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Assessment of Trace Metals in Sediments from Khnifiss Lagoon (Tarfaya, Morocco)

Assessment of Trace Metals in Sediments from Khnifiss Lagoon (Tarfaya, Morocco) Article Assessment of Trace Metals in Sediments from Khnifiss Lagoon (Tarfaya, Morocco) 1 , 2 2 2 2 Ali Tnoumi * , Massimo Angelone , Giovanna Armiento , Raffaela Caprioli , Cinzia Crovato , 2 2 2 , † 3 2 Maurizio De Cassan , Maria Rita Montereali , Elisa Nardi , Luisa Parrella , Marco Proposito , 2 1 Fabio Spaziani and Bendahhou Zourarah LGMSSURAC45, Faculty of Sciences, Chouaib Doukkali University, El Jadida 24000, Morocco; zourarah.b@ucd.ac.ma ENEA C.R. Casaccia, Department for Sustainability, 00123 Rome, Italy; massimo.angelone@enea.it (M.A.); giovanna.armiento@enea.it (G.A.); raffaela.caprioli@enea.it (R.C.); cinzia.crovato@enea.it (C.C.); maurizio.decassan@enea.it (M.D.C.); mariarita.montereali@enea.it (M.R.M.); elisa.nardi@isprambiente.it (E.N.); marco.proposito@enea.it (M.P.); fabio.spaziani@enea.it (F.S.) ENEA C.R. Portici, Department for Sustainability, Portici, 00196 Naples, Italy; luisa.parrella@enea.it * Correspondence: tnoumi.ali@gmail.com or tnoumi.a@ucd.ac.ma; Tel.: +212-664-649-183 † Now working at ISPRA—The Italian National Institute for Environmental Protection and Research, 00144 Rome, Italy. Abstract: Surface sediments from Khnifiss lagoon (Morocco) were analyzed to evaluate the con- tamination degree of the area. Concentrations of V, Cr, Co, Ni, Cu, Zn, As, Cd, Hg, and Pb were determined on samples taken during the summer and the autumn of 2016. On the whole, higher concentrations were found in the summer season. The results revealed the following average con- centrations (mg/kg), reported in descending order: Zn (51.7  31.3) > V (38.8  24.7) > Cr (26.6 17.8) > Ni (16.5  5.47) > As (8.50  2.00) > Cu (6.60  3.81) > Pb (6.13  3.46) > Co (3.57  2.09) > Cd (0.16  0.11) > Hg (0.006  0.001). Organic matter showed a positive significant correlation with some trace metals (mainly V, Cr, Co, Zn, Cd, Pb). Three pollution indices were calculated: Citation: Tnoumi, A.; Angelone, M.; Enrichment Factor (EF), Index of Geoaccumulation (Igeo), and Pollution Load Index (PLI). Minimal Armiento, G.; Caprioli, R.; Crovato, enrichments (for Zn, As, and Cd) were detected at some sampling points. Overall indices showed C.; De Cassan, M.; Montereali, M.R.; that the Khnifiss sediments can be classified as not contaminated, and that the trace metals amounts Nardi, E.; Parrella, L.; Proposito, M.; found are ascribable to the geogenic origin. The results of this work can be used as a starting point et al. Assessment of Trace Metals in for further evaluations of trace metals distribution in Moroccan lagoons. Sediments from Khnifiss Lagoon (Tar- faya, Morocco). Earth 2021, 2, 16–31. Keywords: Khnifiss Lagoon; trace metals; enrichment factor; sediment geochemistry; contamina- https://dx.doi.org/10.3390/earth201 tion assessment Received: 19 November 2020 Accepted: 7 December 2020 1. Introduction Published: 23 December 2020 Coastal lagoons are very important environments, both in ecological and in economic Publisher’s Note: MDPI stays neu- terms, since they provide the habitat for numerous species and are the backbone of various tral with regard to jurisdictional claims anthropic activities (including food-related ones and tourism) [1,2]. However, the human in published maps and institutional exploitation of these ecosystems implies a significant potential environmental vulnerability, affiliations. especially due to their geo-morphological setting (as semi-enclosed area) and their link to terrestrial inputs and continental run-off [3,4]. Indeed, the presence of trace metals in lagoons and coastal zones has been attributed mutually to natural events (volcanism, flash flood, wind transport, erosion) and anthropogenic processes (vehicle emission, in- Copyright: © 2020 by the authors. Li- dustry, urbanization, construction activities, mining activities) [5–8]; for these reasons, censee MDPI, Basel, Switzerland. This lagoon sediments can act both as geogenic source and as a final sink for trace metals in article is an open access article distributed the aquatic ecosystems [9,10]. They can be considered as good geo-indicators of marine under the terms and conditions of the environmental quality, since their status can alter benthos and the food chain, thus posing Creative Commons Attribution (CC BY) potential ecological risks [11,12]. Therefore, the study of coastal sediments provides useful license (https://creativecommons.org/ licenses/by/4.0/). information on marine pollution [13]. Trace metals are persistent in the environment and Earth 2021, 2, 16–31. https://dx.doi.org/10.3390/earth2010002 https://www.mdpi.com/journal/earth Earth 2021, 2 17 are subject to bioaccumulation (which often causes irreversible adverse effects on ani- mals and humans) [14,15]. In addition, they represent a global-scale environmental issue, since serious problems caused by this category of pollutants are known to exist in many areas of the world (both in developed and developing countries) [16,17]. More than 90% of trace metals load in aquatic systems is associated with suspended particulate matter and sediments [18,19]. The fate and transport of these elements in marine area and lagoons are controlled by a variety of factors such as redox potential, texture, ionic strength, the abun- dance of adsorbing surfaces, pH, and organic matter content [20,21]. The environmental monitoring helps to develop effective management plans, to improve the strategies for coastal activities, and to distinguish between the anthropogenic and the natural sources of the trace metal enrichments. Morocco acts as an especially relevant site for the study of coastal ecosystems, since it holds five lagoons: Four facing the Atlantic Ocean (Khnifiss, Sidi Moussa, Oualidia, and Moulay Bousselham lagoons) and one facing the Mediterranean Sea (Marchica La- goon), covering about 3500 Km of the national coastline. The present study focuses on the most important desert wetland in the Saharan coast of Morocco and of North Africa (RAMSAR site since 1980): The lagoon of Khnifiss. It is a shallow tidal lagoon of open water in an arid environment, where the supply of continental material is strongly lim- ited to aeolian particle inputs; in addition, a coastal upwelling from the Canary Islands induces high biogenic accumulation rates [22]. Although Khnifiss Lagoon is currently protected, information on its sediment’s metal concentrations is still lacking. The relevance of Khnifiss Lagoon is further strengthened considering that there is a proposal to use the area as a reference site for the evaluation of trace metal contamination levels, since it is characterized by abundant and various macrobenthic fauna [23], low density of human population (1.07 per/km at Tarfaya Province) [24], and it is far from major cities and industrial activities. The main objectives of this research are to determine the trace metal contents in the Khnifiss Lagoon, in order to assess the contamination degree of the area; to carry out a preliminary screening that can be used as starting-point for a more detailed activity of geochemical study. 2. Materials and Methods 2.1. GeoEnvironmental Setting of the Area The study area is located in the Province of Tarfaya, between Cap Nachtigal and Cap Juby, in a rural region on the Southwest side of Morocco (27.80–28.05 N, 12.21–12.39 W). It extends for about 20 km, with a maximum of about 5 km width, at Sabkha Tazera [25]. The site is considered one of the most important zones for birds’ migrations. Besides, it provides the livelihood of people living in adjacent areas, where most activities are related to fishing and nature tourism. The lagoon is connected to the Atlantic Ocean through Foum Agoutir at its Northern side. The tidal regime is semidiurnal, and it ranges between a minimum of 1.48 m down- stream to a maximum of 2.54 m inside the lagoon. The speed of the tidal current exceeds 91 cm/s at Foum Agoutir. The residence time of the water in the lagoon is 4.4 tidal cycles during neap tides and 1.5 tidal cycles during spring tides [26]. Salinity is between 30‰ and 40‰ in the downstream zone, between 32‰ and 45‰ in the intermediate zone, and be- tween 37‰ and over 45‰ in the upstream zone due to the saltworks at Sebkha Tazera [27]. The phytoplankton community is dominated by diatoms (90%) and dinoflagellates (10%), with Chaetoceros (88.5%) and Amphora (6.7%) being the main exponents of the popula- tion [28]. The lagoon has varieties of floral species such as Zygophyllum geatulum, Launaea arborescens, Lycium intricatum, Cistanche phelypaea, Zostera noltii, and Spartina maritima [29]; seaweeds are mainly represented by 24 species (4 Chlorophyceae, 10 Phaeophyceae, and 10 Rhodophyceae) [30]. The air temperature ranges between a minimum of 15.8  2.5 C to a maximum of 24.0  2.2 C with an average of 19.9  2.2 C. The precipitations are classified as extremely rare, with an average of 0.3  0.2 mm daily and 92.6  58.5 mm annually Earth 2021, 2 18 over the last three decades (1994–2019). The wind speed ranges between 4.47  0.53 m/s to 7.90  0.82 m/s, with an average of 6.13  0.41 m/s. Earth 2020, 1, FOR PEER REVIEW 3 The geomorphology of the region is conditioned by the presence of Cretaceous marl or marly-limestone, to which it is over-imposed a harder calcareous-sandstone (Marine The geomorphology of the region is conditioned by the presence of Cretaceous marl or marly- Moghrebien) protective slab. From a geological point of view (Figure 1), the study area limestone, to which it is over-imposed a harder calcareous-sandstone (Marine Moghrebien) is located in the North-Eastern side of Tarfaya coastal basin, formed during the Mesozoic protective slab. From a geological point of view (Figure 1), the study area is located in the North- and the Cenozoic seaward of the stable West African craton [31]. The main sources of the Eastern side of Tarfaya coastal basin, formed during the Mesozoic and the Cenozoic seaward of the sediments are the AntiAtlas in the Est, and the Reguibat dorsal and Mauritanides in the stable West African craton [31]. The main sources of the sediments are the Anti−Atlas in the Est, and Southern part [32,33]. the Reguibat dorsal and Mauritanides in the Southern part [32,33]. Figure 1. Geological map of the area surrounding the Khnifiss Lagoon (Digitalized from [34]). Figure 1. Geological map of the area surrounding the Khnifiss Lagoon (Digitalized from [34]). 