with 45–60% R.H. and 22°C. A piece of filter paper moistened with water was placed at the bottom of each bioassay arena to provide humidity inside. A vial trap containing either 1 ml of solution (concentration 10–3) or the control was also placed at the bottom of the arena. A yeast and sugar solution (83% DI water, 14% sugar, 3% baker’s yeast) was used as a positive control, because it is routinely used as bait for trapping D. suzukii (Burrack et al. 2015). Twenty female flies were held in the assay room to acclimate to the conditions in a Petri dish with a moist piece of filter paper in the bottom and were starved for 2 h before each experiment. The Petri dishes were placed in the bottom of the glass arena with the lids were on, and with the mesh covering the top of the arena, the tops of the Petri dishes were removed to allow the flies to emerge from the Petri dishes on their own. Traps were assessed after 24 h by counting the number of flies caught in each of the vial traps. T-maze Behavioral Bioassay T-maze assays were used to further elucidate stimulatory or deterrent effects of geosmin on summer and winter morphs D. suzukii following Stensmyr et al. (2012) with some modifications. Thus, a T-maze was constructed as follows: two 1,000-µl pipette tips were cut 2.5 cm from the narrow end and 0.5 cm from top. They were inserted into the bottom of two 1.5-ml microcentrifuge tubes with 2 mm removed from the tapered end. These two assembling units were connected using 4 cm of tubing (Tygon, E-3603, Akron, OH). In the middle of the tubing, a small hole was made and a 1,000-µl pipette tip (cut 0.5 cm from the narrow end) was inserted into the tubing via the opening. A 0.8 × 3.2 mm piece of filter paper was inserted between the pipette tip and the tip of microcentrifuge tube, so flies in the tubing could not make direct contact with the filter paper. A solvent or test compound (5 µl) was applied to the filter paper after the assembly. After the compound was applied, 10 female flies were gently introduced into the long pipette tip, and the opening was closed with a piece of cotton in the end of the pipette tip to keep the flies inside. In both microcentrifuge tube lids, a small hole was made using a syringe needle to facilitate airflow to maintain a concentration gradient for each tested compound. Trials were run in a room with 45–60% R.H. and 22°C. The numbers of flies entering the trap were counted after 24 h. The response index (RI) was calculated following the equation of Stensmyr et al. (2012) and calculated as (O−C)/T, where O is the number of flies in the baited arm, C is the number of flies in the control arm, and T is the total number of flies used in the trial. The resulting index ranges from −1 (complete avoidance) to 1 (complete attraction) (Stensmyr et al. 2012). Statistical Analyses EAG response data were normalized with response subtracted from the respective control solution and analyzed using a two-way analysis of variance. The antennal area and number of small basiconic sensilla satisfied normality assumptions using a Levene’s test and were compared using analysis of variance. Data from both behavioral experiments satisfied assumptions of normality using a Levene’s test and were analyzed using a two-way analysis of variance. Data from the no-choice tests were analyzed using a generalized linear mixed model with a Poisson distribution. The T-maze data were analyzed using the RI calculation with a generalized linear model. Tukey’s HSD was used for all post hoc comparisons. Data were analyzed using R (3.3.3, R Core Team, R Foundation for Statistical Computing, Vienna, Austria). Results Electroantennogram Recordings Using dose–response curves, we determined the appropriate