Abstract Over the last few years, RNA-seq has been used to study alterations in alternative splicing related to several diseases. Bioinformatics workflows used to perform these studies can be divided into two groups, those finding changes in the absolute isoform expression and those studying differential splicing. Many computational methods for transcriptomics analysis have been developed, evaluated and compared; however, there are not enough reports of systematic and objective assessment of processing pipelines as a whole. Moreover, comparative studies have been performed considering separately the changes in absolute or relative isoform expression levels. Consequently, no consensus exists about the best practices and appropriate workflows to analyse alternative and differential splicing. To assist the adequate pipeline choice, we present here a benchmarking of nine commonly used workflows to detect differential isoform expression and splicing. We evaluated the workflows performance over different experimental scenarios where changes in absolute and relative isoform expression occurred simultaneously. In addition, the effect of the number of isoforms per gene, and the magnitude of the expression change over pipeline performances were also evaluated. Our results suggest that workflow performance is influenced by the number of replicates per condition and the conditions heterogeneity. In general, workflows based on DESeq2, DEXSeq, Limma and NOISeq performed well over a wide range of transcriptomics experiments. In particular, we suggest the use of workflows based on Limma when high precision is required, and DESeq2 and DEXseq pipelines to prioritize sensitivity. When several replicates per condition are available, NOISeq and Limma pipelines are indicated. alternative splicing, differential expression, RNA-seq, analysis workflow Introduction In high eukaryotes, many genes can produce multiple transcripts through alternative splicing (AS), a post-transcriptional regulatory mechanism responsible for the functional complexity and protein diversity made from a small number of genes [1, 2]. Splicing patterns are constantly changing, allowing organisms to respond to modifications in their environment [3, 4]. For instance, more than 90% of human genes are naturally alternatively spliced and misregulations of AS causing changes in absolute or relative isoform expression have been related to several diseases, including cancer . Hence, the determination of changes in splicing patterns is an important issue in basic and applied biomedical research. Today, RNA-seq is the most widely used technique to analyse transcriptome expression dynamics, including AS . In the analysis of AS, two types of changes in isoform expression can be envisioned: differential isoform expression (DIE) and differential splicing (DS). DIE refers to a change in the absolute expression of an isoform, whereas DS is related to changes in isoform proportions . In both cases, the transcriptomic analysis is based on quantification at different levels (i.e. isoform, exon) than gene expression . Several works have been published comparing and evaluating isoform quantification methods using synthetic and/or real RNA-seq data [6–9]. Moreover, numerous differential expression (DE) analysis tools exist for the study of DIE and DS, generally in a separate way [10–13]. In general, specific methods have been developed for DS analysis while DE methods at the gene level have been applied to the study of DIE . Although those methods are well known in DE analysis at the gene level, their performances over isoform expression data have not been deeply evaluated. Complementarily, while some studies comparing methods to detect changes in AS have been published, they are mainly based on a descriptive characterization of method features [1, 7, 14–15]. Hence, a systematic evaluation of workflow performance is needed to further assist the choice of the appropriate set of tools for AS among a plethora of available methods. In this sense, the most complete reported work compares eight popular software tools using both simulated and real RNA-seq data in several scenarios . However, this work focuses only on DS changes without consideration of DIE, or DIE and DS occurring simultaneously, and only uses plant data. Thus, there is no clear consensus about the best practices or workflows that should be used or combined to obtain a comprehensive assessment of AS changes in human RNA-seq data involving both DIE and DS together. Here we present a systematic evaluation and comparison of nine pipelines for the detection of DIE and DS events. In particular, the evaluated DIE workflows were based on isoform expression profiles and used five of the most popular tools: Cuffdiff2 , and the R packages: DESeq2 , EBSeq , Limma  and NOISeq . On the other hand, the DS-evaluated pipelines were based on Cuffdiff2, and the SplicingCompass , DEXSeq  and Limma R packages. The study was performed using both real and synthetic RNA-seq data sets. The evaluation of workflows performance is based on simulated experiments where isoform expression profiles were modified and controlled to simulate AS changes based on a real human RNA-seq experiment. Several experimental scenarios, varying the number of genes simulated as differentially expressed, as well as, the number of replicates per conditions were simulated. For pipelines comparison, suitable performance measures were obtained. General and practical guidelines based on the number of replicates, sensitivity, precision and percentages of true positives are provided in order to aid scientists in the selection of the most appropriate workflows for their data and analysis goals. Methods Definition of expression changes at the isoform level Let us suppose that there are three experimental conditions, A, B and C, and a gene g having two isoforms, gI and gII, having the expression values listed in Table 1. The comparison of A and B conditions reveals changes in gI and gII absolute expression, without modifications in their proportions, which is an example of DIE. Note that DIE refers to absolute changes in isoform expression and hence DIE methods use count matrices at the transcript level. When conditions A and C are compared, significant changes in isoform proportions involving small