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In vitro Chick Pre-Cardiac Explant Tissue Differentiation during Spaceflight on SpaceHab-02

In vitro Chick Pre-Cardiac Explant Tissue Differentiation during Spaceflight on SpaceHab-02 Chick precardiac tissue explants were cultured on the 8-day mission of STS-60, space shuttle Discovery. Development of in vitro cultures of precardiac chick tissue from embryo stages 5 through 8 (H-H) were initiated during orbit and were terminated after approximately fifteen hours of 37 °C culture. Transmission electron microscopy and tritiated thymidine studies were performed postflight. No significant differences in cell proliferation were observed between flight and ground controls. Electron-microscopic studies revealed stage 8 explants were capable of differentiation during flight in a pattern which matched ground control tissues. As anticipated, stage 7 explant tissues had differentiated to a lesser extent compared to stage 8 tissues. Interestingly, stage 7 precardiac explant flight tissue differentiation was less than ground control tissue. This difference in differentiation between flight and ground cultures was enhanced in stage 6 tissues, as high levels of myofibril organization were only seen in ground controls. Other cellular components such as Golgi apparatus, junctional complexes, and mitochondria were present and appeared normal and healthy. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Cells Tissues Organs Karger

In vitro Chick Pre-Cardiac Explant Tissue Differentiation during Spaceflight on SpaceHab-02

Cells Tissues Organs , Volume 154 (3): 12 – Jan 1, 1995

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Publisher
Karger
Copyright
© 1995 S. Karger AG, Basel
ISSN
1422-6405
eISSN
1422-6421
DOI
10.1159/000147766
Publisher site
See Article on Publisher Site

Abstract

Chick precardiac tissue explants were cultured on the 8-day mission of STS-60, space shuttle Discovery. Development of in vitro cultures of precardiac chick tissue from embryo stages 5 through 8 (H-H) were initiated during orbit and were terminated after approximately fifteen hours of 37 °C culture. Transmission electron microscopy and tritiated thymidine studies were performed postflight. No significant differences in cell proliferation were observed between flight and ground controls. Electron-microscopic studies revealed stage 8 explants were capable of differentiation during flight in a pattern which matched ground control tissues. As anticipated, stage 7 explant tissues had differentiated to a lesser extent compared to stage 8 tissues. Interestingly, stage 7 precardiac explant flight tissue differentiation was less than ground control tissue. This difference in differentiation between flight and ground cultures was enhanced in stage 6 tissues, as high levels of myofibril organization were only seen in ground controls. Other cellular components such as Golgi apparatus, junctional complexes, and mitochondria were present and appeared normal and healthy.

Journal

Cells Tissues OrgansKarger

Published: Jan 1, 1995

Keywords: Heart; Development; Spaceflight; Differentiation; Proliferation; Chick

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