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Detection of DNA Methylation

Detection of DNA Methylation MOLECULAR TECHNIQUES Acta Cytologica Potential Applications to Diagnostic Cytopathology Robert T. Pu, M.D., Ph.D., and Douglas P. Clark, M.D. Inactivation of tumor suppressor genes is often the result ration biopsies of tumors. (Acta Cytol 2003;47:247– of genetic mutations or deletions. However, another 252) mechanism for silencing genes involves DNA methyl- Keywords: DNA methyl- ation. In this setting, a ation, cancer, laboratory Although the application of MSP is methyl group is added to cy- diagnosis. limited to date, future studies may tosine residues within the reveal an important adjunctive role gene promoter region; that DNA methylation is a prevents transcription. Tu- mechanism used by cells in cancer screening, diagnosis and mors usually contain multi- to regulate gene expres- therapeutic monitoring with ple genes that have been si- sion. It involves the addi- lenced by methylation, tion of a methyl group to cytologic specimens. unlike normal tissues, in cytosine nucleotides by which gene methylation enzymes called DNA 1,2 events are less common. The list of genes methylated in a methyltransferases in eukaryotes. Typically, the given tumor is often referred to as a gene methylation methylated cytosines are clustered in chromosomal profile. Gene methylation profiles may be almost unique regions rich in http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Cytologica Karger

Detection of DNA Methylation

Pu, Robert T.; Clark, Douglas P.
Acta Cytologica , Volume 47 (2): 6 – Jan 1, 2011
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References (24)

Publisher
Karger
Copyright
© 2011 S. Karger AG, Basel
ISSN
0001-5547
eISSN
1938-2650
DOI
10.1159/000326511
Publisher site
See Article on Publisher Site

Abstract

MOLECULAR TECHNIQUES Acta Cytologica Potential Applications to Diagnostic Cytopathology Robert T. Pu, M.D., Ph.D., and Douglas P. Clark, M.D. Inactivation of tumor suppressor genes is often the result ration biopsies of tumors. (Acta Cytol 2003;47:247– of genetic mutations or deletions. However, another 252) mechanism for silencing genes involves DNA methyl- Keywords: DNA methyl- ation. In this setting, a ation, cancer, laboratory Although the application of MSP is methyl group is added to cy- diagnosis. limited to date, future studies may tosine residues within the reveal an important adjunctive role gene promoter region; that DNA methylation is a prevents transcription. Tu- mechanism used by cells in cancer screening, diagnosis and mors usually contain multi- to regulate gene expres- therapeutic monitoring with ple genes that have been si- sion. It involves the addi- lenced by methylation, tion of a methyl group to cytologic specimens. unlike normal tissues, in cytosine nucleotides by which gene methylation enzymes called DNA 1,2 events are less common. The list of genes methylated in a methyltransferases in eukaryotes. Typically, the given tumor is often referred to as a gene methylation methylated cytosines are clustered in chromosomal profile. Gene methylation profiles may be almost unique regions rich in

Journal

Acta Cytologica – Karger

Published: Jan 1, 2011

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