changes in absolute expressions are present. This comparison reveals alterations in the AS mechanism in C with respect to A condition, a phenomenon known as DS. The changes in the proportion of the isoforms from the same gene are usually evaluated by measuring the changes in the gene’s exon usage. Table 1 Illustration of changes in absolute and relative isoform expression occurred across three experimental conditions. The comparison of conditions A and B reflects the occurrence of differential absolute expression, keeping relative isoform proportions. The comparison of conditions B and C reflects alterations in the AS mechanism causing significant changes in isoform proportions Gene Isoform Expression in A Expression in B Expression in C Abs Rel (%) Abs Rel (%) Abs Rel (%) g gI 10 66.67 20 66.67 20 80 gII 5 33.33 10 33.33 5 20 Gene Isoform Expression in A Expression in B Expression in C Abs Rel (%) Abs Rel (%) Abs Rel (%) g gI 10 66.67 20 66.67 20 80 gII 5 33.33 10 33.33 5 20 Abs, Absolute expression value; Rel, relative expression value. Table 1 Illustration of changes in absolute and relative isoform expression occurred across three experimental conditions. The comparison of conditions A and B reflects the occurrence of differential absolute expression, keeping relative isoform proportions. The comparison of conditions B and C reflects alterations in the AS mechanism causing significant changes in isoform proportions Gene Isoform Expression in A Expression in B Expression in C Abs Rel (%) Abs Rel (%) Abs Rel (%) g gI 10 66.67 20 66.67 20 80 gII 5 33.33 10 33.33 5 20 Gene Isoform Expression in A Expression in B Expression in C Abs Rel (%) Abs Rel (%) Abs Rel (%) g gI 10 66.67 20 66.67 20 80 gII 5 33.33 10 33.33 5 20 Abs, Absolute expression value; Rel, relative expression value. Table 2 Simulation groups and subgroups. Groups were defined according to the combination of changes in absolute and relative expression of gene isoforms between the two experimental conditions Group Fold-change at isoform expression Change in the proportion of the major isoform Simulation subgroup DE 2 No change DE-2 4 No change DE-4 DIE 2 No change DIE-2 3 No change DIE-3 4 No change DIE-4 5 No change DIE-5 DS No change 0–0.7 DS-0-0.7 No change 0.1–0.4 DS-0.1-0.4 No change 0.3–0.6 DS-0.3-0.6 No change 0.5–0.8 DS-0.5-0.8 DIEDS 0.5 0.8–0.5 DIEDS-0.5-0.8-0.5 2 0.8–0.3 DIEDS-2-0.8-0.3 2 0.8–0.5 DIEDS-2-0.8-0.5 4 0.8–0.3 DIEDS-4-0.8-0.3 4 0.8–0.5 DIEDS-4-0.8-0.5 Group Fold-change at isoform expression Change in the proportion of the major isoform Simulation subgroup DE 2 No change DE-2 4 No change DE-4 DIE 2 No change DIE-2 3 No change DIE-3 4 No change DIE-4 5 No change DIE-5 DS No change 0–0.7 DS-0-0.7 No change 0.1–0.4 DS-0.1-0.4 No change 0.3–0.6 DS-0.3-0.6 No change 0.5–0.8 DS-0.5-0.8 DIEDS 0.5 0.8–0.5 DIEDS-0.5-0.8-0.5 2 0.8–0.3 DIEDS-2-0.8-0.3 2 0.8–0.5 DIEDS-2-0.8-0.5 4 0.8–0.3 DIEDS-4-0.8-0.3 4 0.8–0.5 DIEDS-4-0.8-0.5 Table 2 Simulation groups and subgroups. Groups were defined according to the combination of changes in absolute and relative expression of gene isoforms between the two experimental conditions Group Fold-change at isoform expression Change in the proportion of the major isoform Simulation subgroup DE 2 No change DE-2 4 No change DE-4 DIE 2 No change DIE-2 3 No change DIE-3 4 No change DIE-4 5 No change DIE-5 DS No change 0–0.7 DS-0-0.7 No change 0.1–0.4 DS-0.1-0.4 No change 0.3–0.6 DS-0.3-0.6 No change 0.5–0.8 DS-0.5-0.8 DIEDS 0.5 0.8–0.5 DIEDS-0.5-0.8-0.5 2 0.8–0.3 DIEDS-2-0.8-0.3 2 0.8–0.5 DIEDS-2-0.8-0.5 4 0.8–0.3 DIEDS-4-0.8-0.3 4 0.8–0.5 DIEDS-4-0.8-0.5 Group Fold-change at isoform expression Change in the proportion of the major isoform Simulation subgroup DE 2 No change DE-2 4 No change DE-4 DIE 2 No change DIE-2 3 No change DIE-3 4 No change DIE-4 5 No change DIE-5 DS No change 0–0.7 DS-0-0.7 No change 0.1–0.4 DS-0.1-0.4 No change 0.3–0.6 DS-0.3-0.6 No change 0.5–0.8 DS-0.5-0.8 DIEDS 0.5 0.8–0.5 DIEDS-0.5-0.8-0.5 2 0.8–0.3 DIEDS-2-0.8-0.3 2 0.8–0.5 DIEDS-2-0.8-0.5 4 0.8–0.3 DIEDS-4-0.8-0.3 4 0.8–0.5 DIEDS-4-0.8-0.5 Workflows for DE analysis Seven commonly used methods for DE analysis based on different approaches were chosen to analyse DIE and DS. The selected methods were: EBSeq, DESeq2, NOISeq, SplicingCompass, Limma, DEXSeq and Cuffdiff2. Specific pipelines for them were designed (Figure 1). The evaluated workflows were called: Cufflinks, DESeq2, EBSeq, Limma and NOISeq, in the case of DIE analysis (solid arrows), and CufflinksDS, DEXSeq, LimmaDS and SplicingCompass, for DS study (dashed arrows). It is worth nothing, that only Cuffdiff2 and Limma DE tools are able to perform the analysis of both DIE and DS. Figure 1 View largeDownload slide Schema of the nine pipelines evaluated on this work. Five workflows to evaluate differential isoform expression (DIE, solid arrows) and four to analyse differential splicing (DS, dashed arrows) were included. Pipelines were designed following the author’s recommendations to evaluate case–control experiments. All the workflows take as input the sequencing reads and generate a list of isoforms (DIE methods) or genes (DS methods) with significant changes. Figure 1 View largeDownload slide Schema of the nine pipelines evaluated on this work. Five workflows to evaluate differential isoform expression (DIE, solid arrows) and four to analyse differential splicing (DS, dashed arrows) were included. Pipelines were designed following the author’s recommendations to evaluate case–control experiments. All the workflows take as input the sequencing reads and generate a list of isoforms (DIE methods) or genes (DS methods) with significant changes. DIE workflows This group of pipelines takes as input data isoform expression levels obtained by quantification methods based on probabilistic isoform resolution models. These models try to assign reads or fragments to the isoforms they came from. To do this, they model the uncertainty derived from multiple isoforms having overlapping sequences . In this work, RSEM  was used as a quantification tool to generate isoform count matrices from reads aligned against the human reference transcriptome using the Bowtie tool , as suggested by Teng et al. and Liu et al. [8, 16]. To evaluate DIE, four methods were used i.e. DESeq2, EBSeq, Limma and NOISeq, which are R packages