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Methods of Cytologic Smear Preparation and Fixation

Methods of Cytologic Smear Preparation and Fixation Acta Cytologica Methods of Cytologic Smear Preparation and Fixation Effect on the Immunoreactivity of Commonly Used Anticytokeratin Antibody AE1/AE3 Vinod B. Shidham, M.D., F.I.A.C., M.R.C.Path., Paul F. Lindholm, M.D., André Kajdacsy-Balla, M.D., Ph.D., Chung-Che Chang, M.D., Ph.D., and Richard Komorowski, M.D. OBJECTIVE: To evaluate the effect of fixation and meth- ther postfixed after rehydration in saline or fixed directly ods of cytologic smear preparation on the immunoreac- without rehydration by one of the three fixatives: alco- tivity of commonly used holic formalin, 95% ethanol anticytokeratin antibody with 5% acetic acid or 95% AE1/AE3. ethanol. AE1/AE3 demonstrated STUDY DESIGN: Scrape RESULTS: Both intensity immunochemistry results comparable cytology smears and forma- and proportion scores were lin-fixed, paraffin-embedded to those of FPTS if air-dried smears higher with rehydrated, air- tissue sections (FPTS) of 20 dried smears as compared to were rehydrated in saline and unfixed, fresh specimens those without rehydration postfixed in alcoholic formalin. submitted for intraoperative and were comparable to those consultation were studied with wet-fixed smears and by the immunoperoxidase FPTS. In the rehydrated method. In addition to the morphologic examination, the group, the optimum results were achieved when the smears and FPTS were evaluated for intensity and pro- smears http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Cytologica Karger

Methods of Cytologic Smear Preparation and Fixation

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Publisher
Karger
Copyright
© 2011 S. Karger AG, Basel
ISSN
0001-5547
eISSN
1938-2650
DOI
10.1159/000328590
Publisher site
See Article on Publisher Site

Abstract

Acta Cytologica Methods of Cytologic Smear Preparation and Fixation Effect on the Immunoreactivity of Commonly Used Anticytokeratin Antibody AE1/AE3 Vinod B. Shidham, M.D., F.I.A.C., M.R.C.Path., Paul F. Lindholm, M.D., André Kajdacsy-Balla, M.D., Ph.D., Chung-Che Chang, M.D., Ph.D., and Richard Komorowski, M.D. OBJECTIVE: To evaluate the effect of fixation and meth- ther postfixed after rehydration in saline or fixed directly ods of cytologic smear preparation on the immunoreac- without rehydration by one of the three fixatives: alco- tivity of commonly used holic formalin, 95% ethanol anticytokeratin antibody with 5% acetic acid or 95% AE1/AE3. ethanol. AE1/AE3 demonstrated STUDY DESIGN: Scrape RESULTS: Both intensity immunochemistry results comparable cytology smears and forma- and proportion scores were lin-fixed, paraffin-embedded to those of FPTS if air-dried smears higher with rehydrated, air- tissue sections (FPTS) of 20 dried smears as compared to were rehydrated in saline and unfixed, fresh specimens those without rehydration postfixed in alcoholic formalin. submitted for intraoperative and were comparable to those consultation were studied with wet-fixed smears and by the immunoperoxidase FPTS. In the rehydrated method. In addition to the morphologic examination, the group, the optimum results were achieved when the smears and FPTS were evaluated for intensity and pro- smears

Journal

Acta Cytologica – Karger

Published: Jan 1, 2011

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