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/lp/karger/determination-of-prothrombin-by-a-micro-coagulation-method-vPwbTppErT
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- Publisher
- Karger
- Copyright
- © 1983 S. Karger AG, Basel
- ISSN
- 1424-8832
- eISSN
- 1424-8840
- DOI
- 10.1159/000214697
- Publisher site
- See Article on Publisher Site
Abstract
In contrast to earlier assays, the prothrombin determination described here uses a two-step reaction: first, prothrombin is activated with human factor Xa, phospholipids, Ca<sup>++</sup> and factor V; second, the amount of activated prothrombin is assayed by the proteolysis of added fibrinogen. The assay is dependent only on prothrombin, and independent of other components of thrombin generation. Carboxy- and decarboxyprothrombin can be differentiated by means of using Echis carinatus venom instead of factor Xa as activator. The micro-coagulation assay is compared with a conventional one-stage coagulation test, with a prothrombin determination using chromogenic substrate and immunologically by Laurell electrophoresis.
Journal
Pathophysiology of Haemostasis and Thrombosis – Karger
Published: Jan 1, 1983
Keywords: Prothrombin determination; Micro-coagulation assay; Thrombin generation; Anticoagulant therapy