concentration for each odorant tested to be 10–2, except for acetic acid, where a 10–1 concentration was also included. We found that summer morph response was elevated compared with winter morphs across four of the volatiles tested (Fig. 2). This was significantly different for geosmin (F1,18 = 4.9, P = 0.03), isoamyl acetate (F1,18 = 4.5, P = 0.04), and acetic acid 10–1 (F1,18 = 14.8, P = 0.001). Acetic acid 10–2 response was higher for summer morphs, but not significantly different (F1,18 = 4.1, P = 0.05). Likewise, methionol elicited a larger response in summer morphs, but not significantly so (F1,18 = 3.5, P = 0.07). There were no differences found between morphs for either bornyl acetate (F1,18 = 0.06, P = 0.8) or linalool (F1,18 = 0.04, P = 0.8). Fig. 2. View largeDownload slide Mean (±S.E.) summer morph (white bars) and winter morph (dark gray bars) D. suzukii response to volatiles in EAG assays. Asterisks indicate a significant difference between morphs, within each volatile tested (P < 0.05). Fig. 2. View largeDownload slide Mean (±S.E.) summer morph (white bars) and winter morph (dark gray bars) D. suzukii response to volatiles in EAG assays. Asterisks indicate a significant difference between morphs, within each volatile tested (P < 0.05). Scanning Electron Microscope Photos The area of the third antennal segment was similar between summer morphs (13.1 ± 0.9 mm) and winter morphs (13.2 ± 0.8 mm) (F1,7 = 0.001, P = 0.98). There were numerically more small basiconic sensilla found on summer morph antennae (8.6 ± 1.2) compared with winter morph antennae (6.2 ± 0.6), but these were statistically similar (F1,7 = 3.36, P = 0.1). No-choice Test Behavioral Bioassay Winter morph D. suzukii exhibited a significantly higher response to the volatiles geosmin (F1,14 = 9.36, P = 0.008) and bornyl acetate (F1,14 = 4.9, P = 0.04) compared with summer morphs in no-choice behavioral bioassays (Table 1). There was no significant difference between the morphs across any of the other tested volatiles. Table 1. Average percent response (±S.E.) and associated statistical values by winter and summer morph D. suzukii in a no-choice test behavioral bioassay to nine different volatiles Treatment Summer morph mean (%) Winter morph mean (%) F1,14 value P-value DI water 22.2 ± 5.4 27.2 ± 7.7 0.28 0.61 Paraffin oil 6.7 ± 1.7 15.6 ± 4.4 3.82 0.07 Yeast sugar 47.2 ± 7.5 38.3 ± 7.8 0.68 0.42 Geosmin 17.2 ± 3.2 35.6 ± 4.9 9.36 0.008 Bornyl acetate 10 ± 1.9 20.6 ± 4.5 4.90 0.04 Linalool 9.4 ± 3.7 7.2 ± 1.5 0.37 0.55 Acetic acid 17.2 ± 5.5 20.5 ± 6.6 0.17 0.68 Methionol 20.5 ± 4.1 10.5 ± 2.8 4.23 0.05 Isoamyl acetate 18.3 ± 3.7 17.2 ± 5.2 0.03 0.86 Treatment Summer morph mean (%) Winter morph mean (%) F1,14 value P-value DI water 22.2 ± 5.4 27.2 ± 7.7 0.28 0.61 Paraffin oil 6.7 ± 1.7 15.6 ± 4.4 3.82 0.07 Yeast sugar 47.2 ± 7.5 38.3 ± 7.8 0.68 0.42 Geosmin 17.2 ± 3.2 35.6 ± 4.9 9.36 0.008 Bornyl acetate 10 ± 1.9 20.6 ± 4.5 4.90 0.04 Linalool 9.4 ± 3.7 7.2 ± 1.5 0.37 0.55 Acetic acid 17.2 ± 5.5 20.5 ± 6.6 0.17 0.68 Methionol 20.5 ± 4.1 10.5 ± 2.8 4.23 0.05 Isoamyl acetate 18.3 ± 3.7 17.2 ± 5.2 0.03 0.86 Bold P-values indicate significantly different means between the two morphs. View Large Table 1. Average percent response (±S.E.) and associated statistical values by winter and summer morph D. suzukii in a no-choice test behavioral bioassay to nine different volatiles Treatment Summer morph mean (%) Winter morph mean (%) F1,14 value P-value DI water 22.2 ± 5.4 27.2 ± 7.7 0.28 0.61 Paraffin oil 6.7 ± 1.7 15.6 ± 4.4 3.82 0.07 Yeast sugar 47.2 ± 7.5 38.3 ± 7.8 0.68 0.42 Geosmin 17.2 ± 3.2 35.6 ± 4.9 9.36 0.008 Bornyl acetate 10 ± 1.9 20.6 ± 4.5 4.90 0.04 Linalool 9.4 ± 3.7 7.2 ± 1.5 0.37 0.55 Acetic acid 17.2 ± 5.5 20.5 ± 