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Role of Cytology and Polymerase Chain Reaction Based Detection of Pneumocystis jirovecii Infection in Bronchoalveolar Lavage Fluid

Role of Cytology and Polymerase Chain Reaction Based Detection of Pneumocystis jirovecii... Molecular Techniques Role of Cytology and Polymerase Chain Reaction Based Detection of Pneumocystis jirovecii Infection in Bronchoalveolar Lavage Fluid Rashmi Gupta, M.Sc., Venkateswaran K. Iyer, M.D., Bijay Ranjan Mirdha, M.D., Randeep Guleria, M.D., Lalit Kumar, M.D., and Sanjay Kumar Agarwal, M.D. Objective croscopy for PCP, while 5 (including 2 microscopy positive) To study the role of cytology and polymerase chain reaction were positive by PCR from BALF. Four of the 5 positive (PCR) for the detection of Pneumocystis carinii pneumo- cases showed predominantly alveolar macrophages, while nia (PCP) in bronchoalveolar lavage fluid (BALF). one showed 40% alveolar macrophages, 30% lymphocytes and 20% neutrophils upon cellular analysis of BALF. One Study Design case had coinfection with Cryptococcus. Destained smear In a prospective observational scrapings of PCR-positive study, a BALF pellet from 55 slides (n = 5) were all success- patients (35 immunosup- fully amplified for PCP major An inflammatory cell population of pressed patients clinically sus- surface glycoprotein PCR after BAL fluid is an important aspect of picious for PCP and 20 im- DNA extraction, while control munocompetent individuals cytologic evaluation and can be used slides (n = 5) did not show am- not suspicious for PCP) were plification. to http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Cytologica Karger

Role of Cytology and Polymerase Chain Reaction Based Detection of Pneumocystis jirovecii Infection in Bronchoalveolar Lavage Fluid

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Publisher
Karger
Copyright
© 2011 S. Karger AG, Basel
ISSN
0001-5547
eISSN
1938-2650
DOI
10.1159/000325038
Publisher site
See Article on Publisher Site

Abstract

Molecular Techniques Role of Cytology and Polymerase Chain Reaction Based Detection of Pneumocystis jirovecii Infection in Bronchoalveolar Lavage Fluid Rashmi Gupta, M.Sc., Venkateswaran K. Iyer, M.D., Bijay Ranjan Mirdha, M.D., Randeep Guleria, M.D., Lalit Kumar, M.D., and Sanjay Kumar Agarwal, M.D. Objective croscopy for PCP, while 5 (including 2 microscopy positive) To study the role of cytology and polymerase chain reaction were positive by PCR from BALF. Four of the 5 positive (PCR) for the detection of Pneumocystis carinii pneumo- cases showed predominantly alveolar macrophages, while nia (PCP) in bronchoalveolar lavage fluid (BALF). one showed 40% alveolar macrophages, 30% lymphocytes and 20% neutrophils upon cellular analysis of BALF. One Study Design case had coinfection with Cryptococcus. Destained smear In a prospective observational scrapings of PCR-positive study, a BALF pellet from 55 slides (n = 5) were all success- patients (35 immunosup- fully amplified for PCP major An inflammatory cell population of pressed patients clinically sus- surface glycoprotein PCR after BAL fluid is an important aspect of picious for PCP and 20 im- DNA extraction, while control munocompetent individuals cytologic evaluation and can be used slides (n = 5) did not show am- not suspicious for PCP) were plification. to

Journal

Acta Cytologica – Karger

Published: Jan 1, 2011

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