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/lp/karger/lamp-1-is-upregulated-in-human-glioblastoma-cell-lines-induced-to-2rzWPDMq5M
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- Publisher
- Karger
- Copyright
- © 2001 National Science Council, ROC and S. Karger AG, Basel
- ISSN
- 1021-7770
- eISSN
- 1423-0127
- DOI
- 10.1159/000054056
- Publisher site
- See Article on Publisher Site
Abstract
Lysosome-associated membrane protein (LAMP)-1, one of the major protein components of the lysosomal membrane, is upregulated in the human glioblastoma cell lines, U-373 MG and LN-Z308, which undergo cisplatin-induced apoptosis. These human brain tumor cell lines demonstrated apoptosis in response to cisplatin/nifedipine treatment. Both cell lines demonstrated an apoptotic response by more than one criterion. Apoptosis was demonstrated by DNA fragmentation techniques such as DNA laddering, ApopTag<sup>®</sup> in situ labeling, and an ELISA-based method of detecting liberated oligosomes. These cells also had characteristic morphologic changes and upregulation of bax consistent with apoptosis. LAMP-1 expression at the protein and mRNA level was examined and found to increase with cisplatin/nifedipine treatment. LAMP-1 expression was examined using indirect immunofluorescent staining, Northern blot analysis and Western blot analysis. The finding of an augmentation of LAMP-1 in these cells induced to die is enigmatic. These findings raise the possibility of LAMP-1 involvement in the apoptotic process.
Journal
Journal of Biomedical Science – Karger
Published: Aug 1, 2001
Keywords: Apoptosis; Brain tumor; Cisplatin; LAMP; Lysosome