6.6 0.17 0.68 Methionol 20.5 ± 4.1 10.5 ± 2.8 4.23 0.05 Isoamyl acetate 18.3 ± 3.7 17.2 ± 5.2 0.03 0.86 Treatment Summer morph mean (%) Winter morph mean (%) F1,14 value P-value DI water 22.2 ± 5.4 27.2 ± 7.7 0.28 0.61 Paraffin oil 6.7 ± 1.7 15.6 ± 4.4 3.82 0.07 Yeast sugar 47.2 ± 7.5 38.3 ± 7.8 0.68 0.42 Geosmin 17.2 ± 3.2 35.6 ± 4.9 9.36 0.008 Bornyl acetate 10 ± 1.9 20.6 ± 4.5 4.90 0.04 Linalool 9.4 ± 3.7 7.2 ± 1.5 0.37 0.55 Acetic acid 17.2 ± 5.5 20.5 ± 6.6 0.17 0.68 Methionol 20.5 ± 4.1 10.5 ± 2.8 4.23 0.05 Isoamyl acetate 18.3 ± 3.7 17.2 ± 5.2 0.03 0.86 Bold P-values indicate significantly different means between the two morphs. View Large T-maze Behavioral Bioassay The summer morph flies exhibited a negative response and the winter morph flies showed a positive response to geosmin (Fig. 3). When the RI was compared with zero (no response), summer morphs had a significant avoidance to geosmin (F1,11 = 7.3, P = 0.01). However, response from winter morphs was not significantly different from zero (F1,11 = 0.01, P = 0.95). Fig. 3. View largeDownload slide Response index (RI) of summer morph (white bar) and winter morph (gray bar) D. suzukii (±S.E.) in a T-maze assay with a choice between geosmin (concentration 10–3) and paraffin oil control. Fig. 3. View largeDownload slide Response index (RI) of summer morph (white bar) and winter morph (gray bar) D. suzukii (±S.E.) in a T-maze assay with a choice between geosmin (concentration 10–3) and paraffin oil control. Discussion Electroantennography and the recording from receptor cells is a commonly used technique that has been previously used to explore D. suzukii antennal responses to individual volatile chemicals (Cha et al. 2012, Abraham et al. 2015, Revadi et al. 2015). This study reports the use of EAG for screening volatile chemicals that mediate attraction and drive behaviors between two different seasonally induced morphs of D. suzukii. EAG has also been used to previously compare several types of Drosophila mutants (Störtkuhl et al. 1999, Kain et al. 2008), but to our knowledge, this is the first time that differences in response to volatiles between seasonally induced insect morphs have been evaluated using any type of electrophysiological technique. In our EAG study, both summer and winter morphs showed an antennal response to all volatiles tested, suggesting that both morphs have the same type of olfactory sensory neurons and receptors present for these compounds. However, winter morphs had a consistently reduced response to most volatiles when compared with summer morphs. This could provide insights into behavioral strategies used between the two morphs for locating resources and successful overwintering sites. Winter morphs are thought to be quiescent during the winter, and a reduction in the capacity to respond to environmental stimuli and cold winter stress would likely benefit their survival during this period. EAG is excellent for quickly assessing the receptive range of an insect’s antenna and evaluates the sum of the electrical response of the activated olfactory sensory neurons on the entire antenna (Olsson and Hansson 2013). Despite being a commonly used electrophysiological technique for evaluating olfactory reception, EAG has potential shortcomings and is subject to change depending on the connection strength, insect vitality, and position of the electrode (Olsson and Hansson 2013). For a more quantitative measurement of the olfactory response, single-sensillum recording is an electrophysiological technique that can target specific receptor sites and could be used in the future to further evaluate the difference in response to volatiles between the two morphs of this species. Geosmin is a volatile compound produced by