Arabidopsis subtilase SASP is involved in the regulation of ABA signaling and drought tolerance by interacting with OST1

Arabidopsis subtilase SASP is involved in the regulation of ABA signaling and drought tolerance... Abstract Arabidopsis Senescence-Associated Subtilisin Protease (SASP) was previously reported to participate in leaf senescence and development of inflorescence as well as silique. Here we describe a new role of SASP in the regulation of abscisic acid (ABA) signaling. SASP encodes a subtilase and its expression is considerably induced by darkness, ABA and ethylene treatments. sasp knockout mutants display obvious developmental phenotypes like early flowering and smaller leaves. Particularly, the sasp mutants exhibit enhanced ABA sensitivity during seed germination and seedling growth, heightened ABA-mediated leaf senescence, and increased ROS production. Importantly, the sasp mutants also show remarkably increased drought tolerance. In the sasp mutants, expression of six ABA signaling-related genes was either upregulated or downregulated following ABA treatments. Interaction assays demonstrated that SASP physically interacts with OPEN STOMATA 1 (OST1) at cell periphery. Co-expression of SASP and OST1 led to degradation of OST1, whereas the degradation of OST1 was impaired in sasp-1 protoplasts. Reactive oxygen species (ROS) attenuation assays revealed that in sasp-1 mutant guard cells, ROS attenuation rate markedly decreases. Altogether, our studies provide a demonstration that SASP plays an important role in regulating ABA signaling and drought tolerance through interaction with OST1. abscisic acid (ABA) signaling, Arabidopsis thaliana, drought tolerance, OPEN STOMATA 1 (OST1), reactive oxygen species (ROS), Senescence-Associated Subtilisin Protease (SASP) © The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/about_us/legal/notices) http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Experimental Botany Oxford University Press

Arabidopsis subtilase SASP is involved in the regulation of ABA signaling and drought tolerance by interacting with OST1

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Publisher
Oxford University Press
Copyright
© The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com
ISSN
0022-0957
eISSN
1460-2431
D.O.I.
10.1093/jxb/ery205
Publisher site
See Article on Publisher Site

Abstract

Abstract Arabidopsis Senescence-Associated Subtilisin Protease (SASP) was previously reported to participate in leaf senescence and development of inflorescence as well as silique. Here we describe a new role of SASP in the regulation of abscisic acid (ABA) signaling. SASP encodes a subtilase and its expression is considerably induced by darkness, ABA and ethylene treatments. sasp knockout mutants display obvious developmental phenotypes like early flowering and smaller leaves. Particularly, the sasp mutants exhibit enhanced ABA sensitivity during seed germination and seedling growth, heightened ABA-mediated leaf senescence, and increased ROS production. Importantly, the sasp mutants also show remarkably increased drought tolerance. In the sasp mutants, expression of six ABA signaling-related genes was either upregulated or downregulated following ABA treatments. Interaction assays demonstrated that SASP physically interacts with OPEN STOMATA 1 (OST1) at cell periphery. Co-expression of SASP and OST1 led to degradation of OST1, whereas the degradation of OST1 was impaired in sasp-1 protoplasts. Reactive oxygen species (ROS) attenuation assays revealed that in sasp-1 mutant guard cells, ROS attenuation rate markedly decreases. Altogether, our studies provide a demonstration that SASP plays an important role in regulating ABA signaling and drought tolerance through interaction with OST1. abscisic acid (ABA) signaling, Arabidopsis thaliana, drought tolerance, OPEN STOMATA 1 (OST1), reactive oxygen species (ROS), Senescence-Associated Subtilisin Protease (SASP) © The Author(s) 2018. Published by Oxford University Press on behalf of the Society for Experimental Biology. All rights reserved. For permissions, please email: journals.permissions@oup.com This article is published and distributed under the terms of the Oxford University Press, Standard Journals Publication Model (https://academic.oup.com/journals/pages/about_us/legal/notices)

Journal

Journal of Experimental BotanyOxford University Press

Published: Jun 1, 2018

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