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. These characteristics make the HEK293ERE/Gal4-Lux bioreporter potentially suitable as a high - throughput human cell- based assay for screening estrogenic activity. HEK293, bioreporter, autobioluminescent, endocrine ...
, respectively. Measurement of MAPK pathway activity was performed using the luciferase reporter. pERK protein levels were detected for validation . We performed a screen of a MAPKi library and calculated ...
time-consuming and expensive[26–31]. This is particularly true for drug development, since currently, high - throughput screening (HTS) of available chemical libraries is a widely used technique ...
- throughput assays for the testing of anti-Trypanosoma cruzi compounds. PLoS Negl Trop Dis 4: e740. Engel JC, Ang KK, Chen S, Arkin MR, McKerrow JH, et al. (2010) Image- based high - throughput drug screening ...
ABSTRACT The goal of this study was to develop and validate a simple but quantitative cell‐ based assay to identify compounds that might be used pharmaceutically to give tissue repair a more ...
, each with four technical replicates. Luciferase assays Luciferase assays were performed as previously described [9]. We used the firefly luciferase reporter pGL4.25 (Promega) driven by the ETS-motif ...
Beas2B as well as human primary nasal epithelial cells. Altogether, these results validate our cell- based functional assay and the interest of screening libraries of different origins to identify ...
cells leads to increased apoptosis, suggesting an anti-apoptotic function that promotes cancer progression. Yet, suitable high - throughput editing assays are needed to efficiently screen chemical libraries ...
as suitable for high - throughput drug screening [29]. We now describe the development of an efficient bioluminescent assay for measuring growth inhibition of trophozoites using an engineered G. lamblia strain ...
assay is suitable for high throughput screening of large compound libraries to identify novel allosteric inhibitors. Results Development of a conformational switch assay based on split luciferase ...
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