that accept count data at isoform level. EBSeq and DESeq2 assume that the raw expression counts follow a negative binomial (NB) distribution, whereas Limma assumes that the logarithmic transformation of expression counts follows a normal distribution. To infer DE changes between experimental conditions, EBSeq uses a Bayesian hierarchical model , while DESeq2 combines empirical Bayes shrinkage with generalized linear model estimations to obtain model coefficients and then uses the Wald statistic . The voom transformation  applies a generalized least squares approach by modelling the mean–variance relationship with precision weights, allowing the use of the classical eBayes Limma method to detect the isoform expression changes . The three methods return a P-value adjusted to control the false discovery rate (FDR), used here to call differentially expressed isoforms. The NOISeqbio tool, from the NOISeq package, is a non-parametric and data-adaptive method that uses fold-changes and absolute expression differences between the experimental conditions to obtain one statistic per isoform. This method performs a permutation step to obtain the noise distribution, against which the isoform statistics will be compared . NOISeqbio returns for each isoform the probability of being differentially expressed (Pde) and the adjusted P-value is 1−Pde. DS workflows In the case of DS workflows, the analysis is performed over expression matrices at several levels obtained from alignments against the reference genome. The gapped aligner TopHat2  was used to do this mapping. The evaluated DS methods were Cuffdiff2 and three R packages: SplicingCompass, DEXSeq and Limma. The coverageBed tool  was used to obtain expression matrices for SplicingCompass, applying a union transcript model for each gene. With this information, SplicingCompass constructs vectors of exon and junction counts for each gene and sample, then calculates pairwise geometric angles between two samples and uses a t-test to compare geometric angles . DEXSeq is based on NB generalized linear models , like DESeq2. Count matrices at exon level for DEXSeq and Limma packages were obtained using the python script provided by the DEXSeq package, disabling the aggregate options, as suggested by Soneson etal. . DEXSeq and Limma models incorporate an interaction term between the condition and the exon identifier to evaluate changes in the proportion of that exon within a gene and between conditions. The initSigGenesFromResults (SplicingCompas), perGeneQValue (DEXSeq) and diffSplice (Limma) functions were used to compute adjusted P-values per gene. The C ++ Cufflinks2 program was used to calculate the isoform expression values as fragments per kilobase million (FPKM) from reads aligned to genome sequences [11, 25]. Then, Cuffdiff2 performed DE analysis at isoform and splicing levels, generating the output files of the two workflows: Cufflinks (DIE case) and CufflinksDS (DS case). In all workflows, the Ensembl hg19/GRCh37 (v.75) human reference was used and significant isoform/gene changes were identified using an adjusted p-value threshold of 0.05. The program versions, as well as all the scripts used in this study, are available in Supplementary Material, as well as in the project GitHub repository (https://github.com/gamerino/benchmarkingDiffExprAndSpl). RNA-seq data sets A replicated human prostate cancer RNA-seq data set (GSE22260; ) was used. This data set consists of 30 samples, 10 from normal tissue (control, condition-C) and 20 from prostate carcinoma (tumour, condition-T), sequenced using the Illumina GAII platform with a pair-end protocol. In particular, 10 T samples are matched with the C samples. In order to avoid subject correlation, these 10 samples were discarded. In addition, samples were tagged as outlier samples by our quality control pipeline  and discarded to finally keep 16 samples, 8 per condition, that were then used to evaluate pipelines on a real data set and to feed the simulator. The generated synthetic data sets corresponded to three possible experimental scenarios with balanced design (S1, S2 and S3) combining DIE and DS events. The S1 and S2 scenarios involved eight non-matched samples, four per each condition. In S1, 5% of total genes were simulated to have expression differences (DIE/DS); whereas, S2 had 10% of changing genes, incrementing the between-conditions heterogeneity. The S3 scenario considered the effect of a different number of replicates per condition, involving 16 samples, eight for each condition, with 10% of differentially expressed genes, the same as S2. A simulation procedure, shown in Figure 2, was designed to obtain raw sequencing reads for each subject with controlled DIE and DS. The simulation was based on the rsem-simulate-reads tool from RSEM, which takes a customized isoform expression profile and model parameters, computed from a real RNA-seq data set, to generate synthetic sequencing reads. The implemented procedure consisted of three steps. In step one, each real sample was aligned against the reference transcriptome and, isoform expression profiles together with RNA-seq model parameters were obtained. In the second step, the expression matrix was pre-processed to exclude low-expressed genes (zero counts in at least one replicate of C and T). Then, a set of well-expressed genes (20 counts per million in at least one replicate of C) were randomly chosen to simulate DIE/DS. The expression counts for the i-th isoform of the g-th gene from the k-th condition were modelled by a NB distribution, yigk ∼ NB(µigk, θigk). The sample mean (µigk) and shape (θigk) for the NB distribution were computed over condition C and taken as a reference to compute the simulated parameters for both C and T conditions, incorporating DIE/DS in the group of genes to be simulated as differentially expressed. In the third step, the isoform counts for each replicate were generated from NB distributions with the modified mean and shape parameters, obtaining the simulation expression profiles. Finally, transcripts per million for each sample were computed and used to call the rsem-simulate-reads function to generate simulated raw reads. In order to provide statistical power to evaluate workflows performance, the third step of the simulation pipeline was run 10 times to obtain