2.2. Sediment Sampling and Analysis 2.2. Sediment Sampling and Analysis SurSurface face sedisediments ments werewer collect e collec ed in ted 201 in 6, 2016, during during two se two asonseasons s (summ(summer er and autand umnautumn), ), at six different stations (S1–S6), for a total of 12 samples, ensuring spatial coverage of the lagoon (Figure 2). at six different stations (S1–S6), for a total of 12 samples, ensuring spatial coverage of The sediments were taken using a stainless steel Van Veen grab sampler, between 0–5 cm depth. The the lagoon (Figure 2). The sediments were taken using a stainless steel Van Veen grab tools were washed with lagoon water before sampling. The sediments were stored in airtight sampler, between 0–5 cm depth. The tools were washed with lagoon water before sampling. polyethylene bags, labeled, and immediately frozen at −20 °C until further processing. The location The sediments were stored in airtight polyethylene bags, labeled, and immediately frozen of each samp  le was recorded by a GPS. At the laboratory, the sediments were defrosted by keeping at 20 C until further processing. The location of each sample was recorded by a GPS. Earth 2021, 2 19 At the laboratory, the sediments were defrosted by keeping them at room temperature for about 24 h, then dried in an oven at 40 C (it has been proved by Gilli et al. [35] that samples drying at 40 C does not cause evaporation losses of Hg). The samples were at last crushed using a mortar grinder, homogenized, stored inside polyethylene bags until the chemical analyses were carried out. Earth 2020, 1, FOR PEER REVIEW 5 Figure 2. Map of the sampling locations at Khnifiss Lagoon. Figure 2. Map of the sampling locations at Khnifiss Lagoon. Earth 2021, 2 20 The determinations of trace metals (except Hg) were accomplished using a microwave- assisted acid dissolution procedure, according to EPA method 3052, followed by the chemical analyses performed by: (i) An ICP-MS instrument (Perkin Elmer ELAN 6100) for the quantification of V, Cr, Co, Ni, Cu, Zn, As, Cd, and Pb (according to EPA method 6020 b); (ii) an ICPOES instrument (Perkin Elmer Optima2000 DV) for Al (according to EPA method 6010 d). All the reagents were of analytical grade. The analysis of Hg was carried out by the automated mercury analyzer FKV AMA-254; the advantage of this device is that Hg is directly determined on the dry sample and the acid dissolution step is not required [36]. Precision and accuracy were verified using marine sediment reference certified materials (PACS-3 and MESS-4; National Research Council Canada, NRCC): The recoveries of Al, V, Cr, Co, Ni, Cu, Zn, As, Cd, Hg, and Pb in MESS4 ranged from 84.0 to 118.5%; the recoveries of Al, V, Cr, Co, Ni, Cu, Zn, As, Cd, and Pb in PACS3 ranged from 88.1 to 100.5%. For all the elements, the quantitation limits were: V, Co, As, Pb = 0.05 mg/kg; Cd = 0.03 mg/kg; Cr = 0.5 mg/kg; Ni, Cu = 0.3 mg/kg; Zn = 1.0 mg/kg; Hg = 0.005 mg/kg; Al = 50 mg/kg. Organic matter (OM), which in sea sediments originates from marine and terrestrial sources, was determined by LossOnIgnition procedure (LOI) [37]. About 4 g of each sediment were weighed and transferred in a porcelain crucible. The samples were heated at 105 C for 24 h to remove the moisture content and the new weight was determined; then, the samples were kept in a muffle furnace at 550 C for 4 h and weighed again. Lastly, the organic matter was calculated according to Equation (1): Weight Weight 105 C 550 C LOI =   100 (1) Weight 105 C The calcium carbonate content (CaCO ) was measured by volumetric calcimetry, using a Bernard calcimeter [38,39]. Grain size measurement was determined using both dry and wet separation techniques [40–42]: The samples were pre-treated with H O and 2 2 washed with bidistilled water to remove organic matter and chlorides, then oven-dried at 40 C for 24 h; the coarse fraction was isolated by sieving, while the fine fraction (<63 m) was ultrasonically dispersed using 0.5~10% sodium hexametaphosphate (NaPO ) for 24 h 3 6 and analyzed by a particle size analyzer (Micromeritics, SediGraph). 2.3. Pollution Indices To assess the levels of trace metal contamination and the possible anthropogenic impact on the sediments, three of the most used pollution indices were applied [43,44]: Enrichment Factor (EF), Index of geo-accumulation (Igeo), and Pollution Load Index (PLI). The calculations of the indices require the use of reference values from a background matrix, and the Shale data proposed by Turekian and Wedepohl [45] were chosen for this study (because local geochemical background values were not available). The EF is used to evaluate the degree of anthropogenic influence on the occurrence of a metal in the soil/sediment; it is calculated through the ratio of the metal concentration in the sample with respect to the background matrix, using a normalizing element [46]. Several conservative elements have been used for normalization purposes [47–49], but we selected Al since it is generally the major constituent of clay minerals. The EF for a trace metal is expressed as follow (Equation (2)): (C /C )Sample n Al EF = (2) (C /C )Background Al where C and C are the concentrations of the trace metal (n) and Al. n Al Earth 2021, 2 21 The Igeo index, originally developed by Muller [50], was used to quantify the metal contamination related both to natural events and/or human activities [51]. It is calculated according to Equation (3): Igeo = log (3) k  B where C is the concentration of the metal (n) in the sample and B is the geochemical n n background value of the same element [52]. The constant k = 1.5 is used as a correction coef- ficient for natural fluctuations in background values as well as for very small anthropogenic influences [50]. PLI provides an integrated and site wise comparative assessment of cumulative trace metal pollution [53]. This index can be interpreted as an appraisal of the comprehensive trace metal pollution in a given sample. It is calculated using the Equations (4) and (5): PLI = (CF )  (CF )  (CF )  . . .  (CF ) (4) 2 3 n CF = C /C (5) Sample Background where CF is the contamination factor, C is the concentration of a selected metal in Sample the sample, and C is the amount of the same element in the background matrix. Background Each index evaluates the sample quality according to the scores and classes [53–55] reported in Table 1. Table 1. Scores and classes of Pollution Load index (PLI), Index of geoaccumulation (Igeo), and Enrichment Factor (EF). PLI [53] Degree of Contamination Igeo [54] Degree of Contamination EF [55] Degree of Contamination <1 Unpolluted Igeo  0 Uncontaminated EF < 2 No enrichment/Depletion to mineral >1 Polluted 0 < Igeo  1 Uncontaminated to moderately 2  EF< 5 Moderate enrichment 1 < Igeo  2 Moderately contaminated 5  EF < 20 Significant enrichment 2 < Igeo  3 Moderately to highly contaminated 20  EF < 40 Very high enrichment 3 < Igeo  4 Highly contaminated EF  40 Extremely enrichment 4 < Igeo  5 Highly to extremely contaminated Igeo > 5 Extremely contaminated 2.4. Correlation Analysis The data processing was performed using R statistical software [56]. Mean, maximum, minimum, standard deviation, first quartile, third quartile, and Skewness were calculated. The Shapiro-Wilk test was used to assess the normality of the dataset. Since the data were not normally distributed, the Spearman correlation coefficient was used in order to identify any significant pattern and relationship between the variables. 