a select number of fungi, bacteria, and cyanobacteria (Stensmyr et al. 2012). It is typically recognized as a warning sign for the presence of toxic compounds for insects, and negatively affects behaviors such as attraction, feeding, and oviposition (Becher et al. 2010, Stensmyr et al. 2012). Geosmin is a known repellent for some species of Drosophila (Stensmyr et al. 2012), and has been previously evaluated as a repellent for D. suzukii summer morphs (Wallingford et al. 2016, Wallingford et al. 2017). We found that while the numbers of ab4 and ab6 small basiconic sensilla, where the ab4 and ab6 olfactory sensory neurons are located in other Drosophila (De Bruyne et al. 2001), were numerically lower on winter morph antennae compared with summer morphs, there was no statistical difference in the number of basiconic sensilla between morphs. This small reduction could be responsible for the change in EAG response, and further exploration is needed on the effect of the possible reduction of these basiconic sensilla. In our EAG study and the no-choice behavioral assay, there was a significant difference in response to geosmin between the two morphs. Thus, geosmin was the only volatile further evaluated in the T-maze behavioral bioassay to evaluate potential repellency to geosmin. In the T-maze assay, we found that there was a slight aversion to geosmin by summer morphs, and no significant response (aversion or attraction) to geosmin by winter morphs (Fig. 3). In a study by Wallingford et al. (2017), it was found that geosmin did not repel summer morphs and did not reduce the density of D. suzukii on treated fruit, but acted as an ovipositional deterrent. This is one possible explanation for why a higher aversion to geosmin was not seen from summer morphs in either type of behavioral bioassay, despite a strong relationship between other species of Drosophila and repellency, which is typically highly conserved among flies (Stensmyr et al. 2012). Other types of avoidance behaviors, such as avoidance to CO2, are not conserved in all Drosophila species and have been adapted to suit D. suzukii (Pham and Ray 2015). Low aversion to geosmin may also help explain why some summer morphs were found in vials containing geosmin in the no-choice assays, though still numerically less than winter morphs. Certain behaviors could be further adapted to suit winter morphs of D. suzukii and could offer a distinct evolutionary advantage for successful overwintering for winter morphs of D. suzukii by not avoiding volatiles that can potentially indicate overwintering sites. The landscape and fruit present when summer morphs are active and searching for suitable hosts are very different compared with the landscape for a winter morph D. suzukii searching for a suitable overwintering site. Further studies evaluating differential responses between the two morphs are warranted, particularly with geosmin. Our no-choice behavioral assay indicated a significantly higher response from winter morphs to bornyl acetate compared with summer morphs (Table 1). No difference with bornyl acetate was found in our EAG studies and in fact, very low response to this compound was observed with both morphs (Fig. 2). Interestingly, field studies have found high populations of D. suzukii in pine trees during the fall, but we are not currently aware of what drives this attraction. While there was a reduced response from winter morphs to many of the compounds tested using EAG, fewer differences were noted in our behavioral studies. We expect that D. suzukii behavior is highly plastic dependent on factors such as climate, temperature, humidity, resource availability, and fruit stage, among others. Therefore, we would expect to see greater difference