replications of each scenario keeping the same differentially expressed genes and NB parameters. Figure 2 View largeDownload slide Simulation procedure designed to generate the RNA-seq data sets used for evaluation of workflows. The procedure was divided into three steps. The first step was run once for each sample and it started by aligning reads to the reference. Next, alignments were processed to obtain the real isoform expression profiles and RNA-seq model parameters, for each replicate. In the second step, the mean and shape NB parameters for each isoform in each experimental condition were calculated and modified to simulate expression changes. This step was run once for each scenario. The third step was run 10 times for each scenario and consisted in generating the simulated isoform expression profiles using a NB distribution with the modified parameters. Finally, the customized expression matrix and the RNA-seq model, estimated before, were used by RSEM to obtain the simulated sequencing reads for each sample. Figure 2 View largeDownload slide Simulation procedure designed to generate the RNA-seq data sets used for evaluation of workflows. The procedure was divided into three steps. The first step was run once for each sample and it started by aligning reads to the reference. Next, alignments were processed to obtain the real isoform expression profiles and RNA-seq model parameters, for each replicate. In the second step, the mean and shape NB parameters for each isoform in each experimental condition were calculated and modified to simulate expression changes. This step was run once for each scenario. The third step was run 10 times for each scenario and consisted in generating the simulated isoform expression profiles using a NB distribution with the modified parameters. Finally, the customized expression matrix and the RNA-seq model, estimated before, were used by RSEM to obtain the simulated sequencing reads for each sample. Since DIE and DS occur simultaneously, both cases were jointly simulated (see Table 2). The set of genes selected to be differentially expressed was divided into four subsets: DE, DIE, DS and DIEDS. For the DIE and DE groups, changes in the expression of all isoforms of the gene were simulated, without modification of isoform proportions. The DIE group included genes having more than one annotated isoform; whereas, the DE group involved genes having only one annotated transcript. For the DS group, changes in isoform proportion were simulated, without modifications of the overall gene expression. For each gene, the proportion of the most expressed isoform (major isoform) was controlled and the remaining proportions were equally distributed along their other expressed isoforms. Finally, for the DIEDS group, the simultaneous occurrence of DIE and DS was simulated. Even though the DS occurrence could derive in DIE, we would include this group where we control the occurence of DIE and DS. More detailed information about simulation groups, subgroups and the computation of simulated profiles can be found in the Supplementary Material. Performance evaluation Commonly used performance measures were computed to evaluate workflows results at 10 simulations from each scenario . The result of each workflow was either a list of significant differentially expressed isoforms (DI), or genes detected as alternative spliced (ASG). Each isoform or gene detected as differentially expressed was called positive (P), and was classified as true positive (TP) or false positive (FP) based on whether it was or not simulated as differentially expressed, respectively. Meanwhile, an isoform or gene detected as not differentially expressed (negative, N) was classified as true negative (TN) or false negative (FN) depending on whether it was or not simulated as not differentially expressed, correspondingly. In particular, for DIE workflows, those isoforms simulated either as DIE, DE, DIEDS or DS were considered as TPs, since DS could cause DIE; given that we did not know if it occurred or not, isoforms from genes with DS were not considered as FNs. Then, accuracy, sensitivity, precision and the F-score (harmonic mean between sensitivity and precision) were computed. The ability of a workflow to deal with FPs was characterized measuring the FP rate (FPR). In addition, the correlation between pipeline performance and isoform/gene expression and effective length was also evaluated. For this, isoform/gene groups were first defined according to their expression level and secondly, according to their effective length. Then, performance measures for each isoform/gene group were obtained. We also evaluated the effect of simulation subgroup, i.e. DIEDS-2-0.8-0.3, and the effect of the number of isoforms per gene. In the first case, isoforms and genes were clustered according to their simulation subgroup (Table 2) and the TP rate (TPR) was computed in order to determine if the magnitude of deregulation influenced the DI/ASG detection. In the other case, genes were grouped according to their number of annotated isoforms, i.e. 1, 2–4, 5–9 and >9 transcripts and TPRs per each of those groups were computed. Those numbers correspond to the 33, 66 and 99 percentiles, respectively, of the distribution of the number of isoforms per gene in humans. Results and discussion Nine workflows for DIE and DS analysis were compared in this study based on synthetic data, where the true status of each isoform or gene was controlled. Three experimental scenarios, S1, S2 and S3 were designed to evaluate the effect of the percentage of differential genes (S1 and S2) and the number of replicates per condition (S2 and S3). Concordance of DE results The concordance of DE results was evaluated looking at the number and percentage of detected DI/ASG (P and TP) in the 10 replicates run for each scenario. Results for DIE and DS workflows are shown in Figure 3 and summarized in Supplementary Tables S4 and S5. In the case of DIE pipelines, the EBSeq workflow detected the highest amount of DI (>8500) in the three tested scenarios, whereas the Cufflinks pipeline found the lowest values, three times lower than the EBSeq results. However, EBSeq had the lowest number of P found simultaneously in the 10 simulations (Figure 3A), indicating the poor concordance of this method in all