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, blocks late origin firing. Upon exposure to DNA damaging agents, cells expressing nonphosphorylatable alleles of SLD3 and DBF4 ( SLD3 -m25 and dbf4-m25, respectively) proceed through S-phase faster than wild ...
cell cycle, and found overlap with respect to specific phosphorylation events, including those that are important for blocking late DNA replication origin firing in an S phase checkpoint response ...
phosphorylates two essential initiation factors Sld2 and Sld3 , which results in their phospho-dependent interaction with the BRCT repeats of Dpb11 [8,9]. This CDK-dependent complex results in the recruitment ...
in late S and G2 phase, and phosphorylation at sites of 19, 32 and 110 was maximally detected in M phase (Fig. 7). Thus, the phosphorylation of MCM4 at these sites may play an important role in dislodging ...
hyper-acetylation is the phosphorylation of Dbf4 and Sld3 , which prevents the firing of late origins. Our results demonstrate that under these circumstances Dun1 is essential, implicating Dun1 ...
a variety of cellular processes including cell cycle progression, DNA damage repair, chromatin remodeling, and transcription. The DDR induces a number of physiological changes within the cell, including ...
( Sld3 -Sld7) complex to pre-RCs, which in turn interacts with and recruits Cdc45 [4–6]. S-CDK facilitates origin firing by phosphorylating Sld3 and Sld2 [7–9]. Phospho- Sld3 interacts with DNA polymerase ...
(ARS607) was not affected (S1A Fig, left and middle panels). Origins that fire early in S phase, but not late -replicating regions, recruit the pre-replicative complex (pre-RC) component Sld3 in G1, prior ...
is recruited to chromatin through interaction with phosphorylated serine 128 of histone H2A (γ-H2AX, a DNA damage - induced modification) and methylated histone H3 lysine 79 (H3K79me) [24–27], the latter being ...
-dependent kinase; DDK) phosphorylates MCM subunits. Sld3 in cooperation with Sld7 and Cdc45 recognizes Mcm4 as well as other Mcm subunits that are phosphorylated by Cdc7 (14–16). Second kinase, cyclin ...
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