Cloning, Expression and Characterization of UDP-Glucose Pyrophosphorylase from Shoots of Bambusa oldhamii

Cloning, Expression and Characterization of UDP-Glucose Pyrophosphorylase from Shoots of Bambusa... Abstract Carbohydrate biosynthesis for growing cell walls (wall extensibility) is presumed a crucial process to a rapidly growing plant. UDP-glucose pyrophosphorylase (UGPase) is a key enzyme involved in the cell wall synthesis. An UGPase cDNA was isolated from shoots of green bamboo ( Bambusa oldhamii ), which is a rapid-growing plant and valuable vegetable, by cDNA library screening. The complete sequence of the UGP cDNA was 1744 bp, contained a single open reading frame (ORF) of 1422 nucleotides which is translatable to a polypeptide composed of 473 amino acid residues including a putative transit peptide of 26 amino acid residues. The cloned UGPase of green bamboo shoot is closely related to that of grass family Poaceae of monocot plant by sequence analysis. The recombinant UGPase (rUGPase), which expressed in E. coli system, exhibited higher Km values for G1P and UTP than those of UGPase from bamboo. The reaction mechanism of rUGPase on substrate was shown a compulsory bi-bi mechanism. At various development stages, the UGPase in top portion of bamboo shoots exhibited the highest protein levels and activities than those in other portions. At least four different forms of UDP-glucose-synthesis catalyzing enzymes were existed in bamboo shoot. Bamboo ( Bambusa oldhamii ) , cDNA cloning, Gene expression, UDP-glucose, UDP-glucose pyrophosphorylase © The authors 2009. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png The Journal of Biochemistry Oxford University Press

Cloning, Expression and Characterization of UDP-Glucose Pyrophosphorylase from Shoots of Bambusa oldhamii

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Publisher
Oxford University Press
Copyright
© The authors 2009. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved.
ISSN
0021-924X
eISSN
1756-2651
D.O.I.
10.1093/jb/mvp084
Publisher site
See Article on Publisher Site

Abstract

Abstract Carbohydrate biosynthesis for growing cell walls (wall extensibility) is presumed a crucial process to a rapidly growing plant. UDP-glucose pyrophosphorylase (UGPase) is a key enzyme involved in the cell wall synthesis. An UGPase cDNA was isolated from shoots of green bamboo ( Bambusa oldhamii ), which is a rapid-growing plant and valuable vegetable, by cDNA library screening. The complete sequence of the UGP cDNA was 1744 bp, contained a single open reading frame (ORF) of 1422 nucleotides which is translatable to a polypeptide composed of 473 amino acid residues including a putative transit peptide of 26 amino acid residues. The cloned UGPase of green bamboo shoot is closely related to that of grass family Poaceae of monocot plant by sequence analysis. The recombinant UGPase (rUGPase), which expressed in E. coli system, exhibited higher Km values for G1P and UTP than those of UGPase from bamboo. The reaction mechanism of rUGPase on substrate was shown a compulsory bi-bi mechanism. At various development stages, the UGPase in top portion of bamboo shoots exhibited the highest protein levels and activities than those in other portions. At least four different forms of UDP-glucose-synthesis catalyzing enzymes were existed in bamboo shoot. Bamboo ( Bambusa oldhamii ) , cDNA cloning, Gene expression, UDP-glucose, UDP-glucose pyrophosphorylase © The authors 2009. Published by Oxford University Press on behalf of the Japanese Biochemical Society. All rights reserved.

Journal

The Journal of BiochemistryOxford University Press

Published: Jun 8, 2009

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