three scenarios. On the other hand, DESeq2 and Limma showed a higher concordance of P (>17%, Figure 3A) and TP (> 30% Figure 3B) along simulations, especially for S2 and S3, showing that they were more robust than EBSeq. Comparing S1 and S2 scenarios, EBSeq and Cufflinks methods did not show differences in the percentage of TPs. On the contrary, DESeq2, Limma and NOISeq increased this percentage in S2 by approximately 5%. TP percentages were increased by 10% for EBSeq and DESeq2 and only 1% for Cufflinks and Limma from S2 to S3. Meanwhile, NOISeq was the only method that showed the highest percentage of TP detections in S2. The FP percentage (Figure 3C) was <5% for all the scenarios and pipelines, indicating the effectiveness of the simulation procedure. Figure 3 View largeDownload slide Concordance of workflows results in the 10 simulations performed in each scenario. Here, concordance was measured by means of the percentage of significant detections (A), true significant detections (B) and false significant detections (C) of differential isoforms/genes found in the 10 runs of each scenario. Each panel is divided into two facets, one for DIE workflows, detecting differentially expressed isoforms, and other for DS workflows, detecting differentially spliced genes. Figure 3 View largeDownload slide Concordance of workflows results in the 10 simulations performed in each scenario. Here, concordance was measured by means of the percentage of significant detections (A), true significant detections (B) and false significant detections (C) of differential isoforms/genes found in the 10 runs of each scenario. Each panel is divided into two facets, one for DIE workflows, detecting differentially expressed isoforms, and other for DS workflows, detecting differentially spliced genes. In the case of the DS workflows, CufflinksDS found the lowest average number of ASG (<303); whereas, the highest values were observed for DEXSeq (>423). The lowest and the highest percentage of P were found for SplicingCompass (<20%) and LimmaDS (>25%), respectively (Figure 3A). Moreover, SplicingCompass and CufflinksDS had a poor concordance of TP detection (Figure 3B). Interestingly, all workflows except CufflinksDS, increased the percentage of P and TP in S2 with respect to S1 and S3. In terms of FP, LimmaDS pipeline showed the highest values, near to 10% (Figure 3C). Overall performance results The overall performance measures for the evaluated workflows on the simulated scenarios are listed in Supplementary Table S6. All DIE and DS workflows achieved a high accuracy (>0.85) in all scenarios and hence, this measure was not further considered in our comparisons. Sensitivity, precision and F-score for DIE workflows are shown in Figure 4, panels A–C. In terms of sensitivity (Figure 4A), all DIE pipelines reached values <0.65; the highest values were exhibited for EBSeq (S1 and S3), NOISeq (S2) and DESeq2 (S3), and the lowest for Cufflinks. In terms of precision (Figure 4B), EBSeq and NOISeq (S1 and S2) had low performance; whereas, values >0.9 were achieved by Limma, Cufflinks (S2 and S3) and NOISeq (S3). It is worth mentioning that only Limma (S1) and NOISeq (S3) were able to control the imposed FDR, achieving precisions >0.95. When the three experimental scenarios were compared, an improvement from S1 to S3 was noted, except for NOISeq’s sensitivity and Limma’s precision, which showed opposite behaviours. In terms of the F-score, the best values (>0.7) were found for DESeq2, EBSeq, Limma (S3) and NOISeq (S1 and S2). Thus, DESeq2 and EBSeq workflows seem to be adequate to DIE analysis. However, if precision is preferred, Limma and NOISeq are recommended. Figure 4 View largeDownload slide Overall performance measures along 10 simulations performed in three experimental scenarios. A–C: DIE pipelines, D–F: DS workflows. Figure 4 View largeDownload slide Overall performance measures along 10 simulations performed in three experimental scenarios. A–C: DIE pipelines, D–F: DS workflows. The Figure 4D–F summarizes the performance results for DS workflows. In terms of sensitivity (Figure 4D), DEXSeq and LimmaDS had the best performance, achieving values >0.5 in nearly all scenarios; whereas CufflinksDS and SplicingCompass exhibited the poorest results. However, CufflinksDS showed the highest precision (>0.8), controlling also the FDR in S3 (Figure 4E). Although DEXSeq had the lowest precision (<0.75), this method together with LimmaDS, achieved higher F-score values in all cases (>0.55) and hence these pipelines are adequate to detect ASG with high sensitivity and precision. Particularly, LimmaDS had lower sensitivity than DEXSeq but, it reported ASG more precisely. The ability to deal with FP results was evaluated using the FPR (Supplementary Figure S1). In the case of DIE workflows (Supplementary Figure S1A–C), the lowest FPR was achieved by Cufflinks in all scenarios, whereas EBSeq, in the three scenarios, and NOISeq, in S1 and S2 had the highest FPR. Regarding DS pipelines (Supplementary Figure S1D–F), CufflinksDS showed the lowest FP values and DEXSeq the worst. In general, FPR values did not exceed 0.05, with higher values for S2 in comparison with S1. In the S3 all DIE pipelines, except Limma, had lower FPRs in respect to S2. Average performance measures were correlated with the isoform/gene expression level (Supplementary Figures S3, S4 and S5) and with isoform/gene effective length (Supplementary Figures S6, S7 and S8). It was found that both expression and length affected more the performance of DS workflows than of DIE pipelines. Moreover, the latter exhibited performance stability mainly for S2 and S3 scenarios. In particular, higher performance scores and less dependence on expression level and length were observed for both DS and DIE pipelines in S3. In general, sensitivity, precision and F-score were higher for those isoform/gene with higher expression values, and lower isoform/gene length increased except for Cufflinks and CufflinksDS. Based on the poor performances described above for Cufflinks, EBSeq, CufflinksDS and SplicingCompass, these methods were excluded from further analysis and only five pipelines were selected for subsequent evaluations: DESeq2, Limma and NOISeq, for DIE analysis, DEXSeq and LimmaDS for DS study. Effect of the number of isoforms