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Quantification of Platelet-Associated IgG with Competitive Solid-Phase Enzyme Immunoassay

Quantification of Platelet-Associated IgG with Competitive Solid-Phase Enzyme Immunoassay In order to measure platelet-associated IgG (PAIgG), we devised a solidphase enzyme immunoassay employing a competitive binding of peroxidase-conjugated anti-IgG antiserum between platelets and polystyrene tubes coated with IgG. The amounts of peroxidase bound to the tubes were measured in a spectrophotometer by an enzymatic reaction. This method is highly sensitive, reproducible and can be carried out more simply. The PAIgG values of normal controls averaged 21.6 ± 6.6 (SD) ng/l0<sup>τ</sup> platelets. 27 (93%) of 29 patients with idiopathic thrombocytopenic purpura (ITP), who had a platelet count of less than 15×10<sup>4</sup>/μl, had PAIgG values greater than those of controls by 2 SD and averaged 205.5 ± 323 ng. There was a significant inverse correlation between platelet count and PAIgG value of ITP patients. The PAIgG values of patients with aplastic anemia were within normal range. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Haematologica Karger

Quantification of Platelet-Associated IgG with Competitive Solid-Phase Enzyme Immunoassay

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Publisher
Karger
Copyright
© 1981 S. Karger AG, Basel
ISSN
0001-5792
eISSN
1421-9662
DOI
10.1159/000207130
Publisher site
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Abstract

In order to measure platelet-associated IgG (PAIgG), we devised a solidphase enzyme immunoassay employing a competitive binding of peroxidase-conjugated anti-IgG antiserum between platelets and polystyrene tubes coated with IgG. The amounts of peroxidase bound to the tubes were measured in a spectrophotometer by an enzymatic reaction. This method is highly sensitive, reproducible and can be carried out more simply. The PAIgG values of normal controls averaged 21.6 ± 6.6 (SD) ng/l0<sup>τ</sup> platelets. 27 (93%) of 29 patients with idiopathic thrombocytopenic purpura (ITP), who had a platelet count of less than 15×10<sup>4</sup>/μl, had PAIgG values greater than those of controls by 2 SD and averaged 205.5 ± 323 ng. There was a significant inverse correlation between platelet count and PAIgG value of ITP patients. The PAIgG values of patients with aplastic anemia were within normal range.

Journal

Acta Haematologica – Karger

Published: Jan 1, 1981

Keywords: Thrombocytopenia; Enzyme immunoassay; Platelet-associated IgG

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