3. Results and Discussion 3.1. Geochemical Results The complete dataset and the descriptive statistics are summarized in Table 2. The sed- iments of the lagoon showed a moderately alkaline pH, which ranged from 7.55 to 8.27, and exhibited spatial differences in the occurrence of trace metals (Figure 3). Stations S–2, S–4, and S–6 revealed higher concentrations compared to other locations, probably due to different grain size distribution; these samples, indeed, may have a larger accumulation of fine grained terrigenous and marine materials as reflected by their higher amounts of Al. In the summer season, station S–4 stands out for the higher concentrations exhibited for all the elements. In autumn, instead, S–6 has higher concentrations of V, Cr, Co, Cu, Zn, As, Hg, and Pb, while S–2 and S–4 had the maximum amounts of Ni and Cd. The OM in sediments is a key parameter since it is related to the ability to concentrate (either by ion exchange and/or complexation) and release metals in marine and lagoon waters, and it is also a source of nutrients for aquatic fauna [57]. It ranged between 1.50 and 7.56%, with an average of 3.78% (Figure 4). The lowest amounts were observed, in both seasons, at S–1 (Foum Agoutir, inlet of the lagoon), due to high hydrodynamic characteristic Earth 2021, 2 22 at this station and for the prevalence of sandy texture [26]. Generally, sediments in rough waters are sandy and then exhibit lower metal contents [47]. On the contrary, the maximum amounts of OM, that speed up the adsorption of the metals from the overlying water to bottom sediments, were recorded at station S–2 and S–4 due to the presence of seagrass, which encourages the retention of the fine particles. The relatively higher concentrations observed during the summer season for most of the trace metals may be attributed to the small differences in OM contents, having an average of 3.92  2.63% compared to the 3.65  2.28% in autumn. The lower OM in fall may be a consequence of elevated decomposition rates fostered by high levels of dissolved oxygen [27]. However, the lower metal concentrations found during the autumn might be also related to their mobilization from the sediments promoted by the overlying water. The ranges of OM percentages were compared to other Moroccan Lagoons, e.g., Moulay Bousselham Lagoon (1.24–15.74%) [58] and Oualidia lagoon (7.56–15.23%) [59]. The comparison showed that the OM values in the Khnifiss Lagoon sediments are about two times lower than the ones from the other local lagoons. This could justify the low concentration values of trace metals measured in this lagoon. Lefrere et al. [23] achieved the same conclusion using benthic fauna. In addition, the lagoon receives neither freshwater supply from the mainland nor waste discharges, and therefore is characterized only by a continuous renewal of water according to the current tidal trend [26]. The carbonate concentrations varied in a range of 8.25–58.8% and the highest values were registered, in both seasons, in stations S1 and S3. These results are reasonably related to the nature of the sediments since the presence of limestone fragments (from shells and snails) causes the rise of carbonate contents. The importance of determining and studying carbonates in marine systems is related to their ability to adsorb metal forming labile species [60]. Overall, the means of the analyzed elements are below the average shale’s values [45,61,62] (Table 2) indicating low metal concentrations in the lagoon sediments (Figure 2). The world average shale value represents the background concentration of metals in a collection of several sedimentary rocks derived from argillaceous materi- als worldwide. 3.2. Contamination Degree Assessment Pollution indices may help to quantify the level of enrichment of a sediment with trace metals [63]. The EFs calculated on the dataset are reported in Table 3. All the stations showed no enrichments (EF < 2) for V, Cr, Co, Ni, Cu, Hg, and Pb, both concerning the summer and the autumn, thus indicating concentration levels close to the natural background for marine sediments [55]. Moderate enrichments (2  EF < 5) were found: For Cd (sample S4) and Zn (sample S6) in summer, that can be linked to the upwelling phenomenon of cold water masses on the southern Atlantic coasts of Morocco. Our findings corroborate with results of Idardare et al. [64] using Nereis diversicolor as bioindicator; for As in both the seasons (S1, S3, S5 in summer and S1, S3, S5, S6 in autumn), that can be attributed to the lithology of this area and especially to the presence of schist rocks [64,65]. On the whole, EF index values showed the following trend in summer As > Zn > Cd > Pb > V > Cr > Ni > Co > Cu > Hg and the following one in autumn As > Cd > Zn > Pb > V > Cr > Ni > Co > Cu > Hg. Nevertheless, according to Atgin et al. [66], EF values less than 5 cannot be considered noteworthy, and a minor enrichment can be related to the effect of the lithology. The Igeo results are summarized in Figure 5, showing that all the sampling stations can be classified as “uncontaminated” for the studied trace metals (Igeo < 0). The PLI values for all the samples ranged from 0.07 to 0.43, with averages of 0.22 and 0.20 in summer and autumn, respectively. The PLI trends were as follow: S4 > S2 > S6 > S5 > S3 > S1 in summer, and S6 > S2 > S4 > S5 > S3 > S1 in autumn (Figure 6). This index supports again the evidence that no appreciable input from anthropogenic sources affects the area (PLI < 1). Earth 2021, 2 23 Table 2. Geochemical analysis results and descriptive statistics in surface sediments from Khnifiss Lagoon. Al V Cr Co Ni Cu Zn As Cd Hg Pb OM CaCO Clay Silt Sand pH % mg/kg mg/kg mg/kg mg/kg mg/kg mg/kg mg/kg mg/kg mg/kg mg/kg % % % % % S1 1.79 11. 5 7.50 1.36 12.3 2.23 15.9 6.70 0.06 <0.005 2.89 8.20 1.80 54.5 0.03 0.80 99.2 S2 4.05 60.7 44.3 5.90 22.9 10.1 81.1 8.92 0.29 0.01 10.8 7.68 7.36 29.6 20.4 45.5 34.1 S3 1.46 17.4 12.2 1.71 13.8 2.79 22.5 6.48 0.10 <0.005 3.44 7.89 2.25 51.9 1.80 2.80 95.4 S4 4.78 76.8 57.8 7.13 26.1 12.6 101 11.1 0.40 0.01 12.4 7.59 7.16 13.3 22.4 58.6 19.1 S5 2.10 24.6 15.4 2.40 15.0 5.77 36.6 8.57 0.09 <0.005 3.63 8.27 1.88 33.8 0.26 1.10 98.6 S6 2.72 61.9 35.5 4.47 13.8 8.38 81.4 8.64 0.14 <0.005 5.71 7.82 3.07 22.4 5.54 7.82 86.6 S1 1.45 10.9 7.74 1.21 9.95 1.95 15.0 6.70 0.06 <0.005 2.52 8.24 1.50 58.8 0.24 0.62 99.1 S2 3.47 48.0 32.8 4.80 19.0 9.50 65.9 8.33 0.24 0.01 8.85 7.55 7.56 28.4 16.3 37.4 46.3 S3 1.56 16.7 10.4 1.59 10.1 2.77 22.5 6.49 0.09 <0.005 3.24 7.76 2.34 45.1 0.51 2.50 97.0 S4 3.41 47.1 34.5 4.36 23.0 9.71 65.4 8.87 0.23 0.01 7.17 7.93 3.93 23.8 10.3 21.4 68.3 S5 1.78 19.2 12.2 1.99 12.3 3.60 28.0 8.04 0.10 <0.005 3.64 7.88 1.88 32.6 0.10 0.80 99.1 S6 4.04 70.8 49.1 5.88 19.8 9.77 85.0 13.2 0.18 0.01 9.29 7.72 4.68 8.25 29.4 29.3 41.3 Minimum 1.45 10.9 7.50 1.21 9.95 1.95 15.0 6.48 0.06 0.01 2.52 7.55 1.50 8.25 0.03 0.62 19.1 Maximum 4.78 76.8 57.8 7.13 26.1 12.6 101 13.2 0.40 0.01 12.4 8.27 7.56 58.8 29.4 58.6 99.2 Mean 2.72 38.8 26.6 3.57 16.5 6.60 51.7 8.50 0.16 0.01 6.13 7.88 3.78 33.6 8.94 17.4 73.7 St.dev. 1.18 24.7 17.8 2.09 5.47 3.81 31.3 2.00 0.11 0.00 3.46 0.25 2.35 16.1 10.6 20.6 30.4 1 Quartile 1.72 17.3 11.7 1.68 12.3 2.78 22.5 6.70 0.09 0.01 3.39 7.71 1.88 23.5 0.26 1.03 45.1 3 Quartile 3.61 61.0 37.7 5.07 20.6 9.73 81.2 8.88 0.24 0.01 8.96 8.00 5.30 46.8 17.3 31.3 98.8 Ref. data: Average 8 130 90.0 19.0 68.0 45.0 95.0 13.0 0.30 0.40 20.0 - - - - - - Shale Bold: autumn season; italic: summer season. Earth 2021, 2 24 Earth 2020, 1, FOR PEER REVIEW 9 (a) (b) (c) (d) (e) (f) (g) (h) Figure 3. Cont. Earth 2020, 1, FOR PEER REVIEW 11 Earth 2021, 2 25 Earth 2020, 1, FOR PEER REVIEW 10 (g) (h) (i) (j) (i) (j) Figure 3. Spatial variations of the investigated metals during summer and autumn of the surface Figure 3. Spatial Figure 3. variations Spatial v of the ariat investigat ions of the inv ed metals estig during ated m summer etals during summer an and autumn of the d autumn of the surface surface sediments from Khnifiss sediments from Khnifiss Lagoon: (a) Vanadium; (b) Chromium; (c) Cobalt; (d) Nickel; (e) Copper; (f) Lagoon: (a)sediments from Khnifiss Vanadium; (b) Chromium; Lagoon: (c) Cobalt; (a) Va(na d)d Nickel; ium; (b() Chro e) Copper; mium; (f ( ) c Zinc; ) Cob(a gl) t; ( Arsenic; d) Nick( eh l;) (Cadmium; e) Copper; ( (i f) ) Mercury; Zinc; (g) Arsenic; (h) Cadmium; (i) Mercury; (j) Lead. Horizontal solid red line represents the Average (j) Lead. Horizontal solid red line represents the Average Shale Background value. Zinc; (g) Arsenic; (h) Cadmium; (i) Mercury; (j) Lead. Horizontal solid red line represents the Average Shale Background value. Shale Background value. Figure 4. Organic matter (%) distribution in Khnifiss Lagoon during summer and autumn. Figure 4. Organic matter (%) distribution in Khnifiss Lagoon during summer and autumn. 3.2. Contamination Degree Assessment Table 3. Enrichment Factor (EF) in the surface sediments from Khnifiss Lagoon. Pollution indices may help to quantify the level of enrichment of a sediment with trace metals [63]. The EFs calculated on the dataset are reported in Table 3. All the stations showed no enrichments V Cr Co Ni Cu Zn As Cd Hg Pb Figure 4. Organic matter (%) distribution in Khnifiss Lagoon during summer and autumn. (EF < 2) for V, Cr, Co, Ni, Cu, Hg, and Pb, both concerning the summer and the autumn, thus S1 0.39 0.37 0.32 0.81 0.22 0.75 2.30 0.86 0 0.65 indicating concentration levels close to the natural background for marine sediments [55]. Moderate S2 0.92 0.97 0.61 0.67 0.44 1.69 1.36 1.94 0.03 1.06 enrichments (2 ≤ EF < 5) were found: For Cd (sample S−4) and Zn (sample S−6) in summer, that can 3.2. Contamination Degree Assessment S3 0.73 0.74 0.49 1.11 0.34 1.30 2.73 1.73 0.01 0.94 S4 0.99 1.07 0.63 0.64 0.47 1.78 1.42 2.22 0.03 1.04 Pollution indices may help to quantify the level of enrichment of a sediment with trace metals S5 0.72 0.65 0.48 0.84 0.49 1.47 2.51 1.12 0 0.69 [63]. The EFs calculated on the dataset are reported in Table 3. All the stations showed no enrichments S6 1.40 1.16 0.69 0.60 0.55 2.52 1.95 1.41 0.01 0.84 (EF < 2) for V, Cr, Co, Ni, Cu, Hg, and Pb, both concerning the summer and the autumn, thus S1 0.46 0.48 0.35 0.81 0.24 0.87 2.85 1.02 0 0.70 indicating concentration levels c S2 0.85 lose to t 0.84 he natura 0.58 l backgroun 0.64 d for 0.49 marine 1.60sedim 1.48 ents [55]. Moderate 1.87 0.03 1.02 S3 0.66 0.59 0.43 0.76 0.32 1.21 2.56 1.47 0.01 0.83 enrichments (2 ≤ EF < 5) were found: For Cd (sample S−4) and Zn (sample S−6) in summer, that can S4 0.85 0.90 0.54 0.79 0.51 1.61 1.60 1.84 0.03 0.84 be linked to the upwelling phenomenon of cold water masses on the southern Atlantic coasts of S5 0.66 0.61 0.47 0.81 0.36 1.32 2.78 1.49 0.01 0.82 Morocco. Our findings corroborate with results of Idardare et al. [64] using Nereis diversicolor as S6 1.08 1.08 0.61 0.58 0.43 1.77 2.02 1.19 0.04 0.92 bio−indicator; for As in both the seasons (S−1, S−3, S−5 in summer and S−1, S−3, S−5, S−6 in autumn), Average 