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Failure to Detect Genomic Material of HTLV-I or HTLV-ll in Mononuclear Cells of Italian Patients with Multiple Sclerosis and Chronic Progressive Myelopathy

Failure to Detect Genomic Material of HTLV-I or HTLV-ll in Mononuclear Cells of Italian Patients... To contribute to the undecided question if a retrovirus of the human T-cell lymphotropic virus (HTLV) family may be involved in the development of multiple sclerosis (MS), we investigated by the polymerase chain reaction (PCR) the presence of HTLV-I and HTLV-II sequences in the peripheral blood mononuclear cell DNAs from 30 patients affected by MS and 15 by chronic progressive myelopathy. Moreover a control group of 14 blood donors was examined. All these patients were devoid of anti-HTLV-I antibody in the serum and cerebrospinal fluid at ELISA. For the PCR, primers and probes specific for the tax region common to HTLV-I and HTLV-II, for the pol region of HTLV-I, and for the pol region of HTLV-II were used. In spite of the high sensitivity of the technique used, the three groups of subjects were negative for HTLV-I and HTLV-II genomic sequences. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png European Neurology Karger

Failure to Detect Genomic Material of HTLV-I or HTLV-ll in Mononuclear Cells of Italian Patients with Multiple Sclerosis and Chronic Progressive Myelopathy

Merelli, E.; Sola, P.; Marasco, R.; Salati, R.; Torelli, G.
European Neurology , Volume 33 (1): 4 – Jan 1, 1993
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Publisher
Karger
Copyright
© 1993 S. Karger AG, Basel
ISSN
0014-3022
eISSN
1421-9913
DOI
10.1159/000116894
Publisher site
See Article on Publisher Site

Abstract

To contribute to the undecided question if a retrovirus of the human T-cell lymphotropic virus (HTLV) family may be involved in the development of multiple sclerosis (MS), we investigated by the polymerase chain reaction (PCR) the presence of HTLV-I and HTLV-II sequences in the peripheral blood mononuclear cell DNAs from 30 patients affected by MS and 15 by chronic progressive myelopathy. Moreover a control group of 14 blood donors was examined. All these patients were devoid of anti-HTLV-I antibody in the serum and cerebrospinal fluid at ELISA. For the PCR, primers and probes specific for the tax region common to HTLV-I and HTLV-II, for the pol region of HTLV-I, and for the pol region of HTLV-II were used. In spite of the high sensitivity of the technique used, the three groups of subjects were negative for HTLV-I and HTLV-II genomic sequences.

Journal

European Neurology – Karger

Published: Jan 1, 1993

Keywords: Human T-cell lymphotropic virus type II; Multiple sclerosis; Chronic progressive myelopathy; Polymerase chain reaction; Human T-cell lymphotropic virus type I

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