Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Effect of Human Milk Fortifier on the Immunodetection and Molecular Mass Profile of Transforming Growth Factor-Alpha

Effect of Human Milk Fortifier on the Immunodetection and Molecular Mass Profile of Transforming... Objective: To determine if the addition of human milk fortifier (HMF) affects the distribution, immunoreactivity, or molecular mass profile of transforming growth factor-α (TGF-α) within the compartments of human milk. Methods: Fifteen milk samples were obtained. Each sample was divided into two aliquots; a powdered HMF was added to the first aliquot. TGF-α concentration was measured via radioimmunoassay in whole milk and its aqueous and fat fractions ± HMF. TGF-α molecular mass profiles of the samples (v/v) were measured via Western blotting. Results: TGF-α concentration (mean ± SD) in fortified whole milk (15.7 ± 7.1 pg/100 μl) vs. nonfortified whole milk (14.8 ± 8.0 pg/100 μl) and in the aqueous fraction of fortified (14.0 ± 2.7 pg/100 μl) vs. nonfortified (14.0 ± 3.5 pg/100 μl) did not differ statistically. There was, however, a marked decrease in the concentration of TGF-α in the fat fraction of fortified (30.6 ± 2.8 pg/100 μl) vs. nonfortified (98.0 ± 6.9 pg/100 μl) milk samples. Western blot for TGF-α in whole milk and its separated fractions revealed characteristic bands at 6.5, 12–16, 22, 26–30 and 46 kD. HMF alone and HMF with sodium taurocholate had a prominent band at 18 kD and fainter bands at 6.5, 26–30, and 46 kD. While whole and aqueous milk samples with HMF also consistently showed the 18-kD band, in 8/15 fat fraction samples with HMF the 18-kD band was nondetectable and was only faintly detectable in the remaining 7/15 samples. Conclusions: It appears that HMF differentially alters the biochemical profile of human milk with regard to TGF-α concentration and molecular mass profile. What effect this alteration in human milk biochemistry has on neonatal gut function remains unknown. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Neonatology Karger

Effect of Human Milk Fortifier on the Immunodetection and Molecular Mass Profile of Transforming Growth Factor-Alpha

Lessaris, Karen J.; Forsythe, Donna W.; Wagner, Carol L.
Neonatology , Volume 77 (3): 6 – Mar 1, 2000
Download PDF
6 pages

Loading next page...
 
/lp/karger/effect-of-human-milk-fortifier-on-the-immunodetection-and-molecular-05NEmNENgz

References

References for this paper are not available at this time. We will be adding them shortly, thank you for your patience.

Publisher
Karger
Copyright
© 2000 S. Karger AG, Basel
ISSN
1661-7800
eISSN
1661-7819
DOI
10.1159/000014210
Publisher site
See Article on Publisher Site

Abstract

Objective: To determine if the addition of human milk fortifier (HMF) affects the distribution, immunoreactivity, or molecular mass profile of transforming growth factor-α (TGF-α) within the compartments of human milk. Methods: Fifteen milk samples were obtained. Each sample was divided into two aliquots; a powdered HMF was added to the first aliquot. TGF-α concentration was measured via radioimmunoassay in whole milk and its aqueous and fat fractions ± HMF. TGF-α molecular mass profiles of the samples (v/v) were measured via Western blotting. Results: TGF-α concentration (mean ± SD) in fortified whole milk (15.7 ± 7.1 pg/100 μl) vs. nonfortified whole milk (14.8 ± 8.0 pg/100 μl) and in the aqueous fraction of fortified (14.0 ± 2.7 pg/100 μl) vs. nonfortified (14.0 ± 3.5 pg/100 μl) did not differ statistically. There was, however, a marked decrease in the concentration of TGF-α in the fat fraction of fortified (30.6 ± 2.8 pg/100 μl) vs. nonfortified (98.0 ± 6.9 pg/100 μl) milk samples. Western blot for TGF-α in whole milk and its separated fractions revealed characteristic bands at 6.5, 12–16, 22, 26–30 and 46 kD. HMF alone and HMF with sodium taurocholate had a prominent band at 18 kD and fainter bands at 6.5, 26–30, and 46 kD. While whole and aqueous milk samples with HMF also consistently showed the 18-kD band, in 8/15 fat fraction samples with HMF the 18-kD band was nondetectable and was only faintly detectable in the remaining 7/15 samples. Conclusions: It appears that HMF differentially alters the biochemical profile of human milk with regard to TGF-α concentration and molecular mass profile. What effect this alteration in human milk biochemistry has on neonatal gut function remains unknown.

Journal

Neonatology – Karger

Published: Mar 1, 2000

Keywords: Breast milk; Human milk fortifier; Transforming growth factor-α

There are no references for this article.