0.81 0.79 0.52 0.75 0.40 1.49 2.13 1.51 0.02 0.86 that can be attributed to the li Bold: autumn thol season; ogy of thi italic: s summer area aseason. nd especially to the presence of schist rocks [64,65]. On the whole, EF index values showed the following trend in summer As > Zn > Cd > Pb > V > Cr > 3.3. Correlation Matrix Ni > Co > Cu > Hg and the following one in autumn As > Cd > Zn > Pb > V > Cr > Ni > Co > Cu > Hg. The Spearman’s correlation coefficients for Al, the OM and the studied metals (Al nor- Nevertheless, according to Atgin et al. [66], EF values less than 5 cannot be considered noteworthy, malized) are presented in Table 4. According to the results, some trace metals (especially V, and a minor enrichment can be related to the effect of the lithology. The Igeo results are summarized Cr, Co, Zn, and Pb) show a very good correlation with the OM, Al, clay, and silt, indicating in Figure 5, showing that all the sampling stations can be classified as “uncontaminated” for the that their distribution in the samples is mainly controlled by the OM and the sediment’s studied trace metals (Igeo < 0). The PLI values for all the samples ranged from 0.07 to 0.43, with fine fraction; moreover, this evidence suggests that the geogenic source of these trace averages of 0.22 and 0.20 in summer and autumn, respectively. The PLI trends were as follow: S−4 > element is still predominant over anthropogenic sources. S−2 > S−6 > S−5 > S−3 > S−1 in summer, and S−6 > S−2 > S−4 > S−5 > S−3 > S−1 in autumn (Figure 6). This index supports again the evidence that no appreciable input from anthropogenic sources affects the area (PLI < 1). Earth 2021, 2 26 Earth 2020, 1, FOR PEER REVIEW 13 (a) (b) Earth 2020, 1, FOR PEER REVIEW 14 Figure 5. The Index of geo−accumulation (Igeo) of metals in Khnifiss sediments: (a) Summer season; Figure 5. The Index of geoaccumulation (Igeo) of metals in Khnifiss sediments: (a) Summer season; (b) autumn season. (b) autumn season. Figure 6. Pollution Load Index (PLI) for all sediments from Khnifiss Lagoon. Figure 6. Pollution Load Index (PLI) for all sediments from Khnifiss Lagoon. 3.3. Correlation Matrix 3.4. Comparison with Other Studies The Spearman’s correlation coefficients for Al, the OM and the studied metals (Al normalized) The overall results were compared with the data published in a selection of are presented in Table 4. According to the results, some trace metals (especially V, Cr, Co, Zn, and papers focused on lagoon areas of Morocco and other countries around the world Pb) show a very good correlation with the OM, Al, clay, and silt, indicating that their distribution in (Table 5) [7,58,59,64,67–75]. With the exclusion of the data from Rumisha et al. [68] at the samples is mainly controlled by the OM and the sediment’s fine fraction; moreover, this evidence Dar es Salam coast (Tanzania), the amounts of trace metals at Khnifiss Lagoon were lower suggests that the geogenic source of these trace element is still predominant over anthropogenic than the ones found in the other sites. Furthermore, a comparison of our results with sources. previous studies in the Khnifiss area reveals that the levels of all trace metals, except for Pb and Zn, are two times lower than those reported by Idardare et al. [64]. 3.4. Comparison with Other Studies The overall results were compared with the data published in a selection of papers focused on lagoon areas of Morocco and other countries around the world (Table 5) [7,58,59,64,67–75]. With the exclusion of the data from Rumisha et al. [68] at Dar es Salam coast (Tanzania), the amounts of trace metals at Khnifiss Lagoon were lower than the ones found in the other sites. Furthermore, a comparison of our results with previous studies in the Khnifiss area reveals that the levels of all trace metals, except for Pb and Zn, are two times lower than those reported by Idardare et al. [64]. Earth 2021, 2 27 Table 4. Spearman’s correlation matrix in Khnifiss Lagoon sediments. Al V Cr Co Ni Cu Zn As Cd Hg Pb pH OM CaCO Clay Silt Sand Al - 0.66 0.66 0.66 0.77 0.49 0.77 0.89 0.66 0.83 0.60 0.77 0.77 0.89 0.83 0.83 0.83 V 1.00 - 1.00 1.00 0.77 0.66 0.94 0.54 0.66 0.62 0.60 0.77 0.77 0.89 0.83 0.83 0.83 Cr 0.94 0.94 - 1.00 0.77 0.66 0.94 0.54 0.66 0.62 0.60 0.77 0.77 0.89 0.83 0.83 0.83 Co 1.00 1.00 0.94 - 0.77 0.66 0.94 0.54 0.66 0.62 0.60 0.77 0.77 0.89 0.83 0.83 0.83 Ni 0.71 0.71 0.60 0.71 - 0.54 0.83 0.77 0.31 0.62 0.26 0.66 0.54 0.77 0.60 0.60 0.60 Cu 0.77 0.77 0.83 0.77 0.37 - 0.83 0.20 0.14 0.83 0.09 0.14 0.31 0.71 0.37 0.37 0.37 Zn 0.94 0.94 1.00 0.94 0.60 0.83 - 0.60 0.54 0.83 0.49 0.66 0.71 0.94 0.77 0.77 0.77 As 0.77 0.77 0.71 0.77 0.66 0.89 0.71 - 0.54 0.62 0.60 0.77 0.77 0.66 0.71 0.71 0.71 Cd 0.24 0.24 0.24 0.24 0.00 0.72 0.24 0.72 - 0.41 0.94 0.89 0.89 0.71 0.94 0.94 0.94 Hg 0.88 0.88 0.88 0.88 0.68 0.88 0.88 0.88 0.41 - 0.41 0.41 0.62 0.83 0.62 0.62 0.62 Pb 0.89 0.89 0.77 0.89 0.83 0.77 0.77 0.94 0.48 0.88 - 0.83 0.94 0.60 0.89 0.89 0.89 pH 0.71 0.71 0.49 0.71 0.77 0.37 0.49 0.66 0.24 0.49 0.83 - 0.89 0.77 0.94 0.94 0.94 OM 0.89 0.89 0.77 0.89 0.83 0.77 0.77 0.94 0.48 0.88 1.00 0.83 - 0.77 0.94 0.94 0.94 CaCO 0.94 0.94 1.00 0.94 0.60 0.83 1.00 0.71 0.24 0.88 0.77 0.49 0.77 - 0.89 0.89 0.89 Clay 0.83 0.83 0.77 0.83 0.94 0.60 0.77 0.77 0.12 0.88 0.89 0.66 0.89 0.77 - 1.00 1.00 Silt 0.89 0.89 0.77 0.89 0.83 0.77 0.77 0.94 0.48 0.88 1.00 0.83 1.00 0.77 0.89 - 1.00 Sand 0.94 0.94 0.89 0.94 0.89 0.71 0.89 0.83 0.24 0.88 0.94 0.77 0.94 0.89 0.94 0.94 - Bold: autumn season; italic: summer season. Table 5. Metal concentrations (mg/kg), as dry weight, measured for the present study (ranges and mean) and comparison with other lagoon areas around the world. Country Location V Cr Co Ni Cu Zn As Cd Hg Pb References 10.9–76.8 7.50–57.8 1.21–7.13 9.95–26.1 1.95–12.6 15.0–101 6.48–13.2 0.06–0.40 <0.005–0.01 2.52–12.4 Morocco Khnifiss Lagoon This study (38.8) (26.6) (3.57) (16.5) (6.60) (51.7) (8.50) (0.16) (0.01) (6.13) Khnifiss Lagoon - 62 - 35 17 70 - 0.37 - 6 [64] Moulay Bousselham Lagoon - 19–113 - 11–96 22–311 167–759 - 0.02–0.84 0.02–0.61 6.2–32 [58] Oualidia Lagoon - 52.48 - - 36.46 227.86 - - 0.66 54.59 [59] Nador Lagoon 85 55 15 26 36 98 14 1 - 52 [67] Oualidia Lagoon 76.9 102.4 13.1 15.73 17.7 75.8 10.9 0.66 - 10.1 [72] Italy Bagnoli 100 39.8 - 12.6 26.4 287 41.6 0.46 0.35 121 [7] Tanzania Dar es Salaam Coast 4.55 4.28 0.76 1.12 0.83 5.75 0.71 0.217 - 1.21 [68] Puerto Rico Joyuda Lagoon - - - - 22 52 18 0.10 0.17 7.6 [69] San Jose Lagoon - - - - 105 531 13.3 1.8 1.9 219 [69] Iran Chabahar Bay, Oman Sea 47.21 185.01 8.91 40.51 14.16 37.86 13.22 - - 12.87 [70] Saudi Arabia Al Kharrar Lagoon - 20.62 4.77 8.67 16 39.71 1.67 0.26 0.01 50.87 [71] Bangladesh Sangu River Estuary - 25.15 - 32.75 29.24 88.97 2.58 - - 19.58 [73] Turkey Çardak Lagoon 43.6 55.6 12.0 57.9 32.9 65.3 18.6 0.2 0.046 18.6 [74] Australia Narrabeen Lagoon - 21 5.0 11 16 103 - - - 47 [75] Earth 2021, 2 28 4. Conclusions In this study, 10 trace metals (V, Cr, Co, Ni, Cu, Zn, As, Cd, Hg, and Pb) in surface sediments from Khnifiss Lagoon (Morocco) were examined and compared to the values found in the lagoon sediments from other countries and with the literature background data. The results, including correlations and a selection of contamination indices, suggested that the trace element contents in Khnifiss Lagoon are of natural origin and not significantly affected by anthropogenic sources. The current status of the area is also related to the limited access to the ecosystem, denoting a good example of wetland management. Our data represent only a preliminary screening that can be used as a starting point for a more detailed evaluation of the background levels of trace metals in the Moroccan lagoon areas. Further investigations are in progress on the site, including the study of the trace metals temporal trend. Due to the presence of low concentrations of trace metals, periodic monitoring campaigns of the lagoon are required, in order to highlight any possible early- stage contamination event and to limit/reduce any negative effects utilizing the best environmental management practices available. Author Contributions: Conceptualization, A.T.; investigation, A.T., R.C., C.C., M.D.C., E.N. and M.R.M.; validation, E.N. and M.R.M.; formal analysis, A.T. and L.P.; data curation, A.T. and L.P.; writing— original draft preparation, A.T. and F.S.; writing—review and editing, F.S. and M.P.; supervision, M.A., G.A. and B.Z. All authors have read and agreed to the published version of the manuscript. Funding: This research received no external funding. Acknowledgments: The first author (A.T.) is deeply thankful to the ENEA laboratory (Italy) for providing all necessary research facilities, and greatly acknowledges the receipt of a fellowship from the ICTP Programme for Training and Research in Italian Laboratories, Trieste, Italy. The authors thank also the “Haut Commissariat aux Eaux Et Forêts et à la Lutte Contre la Désertification” for giving access during the sampling campaign. They are also delighted to express their gratitude and sincere thanks to the editor and the reviewers for their useful comments and suggestions to improve the quality of this article. Conflicts of Interest: The authors declare no conflict of interest. References 1. Mitsch, W.J.; Gosselink, J.G. Wetlands, 5th ed.; John Wiley & Sons, Inc.: Hoboken, NJ, USA, 2015. 2. Van Den Broeck, M.; Waterkeyn, A.; Rhazi, L.; Grillas, P.; Brendonck, L. Assessing the ecological integrity of endorheic wetlands, with focus on Mediterranean temporary ponds. Ecol. 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Assessment of Trace Metals in Sediments from Khnifiss Lagoon (Tarfaya, Morocco)