Figure 5 illustrates the relationship between the TPRs and the number of isoforms per gene. Upper (lower) panels show the results for the DIE (DS) workflows and the three evaluated scenarios. For DIE workflows (Figure 5A–C), the percentage of TPs was higher for isoforms belonging to genes with only one annotated transcript (gene group ‘1’) and lower for those belonging to genes with more than nine isoforms (gene group ‘>9’), in all scenarios. For instance, in S2 (Figure 5B), all workflows achieved percentages >75% for isoforms from gene group ‘1’; while the percentage of TPs in gene group ‘>9’ was <50%. We suspected this behaviour was caused by lower expression values and complexity of isoform reconstruction process when the number of isoforms per gene increases. DESeq2 and NOISeq showed the highest and similar TPRs in S1 (Figure 5A) and S2, while DESeq2 and Limma performed best in S3 (Figure 5C). In general, all workflows performed better in S2 compared to S1 and in S3 compared to S2, except for NOISeq, that had poorer TPRs in S3. Figure 5 View largeDownload slide TPR of DIE and DS workflows as a function of the number of isoforms per gene. Panels A, B, C are for DIE workflows and D, E, F for DS workflows at S1, S2 and S3, respectively. Figure 5 View largeDownload slide TPR of DIE and DS workflows as a function of the number of isoforms per gene. Panels A, B, C are for DIE workflows and D, E, F for DS workflows at S1, S2 and S3, respectively. In the case of DS workflows (Figure 5, panels D, E and F), the observed TPRs were very similar in all scenarios and in all gene groups. The highest values were achieved by DEXSeq (>60%). Notably, LimmaDS showed TPRs >40% that was better than Limma performance in DIE analysis. The TPRs practically did not change between S1 and S2, whereas in S3, DEXSeq and LimmaDS increased the TPRs in all gene groups. The relationship between FP and the gene groups is illustrated in Supplementary Figure S2. In the case of DIE pipelines, FPs distribution along gene groups was different between scenarios and pipelines. DESeq2 and NOISeq showed similar behaviour along S1 and S2, with most of FP (>35%) for gene group “>9”. In addition, the number of FPs increased with the number of isoforms per gene, as expected. Meanwhile, most FPs for Limma were found in the gene group ‘2–4’. Nevertheless, Limma behaved similarly to DESeq2 and NOISeq in S2 and S3, respectively. For DS workflows, FPs distributions along gene groups and scenarios were similar. In general, FPs were near to 20%, 25% and 45% for gene groups ‘2–4’, ‘5–9’ and ‘>9’, respectively. It was observed that for both, DEXSeq and LimmaDS, FPs were more abundant when the number of isoforms per gene increased. Effect of the magnitude of DE Finally, the effect of the magnitude of DE in the ability of each workflow to detect changes was evaluated (Figure 6). As expected, all DIE pipelines (Figure 6, panels A, B and C) showed a higher TPR when the magnitude of the expression change was increased; however, differences were evident as a function of the simulation group (DE, DIE or DIEDS) and the simulation scenario. While all methods had high TPRs when single-gene isoforms were simulated with a fold-change of 4 in all scenarios, important differences were observed at fold-changes of 2, where Limma behaved poorly in scenarios S1 and S2 and NOISeq in scenario S3. Surprisingly, all methods had much lower TPRs in the DIE group compared to DE. For example, nearly-perfect TP detection was achieved with a 4 fold-change in DE cases, this value dropped to ∼50% when talking about DIE transcripts at the same fold-change. This could be explained by the fact that multi-transcripts genes generated both high- and low-expressed isoforms and all of those were analysed to compute DIE analysis and TPRs calculation. And, as it is known, low-expressed transcripts that are differentially expressed are more difficult to detect than those highly expressed. When a change in isoform proportions was included in the simulation group (DIEDS), TPRs were again affected. In scenario S1, all pipelines performed better at detecting true isoform changes when there was also an effect on the relative proportion of the isoform, while this was only the case for transcripts with a 4 fold-change in scenario S3 and was pipeline-dependent in scenario S2. In general, and in agreement with other analyses, Limma performance was comparatively worse at S1 and S2 and NOISeq at S3. Figure 6 View largeDownload slide TPR for DIE and DS workflows as a function of the simulation subgroup as described in Table 2. Panels A, B, C for DIE and D, E, F, for DS workflows at scenarios S1, S2 and S3, respectively. Figure 6 View largeDownload slide TPR for DIE and DS workflows as a function of the simulation subgroup as described in Table 2. Panels A, B, C for DIE and D, E, F, for DS workflows at scenarios S1, S2 and S3, respectively. Figure 7 View largeDownload slide Schema of workflows selection based on the research experiment. Each circle box contains the DIE (solid) and the DS (dashed) pipelines recommended for transcriptomic analysis. Figure 7 View largeDownload slide Schema of workflows selection based on the research experiment. Each circle box contains the DIE (solid) and the DS (dashed) pipelines recommended for transcriptomic analysis. For DS pipelines (Figure 6, panels D, E and F), results were more predictable. As a general rule, DEXSeq performed better than LimmaDS in all simulation groups and scenarios. In addition, higher TPRs were found when the magnitude of the DS was bigger. In the DIEDS group, good performance was basically associated with the magnitude of the splicing change (values >0.75 for a 0.8-0.3 difference) and to a much lesser extent to the magnitude of the total fold-change of the gene (similar results for 0.5, 2 and 4 global gene fold-changes and only slightly lower in the DS 0.5-0.8 subgroup, that is, zero global gene change). For genes with splicing and not total expression differences (DS), better TPRs were found when the major isoform had zero (DS-0-0.7) or low (DS-0.1-0.4) relative expression in one condition, although the biggest effect for an improved TPR was given by the magnitude of the differential splicing: the DS-0-0.7 subgroup had much higher TPRs than DS-0.1-0.4, DS-0.3-0.6 and DS-0.5-0.8. Both