between morphs when compared in their natural environmental settings. Other insects that produce a distinct seasonal morphs for winter survival also exhibit shifts in behavior, such as increased takeoff and flight capacity of winter morph cabbage whitefly, Aleyrodes proletella (Iheagwam 1977), longer wings for greater dispersal from orchards in the fall of winter morph pear psylla, Cacopsylla pyricola (Oldfield 1970), and migration from peaches and interrupted parthenogenesis of the overwintering green peach aphid, Myzus persicae (Blackman 1974). Additional studies could further elucidate preference for specific resource signals within and between morphs with two-choice or multi-choice experimental behavioral assays. Lower temperatures experienced by winter morphs in the field may also be important for observing representative behavior in behavioral bioassays. We also emphasize the need for further evaluation of the behavioral responses to these compounds in a field or semi-field study, relating spatial and temporal dependent factors to these morphs. Moreover, these morphs were lab-reared under static temperature and photoperiod conditions, and on a resource rich diet, which could have implications for the physiological and behavioral responses to environmental stimuli. There are important management implications for increased knowledge on the winter morph of D. suzukii, particularly because we expect that the surviving populations of this pest are quite low given low spring adult captures, similar to orchards with pear psylla populations where peak spring densities were often considerably lower than peak fall densities of the overwintering form (Horton et al. 1992). This provides a unique opportunity to exploit their winter or spring resources and further reduce the surviving population by targeting the winter morphs for control after the population bottleneck. Our findings indicate that seasonally induced morphs of D. suzukii have behavioral adaptations that likely help them to locate appropriate resources for successful overwintering in northern climates. Further defining the overwintering requirements for this pest will allow us to better understand their seasonal phenology and make appropriate management decisions. Acknowledgments We thank Peng Xu for his guidance with EAG studies and for his many hours spent teaching the proper techniques for successful EAG recordings. We also thank Amy Albin (Center for Advanced Microscopy, MSU) for help with the SEM photos of the flies and Megan Heil for colony maintenance, execution and data collection of laboratory assays. Funding was provided from the National Institute of Food and Agriculture, USDA Specialty Crops Research Initiative under Agreement No. 2015-51181-24252 and Michigan State University Project GREEEN grant GR17-023. References Cited Abraham , J. , A. Zhang , S. Angeli , S. Abubeker , C. Michel , Y. Feng , and C. Rodriguez-Saona . 2015 . Behavioral and antennal responses of Drosophila suzukii (Diptera: Drosophilidae) to volatiles from fruit extracts . Environ. Entomol . 44 : 356 – 367 . Google Scholar CrossRef Search ADS PubMed Asplen , M. K. , G. Anfora , A. Biondi , D. S. Choi , D. Chu , K. M. Daane , P. Gibert , A. P. Gutierrez , K. A. Hoelmer , W. D. Hutchison , et al. 2015 . Invasion biology of spotted wing Drosophila (Drosophila suzukii): a global perspective and future priorities . J. Pest Sci . 88 : 469 – 494 . Google Scholar CrossRef Search ADS Atallah , J. , L. Teixeira , R. Salazar , G. Zaragoza , and A. Kopp . 2014 . The making of a pest: the evolution of a fruit-penetrating ovipositor in Drosophila suzukii and related species . Proc. Royal Soc. London B: Bio. Sci . 281 : doi: 10.1098/rspb.2013.2840 . Ayer , R. K. , Jr , and J. Carlson . 