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- Publisher
- Nature Publishing Group
- Copyright
- Copyright © 2014 Nature Publishing Group, a division of Macmillan Publishers Limited. All Rights Reserved.
- ISSN
- 1087-0156
- eISSN
- 1546-1696
- D.O.I.
- 10.1038/nbt.2921
- Publisher site
- See Article on Publisher Site
Abstract
A complete accounting of gene expression in an organism would provide the exact sequence and location of every transcript in every cell. This goal is a long way off, but significant progress has been made through decades of technology development in sequencing and imaging. On one hand, RNA sequencing (RNA-seq) has made it possible to measure gene expression on a genome-wide scale, typically in cell populations . On the other hand, RNA fluorescence in situ hybridization (FISH) has advanced to the point where we can detect individual RNA molecules in single cells through a microscope, gaining accurate, absolute single-cell expression data together with information on RNA location down to the subcellular level, but usually for just a few genes at a time . Writing in Science , Lee et al . report an exciting first step toward combining some of the best aspects of both methods to sequence RNA in single cells in situ . Their approach, called fluorescence in situ RNA sequencing (FISSEQ), effectively treats the cell as a sequencing chip while preserving spatial information about RNA location. The authors begin by fixing cells, reverse transcribing the RNA in situ and copying the resulting cDNAs by rolling-circle
Journal
Nature Biotechnology – Nature Publishing Group (NPG)
Published: Jun 9, 2014
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