for DIEDS and DS groups, overall performances were better when more replicates were present (S3 versus S1 and S2). Evaluation of the real RNA-seq experiment The 16 real RNA-seq samples from the prostate experiments previously used for the simulation were then analysed with the nine evaluated workflows. The number of significant DI and genes reported by each pipeline and the overlap between them is summarized in Table 3 and graphically shown on Supplementary Figure S9. We found important differences in the number of detected transcripts/genes among different pipelines, which is in agreement with previous observations (Liu et al. ). The highest number of DI was detected by EBSeq (4600) whereas the lowest was achieved by NOISeq (11) followed by Cufflinks (204) and Limma (342). Meanwhile, DEXSeq found the highest number of ASG (1727) whereas CufflinksDS did not detect significant genes. These trends are consistent with our results on simulated data sets. Interestingly, most DESeq2, Limma and NOISeq calls were present in the EBSeq results, suggesting they capture a common data structure but with different levels of stringency (Table 3). On the contrary, Cufflinks had poor overlap with any of the other methods. On the other hand, DS pipelines were more discrepant. SplicingCompass overlapped only by 8% and 38%, while 80% of the LimmaDS calls were present in DEXSeq, and logically CufflinksDS had zero overlap with other pipelines. These results suggest that the choice of analysis method in DS is more critical than in the DIE analysis. Table 3 Overlap in differentially expressed isoforms and spliced genes in the nine evaluated workflows for the prostate cancer data set Type of detection Workflows DIE Cufflinks DESeq2 EBSeq Limma NOISeq Cufflinks 204 92 90 19 1 DESeq2 1572 1269 281 10 EBSeq 4600 324 11 Limma 342 10 NOISeq 11 DS CufflinksDS DEXSeq LimmaDS SplicingCompass CufflinksDS 0 0 0 0 DEXSeq 1727 82 35 LimmaDS 103 7 SplicingCompass 91 Type of detection Workflows DIE Cufflinks DESeq2 EBSeq Limma NOISeq Cufflinks 204 92 90 19 1 DESeq2 1572 1269 281 10 EBSeq 4600 324 11 Limma 342 10 NOISeq 11 DS CufflinksDS DEXSeq LimmaDS SplicingCompass CufflinksDS 0 0 0 0 DEXSeq 1727 82 35 LimmaDS 103 7 SplicingCompass 91 Table 3 Overlap in differentially expressed isoforms and spliced genes in the nine evaluated workflows for the prostate cancer data set Type of detection Workflows DIE Cufflinks DESeq2 EBSeq Limma NOISeq Cufflinks 204 92 90 19 1 DESeq2 1572 1269 281 10 EBSeq 4600 324 11 Limma 342 10 NOISeq 11 DS CufflinksDS DEXSeq LimmaDS SplicingCompass CufflinksDS 0 0 0 0 DEXSeq 1727 82 35 LimmaDS 103 7 SplicingCompass 91 Type of detection Workflows DIE Cufflinks DESeq2 EBSeq Limma NOISeq Cufflinks 204 92 90 19 1 DESeq2 1572 1269 281 10 EBSeq 4600 324 11 Limma 342 10 NOISeq 11 DS CufflinksDS DEXSeq LimmaDS SplicingCompass CufflinksDS 0 0 0 0 DEXSeq 1727 82 35 LimmaDS 103 7 SplicingCompass 91 Conclusions In this study, we performed a systematic evaluation of workflows for DIE and DS analysis using simulated RNA-seq data sets based on a real human experiment. The goal of our work was to provide guidelines for choosing appropriate analysis strategies for researchers interested in different modalities of isoform expression changes. For this, we evaluated nine workflows in a variety of expression setups and experimental configurations. Simulated data sets are one way to perform this evaluation enabling workflow comparison by means of controlled experiments. Our simulation settings were designed with the goal to mimic, as closely as possible, the real case. For this, the RSEM simulation tool was selected because of its ability to estimate and incorporate parameters learned from real data. However, it is well known that biology is more complicated than simulated data. In particular, biological data is typically more complex than simulations: genes do not fall into artificially defined classes and confounding factors such as outliers and hidden experimental or technical biases may be present . In our case, the lack of real data sets with standardized and well annotated AS events  lead us to use the presented simulated strategy acknowledging their limitations. Three experimental scenarios with balanced designs were simulated here, considering less (four) and more (eight) replicates per condition. Although balanced designs are particular cases, unbalanced experiments may lead to less precise estimations of some of the experimental factors as well as negative impact on the statistical power for both generalized linear or linear models [30, 31]; thus, experimental design should be carefully considered. We also tailored our analysis to human transcriptomics data sets with variability similar to tumour-healthy subject samples. The validity of our study for other types of data (i.e. cell-lines or organisms with lower complexity) and for other experimental configurations (i.e. with different numbers of replicates or unbalanced designs) remains to be demonstrated. In general terms, we found that a better scenario for case–control comparisons was when more differential genes (10% versus 5%) and replicates per condition (8 versus 4) were available (S3). For this configuration, we found the highest number of DI/ASG, TPs and concordance among replicated simulation. Best performing workflows were DESeq2, Limma and NOISeq for DIE analysis and DEXSeq and LimmaDS for DS testing. We used precision, sensitivity and F-score as performance measures. For experiments with a low number of replicates, the best pipelines to DIE analysis were DESeq2 and Limma. Also, high stability of those performance measures was observed when several isoform expression levels and lengths were analysed. Based on our results, we concluded that, if high sensitivity is preferred, DESeq2 is the most indicated, while the Limma pipeline should be used if higher precision is important. For experiments with a large number of replicates, NOISeq is more restrictive and precise than Limma. For DS pipelines, we found that DEXSeq was the best in terms of sensitivity and F-score. However, precision of this method was lower than the one achieved by LimmaDS, which reached the DEXSeq F-score values when the number of replicates was increased. The performance of DS workflows resulted to be more influenced by