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The Zeta Potential and Hemagglutination with Rh Antibodies

The Zeta Potential and Hemagglutination with Rh Antibodies Int. Arch. Allergy 38: 482-496 (1970) A Physiochem ical E xplanation W. Pollack and R. P. Reckel O rth o Research Foundation, R aritan, N .J. Agglutination of red blood cells by blood group antibodies is the last of a two-stage dynamic process. It results from the linking together of red cells by the antibody molecules to form a three-dimensional lattice. Thus, any two cells of the lattice are held together by bridges of protein. For this to occur, the red cells must approach each other closely enough to be spanned by the antibody molecules. Pollack and his co-workers [6, 18] in discussing the forces involved in the second stage of hemagglutination have shown that the effective intercellular distances are a function of the £ potential of the red cells and are inversely proportional to the square root of the dielectric constant and ionic strength of the red cell suspension medium. Further evidence will be presented by the authors supporting these observations. Materials and Methods Micro-electrophoresis, dielectric constant measurements, enzyme treatm ent o f red blood cells a n d conductance measurements were performed as previously described by P ollack et at. [8] an d P ollack [7]. All http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png International Archives of Allergy and Immunology Karger

The Zeta Potential and Hemagglutination with Rh Antibodies

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Publisher
Karger
Copyright
© 1970 S. Karger AG, Basel
ISSN
1018-2438
eISSN
1423-0097
DOI
10.1159/000230301
Publisher site
See Article on Publisher Site

Abstract

Int. Arch. Allergy 38: 482-496 (1970) A Physiochem ical E xplanation W. Pollack and R. P. Reckel O rth o Research Foundation, R aritan, N .J. Agglutination of red blood cells by blood group antibodies is the last of a two-stage dynamic process. It results from the linking together of red cells by the antibody molecules to form a three-dimensional lattice. Thus, any two cells of the lattice are held together by bridges of protein. For this to occur, the red cells must approach each other closely enough to be spanned by the antibody molecules. Pollack and his co-workers [6, 18] in discussing the forces involved in the second stage of hemagglutination have shown that the effective intercellular distances are a function of the £ potential of the red cells and are inversely proportional to the square root of the dielectric constant and ionic strength of the red cell suspension medium. Further evidence will be presented by the authors supporting these observations. Materials and Methods Micro-electrophoresis, dielectric constant measurements, enzyme treatm ent o f red blood cells a n d conductance measurements were performed as previously described by P ollack et at. [8] an d P ollack [7]. All

Journal

International Archives of Allergy and Immunology – Karger

Published: Jan 1, 1970

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