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Article Assessment of Trace Metals in Sediments from Khnifiss Lagoon (Tarfaya, Morocco) 1 , 2 2 2 2 Ali Tnoumi * , Massimo Angelone , Giovanna Armiento , Raffaela Caprioli , Cinzia Crovato , 2 2 2 , † 3 2 Maurizio De Cassan , Maria Rita Montereali , Elisa Nardi , Luisa Parrella , Marco Proposito , 2 1 Fabio Spaziani and Bendahhou Zourarah LGMSSURAC45, Faculty of Sciences, Chouaib Doukkali University, El Jadida 24000, Morocco; zourarah.b@ucd.ac.ma ENEA C.R. Casaccia, Department for Sustainability, 00123 Rome, Italy; massimo.angelone@enea.it (M.A.); giovanna.armiento@enea.it (G.A.); raffaela.caprioli@enea.it (R.C.); cinzia.crovato@enea.it (C.C.); maurizio.decassan@enea.it (M.D.C.); mariarita.montereali@enea.it (M.R.M.); elisa.nardi@isprambiente.it (E.N.); marco.proposito@enea.it (M.P.); fabio.spaziani@enea.it (F.S.) ENEA C.R. Portici, Department for Sustainability, Portici, 00196 Naples, Italy; luisa.parrella@enea.it * Correspondence: tnoumi.ali@gmail.com or tnoumi.a@ucd.ac.ma; Tel.: +212-664-649-183 † Now working at ISPRA—The Italian National Institute for Environmental Protection and Research, 00144 Rome, Italy. Abstract: Surface sediments from Khnifiss lagoon (Morocco) were analyzed to evaluate the con- tamination degree of the area. Concentrations of V, Cr, Co, Ni, Cu, Zn, As, Cd, Hg, and Pb were determined on samples taken during the summer and the autumn of 2016. On the whole, higher concentrations were found in the summer season. The results revealed the following average con- centrations (mg/kg), reported in descending order: Zn (51.7  31.3) > V (38.8  24.7) > Cr (26.6 17.8) > Ni (16.5  5.47) > As (8.50  2.00) > Cu (6.60  3.81) > Pb (6.13  3.46) > Co (3.57  2.09) > Cd (0.16  0.11) > Hg (0.006  0.001). Organic matter showed a positive significant correlation with some trace metals (mainly V, Cr, Co, Zn, Cd, Pb). Three pollution indices were calculated: Citation: Tnoumi, A.; Angelone, M.; Enrichment Factor (EF), Index of Geoaccumulation (Igeo), and Pollution Load Index (PLI). Minimal Armiento, G.; Caprioli, R.; Crovato, enrichments (for Zn, As, and Cd) were detected at some sampling points. Overall indices showed C.; De Cassan, M.; Montereali, M.R.; that the Khnifiss sediments can be classified as not contaminated, and that the trace metals amounts Nardi, E.; Parrella, L.; Proposito, M.; found are ascribable to the geogenic origin. The results of this work can be used as a starting point et al. Assessment of Trace Metals in for further evaluations of trace metals distribution in Moroccan lagoons. Sediments from Khnifiss Lagoon (Tar- faya, Morocco). Earth 2021, 2, 16–31. Keywords: Khnifiss Lagoon; trace metals; enrichment factor; sediment geochemistry; contamina- https://dx.doi.org/10.3390/earth201 tion assessment Received: 19 November 2020 Accepted: 7 December 2020 1. Introduction Published: 23 December 2020 Coastal lagoons are very important environments, both in ecological and in economic Publisher’s Note: MDPI stays neu- terms, since they provide the habitat for numerous species and are the backbone of various tral with regard to jurisdictional claims anthropic activities (including food-related ones and tourism) [1,2]. However, the human in published maps and institutional exploitation of these ecosystems implies a significant potential environmental vulnerability, affiliations. especially due to their geo-morphological setting (as semi-enclosed area) and their link to terrestrial inputs and continental run-off [3,4]. Indeed, the presence of trace metals in lagoons and coastal zones has been attributed mutually to natural events (volcanism, flash flood, wind transport, erosion) and anthropogenic processes (vehicle emission, in- Copyright: © 2020 by the authors. Li- dustry, urbanization, construction activities, mining activities) [5–8]; for these reasons, censee MDPI, Basel, Switzerland. This lagoon sediments can act both as geogenic source and as a final sink for trace metals in article is an open access article distributed the aquatic ecosystems [9,10]. They can be considered as good geo-indicators of marine under the terms and conditions of the environmental quality, since their status can alter benthos and the food chain, thus posing Creative Commons Attribution (CC BY) potential ecological risks [11,12]. Therefore, the study of coastal sediments provides useful license (https://creativecommons.org/ licenses/by/4.0/). information on marine pollution [13]. Trace metals are persistent in the environment and Earth 2021, 2, 16–31. https://dx.doi.org/10.3390/earth2010002 https://www.mdpi.com/journal/earth Earth 2021, 2 17 are subject to bioaccumulation (which often causes irreversible adverse effects on ani- mals and humans) [14,15]. In addition, they represent a global-scale environmental issue, since serious problems caused by this category of pollutants are known to exist in many areas of the world (both in developed and developing countries) [16,17]. More than 90% of trace metals load in aquatic systems is associated with suspended particulate matter and sediments [18,19]. The fate and transport of these elements in marine area and lagoons are controlled by a variety of factors such as redox potential, texture, ionic strength, the abun- dance of adsorbing surfaces, pH, and organic matter content [20,21]. The environmental monitoring helps to develop effective management plans, to improve the strategies for coastal activities, and to distinguish between the anthropogenic and the natural sources of the trace metal enrichments. Morocco acts as an especially relevant site for the study of coastal ecosystems, since it holds five lagoons: Four facing the Atlantic Ocean (Khnifiss, Sidi Moussa, Oualidia, and Moulay Bousselham lagoons) and one facing the Mediterranean Sea (Marchica La- goon), covering about 3500 Km of the national coastline. The present study focuses on the most important desert wetland in the Saharan coast of Morocco and of North Africa (RAMSAR site since 1980): The lagoon of Khnifiss. It is a shallow tidal lagoon of open water in an arid environment, where the supply of continental material is strongly lim- ited to aeolian particle inputs; in addition, a coastal upwelling from the Canary Islands induces high biogenic accumulation rates [22]. Although Khnifiss Lagoon is currently protected, information on its sediment’s metal concentrations is still lacking. The relevance of Khnifiss Lagoon is further strengthened considering that there is a proposal to use the area as a reference site for the evaluation of trace metal contamination levels, since it is characterized by abundant and various macrobenthic fauna [23], low density of human population (1.07 per/km at Tarfaya Province) [24], and it is far from major cities and industrial activities. The main objectives of this research are to determine the trace metal contents in the Khnifiss Lagoon, in order to assess the contamination degree of the area; to carry out a preliminary screening that can be used as starting-point for a more detailed activity of geochemical study. 2. Materials and Methods 2.1. GeoEnvironmental Setting of the Area The study area is located in the Province of Tarfaya, between Cap Nachtigal and Cap Juby, in a rural region on the Southwest side of Morocco (27.80–28.05 N, 12.21–12.39 W). It extends for about 20 km, with a maximum of about 5 km width, at Sabkha Tazera [25]. The site is considered one of the most important zones for birds’ migrations. Besides, it provides the livelihood of people living in adjacent areas, where most activities are related to fishing and nature tourism. The lagoon is connected to the Atlantic Ocean through Foum Agoutir at its Northern side. The tidal regime is semidiurnal, and it ranges between a minimum of 1.48 m down- stream to a maximum of 2.54 m inside the lagoon. The speed of the tidal current exceeds 91 cm/s at Foum Agoutir. The residence time of the water in the lagoon is 4.4 tidal cycles during neap tides and 1.5 tidal cycles during spring tides [26]. Salinity is between 30‰ and 40‰ in the downstream zone, between 32‰ and 45‰ in the intermediate zone, and be- tween 37‰ and over 45‰ in the upstream zone due to the saltworks at Sebkha Tazera [27]. The phytoplankton community is dominated by diatoms (90%) and dinoflagellates (10%), with Chaetoceros (88.5%) and Amphora (6.7%) being the main exponents of the popula- tion [28]. The lagoon has varieties of floral species such as Zygophyllum geatulum, Launaea arborescens, Lycium intricatum, Cistanche phelypaea, Zostera noltii, and Spartina maritima [29]; seaweeds are mainly represented by 24 species (4 Chlorophyceae, 10 Phaeophyceae, and 10 Rhodophyceae) [30]. The air temperature ranges between a minimum of 15.8  2.5 C to a maximum of 24.0  2.2 C with an average of 19.9  2.2 C. The precipitations are classified as extremely rare, with an average of 0.3  0.2 mm daily and 92.6  58.5 mm annually Earth 2021, 2 18 over the last three decades (1994–2019). The wind speed ranges between 4.47  0.53 m/s to 7.90  0.82 m/s, with an average of 6.13  0.41 m/s. Earth 2020, 1, FOR PEER REVIEW 3 The geomorphology of the region is conditioned by the presence of Cretaceous marl or marly-limestone, to which it is over-imposed a harder calcareous-sandstone (Marine The geomorphology of the region is conditioned by the presence of Cretaceous marl or marly- Moghrebien) protective slab. From a geological point of view (Figure 1), the study area limestone, to which it is over-imposed a harder calcareous-sandstone (Marine Moghrebien) is located in the North-Eastern side of Tarfaya coastal basin, formed during the Mesozoic protective slab. From a geological point of view (Figure 1), the study area is located in the North- and the Cenozoic seaward of the stable West African craton [31]. The main sources of the Eastern side of Tarfaya coastal basin, formed during the Mesozoic and the Cenozoic seaward of the sediments are the AntiAtlas in the Est, and the Reguibat dorsal and Mauritanides in the stable West African craton [31]. The main sources of the sediments are the Anti−Atlas in the Est, and Southern part [32,33]. the Reguibat dorsal and Mauritanides in the Southern part [32,33]. Figure 1. Geological map of the area surrounding the Khnifiss Lagoon (Digitalized from [34]). Figure 1. Geological map of the area surrounding the Khnifiss Lagoon (Digitalized from [34]). 