expression levels than gene length. In particular, better results were observed for genes with >50 counts and length >470 nts. We concluded that these two workflows are indicated for DS analysis, the first one prioritizing sensitivity and the second precision. When the FPR was evaluated, we found that both Limma and LimmaDS workflows were superior to DESeq2 and DEXSeq, respectively. We also evaluated the effect of the number of isoforms per gene in the percentage of TPs and FPs. DIE pipelines were found to be more influenced by the number of isoforms per gene than the DS workflows, probably by the presence of low-expressed isoforms. In addition, the TPRs for DIE workflows decreased as the number of isoforms per gene increased. In particular, we found TPR between 30% and 90% using four replicates for DESeq2 and Limma, whereas, NOISeq reached values between 25% and 60% when more replicates were available. Using DEXSeq or LimmaDS, we found near to 40% of TPs with fewer replicates. Meanwhile, TPR increased to 60% when more replicates were available. We also found that, in DIE and DS cases, most of the FPs were related to genes with more than nine isoforms. Exploring the effect of the magnitude of DE on TPRs, we noted that this was higher for isoforms with greater expression changes, with or without changes in the AS. In the case of DESeq2 and NOISeq, TPs detection was further improved when the percentage of differentially expressed genes was higher. This suggests that these pipelines benefit from an extended regulation in the data and might have problems in detecting DE when this affects only a small subset of transcripts. Controversially, Limma associated better performance with more replicates. In the case of DS workflows, we found that DEXSeq achieved the highest TPRs percentages, followed by LimmaDS. Both of those genes found under DS and DS combined with changes in isoform expression levels, with better results in the latter. Those pipelines also showed higher percentages when more replicates were used. In both, DS and DIEDS groups, the best results were found for genes with the largest change in the major isoform expression. Finally, we suggest that if the number of replicates per condition is low, the workflows based on the Limma R package could be used to detect DIE and DS with high precision. The use of DESeq2 and DEXSeq workflows might be preferred when a high number of genes/isoforms are expected. If the number of replicates per condition is higher, we recommend the use of NOISeq workflow for DIE analysis combined with any of LimmaDS or DEXSeq pipelines for DS analysis. We designed an schema of workflow selection, showed in Figure 7, summarizing our results. For DS pipelines, the newest aligner STAR  can be used instead of TopHat2 with similar performances (see Supplementary Figure S10). Key Points A number of workflows have been developed to either analyse differential gene or transcript expression and differential splicing using RNA-seq data. However, there is no clear consensus about the best practices for the simultaneous exploration of both types of transcriptional regulation. Our work analysed nine different pipelines or workflows to provide guidelines. The choice of workflows choice directly impacts on the number of detected differential features (isoforms or genes) and the sensitivity and precision of the result. The number of isoforms per gene and the magnitude of the expression change influence the power of true detections. Fewer isoforms per gene and larger expression changes favour the detection of true positive differential features. The number of replicates and the amount of expected differentially expressed genes/isoforms between conditions should be taken into account when selecting the analysis pipeline. Workflows based on DESeq2 and DEXSeq are recommended for experiments with few heterogeneous samples; whereas, Limma and NOISeq pipelines will return better overall results when more replicates are available. Funding This work was supported by the Developing an European-American NGS Network (DEANN) project-Marie Sklodowska-Curie Actions financed by the European Commission, the Spanish MINECO [grant no. BIO2012-40244 to A.C.] and the following Argentine institutions: Universidad Católica de Córdoba [grant no. BOD/2016 to E.A.F.], Ministerio de Ciencia, Tecnología e Innovación Productiva [grant no. PPL6/2011 to E.A.F.], Secretaría de Ciencia y Tecnología de la Universidad Nacional de Córdoba [grant no. 30720150101719CB to E.A.F.], Universidad Nacional de Villa María and the Consejo Nacional de Investigaciones Científicas y Técnicas. Gabriela A. Merino is a PhD student in Engineering Sciences and a Professor of Mathematical Analysis II at the National University of Córdoba, Argentina. Her doctoral research focuses on bioinformatics and statistical analysis of the next-generation sequencing data and is funded by the National Council of Science and Technology of Argentina (CONICET). Ana Conesa is a bioinformatician and computational biologist developer of many different bioinformatics tools for functional annotation and analysis of transcriptomics data, quality assessment of short-/long-read sequencing data and multiomics integration. She enjoys a shared position as Head of the Genomics of Gene Expression Lab at the Centro de Investigación Príncipe Felipe (Valencia, Spain), and as Professor Bioinformatics at the Microbiology and Cell Sciences Department of the University of Florida at Gainesville, FL, USA. Elmer A. Fernández is an Independent Researcher at National Council of Science and Technology of Argentina (CONICET). He is a bioengineer from the National University of Entre Rios and he got his PhD in Advanced Computing and Artificial Intelligence from the University of Santiago de Compostela, Spain. He currently leads the Bioscience Data Mining Group at CIDIE-CONICET-UCC in Córdoba, Argentina. He also leads de BioDataMining node of the National Bioinformatic Platform. References 1 Wang J , Ye Z , Huang THM , A survey of computational methods in transcriptome-wide alternative splicing analysis . Biomol Concepts 2015 ; 6 ( 1 ): 59 – 66 . 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Briefings in Bioinformatics – Oxford University Press
Published: Mar 25, 2019
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