Abstract from the Chinese Journal of Hypertension

Abstract from the Chinese Journal of Hypertension ABSTR AC TS FROM THE CHINESE JOURNAL OF HYPERTENSION The Effects of Renal Sympathetic Denervation on Hypothalamic Mitogen-Activated Protein Kinase and NF-κB in Spontaneously Hypertensive Rats 1 1 1 1 1 1 Li Wang, Cheng-zhi Lu, Yi-jie Ma, Meng-ping Xv, Zhu-qing Li, and Chao Li Department of cardiology, Tianjin First Center Hospital, Tianjin, China. Correspondence: Cheng-zhi Lu (lucz8@126.com). Objective: To study the effects of renal sympathetic denervation (RDN) on blood pressure, hypothalamic mitogen-activated protein kinase (MAPK), and nuclear factor-kappa B (NF-κB) in spontaneously hypertensive rats (SHR). Methods: Thirty-six SHR were randomly divided into baseline, RDN, and sham-operated groups ( n = 12 rats per group). Age-matched Wistar-Kyoto (WKY) rats served controls (n  =  12). At 12 weeks of age, rats underwent RDN or sham sur- gery and were killed aer 1 o ft r 6 weeks aer s ft urgery. Blood pressure, plasma levels of neuron-specific enolase (NSE), and p-p38MAPK, p65 NF-κB in the hypothalamus were assessed. Results: At 1 to 5 weeks aer ft RDN, systolic and diastolic blood pressures were significantly decreased. Compared with sham surgery at 1 week and SHR baseline, blood pressure was significantly decreased at 1 week aer RD ft N (all P < 0.05). At 6 weeks aer s ft urgery, there were no statistical differences in the blood pressure among RDN, sham surgery and SHR baseline groups (all P > 0.05). Plasma NSE levels were increased in SHR baseline compared to WKY and were further increased 1 week aer ft RDN (P < 0.05). Phosphorylation of p38 MAPK and p65 NF-κB proteins levels in the hypothalamus were increased in SHR groups compared to WKY controls and were decreased 1 week aer RD ft N compared to SHR baseline (all P < 0.05). Conclusion: RDN decreases blood pressure and hypothalamic p38 MAPK and NFκB levels in SHR. Arachidonic Acid Induces Human Podocyte Apoptosis 1 1 1 1 1 Yang-bin Pan, Shi-xiang Zhen, Hong-tin Ke, Guo-hua Ding, and Jian-xin Wan Department of Nephrology, First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, China. Correspondence: Jian-xin Wan (wanjx@263.com). Objective: To study the effects of arachidonic acid (AA) on cell apoptosis, p38 phosphorylation, and p53 levels in cultured human podocytes. Methods: Conditionally immortalized human podocytes were cultured in vitro. Differentiated human podocytes were −8 −5 exposed to AA at variable times (0.5–2.0 hours) and concentrations (10 –10 mol/l). Podocyte apoptosis was assessed by flow cytometry and Hoechst-33342 staining. Levels of p-p38 and p53 were analyzed by Western blotting. −8 −5 Results: Treatment with AA at 10 to 10 mol/l for 2 hours dose-dependently increased podocyte apoptosis as assessed −6 −5 by flow cytometry, along with increased levels of p-p38 and p53 at the higher doses (10 to 10 mol/l, P < 0.05). However, −8 −7 compared to controls, AA treatment at lower doses (10 and 10 mol/l) for 2 hours did not significantly increase podocyte −5 apoptosis or p53 levels. When podocytes were exposed to 10 mol/l AA for 0.5 hours, no significant changes were observed in podocyte apoptosis or p53 levels. Conclusion: AA dose-dependently induces cell apoptosis, and increases p-p38 and p53 levels in cultured human podocytes. 512 American Journal of Hypertension 31(4) April 2018 Downloaded from https://academic.oup.com/ajh/article-abstract/31/4/512/4925895 by Ed 'DeepDyve' Gillespie user on 16 March 2018 http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png American Journal of Hypertension Oxford University Press

Abstract from the Chinese Journal of Hypertension

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Publisher
Oxford University Press
Copyright
© American Journal of Hypertension, Ltd 2018. All rights reserved. For Permissions, please email: journals.permissions@oup.com
ISSN
0895-7061
eISSN
1941-7225
D.O.I.
10.1093/ajh/hpy016
Publisher site
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Abstract