2.2. Sediment Sampling and Analysis 2.2. Sediment Sampling and Analysis SurSurface face sedisediments ments werewer collect e collec ed in ted 201 in 6, 2016, during during two se two asonseasons s (summ(summer er and autand umnautumn), ), at six different stations (S1–S6), for a total of 12 samples, ensuring spatial coverage of the lagoon (Figure 2). at six different stations (S1–S6), for a total of 12 samples, ensuring spatial coverage of The sediments were taken using a stainless steel Van Veen grab sampler, between 0–5 cm depth. The the lagoon (Figure 2). The sediments were taken using a stainless steel Van Veen grab tools were washed with lagoon water before sampling. The sediments were stored in airtight sampler, between 0–5 cm depth. The tools were washed with lagoon water before sampling. polyethylene bags, labeled, and immediately frozen at −20 °C until further processing. The location The sediments were stored in airtight polyethylene bags, labeled, and immediately frozen of each samp  le was recorded by a GPS. At the laboratory, the sediments were defrosted by keeping at 20 C until further processing. The location of each sample was recorded by a GPS. Earth 2021, 2 19 At the laboratory, the sediments were defrosted by keeping them at room temperature for about 24 h, then dried in an oven at 40 C (it has been proved by Gilli et al. [35] that samples drying at 40 C does not cause evaporation losses of Hg). The samples were at last crushed using a mortar grinder, homogenized, stored inside polyethylene bags until the chemical analyses were carried out. Earth 2020, 1, FOR PEER REVIEW 5 Figure 2. Map of the sampling locations at Khnifiss Lagoon. Figure 2. Map of the sampling locations at Khnifiss Lagoon. Earth 2021, 2 20 The determinations of trace metals (except Hg) were accomplished using a microwave- assisted acid dissolution procedure, according to EPA method 3052, followed by the chemical analyses performed by: (i) An ICP-MS instrument (Perkin Elmer ELAN 6100) for the quantification of V, Cr, Co, Ni, Cu, Zn, As, Cd, and Pb (according to EPA method 6020 b); (ii) an ICPOES instrument (Perkin Elmer Optima2000 DV) for Al (according to EPA method 6010 d). All the reagents were of analytical grade. The analysis of Hg was carried out by the automated mercury analyzer FKV AMA-254; the advantage of this device is that Hg is directly determined on the dry sample and the acid dissolution step is not required [36]. Precision and accuracy were verified using marine sediment reference certified materials (PACS-3 and MESS-4; National Research Council Canada, NRCC): The recoveries of Al, V, Cr, Co, Ni, Cu, Zn, As, Cd, Hg, and Pb in MESS4 ranged from 84.0 to 118.5%; the recoveries of Al, V, Cr, Co, Ni, Cu, Zn, As, Cd, and Pb in PACS3 ranged from 88.1 to 100.5%. For all the elements, the quantitation limits were: V, Co, As, Pb = 0.05 mg/kg; Cd = 0.03 mg/kg; Cr = 0.5 mg/kg; Ni, Cu = 0.3 mg/kg; Zn = 1.0 mg/kg; Hg = 0.005 mg/kg; Al = 50 mg/kg. Organic matter (OM), which in sea sediments originates from marine and terrestrial sources, was determined by LossOnIgnition procedure (LOI) [37]. About 4 g of each sediment were weighed and transferred in a porcelain crucible. The samples were heated at 105 C for 24 h to remove the moisture content and the new weight was determined; then, the samples were kept in a muffle furnace at 550 C for 4 h and weighed again. Lastly, the organic matter was calculated according to Equation (1): Weight Weight 105 C 550 C LOI =   100 (1) Weight 105 C The calcium carbonate content (CaCO ) was measured by volumetric calcimetry, using a Bernard calcimeter [38,39]. Grain size measurement was determined using both dry and wet separation techniques [40–42]: The samples were pre-treated with H O and 2 2 washed with bidistilled water to remove organic matter and chlorides, then oven-dried at 40 C for 24 h; the coarse fraction was isolated by sieving, while the fine fraction (<63 m) was ultrasonically dispersed using 0.5~10% sodium hexametaphosphate (NaPO ) for 24 h 3 6 and analyzed by a particle size analyzer (Micromeritics, SediGraph). 2.3. Pollution Indices To assess the levels of trace metal contamination and the possible anthropogenic impact on the sediments, three of the most used pollution indices were applied [43,44]: Enrichment Factor (EF), Index of geo-accumulation (Igeo), and Pollution Load Index (PLI). The calculations of the indices require the use of reference values from a background matrix, and the Shale data proposed by Turekian and Wedepohl [45] were chosen for this study (because local geochemical background values were not available). The EF is used to evaluate the degree of anthropogenic influence on the occurrence of a metal in the soil/sediment; it is calculated through the ratio of the metal concentration in the sample with respect to the background matrix, using a normalizing element [46]. Several conservative elements have been used for normalization purposes [47–49], but we selected Al since it is generally the major constituent of clay minerals. The EF for a trace metal is expressed as follow (Equation (2)): (C /C )Sample n Al EF = (2) (C /C )Background Al where C and C are the concentrations of the trace metal (n) and Al. n Al Earth 2021, 2 21 The Igeo index, originally developed by Muller [50], was used to quantify the metal contamination related both to natural events and/or human activities [51]. It is calculated according to Equation (3): Igeo = log (3) k  B where C is the concentration of the metal (n) in the sample and B is the geochemical n n background value of the same element [52]. The constant k = 1.5 is used as a correction coef- ficient for natural fluctuations in background values as well as for very small anthropogenic influences [50]. PLI provides an integrated and site wise comparative assessment of cumulative trace metal pollution [53]. This index can be interpreted as an appraisal of the comprehensive trace metal pollution in a given sample. It is calculated using the Equations (4) and (5): PLI = (CF )  (CF )  (CF )  . . .  (CF ) (4) 2 3 n CF = C /C (5) Sample Background where CF is the contamination factor, C is the concentration of a selected metal in Sample the sample, and C is the amount of the same element in the background matrix. Background Each index evaluates the sample quality according to the scores and classes [53–55] reported in Table 1. Table 1. Scores and classes of Pollution Load index (PLI), Index of geoaccumulation (Igeo), and Enrichment Factor (EF). PLI [53] Degree of Contamination Igeo [54] Degree of Contamination EF [55] Degree of Contamination <1 Unpolluted Igeo  0 Uncontaminated EF < 2 No enrichment/Depletion to mineral >1 Polluted 0 < Igeo  1 Uncontaminated to moderately 2  EF< 5 Moderate enrichment 1 < Igeo  2 Moderately contaminated 5  EF < 20 Significant enrichment 2 < Igeo  3 Moderately to highly contaminated 20  EF < 40 Very high enrichment 3 < Igeo  4 Highly contaminated EF  40 Extremely enrichment 4 < Igeo  5 Highly to extremely contaminated Igeo > 5 Extremely contaminated 2.4. Correlation Analysis The data processing was performed using R statistical software [56]. Mean, maximum, minimum, standard deviation, first quartile, third quartile, and Skewness were calculated. The Shapiro-Wilk test was used to assess the normality of the dataset. Since the data were not normally distributed, the Spearman correlation coefficient was used in order to identify any significant pattern and relationship between the variables. 3. Results and Discussion 3.1. Geochemical Results The complete dataset and the descriptive statistics are summarized in Table 2. The sed- iments of the lagoon showed a moderately alkaline pH, which ranged from 7.55 to 8.27, and exhibited spatial differences in the occurrence of trace metals (Figure 3). Stations S–2, S–4, and S–6 revealed higher concentrations compared to other locations, probably due to different grain size distribution; these samples, indeed, may have a larger accumulation of fine grained terrigenous and marine materials as reflected by their higher amounts of Al. In the summer season, station S–4 stands out for the higher concentrations exhibited for all the elements. In autumn, instead, S–6 has higher concentrations of V, Cr, Co, Cu, Zn, As, Hg, and Pb, while S–2 and S–4 had the maximum amounts of Ni and Cd. The OM in sediments is a key parameter since it is related to the ability to concentrate (either by ion exchange and/or complexation) and release metals in marine and lagoon waters, and it is also a source of nutrients for aquatic fauna [57]. It ranged between 1.50 and 7.56%, with an average of 3.78% (Figure 4). The lowest amounts were observed, in both seasons, at S–1 (Foum Agoutir, inlet of the lagoon), due to high hydrodynamic characteristic Earth 2021, 2 22 at this station and for the prevalence of sandy texture [26]. Generally, sediments in rough waters are sandy and then exhibit lower metal contents [47]. On the contrary, the maximum amounts of OM, that