1992 . Olfactory physiology in the Drosophila antenna and maxillary palp: acj6 distinguishes two classes of odorant pathways . J. Neurobiol . 23 : 965 – 982 . Google Scholar CrossRef Search ADS PubMed Becher , P. G. , M. Bengtsson , B. S. Hansson , and P. Witzgall . 2010 . Flying the fly: long-range flight behavior of Drosophila melanogaster to attractive odors . J. Chem. Ecol . 36 : 599 – 607 . Google Scholar CrossRef Search ADS PubMed Biondi , A. , V. Mommaerts , G. Smagghe , E. Viñuela , L. Zappalà , and N. Desneux . 2012 . The non-target impact of spinosyns on beneficial arthropods . Pest Manag. Sci . 68 : 1523 – 1536 . Google Scholar CrossRef Search ADS PubMed Blackman , R. L . 1974 . Life-cycle variation of Myzus persicae (Sulz.) (Hom., Aphididae) in different parts of the world, in relation to genotype and environment . Bull. Entomol. Res . 63 : 595 – 607 . Google Scholar CrossRef Search ADS Bolda , M. P. , R. E. Goodhue , and F. G. Zalom . 2010 . Spotted wing drosophila: potential economic impact of a newly established pest . J. Agr. Resour. Ec . 13 : 5 – 8 . de Bruyne , M. , K. Foster , and J. R. Carlson . 2001 . Odor coding in the Drosophila antenna . Neuron . 30 : 537 – 552 . Google Scholar CrossRef Search ADS PubMed Burrack , H. J. , M. Asplen , L. Bahder , J. Collins , F. A. Drummond , C. Guédot , R. Isaacs , D. Johnson , A. Blanton , J. C. Lee , et al. 2015 . Multistate comparison of attractants for monitoring drosophila suzukii (Diptera: Drosophilidae) in blueberries and caneberries . Environ. Entomol . 44 : 704 – 712 . Google Scholar CrossRef Search ADS PubMed Calabria , G. , J. Máca , G. Bächli , L. Serra , and M. Pascual . 2012 . First records of the potential pest species Drosophila suzukii (Diptera: Drosophilidae) in Europe . J. Appl. Entomol . 136 : 139 – 147 . Google Scholar CrossRef Search ADS Cha , D. H. , T. Adams , H. Rogg , and P. J. Landolt . 2012 . Identification and field evaluation of fermentation volatiles from wine and vinegar that mediate attraction of spotted wing Drosophila, Drosophila suzukii . J. Chem. Ecol . 38 : 1419 – 1431 . Google Scholar CrossRef Search ADS PubMed Cha , D. H. , S. P. Hesler , R. S. Cowles , H. Vogt , G. M. Loeb , and P. J. Landolt . 2013 . Comparison of a synthetic chemical lure and standard fermented baits for trapping Drosophila suzukii (Diptera: Drosophilidae) . Environ. Entomol . 42 : 1052 – 1060 . Google Scholar CrossRef Search ADS PubMed Cha , D. H. , T. Adams , C. T. Werle , B. J. Sampson , J. J. Adamczyk , Jr , H. Rogg , and P. J. Landolt . 2014 . A four-component synthetic attractant for Drosophila suzukii (Diptera: Drosophilidae) isolated from fermented bait headspace . Pest Manag. Sci . 70 : 324 – 331 . Google Scholar CrossRef Search ADS PubMed Cini , A. , C. Ioriatti , and G. Anfora . 2012 . A review of the invasion of Drosophila suzukii in Europe and a draft research agenda for integrated pest management . B. Insectol . 65 : 149 – 160 . Dalton , D. T. , V. M. Walton , P. W. Shearer , D. B. Walsh , J. Caprile , and R. Isaacs . 2011 . Laboratory survival of Drosophila suzukii under simulated winter conditions of the Pacific Northwest and seasonal field trapping in five primary regions of small and stone fruit production in the United States . Pest Manag. Sci . 67 : 1368 – 1374 . Google Scholar CrossRef Search ADS PubMed Deprá , M. , J. L. Poppe , H. J. Schmitz , D. C. De Toni , and V. L. Valente . 2014 . The first records of the invasive pest Drosophila suzukii in the South American continent . J. Pest Sci . 87 : 379 – 383 . Google Scholar CrossRef Search ADS Desneux , N. , A. Decourtye , and J. M. Delpuech . 2007 . The sublethal effects of pesticides on beneficial arthropods . Annu. Rev. Entomol . 