ABSTR AC TS FROM THE CHINESE JOURNAL OF HYPERTENSION The Effects of Renal Sympathetic Denervation on Hypothalamic Mitogen-Activated Protein Kinase and NF-κB in Spontaneously Hypertensive Rats 1 1 1 1 1 1 Li Wang, Cheng-zhi Lu, Yi-jie Ma, Meng-ping Xv, Zhu-qing Li, and Chao Li Department of cardiology, Tianjin First Center Hospital, Tianjin, China. Correspondence: Cheng-zhi Lu (lucz8@126.com). Objective: To study the effects of renal sympathetic denervation (RDN) on blood pressure, hypothalamic mitogen-activated protein kinase (MAPK), and nuclear factor-kappa B (NF-κB) in spontaneously hypertensive rats (SHR). Methods: Thirty-six SHR were randomly divided into baseline, RDN, and sham-operated groups ( n = 12 rats per group). Age-matched Wistar-Kyoto (WKY) rats served controls (n  =  12). At 12 weeks of age, rats underwent RDN or sham sur- gery and were killed aer 1 o ft r 6 weeks aer s ft urgery. Blood pressure, plasma levels of neuron-specific enolase (NSE), and p-p38MAPK, p65 NF-κB in the hypothalamus were assessed. Results: At 1 to 5 weeks aer ft RDN, systolic and diastolic blood pressures were significantly decreased. Compared with sham surgery at 1 week and SHR baseline, blood pressure was significantly decreased at 1 week aer RD ft N (all P < 0.05). At 6 weeks aer s ft urgery, there were no statistical differences in the blood pressure among RDN, sham surgery and SHR baseline groups (all P > 0.05). Plasma NSE levels were increased in SHR baseline compared to WKY and were further increased 1 week aer ft RDN (P < 0.05). Phosphorylation of p38 MAPK and p65 NF-κB proteins levels in the hypothalamus were increased in SHR groups compared to WKY controls and were decreased 1 week aer RD ft N compared to SHR baseline (all P < 0.05). Conclusion: RDN decreases blood pressure and hypothalamic p38 MAPK and NFκB levels in SHR. Arachidonic Acid Induces Human Podocyte Apoptosis 1 1 1 1 1 Yang-bin Pan, Shi-xiang Zhen, Hong-tin Ke, Guo-hua Ding, and Jian-xin Wan Department of Nephrology, First Affiliated Hospital of Fujian Medical University, Fuzhou, Fujian, China. Correspondence: Jian-xin Wan (wanjx@263.com). Objective: To study the effects of arachidonic acid (AA) on cell apoptosis, p38 phosphorylation, and p53 levels in cultured human podocytes. Methods: Conditionally immortalized human podocytes were cultured in vitro. Differentiated human podocytes were −8 −5 exposed to AA at variable times (0.5–2.0 hours) and concentrations (10 –10 mol/l). Podocyte apoptosis was assessed by flow cytometry and Hoechst-33342 staining. Levels of p-p38 and p53 were analyzed by Western blotting. −8 −5 Results: Treatment with AA at 10 to 10 mol/l for 2 hours dose-dependently increased podocyte apoptosis as assessed −6 −5 by flow cytometry, along with increased levels of p-p38 and p53 at the higher doses (10 to 10 mol/l, P < 0.05). However, −8 −7 compared to controls, AA treatment at lower doses (10 and 10 mol/l) for 2 hours did not significantly increase podocyte −5 apoptosis or p53 levels. When podocytes were exposed to 10 mol/l AA for 0.5 hours, no significant changes were observed in podocyte apoptosis or p53 levels. Conclusion: AA dose-dependently induces cell apoptosis, and increases p-p38 and p53 levels in cultured human podocytes. 512 American Journal of Hypertension 31(4) April 2018 Downloaded from https://academic.oup.com/ajh/article-abstract/31/4/512/4925895 by Ed 'DeepDyve' Gillespie user on 16 March 2018

Journal

American Journal of HypertensionOxford University Press

Published: Apr 1, 2018

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