speed up the adsorption of the metals from the overlying water to bottom sediments, were recorded at station S–2 and S–4 due to the presence of seagrass, which encourages the retention of the fine particles. The relatively higher concentrations observed during the summer season for most of the trace metals may be attributed to the small differences in OM contents, having an average of 3.92  2.63% compared to the 3.65  2.28% in autumn. The lower OM in fall may be a consequence of elevated decomposition rates fostered by high levels of dissolved oxygen [27]. However, the lower metal concentrations found during the autumn might be also related to their mobilization from the sediments promoted by the overlying water. The ranges of OM percentages were compared to other Moroccan Lagoons, e.g., Moulay Bousselham Lagoon (1.24–15.74%) [58] and Oualidia lagoon (7.56–15.23%) [59]. The comparison showed that the OM values in the Khnifiss Lagoon sediments are about two times lower than the ones from the other local lagoons. This could justify the low concentration values of trace metals measured in this lagoon. Lefrere et al. [23] achieved the same conclusion using benthic fauna. In addition, the lagoon receives neither freshwater supply from the mainland nor waste discharges, and therefore is characterized only by a continuous renewal of water according to the current tidal trend [26]. The carbonate concentrations varied in a range of 8.25–58.8% and the highest values were registered, in both seasons, in stations S1 and S3. These results are reasonably related to the nature of the sediments since the presence of limestone fragments (from shells and snails) causes the rise of carbonate contents. The importance of determining and studying carbonates in marine systems is related to their ability to adsorb metal forming labile species [60]. Overall, the means of the analyzed elements are below the average shale’s values [45,61,62] (Table 2) indicating low metal concentrations in the lagoon sediments (Figure 2). The world average shale value represents the background concentration of metals in a collection of several sedimentary rocks derived from argillaceous materi- als worldwide. 3.2. Contamination Degree Assessment Pollution indices may help to quantify the level of enrichment of a sediment with trace metals [63]. The EFs calculated on the dataset are reported in Table 3. All the stations showed no enrichments (EF < 2) for V, Cr, Co, Ni, Cu, Hg, and Pb, both concerning the summer and the autumn, thus indicating concentration levels close to the natural background for marine sediments [55]. Moderate enrichments (2  EF < 5) were found: For Cd (sample S4) and Zn (sample S6) in summer, that can be linked to the upwelling phenomenon of cold water masses on the southern Atlantic coasts of Morocco. Our findings corroborate with results of Idardare et al. [64] using Nereis diversicolor as bioindicator; for As in both the seasons (S1, S3, S5 in summer and S1, S3, S5, S6 in autumn), that can be attributed to the lithology of this area and especially to the presence of schist rocks [64,65]. On the whole, EF index values showed the following trend in summer As > Zn > Cd > Pb > V > Cr > Ni > Co > Cu > Hg and the following one in autumn As > Cd > Zn > Pb > V > Cr > Ni > Co > Cu > Hg. Nevertheless, according to Atgin et al. [66], EF values less than 5 cannot be considered noteworthy, and a minor enrichment can be related to the effect of the lithology. The Igeo results are summarized in Figure 5, showing that all the sampling stations can be classified as “uncontaminated” for the studied trace metals (Igeo < 0). The PLI values for all the samples ranged from 0.07 to 0.43, with averages of 0.22 and 0.20 in summer and autumn, respectively. The PLI trends were as follow: S4 > S2 > S6 > S5 > S3 > S1 in summer, and S6 > S2 > S4 > S5 > S3 > S1 in autumn (Figure 6). This index supports again the evidence that no appreciable input from anthropogenic sources affects the area (PLI < 1). Earth 2021, 2 23 Table 2. Geochemical analysis results and descriptive statistics in surface sediments from Khnifiss Lagoon. Al V Cr Co Ni Cu Zn As Cd Hg Pb OM CaCO Clay Silt Sand pH % mg/kg mg/kg mg/kg mg/kg mg/kg mg/kg mg/kg mg/kg mg/kg mg/kg % % % % % S1 1.79 11. 5 7.50 1.36 12.3 2.23 15.9 6.70 0.06 <0.005 2.89 8.20 1.80 54.5 0.03 0.80 99.2 S2 4.05 60.7 44.3 5.90 22.9 10.1 81.1 8.92 0.29 0.01 10.8 7.68 7.36 29.6 20.4 45.5 34.1 S3 1.46 17.4 12.2 1.71 13.8 2.79 22.5 6.48 0.10 <0.005 3.44 7.89 2.25 51.9 1.80 2.80 95.4 S4 4.78 76.8 57.8 7.13 26.1 12.6 101 11.1 0.40 0.01 12.4 7.59 7.16 13.3 22.4 58.6 19.1 S5 2.10 24.6 15.4 2.40 15.0 5.77 36.6 8.57 0.09 <0.005 3.63 8.27 1.88 33.8 0.26 1.10 98.6 S6 2.72 61.9 35.5 4.47 13.8 8.38 81.4 8.64 0.14 <0.005 5.71 7.82 3.07 22.4 5.54 7.82 86.6 S1 1.45 10.9 7.74 1.21 9.95 1.95 15.0 6.70 0.06 <0.005 2.52 8.24 1.50 58.8 0.24 0.62 99.1 S2 3.47 48.0 32.8 4.80 19.0 9.50 65.9 8.33 0.24 0.01 8.85 7.55 7.56 28.4 16.3 37.4 46.3 S3 1.56 16.7 10.4 1.59 10.1 2.77 22.5 6.49 0.09 <0.005 3.24 7.76 2.34 45.1 0.51 2.50 97.0 S4 3.41 47.1 34.5 4.36 23.0 9.71 65.4 8.87 0.23 0.01 7.17 7.93 3.93 23.8 10.3 21.4 68.3 S5 1.78 19.2 12.2 1.99 12.3 3.60 28.0 8.04 0.10 <0.005 3.64 7.88 1.88 32.6 0.10 0.80 99.1 S6 4.04 70.8 49.1 5.88 19.8 9.77 85.0 13.2 0.18 0.01 9.29 7.72 4.68 8.25 29.4 29.3 41.3 Minimum 1.45 10.9 7.50 1.21 9.95 1.95 15.0 6.48 0.06 0.01 2.52 7.55 1.50 8.25 0.03 0.62 19.1 Maximum 4.78 76.8 57.8 7.13 26.1 12.6 101 13.2 0.40 0.01 12.4 8.27 7.56 58.8 29.4 58.6 99.2 Mean 2.72 38.8 26.6 3.57 16.5 6.60 51.7 8.50 0.16 0.01 6.13 7.88 3.78 33.6 8.94 17.4 73.7 St.dev. 1.18 24.7 17.8 2.09 5.47 3.81 31.3 2.00 0.11 0.00 3.46 0.25 2.35 16.1 10.6 20.6 30.4 1 Quartile 1.72 17.3 11.7 1.68 12.3 2.78 22.5 6.70 0.09 0.01 3.39 7.71 1.88 23.5 0.26 1.03 45.1 3 Quartile 3.61 61.0 37.7 5.07 20.6 9.73 81.2 8.88 0.24 0.01 8.96 8.00 5.30 46.8 17.3 31.3 98.8 Ref. data: Average 8 130 90.0 19.0 68.0 45.0 95.0 13.0 0.30 0.40 20.0 - - - - - - Shale Bold: autumn season; italic: summer season. Earth 2021, 2 24 Earth 2020, 1, FOR PEER REVIEW 9 (a) (b) (c) (d) (e) (f) (g) (h) Figure 3. Cont. Earth 2020, 1, FOR PEER REVIEW 11 Earth 2021, 2 25 Earth 2020, 1, FOR PEER REVIEW 10 (g) (h) (i) (j) (i) (j) Figure 3. Spatial variations of the investigated metals during summer and autumn of the surface Figure 3. Spatial Figure 3. variations Spatial v of the ariat investigat ions of the inv ed metals estig during ated m summer etals during summer an and autumn of the d autumn of the surface surface sediments from Khnifiss sediments from Khnifiss Lagoon: (a) Vanadium; (b) Chromium; (c) Cobalt; (d) Nickel; (e) Copper; (f) Lagoon: (a)sediments from Khnifiss Vanadium; (b) Chromium; Lagoon: (c) Cobalt; (a) Va(na d)d Nickel; ium; (b() Chro e) Copper; mium; (f ( ) c Zinc; ) Cob(a gl) t; ( Arsenic; d) Nick( eh l;) (Cadmium; e) Copper; ( (i f) ) Mercury; Zinc; (g) Arsenic; (h) Cadmium; (i) Mercury; (j) Lead. Horizontal solid red line represents the Average (j) Lead. Horizontal solid red line represents the Average Shale Background value. Zinc; (g) Arsenic; (h) Cadmium; (i) Mercury; (j) Lead. Horizontal solid red line represents the Average Shale Background value. Shale Background value. Figure 4. Organic matter (%) distribution in Khnifiss Lagoon during summer and autumn. Figure 4. Organic matter (%) distribution in Khnifiss Lagoon during summer and autumn. 3.2. Contamination Degree Assessment Table 3. Enrichment Factor (EF) in the surface sediments from Khnifiss Lagoon. Pollution indices may help to quantify the level of enrichment of a sediment with trace metals [63]. The EFs calculated on the dataset are reported in Table 3. All the stations showed no enrichments V Cr Co Ni Cu Zn As Cd Hg Pb Figure 4. Organic matter (%) distribution in Khnifiss Lagoon during summer and autumn. (EF < 2) for V, Cr, Co, Ni, Cu, Hg, and Pb, both concerning the summer and the autumn, thus S1 0.39 0.37 0.32 0.81 0.22 0.75 2.30 0.86 0 0.65 indicating concentration levels close to the natural background for marine sediments [55]. Moderate S2 0.92 0.97 0.61 0.67 0.44 1.69 1.36 1.94 0.03 1.06 enrichments (2 ≤ EF < 5) were found: For Cd (sample S−4) and Zn (sample S−6) in summer, that can 3.2. Contamination Degree Assessment S3 0.73 0.74 0.49 1.11 0.34 1.30 2.73 1.73 0.01 0.94 S4 0.99 1.07 0.63 0.64 0.47 1.78 1.42 2.22 0.03 1.04 Pollution indices may help to quantify the level of enrichment of a sediment with trace metals S5 0.72 0.65 0.48 0.84 0.49 1.47 2.51 1.12 0 0.69 [63]. The EFs calculated on the dataset are reported in Table 3. All the stations showed no enrichments S6 1.40 1.16 0.69 0.60 0.55 2.52 1.95 1.41 0.01 0.84 (EF < 2) for V, Cr, Co, Ni, Cu, Hg, and Pb, both concerning the summer and the autumn, thus S1 0.46 0.48 0.35 0.81 0.24 0.87 2.85 1.02 0 0.70 indicating concentration levels c S2 0.85 lose to t 0.84 he natura 0.58 l backgroun 0.64 d for 0.49 marine 1.60sedim 1.48 ents [55]. Moderate 1.87 0.03 1.02 S3 0.66 0.59 0.43 0.76 0.32 1.21 2.56 1.47 0.01 0.83 enrichments (2 ≤ EF < 5) were found: For Cd (sample S−4) and Zn (sample S−6) in summer, that can S4 0.85 0.90 0.54 0.79 0.51 1.61 1.60 1.84 0.03 0.84 be linked to the upwelling phenomenon of cold water masses on the southern Atlantic coasts of S5 0.66 0.61 0.47 0.81 0.36 1.32 2.78 1.49 0.01 0.82 Morocco. Our findings corroborate with results of Idardare et al. [64] using Nereis diversicolor as S6 1.08 1.08 0.61 0.58 0.43 1.77 2.02 1.19 0.04 0.92 bio−indicator; for As in both the seasons (S−1, S−3, S−5 in summer and S−1, S−3, S−5, S−6 in autumn), Average 0.81 0.79 0.52 0.75 0.40 1.49 2.13 1.51 0.02 0.86 that can be attributed to the li Bold: autumn thol season; ogy of thi italic: s summer area aseason. nd