52 : 81 – 106 . Google Scholar CrossRef Search ADS PubMed Diepenbrock , L. M. , J. A. Hardin , and H. J. Burrack . 2017 . Season-long programs for control of Drosophila suzukii in southeastern US blackberries . Crop Prot . 98 : 149 – 156 . Google Scholar CrossRef Search ADS Farnsworth , D. , K. A. Hamby , M. Bolda , R. E. Goodhue , J. C. Williams , and F. G. Zalom . 2017 . Economic analysis of revenue losses and control costs associated with the spotted wing drosophila, Drosophila suzukii (Matsumura), in the California raspberry industry . Pest Manag. Sci . 73 : 1083 – 1090 . Google Scholar CrossRef Search ADS PubMed Hauser , M . 2011 . A historic account of the invasion of Drosophila suzukii (Matsumura) (Diptera: Drosophilidae) in the continental United States, with remarks on their identification . Pest Manag. Sci . 67 : 1352 – 1357 . Google Scholar CrossRef Search ADS PubMed Haye , T. , P. Girod , A. G. S. Cuthbertson , X. G. Wang , K. M. Daane , K. A. Hoelmer , C. Baroffio , J. P. Zhang , and N. Desneux . 2016 . Current SWD IPM tactics and their practical implementation in fruit crops across different regions around the world . J. Pest Sci . 89 : 643 – 651 . Google Scholar CrossRef Search ADS Hoffmann , A. A. , M. Scott , L. Partridge , and R. Hallas . 2003 . Overwintering in Drosophila melanogaster: outdoor field cage experiments on clinal and laboratory selected populations help to elucidate traits under selection . J. Evol. Biol . 16 : 614 – 623 . Google Scholar CrossRef Search ADS PubMed Horton , D. R. , B. S. Higbee , T. R. Unruh , and P. H. Westigard . 1992 . Spatial characteristics and effects of fall density and weather on overwintering loss of pear psylla (Homoptera: Psyllidae) . Environ. Entomol . 21 : 1319 – 1332 . Google Scholar CrossRef Search ADS Iheagwam , E. U . 1977 . Comparative flight performance of the seasonal morphs of the cabbage whitefly, Aleyrodes brassicae (Wlk.), in the laboratory . Ecol. Entomol . 2 : 267 – 271 . Google Scholar CrossRef Search ADS Isaacs , R. , N. Hahn , B. Tritten , and C. Garcia . 2010 . Spotted wing drosophila: a new invasive pest of Michigan fruit crops . Michigan State University Extension E3140 , East Lansing . Jakobs , R. , T. D. Gariepy , and B. J. Sinclair . 2015 . Adult plasticity of cold tolerance in a continental-temperate population of Drosophila suzukii . J. Insect Physiol . 79 : 1 – 9 . Google Scholar CrossRef Search ADS PubMed Kain , P. , T. S. Chakraborty , S. Sundaram , O. Siddiqi , V. Rodrigues , and G. Hasan . 2008 . Reduced odor responses from antennal neurons of Gqα, phospholipase Cβ, and rdgA mutants in Drosophila support a role for a phospholipid intermediate in insect olfactory transduction . J. Neurosci . 28 : 4745 – 4755 . Google Scholar CrossRef Search ADS PubMed Kimura , M. T . 1988 . adaptations to temperate climates and evolution of overwintering strategies in the drosophila melanogaster species group . Evolution . 42 : 1288 – 1297 . Google Scholar CrossRef Search ADS PubMed Kimura , M. T . 2004 . Cold and heat tolerance of drosophilid flies with reference to their latitudinal distributions . Oecologia . 140 : 442 – 449 . Google Scholar CrossRef Search ADS PubMed Lee , R. E. , Jr , C. P. Chen , and D. L. Denlinger . 1987 . A rapid cold-hardening process in insects . Science . 238 : 1415 – 1417 . Google Scholar CrossRef Search ADS PubMed Oldfield , G. N . 1970 . Diapause and polymorphism in California populations of Psylla pyricola (Homoptera: Psyllidae) . Ann. Entomol. Soc. Am . 63 : 180 – 184 . Google Scholar CrossRef Search ADS Olsson , S. B. , and B. S. Hansson . 2013 . Electroantennogram and single sensillum recording in insect antennae . Methods Mol. Biol . 