especially to the presence of schist rocks [64,65]. On the whole, EF index values showed the following trend in summer As > Zn > Cd > Pb > V > Cr > 3.3. Correlation Matrix Ni > Co > Cu > Hg and the following one in autumn As > Cd > Zn > Pb > V > Cr > Ni > Co > Cu > Hg. The Spearman’s correlation coefficients for Al, the OM and the studied metals (Al nor- Nevertheless, according to Atgin et al. [66], EF values less than 5 cannot be considered noteworthy, malized) are presented in Table 4. According to the results, some trace metals (especially V, and a minor enrichment can be related to the effect of the lithology. The Igeo results are summarized Cr, Co, Zn, and Pb) show a very good correlation with the OM, Al, clay, and silt, indicating in Figure 5, showing that all the sampling stations can be classified as “uncontaminated” for the that their distribution in the samples is mainly controlled by the OM and the sediment’s studied trace metals (Igeo < 0). The PLI values for all the samples ranged from 0.07 to 0.43, with fine fraction; moreover, this evidence suggests that the geogenic source of these trace averages of 0.22 and 0.20 in summer and autumn, respectively. The PLI trends were as follow: S−4 > element is still predominant over anthropogenic sources. S−2 > S−6 > S−5 > S−3 > S−1 in summer, and S−6 > S−2 > S−4 > S−5 > S−3 > S−1 in autumn (Figure 6). This index supports again the evidence that no appreciable input from anthropogenic sources affects the area (PLI < 1). Earth 2021, 2 26 Earth 2020, 1, FOR PEER REVIEW 13 (a) (b) Earth 2020, 1, FOR PEER REVIEW 14 Figure 5. The Index of geo−accumulation (Igeo) of metals in Khnifiss sediments: (a) Summer season; Figure 5. The Index of geoaccumulation (Igeo) of metals in Khnifiss sediments: (a) Summer season; (b) autumn season. (b) autumn season. Figure 6. Pollution Load Index (PLI) for all sediments from Khnifiss Lagoon. Figure 6. Pollution Load Index (PLI) for all sediments from Khnifiss Lagoon. 3.3. Correlation Matrix 3.4. Comparison with Other Studies The Spearman’s correlation coefficients for Al, the OM and the studied metals (Al normalized) The overall results were compared with the data published in a selection of are presented in Table 4. According to the results, some trace metals (especially V, Cr, Co, Zn, and papers focused on lagoon areas of Morocco and other countries around the world Pb) show a very good correlation with the OM, Al, clay, and silt, indicating that their distribution in (Table 5) [7,58,59,64,67–75]. With the exclusion of the data from Rumisha et al. [68] at the samples is mainly controlled by the OM and the sediment’s fine fraction; moreover, this evidence Dar es Salam coast (Tanzania), the amounts of trace metals at Khnifiss Lagoon were lower suggests that the geogenic source of these trace element is still predominant over anthropogenic than the ones found in the other sites. Furthermore, a comparison of our results with sources. previous studies in the Khnifiss area reveals that the levels of all trace metals, except for Pb and Zn, are two times lower than those reported by Idardare et al. [64]. 3.4. Comparison with Other Studies The overall results were compared with the data published in a selection of papers focused on lagoon areas of Morocco and other countries around the world (Table 5) [7,58,59,64,67–75]. With the exclusion of the data from Rumisha et al. [68] at Dar es Salam coast (Tanzania), the amounts of trace metals at Khnifiss Lagoon were lower than the ones found in the other sites. Furthermore, a comparison of our results with previous studies in the Khnifiss area reveals that the levels of all trace metals, except for Pb and Zn, are two times lower than those reported by Idardare et al. [64]. Earth 2021, 2 27 Table 4. Spearman’s correlation matrix in Khnifiss Lagoon sediments. Al V Cr Co Ni Cu Zn As Cd Hg Pb pH OM CaCO Clay Silt Sand Al - 0.66 0.66 0.66 0.77 0.49 0.77 0.89 0.66 0.83 0.60 0.77 0.77 0.89 0.83 0.83 0.83 V 1.00 - 1.00 1.00 0.77 0.66 0.94 0.54 0.66 0.62 0.60 0.77 0.77 0.89 0.83 0.83 0.83 Cr 0.94 0.94 - 1.00 0.77 0.66 0.94 0.54 0.66 0.62 0.60 0.77 0.77 0.89 0.83 0.83 0.83 Co 1.00 1.00 0.94 - 0.77 0.66 0.94 0.54 0.66 0.62 0.60 0.77 0.77 0.89 0.83 0.83 0.83 Ni 0.71 0.71 0.60 0.71 - 0.54 0.83 0.77 0.31 0.62 0.26 0.66 0.54 0.77 0.60 0.60 0.60 Cu 0.77 0.77 0.83 0.77 0.37 - 0.83 0.20 0.14 0.83 0.09 0.14 0.31 0.71 0.37 0.37 0.37 Zn 0.94 0.94 1.00 0.94 0.60 0.83 - 0.60 0.54 0.83 0.49 0.66 0.71 0.94 0.77 0.77 0.77 As 0.77 0.77 0.71 0.77 0.66 0.89 0.71 - 0.54 0.62 0.60 0.77 0.77 0.66 0.71 0.71 0.71 Cd 0.24 0.24 0.24 0.24 0.00 0.72 0.24 0.72 - 0.41 0.94 0.89 0.89 0.71 0.94 0.94 0.94 Hg 0.88 0.88 0.88 0.88 0.68 0.88 0.88 0.88 0.41 - 0.41 0.41 0.62 0.83 0.62 0.62 0.62 Pb 0.89 0.89 0.77 0.89 0.83 0.77 0.77 0.94 0.48 0.88 - 0.83 0.94 0.60 0.89 0.89 0.89 pH 0.71 0.71 0.49 0.71 0.77 0.37 0.49 0.66 0.24 0.49 0.83 - 0.89 0.77 0.94 0.94 0.94 OM 0.89 0.89 0.77 0.89 0.83 0.77 0.77 0.94 0.48 0.88 1.00 0.83 - 0.77 0.94 0.94 0.94 CaCO 0.94 0.94 1.00 0.94 0.60 0.83 1.00 0.71 0.24 0.88 0.77 0.49 0.77 - 0.89 0.89 0.89 Clay 0.83 0.83 0.77 0.83 0.94 0.60 0.77 0.77 0.12 0.88 0.89 0.66 0.89 0.77 - 1.00 1.00 Silt 0.89 0.89 0.77 0.89 0.83 0.77 0.77 0.94 0.48 0.88 1.00 0.83 1.00 0.77 0.89 - 1.00 Sand 0.94 0.94 0.89 0.94 0.89 0.71 0.89 0.83 0.24 0.88 0.94 0.77 0.94 0.89 0.94 0.94 - Bold: autumn season; italic: summer season. Table 5. Metal concentrations (mg/kg), as dry weight, measured for the present study (ranges and mean) and comparison with other lagoon areas around the world. Country Location V Cr Co Ni Cu Zn As Cd Hg Pb References 10.9–76.8 7.50–57.8 1.21–7.13 9.95–26.1 1.95–12.6 15.0–101 6.48–13.2 0.06–0.40 <0.005–0.01 2.52–12.4 Morocco Khnifiss Lagoon This study (38.8) (26.6) (3.57) (16.5) (6.60) (51.7) (8.50) (0.16) (0.01) (6.13) Khnifiss Lagoon - 62 - 35 17 70 - 0.37 - 6 [64] Moulay Bousselham Lagoon - 19–113 - 11–96 22–311 167–759 - 0.02–0.84 0.02–0.61 6.2–32 [58] Oualidia Lagoon - 52.48 - - 36.46 227.86 - - 0.66 54.59 [59] Nador Lagoon 85 55 15 26 36 98 14 1 - 52 [67] Oualidia Lagoon 76.9 102.4 13.1 15.73 17.7 75.8 10.9 0.66 - 10.1 [72] Italy Bagnoli 100 39.8 - 12.6 26.4 287 41.6 0.46 0.35 121 [7] Tanzania Dar es Salaam Coast 4.55 4.28 0.76 1.12 0.83 5.75 0.71 0.217 - 1.21 [68] Puerto Rico Joyuda Lagoon - - - - 22 52 18 0.10 0.17 7.6 [69] San Jose Lagoon - - - - 105 531 13.3 1.8 1.9 219 [69] Iran Chabahar Bay, Oman Sea 47.21 185.01 8.91 40.51 14.16 37.86 13.22 - - 12.87 [70] Saudi Arabia Al Kharrar Lagoon - 20.62 4.77 8.67 16 39.71 1.67 0.26 0.01 50.87 [71] Bangladesh Sangu River Estuary - 25.15 - 32.75 29.24 88.97 2.58 - - 19.58 [73] Turkey Çardak Lagoon 43.6 55.6 12.0 57.9 32.9 65.3 18.6 0.2 0.046 18.6 [74] Australia Narrabeen Lagoon - 21 5.0 11 16 103 - - - 47 [75] Earth 2021, 2 28 4. Conclusions In this study, 10 trace metals (V, Cr, Co, Ni, Cu, Zn, As, Cd, Hg, and Pb) in surface sediments from Khnifiss Lagoon (Morocco) were examined and compared to the values found in the lagoon sediments from other countries and with the literature background data. The results, including correlations and a selection of contamination indices, suggested that the trace element contents in Khnifiss Lagoon are of natural origin and not significantly affected by anthropogenic sources. The current status of the area is also related to the limited access to the ecosystem, denoting a good example of wetland management. Our data represent only a preliminary screening that can be used as a starting point for a more detailed evaluation of the background levels of trace metals in the Moroccan lagoon areas. Further investigations are in progress on the site, including the study of the trace metals temporal trend. Due to the presence of low concentrations of trace metals, periodic monitoring campaigns of the lagoon are required, in order to highlight any possible early- stage contamination event and to limit/reduce any negative effects utilizing the best environmental management practices available. Author Contributions: Conceptualization, A.T.; investigation, A.T., R.C., C.C., M.D.C., E.N. and M.R.M.; validation, E.N. and M.R.M.; formal analysis, A.T. and L.P.; data curation, A.T. and L.P.; writing— original draft preparation, A.T. and F.S.; writing—review and editing, F.S. and M.P.; supervision, M.A., G.A. and B.Z. All authors have read and agreed to the published version of the manuscript. Funding: This research received no external funding. Acknowledgments: The first author (A.T.) is deeply thankful to the ENEA laboratory (Italy) for providing all necessary research facilities, and greatly acknowledges the receipt of a fellowship from the ICTP Programme for Training and Research in Italian Laboratories, Trieste, Italy. The authors thank also the “Haut Commissariat aux Eaux Et Forêts et à la Lutte Contre la Désertification” for giving access during the sampling campaign. They are also delighted to express their gratitude and sincere thanks to the editor and the reviewers for their useful comments and suggestions to improve the quality of this article. Conflicts of Interest: The authors declare no conflict of interest. References 1. Mitsch, W.J.; Gosselink, J.G. Wetlands, 5th ed.; John Wiley & Sons, Inc.: Hoboken, NJ, USA, 2015. 2. Van Den Broeck, M.; Waterkeyn, A.; Rhazi, L.; Grillas, P.; Brendonck, L. Assessing the ecological integrity of endorheic wetlands, with focus on Mediterranean temporary ponds. Ecol. 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Journal

Earth – Multidisciplinary Digital Publishing Institute

Published: Dec 23, 2020

Keywords: Khnifiss Lagoon; trace metals; enrichment factor; sediment geochemistry; contamination assessment

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