1068 : 157 – 177 . Google Scholar CrossRef Search ADS PubMed Pham , C. K. , and A. Ray . 2015 . Conservation of olfactory avoidance in Drosophila species and identification of repellents for Drosophila suzukii . Sci. Rep . 5 : 11527 . Google Scholar CrossRef Search ADS PubMed Revadi , S. , S. Vitagliano , M. V. Rossi Stacconi , S. Ramasamy , S. Mansourian , S. Carlin , U. Vrhovsek , P. G. Becher , V. Mazzoni , O. Rota‐Stabelli , et al. 2015 . Olfactory responses of Drosophila suzukii females to host plant volatiles . Physiol. Entomol . 40 : 54 – 64 . Google Scholar CrossRef Search ADS Shearer , P. W. , J. D. West , V. M. Walton , P. H. Brown , N. Svetec , and J. C. Chiu . 2016 . Seasonal cues induce phenotypic plasticity of Drosophila suzukii to enhance winter survival . BMC Ecol . 16 : 11 . Google Scholar CrossRef Search ADS PubMed Stensmyr , M. C. , H. K. Dweck , A. Farhan , I. Ibba , A. Strutz , L. Mukunda , J. Linz , V. Grabe , K. Steck , S. Lavista-Llanos , et al. 2012 . A conserved dedicated olfactory circuit for detecting harmful microbes in Drosophila . Cell . 151 : 1345 – 1357 . Google Scholar CrossRef Search ADS PubMed Stephens , A. R. , M. K. Asplen , W. D. Hutchison , and R. C. Venette . 2015 . Cold Hardiness of winter-acclimated Drosophila suzukii (Diptera: Drosophilidae) Adults . Environ. Entomol . 44 : 1619 – 1626 . Google Scholar CrossRef Search ADS PubMed Störtkuhl , K. F. , B. T. Hovemann , and J. R. Carlson . 1999 . Olfactory adaptation depends on the Trp Ca2+ channel in Drosophila . J. Neurosci . 19 : 4839 – 4846 . Google Scholar CrossRef Search ADS PubMed Teets , N. M. , and D. L. Denlinger . 2013 . Physiological mechanisms of seasonal and rapid cold‐hardening in insects . Physiol. Entomol . 38 : 105 – 116 . Google Scholar CrossRef Search ADS Van Timmeren , S. and R. Isaacs . 2013 . Control of spotted wing drosophila, Drosophila suzukii, by specific insecticides and by conventional and organic crop protection programs . Crop Prot . 54 : 126 – 133 . Google Scholar CrossRef Search ADS Venkatesh , S. and R. N. Singh . 1984 . Sensilla on the third antennal segment of Drosophila melanogaster Meigen (Diptera: Drosophilidae) . Internat. J. Insect Morphol. Embryol . 13 : 51 – 63 . Google Scholar CrossRef Search ADS Wallingford , A. K. , and G. M. Loeb . 2016 . Developmental acclimation of Drosophila suzukii (Diptera: Drosophilidae) and Its effect on Diapause and winter stress tolerance . Environ. Entomol . 45 : 1081 – 1089 . Google Scholar CrossRef Search ADS PubMed Wallingford , A. K. , S. P. Hesler , D. H. Cha , and G. M. Loeb . 2016 . Behavioral response of spotted-wing drosophila, Drosophila suzukii Matsumura, to aversive odors and a potential oviposition deterrent in the field . Pest Manag. Sci . 72 : 701 – 706 . Google Scholar CrossRef Search ADS PubMed Wallingford , A. K. , D. H. Cha , C. E. Linn , Jr , M. S. Wolfin , and G. M. Loeb . 2017 . Robust manipulations of pest insect behavior using repellents and practical application for integrated pest management . Environ. Entomol . 46 : 1041 – 1050 . Google Scholar CrossRef Search ADS PubMed Walsh , D. B. , M. P. Bolda , R. E. Goodhue , A. J. Dreves , J. Lee , D. J. Bruck , V. M. Walton , S. D. O’Neal , and F. G. Zalom . 2011 . Drosophila suzukii (Diptera: Drosophilidae): invasive pest of ripening soft fruit expanding its geographic range and damage potential . J. Integ. Pest Manag . 2 : 1 – 7 . Google Scholar CrossRef Search ADS © The Author(s) 2018. Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved. For permissions, please e-mail: email@example.com. This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/about_us/legal/notices)
Environmental Entomology – Oxford University Press
Published: Apr 14, 2018
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